LOCUS       sequence-474286-       11189 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This vector features a tetracycline-inducible system to regulate 
            luxABCDE reporter expression, optimized for efficiency in P. 
            aeruginosa..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 871 to 905)
  AUTHORS   Zhang Y, Zhang T, Xiao X, Wang Y, Kawalek A, Ou J, Ren A, Sun W, de 
            Bakker V, Liu Y, Li Y, Yang L, Ye L, Jia N, Veening JW, Liu X
  TITLE     CRISPRi screen identifies FprB as a synergistic target for gallium 
            therapy in Pseudomonas aeruginosa.
  JOURNAL   Nat Commun. 2025 Jul 1;16(1):5870. doi: 10.1038/s41467-025-61208-z.
  PUBMED    40595632
REFERENCE   2  (bases 1 to 11189)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-07-1"; volume: "16(1):5870. doi"; pages: " 
            10.1038/s41467-025-61208-z"
COMMENT     Sequence Label: ptet-lux-Tn7T-Gm
FEATURES             Location/Qualifiers
     source          1..11189
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    12..59
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        complement(871..905)
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     promoter        join(1019..1037,1038..1043,1044..1060,1061..1066,
                     1067..1092)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    1019..1037
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1044..1062
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             1107..2549
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             2562..3485
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             3534..4616
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             4631..5614
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             5793..6905
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     mobile_element  7003..7168
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     oriT            7327..7435
                     /note="incP origin of transfer"
     rep_origin      7706..8062
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             complement(join(8215..9006,9007..9075))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8838..8857
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9076..9180)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9248..9266
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9304..9326)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9426..9445
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9859..9875
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     9860..9876
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      9904..9998
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      10101..10187
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    10233..10280
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(10410..10943)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(11132..11137,11138..11154,11155..11160))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 caggggatct tgaagttcct attccgaagt tcctattctc tagaaagtat aggaacttca
       61 gagcgctttt gaagctaatt cgatcatgca tgagctcact agtggatcca aaaaattagc
      121 gcaagaagac aaaaatcacc ttgcgctaat gctctgttac agtggagctc gcgaaaaaac
      181 cccgccgaag cggggttttt tgcgtaattt tcctcctatt tagatcctta ggagccggat
      241 tcgcatttca gctgcttctc gaggccgcag atgatgagct cgaggccgaa gaggaaggcc
