LOCUS       sequence-474288-       10375 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human WDR5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10375)
  AUTHORS   Sashiyama S, Nakagawa T, Nakagawa M, Hosogane M, Watanabe Y, 
            Ashitomi H, Yamane K, Shibuya N, Moroishi T, Nakayama K, Hosoi T
  TITLE     KBG syndrome-associated protein ANKRD11 regulates SETD5 expression 
            to modulate rRNA levels and translation.
  JOURNAL   iScience. 2025 May 19;28(6):112699. doi: 10.1016/j.isci.2025.112699.
            eCollection 2025 Jun 20.
  PUBMED    40520101
REFERENCE   2  (bases 1 to 10375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2025-05-19"; volume: "28(6):112699. doi"; pages: " 
            10.1016/j.isci.2025.112699. eCollection 2025 Jun 20"
COMMENT     Sequence Label: PB-TA-ERN-WDR5-FLAG
FEATURES             Location/Qualifiers
     source          1..10375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(28..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     350..369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     583..605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        621..639
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   706..768
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1140..1158
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1177..1195
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1214..1232
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1251..1269
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1288..1306
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1325..1343
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1362..1380
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1399..1417
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1439..1463
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1542..1566
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2638..2662)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2664..2687
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(2718..2736)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2719..2736)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2756..2773)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2762..2986
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        3083..3462
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3577..4882
                     /label=rEF-1-alpha promoter
                     /note="strong constitutive promoter for rat elongation 
                     factor EF-1-alpha"
     misc_feature    3919..4850
                     /label=rEF-1-alpha intron
                     /note="intron upstream of the start codon of rat 
                     EF-1-alpha"
     CDS             join(4899..5516,5517..5522,5523..5558,5559..5561,5562..5597,
                     5598..5600,5601..5636,5637..5645)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    5672..6116
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        6149..6195
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        6187..6205
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6215..7009
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6215..7009
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6269..6288)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6879..6898
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7056..7628
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
     repeat_region   8002..8036
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(8185..8203)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8224..8240)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8224..8240)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8237..8259)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8248..8264
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8272..8278,8279..8296,8297..8302))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8317..8338
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8455..8472)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8626..9214)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8706..8725)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9385..10176,10177..10245))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10008..10027
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10246..10350)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgaattg taatacgact cactataggg cgaattggag ctcggtattc
      661 acgacagcag gctgaataat aaaaaaatta gaaactatta tttaacccta gaaagataat
      721 catattgtga cgtacgttaa agataatcat gcgtaaaatt gacgcatgtg ttttatcggt
      781 ctgtatatcg aggtttattt attaatttga atagatatta agttttatta tatttacact
      841 tacatactaa taataaattc aacaaacaat ttatttatgt ttatttattt attaaaaaaa
      901 aacaaaaact caaaatttct tctataaagt aacaaaactt ttaaacattc tctcttttac
      961 aaaaataaac ttattttgta ctttaaaaac agtcatgttg tattataaaa taagtaatta
     1021 gcttaaccta tacataatag aaacaaatta tacttattag tcagtcagaa acaactttgg
     1081 cacatatcaa tattatgctc tcgttaatta actcgaggga tccaagctgg ccgcacgtct
     1141 ccctatcagt gatagagaag tcgacacgtc tcgagctccc tatcagtgat agagaaggta
     1201 cgtctagaac gtctccctat cagtgataga gaagtcgaca cgtctcgagc tccctatcag