      301 ggctccgcgc cctgatgatc gaagagctcg atcgcttggc ggaggagcgg gggcatggaa
      361 tccgtggtcg gcgtttcgcg ttcctctttg gcgacctgat gctcttggtc ctccagcacg
      421 cagcccaggg tgaaatgccc cacggcggag agcgcataca gcgcattttc gaggctgaac
      481 ccctgttggc agaggaacgc cagttggttt tcgagcgtct catattgctt ctcggtcggg
      541 cgggtgccga gatggacctt ggccccatcg cgatgggaga ggagcgcgca gcggaaggac
      601 ttcgcattat tccggaggaa atcctgccac gattccccct cgagcgggca gaagtgcgtg
      661 tgatggcggt cgagcatctc gatggccagg gcatccagga gggcgcgttt atttttgaca
      721 tgccaataca gggtgggctg ctcgaccccg agcttctggg cgagtttgcg ggtggtcagg
      781 ccctcgatgc cgacttcatt gaggagttcc agcgcgctgt tgatcacctt gcttttatcg
      841 agccggctca tcgaggcctt tcctgtgtga gctagcactg tacctaggac tgagctagcc
      901 gtcaattggg atccgatatc tagatgcatt cgcgaggtac cgagctcgaa ttccgcaaaa
      961 aaccccgctt cggcggggtt ttttcgcgga tctagacggt gatcaacacg tcactagttc
     1021 cctatcagtg atagagattg acatccctat cagtgataga gatactgagc acatcagcag
     1081 gacgcactga ccaggaggta caatcaatga ctaaaaaaat ttcattcatt attaacggcc
     1141 aggttgaaat ctttcccgaa ggtgatgatt tagtgcaatc cattaatttt ggtgataata
     1201 gtgtttacct gccaatattg aatgactctc atgtaaaaaa cattattgat tgtaatggaa
     1261 ataacgaatt acggttgcat aacattgtca attttctcta tacggtaggg caaagatgga
     1321 aaaatgaaga atactcaaga cgcaggacat acattcgtga cttaaaaaaa tatatgggat
     1381 attcagaaga aatggctaag ctagaggcca attggatatc tatgatttta tgttctaaag
     1441 gcggccttta tgatgttgta gaaaatgaac ttggttctcg ccatatcatg gatgaatggc
     1501 tacctcagga tgaaagttat gttcgggctt ttccgaaagg taaatctgta catctgttgg
     1561 caggtaatgt tccattatct gggatcatgt ctatattacg cgcaatttta actaagaatc
     1621 agtgtattat aaaaacatcg tcaaccgatc cttttaccgc taatgcatta gcgttaagtt
     1681 ttattgatgt agaccctaat catccgataa cgcgctcttt atctgttata tattggcccc
     1741 accaaggtga tacatcactc gcaaaagaaa ttatgcaaca tgcggatgtt attgtcgctt
     1801 ggggagggcc agatgcgatt aattgggcgg tagagcatgc gccatcttat gctgatgtga
     1861 ttaaatttgg ttctaaaaag agtctttgca ttatcgataa tcctgttgat ttgacgtccg
     1921 cagcgacagg tgcggctcat gatgtttgtt tttacgatca gcgagcttgt ttttctgccc
     1981 aaaacatata ttacatggga aatcattatg aggaatttaa gttagcgttg atagaaaaac
     2041 ttaatctata tgcgcatata ttaccgaatg ccaaaaaaga ttttgatgaa aaggcggcct
     2101 attctttagt tcaaaaagaa agcttgtttg ctggattaaa agtagaggtg gatattcatc
     2161 aacgttggat gattattgag tcaaatgcag gtgtggaatt taatcaacca cttggcagat
     2221 gtgtgtacct tcatcacgtc gataatattg agcaaatatt gccttatgtt caaaaaaata
     2281 agacgcaaac catatctatt tttccttggg agtcatcatt taaatatcga gatgcgttag
     2341 cattaaaagg tgcggaaagg attgtagaag caggaatgaa taacatattt cgagttggtg
     2401 gatctcatga cggaatgaga ccgttgcaac gattagtgac atatatttct catgaaaggc
     2461 catctaacta tacggctaag gatgttgcgg ttgaaataga acagactcga ttcctggaag
     2521 aagataagtt ccttgtattt gtcccataat aggtaaaaag tatggaaaat gaatcaaaat
     2581 ataaaaccat cgaccacgtt atttgtgttg aaggaaataa aaaaattcat gtttgggaaa
     2641 cgctgccaga agaaaacagc ccaaagagaa agaatgccat tattattgcg tctggttttg
     2701 cccgcaggat ggatcatttt gctggtctgg cggaatattt atcgcggaat ggatttcatg
     2761 tgatccgcta tgattcgctt caccacgttg gattgagttc agggacaatt gatgaattta
     2821 caatgtctat aggaaagcag agcttgttag cagtggttga ttggttaact acacgaaaaa
     2881 taaataactt cggtatgttg gcttcaagct tatctgcgcg gatagcttat gcaagcctat
     2941 ctgaaatcaa tgcttcgttt ttaatcaccg cagtcggtgt tgttaactta agatattctc
     3001 ttgaaagagc tttagggttt gattatctca gtctacccat taatgaattg ccgaataatc
     3061 tagattttga aggccataaa ttgggtgctg aagtctttgc gagagattgt cttgattttg
     3121 gttgggaaga tttagcttct acaattaata acatgatgta tcttgatata ccgtttattg
     3181 cttttactgc aaataacgat aattgggtca agcaagatga agttatcaca ttgttatcaa
     3241 atattcgtag taatcgatgc aagatatatt ctttgttagg aagttcgcat gacttgagtg
     3301 aaaatttagt ggtcctgcgc aatttttatc aatcggttac gaaagccgct atcgcgatgg
     3361 ataatgatca tctggatatt gatgttgata ttactgaacc gtcatttgaa catttaacta
     3421 ttgcgacagt caatgaacgc cgaatgagaa ttgagattga aaatcaagca atttctctgt
     3481 cttaaaatct attgagatat tctatcactc aaatagcaat ataaggactc tctatgaaat
     3541 ttggaaactt tttgcttaca taccaacctc cccaattttc tcaaacagag gtaatgaaac
     3601 gtttggttaa attaggtcgc atctctgagg agtgtggttt tgataccgta tggttactgg
     3661 agcatcattt cacggagttt ggtttgcttg gtaaccctta tgtcgctgct gcatatttac
     3721 ttggcgcgac taaaaaattg aatgtaggaa ctgccgctat tgttcttccc acagcccatc
     3781 cagtacgcca acttgaagat gtgaatttat tggatcaaat gtcaaaagga cgatttcggt
     3841 ttggtatttg ccgagggctt tacaacaagg actttcgcgt attcggcaca gatatgaata
     3901 acagtcgcgc cttagcggaa tgctggtacg ggctgataaa gaatggcatg acagagggat
     3961 atatggaagc tgataatgaa catatcaagt tccataaggt aaaagtaaac cccgcggcgt
     4021 atagcagagg tggcgcaccg gtttatgtgg tggctgaatc agcttcgacg actgagtggg
     4081 ctgctcaatt tggcctaccg atgatattaa gttggattat aaatactaac gaaaagaaag
     4141 cacaacttga gctttataat gaagtggctc aagaatatgg gcacgatatt cataatatcg
     4201 accattgctt atcatatata acatctgtag atcatgactc aattaaagcg aaagagattt
     4261 gccggaaatt tctggggcat tggtatgatt cttatgtgaa tgctacgact atttttgatg
     4321 attcagacca aacaagaggt tatgatttca ataaagggca gtggcgtgac tttgtattaa
     4381 aaggacataa agatactaat cgccgtattg attacagtta cgaaatcaat cccgtgggaa
     4441 cgccgcagga atgtattgac ataattcaaa aagacattga tgctacagga atatcaaata
     4501 tttgttgtgg atttgaagct aatggaacag tagacgaaat tattgcttcc atgaagctct
     4561 tccagtctga tgtcatgcca tttcttaaag aaaaacaacg ttcgctatta tattagctaa
     4621 ggagaaagaa atgaaatttg gattgttctt ccttaacttc atcaattcaa caactgttca
     4681 agaacaaagt atagttcgca tgcaggaaat aacggagtat gttgataagt tgaattttga
     4741 acagatttta gtgtatgaaa atcatttttc agataatggt gttgtcggcg ctcctctgac
     4801 tgtttctggt tttctgctcg gtttaacaga gaaaattaaa attggttcat taaatcacat
     4861 cattacaact catcatcctg tccgcatagc ggaggaagct tgcttattgg atcagttaag
     4921 tgaagggaga tttattttag ggtttagtga ttgcgaaaaa aaagatgaaa tgcatttttt
     4981 taatcgcccg gttgaatatc aacagcaact atttgaagag tgttatgaaa tcattaacga
     5041 tgctttaaca acaggctatt gtaatccaga taacgatttt tatagcttcc ctaaaatatc
     5101 tgtaaatccc catgcttata cgccaggcgg acctcggaaa tatgtaacag caaccagtca
     5161 tcatattgtt gagtgggcgg ccaaaaaagg tattcctctc atctttaagt gggatgattc
     5221 taatgatgtt agatatgaat atgctgaaag atataaagcc gttgcggata aatatgacgt
     5281 tgacctatca gagatagacc atcagttaat gatattagtt aactataacg aagatagtaa
     5341 taaagctaaa caagagacgc gtgcatttat tagtgattat gttcttgaaa tgcaccctaa
     5401 tgaaaatttc gaaaataaac ttgaagaaat aattgcagaa aacgctgtcg gaaattatac
     5461 ggagtgtata actgcggcta agttggcaat tgaaaagtgt ggtgcgaaaa gtgtattgct
     5521 gtcctttgaa ccaatgaatg atttgatgag ccaaaaaaat gtaatcaata ttgttgatga
     5581 taatattaag aagtaccaca tggaatatac ctaatagatt tcgagttgca gcgaggcggc
     5641 aagtgaacga atccccagga gcatagataa ctatgtgact ggggtgagtg aaagcagcca
     5701 acaaagcagc agcttgaaag atgaagggta taaaagagta tgacagcagt gctgccatac
     5761 tttctaatat tatcttgagg agtaaaacag gtatgacttc atatgttgat aaacaagaaa
     5821 ttacagcaag ctcagaaatt gatgatttga ttttttcgag cgatccatta gtgtggtctt
     5881 acgacgagca ggaaaaaatc agaaagaaac ttgtgcttga tgcatttcgt aatcattata
     5941 aacattgtcg agaatatcgt cactactgtc aggcacacaa agtagatgac aatattacgg
     6001 aaattgatga catacctgta ttcccaacat cggtttttaa gtttactcgc ttattaactt
     6061 ctcaggaaaa cgagattgaa agttggttta ccagtagcgg cacgaatggt ttaaaaagtc
     6121 aggtggcgcg tgacagatta agtattgaga gactcttagg ctctgtgagt tatggcatga
     6181 aatatgttgg tagttggttt gatcatcaaa tagaattagt caatttggga ccagatagat
     6241 ttaatgctca taatatttgg tttaaatatg ttatgagttt ggtggaattg ttatatccta
     6301 cgacatttac cgtaacagaa gaacgaatag attttgttaa aacattgaat agtcttgaac
     6361 gaataaaaaa tcaagggaaa gatctttgtc ttattggttc gccatacttt atttatttac
     6421 tctgccatta tatgaaagat aaaaaaatct cattttctgg agataaaagc ctttatatca
     6481 taaccggagg cggctggaaa agttacgaaa aagaatctct gaaacgtgat gatttcaatc
     6541 atcttttatt tgatactttc aatctcagtg atattagtca gatccgagat atatttaatc
     6601 aagttgaact caacacttgt ttctttgagg atgaaatgca gcgtaaacat gttccgccgt
     6661 gggtatatgc gcgagcgctt gatcctgaaa cgttgaaacc tgtacctgat ggaacgccgg
     6721 ggttgatgag ttatatggat gcgtcagcaa ccagttatcc agcatttatt gttaccgatg
     6781 atgtcgggat aattagcaga gaatatggta agtatcccgg cgtgctcgtt gaaattttac
     6841 gtcgcgtcaa tacgaggacg cagaaagggt gtgctttaag cttaaccgaa gcgtttgata
     6901 gttgacacct gcctcgcgcc gcaaaccccg cccctgacag ggcggggttt cgccgctcct
     6961 cgagaagctt gggcccggta cctcgcgaag gccttgcagg ccaaccagat aagtgaaatc
     7021 tagttccaaa ctattttgtc atttttaatt ttcgtattag cttacgacgc tacacccagt
     7081 tcccatctat tttgtcactc ttccctaaat aatccttaaa aactccattt ccacccctcc
     7141 cagttcccaa ctattttgtc cgcccacagc ggggcatttt tcttcctgtt atgtttgggc
     7201 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     7261 cgcttcctcg ctcactgacc cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcaga
     7321 gcttatcggc cagcctcgca gagcaggatt cccgttgagc accgccaggt gcgaataagg
     7381 gacagtgaag aaggaacacc cgctcgcggg tgggcctact tcacctatcc tgcccggctg
     7441 acgccgttgg atacaccaag gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg
     7501 tgcgaaaaag gatggatata ccgaaaaaat cgctataatg accccgaagc agggttatgc
     7561 agcggaaagt ataccttaag gaatcccctg ataacgcagg aaagaacatg tgagcaaaag
     7621 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgttccta catacctcgc
     7681 tctgctaatc ctgttaccag ccggttgtca gccgttaagt gttcctgtgt cactcaaaat
     7741 tgctttgaga ggctctaagg gcttctcagt gcgttacatc cctggcttgt tgtccacaac
     7801 cgttaaacct taaaagcttt aaaagcctta tatattcttt tttttcttat aaaacttaaa
     7861 accttagagg ctatttaagt tgctgattta tattaatttt attgttcaaa catgagagct
     7921 tagtacgtga aacatgagag cttagtacgt tagccatgag agcttagtac gttagccatg
     7981 agggtttagt tcgttaaaca tgagagctta gtacgttaaa cttgagagct tagtacgtga
     8041 aacatgagag cttagtacgt actatcaaca ggttgaactg cccatgaacg aaaactcacg
     8101 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     8161 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     8221 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     8281 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     8341 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     8401 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     8461 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     8521 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     8581 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     8641 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     8701 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     8761 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     8821 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     8881 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     8941 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     9001 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     9061 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     9121 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     9181 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     9241 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg acgacggtga
     9301 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     9361 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     9421 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     9481 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
     9541 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctagagg accagccgcg
     9601 taacctggca aaatcggtta cggttgagta ataaatggat gccctgcgta agcaggtgtg
     9661 ggcggacaat aaagtcttaa actgaacaaa atagatctaa actatgacaa taaagtctta
     9721 aactagacag aatagttgta aactgaaatc agtccagtta tgctgtgaaa aagcatactg
     9781 gacttttgtt atggctaaag caaactcttc attttctgaa gtgcaaattg cccgtcgtat
     9841 taaagagggg cgtggggttc gaggtcgacg gtatcgataa gctagcttaa ttagctgagc
     9901 ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa
     9961 cgctcggttg ccgccgggcg ttttttattg gtgagaatcc aagctagact gcgatgagtg
    10021 gcagggcggg gcgtaatttt tttaaggcag ttattggtgc ccttaaacgc ctggggtaat
    10081 gactctctag cttgaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
    10141 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgctaggag
    10201 cttgcggccc ggacgatcga attggggatc ttgaagtacc tattccgaag ttcctattct
    10261 ctagaaagta taggaacttc agagcgcttt tgaagctgat gtgcttaaaa acttactcaa
    10321 tggaattcta gatcgaattg gccgcggcgt tgtgacaatt taccgaacaa ctccgcggcc
    10381 gggaagccga tctcggcttg aacgaattgt taggtggcgg tacttgggtc gatatcaaag
    10441 tgcatcactt cttcccgtat gcccaacttt gtatagagag ccactgcggg atcgtcaccg
    10501 taatctgctt gcacgtagat cacataagca ccaagcgcgt tggcctcatg cttgaggaga
    10561 ttgatgagcg cggtggcaat gccctgcctc cggtgctcgc cggagactgc gagatcatag
    10621 atatagatct cactacgcgg ctgctcaaac ttgggcagaa cgtaagccgc gagagcgcca
    10681 acaaccgctt cttggtcgaa ggcagcaagc gcgatgaatg tcttactacg gagcaagttc
    10741 ccgaggtaat cggagtccgg ctgatgttgg gagtaggtgg ctacgtctcc gaactcacga
    10801 ccgaaaagat caagagcagc ccgcatggat ttgacttggt cagggccgag cctacatgtg
    10861 cgaatgatgc ccatacttga gccacctaac tttgttttag ggcgactgcc ctgctgcgta
    10921 acatcgttgc tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc
    10981 gtaacgcgct tgctgcttgg atgcccgagg catagactgt acaaaaaaac agtcataaca
    11041 agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca
    11101 gttgcgtgag cgcatacgct acttgcatta cagtttacga accgaacagg cttatgtcaa
    11161 ttcgatccat tgctgttgac aaagggaat
//