     1261 tgatagagaa ggtacgtcta gaacgtctcc ctatcagtga tagagaagtc gacacgtctc
     1321 gagctcccta tcagtgatag agaaggtacg tctagaacgt ctccctatca gtgatagaga
     1381 agtcgacacg tctcgagctc cctatcagtg atagagaagg taccccctat ataagcagag
     1441 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     1501 gaagacaccg ggaccgatcc agcctccgcg gtggcggcat cacaagtttg tacaaaaaag
     1561 caggctcttt aaaggaacca attcagtcga ctggatccgg taccgaattc atggcgacgg
     1621 aggagaagaa gcccgagacc gaggccgcca gagcacagcc aaccccttcg tcatccgcca
     1681 ctcagagcaa gcctacacct gtgaagccaa actatgctct aaagttcacc cttgctggcc
     1741 acaccaaagc agtgtcctcc gtgaaattca gcccgaatgg agagtggctg gcaagttcat
     1801 ctgctgataa acttattaaa atttggggcg cgtatgatgg gaaatttgag aaaaccatat
     1861 ctggtcacaa gctgggaata tccgatgtag cctggtcgtc agattctaac cttcttgttt
     1921 ctgcctcaga tgacaaaacc ttgaagatat gggacgtgag ctcgggcaag tgtctgaaaa
     1981 ccctgaaggg acacagtaat tatgtctttt gctgcaactt caatccccag tccaacctta
     2041 ttgtctcagg atcctttgac gaaagcgtga ggatatggga tgtgaaaaca gggaagtgcc
     2101 tcaagacttt gccagctcac tcggatccag tctcggccgt tcattttaat cgtgatggat
     2161 ccttgatagt ttcaagtagc tatgatggtc tctgtcgcat ctgggacacc gcctcaggcc
     2221 agtgcctgaa gacgctcatc gatgacgaca acccccccgt gtcttttgtg aagttctccc
     2281 cgaacggcaa atacatcctg gccgccacgc tggacaacac tctgaagctc tgggactaca
     2341 gcaaggggaa gtgcctgaag acgtacactg gccacaagaa tgagaaatac tgcatatttg
     2401 ccaatttctc tgttactggt gggaagtgga ttgtgtctgg ctcagaggat aaccttgttt
     2461 acatctggaa ccttcagacg aaagagattg tacagaaact acaaggccac acagatgtcg
     2521 tgatctcaac agcttgtcac ccaacagaaa acatcatcgc ctctgctgcg ctagaaaatg
     2581 acaaaacaat taaactgtgg aagagtgact gctggcggcc gcactcgaga tatctagacc
     2641 cagctttctt gtacaaagtg gttgactaca aagacgatga cgacaagtga ctcgagcatg
     2701 catctagagg gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg
     2761 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     2821 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     2881 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     2941 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     3001 agaaccagat ctgtacattc gaatcgatga attcgttaac catggaagct tctgcagcgg
     3061 ccgcactagc ggtggcggcc tcgacattga ttattgacta gttattaata gtaatcaatt
     3121 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     3181 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     3241 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     3301 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     3361 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     3421 acttggcagt acatctacgt attagtcatc gctattacca tgatgatgcg gttttggcag
     3481 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     3541 gacgtcaatg ggagtttgtt ttgactagtg gagccgagag taattcatac aaaaggaggg
     3601 atcgccttcg caaggggaga gcccagggac cgtccctaaa ttctcacaga cccaaatccc
     3661 tgtagccgcc ccacgacagc gcgaggagca tgcgcccagg gctgagcgcg ggtagatcag
     3721 agcacacaag ctcacagtcc ccggcggtgg ggggaggggc gcgctgagcg ggggccaggg
     3781 agctggcgcg gggcaaactg ggaaagtggt gtcgtgtgct ggctccgccc tcttcccgag
     3841 ggtgggggag aacggtatat aagtgcggta gtcgccttgg acgttctttt tcgcaacggg
     3901 tttgccgtca gaacgcaggt gagtggcggg tgtggcttcc gcgggccccg gagctggagc
     3961 cctgctctga gcgggccggg ctgatatgcg agtgtcgtcc gcagggttta gctgtgagca
     4021 ttcccacttc gagtggcggg cggtgcgggg gtgagagtgc gaggcctagc ggcaaccccg
     4081 tagcctcgcc tcgtgtccgg cttgaggcct agcgtggtgt ccgccgccgc gtgccactcc
     4141 ggccgcacta tgcgtttttt gtccttgctg ccctcgattg ccttccagca gcatgggcta
     4201 acaaagggag ggtgtggggc tcactcttaa ggagcccatg aagcttacgt tggataggaa
     4261 tggaagggca ggaggggcga ctggggcccg cccgccttcg gagcacatgt ccgacgccac
     4321 ctggatgggg cgaggcctgt ggctttccga agcaatcggg cgtgagttta gcctacctgg
     4381 gccatgtggc cctagcactg ggcacggtct ggcctggcgg tgccgcgttc ccttgcctcc
     4441 caacaagggt gaggccgtcc cgcccggcac cagttgcttg cgcggaaaga tggccgctcc
     4501 cggggccctg ttgcaaggag ctcaaaatgg aggacgcggc agcccggtgg agcgggcggg
     4561 tgagtcaccc acacaaagga agagggcctt gcccctcgcc ggccgctgct tcctgtgacc
     4621 ccgtggtcta tcggccgcat agtcacctcg ggcttctctt gagcaccgct cgtcgcggcg
     4681 gggggagggg atctaatggc gttggagttt gttcacattt ggtgggtgga gactagtcag
     4741 gccagcctgg cgctggaagt cattcttgga atttgcccct ttgagtttgg agcgaggcta
     4801 attctcaagc ctcttagcgg ttcaaaggta ttttctaaac ccgtttccag gtgttgtgaa
     4861 agccaccgct aattcaaagc aatccggagt aattcaccat gtctagactg gacaagagca
     4921 aagtcataaa cggcgctctg gaattactca atggagtcgg tatcgaaggc ctgacgacaa
     4981 ggaaactcgc tcaaaagctg ggagttgagc agcctaccct gtactggcac gtgaagaaca
     5041 agcgggccct gctcgatgcc ctgccaatcg agatgctgga caggcatcat acccacttct
     5101 gccccctgga aggcgagtca tggcaagact ttctgcggaa caacgccaag tcattccgct
     5161 gtgctctcct ctcacatcgc gacggggcta aagtgcatct cggcacccgc ccaacagaga
     5221 aacagtacga aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc ttctccctgg
     5281 agaacgcact gtacgctctg tccgccgtgg gccactttac actgggctgc gtattggagg
     5341 aacaggagca tcaagtagca aaagaggaaa gagagacacc taccaccgat tctatgcccc
     5401 cacttctgag acaagcaatt gagctgttcg accggcaggg agccgaacct gccttccttt
     5461 tcggcctgga actaatcata tgtggcctgg agaaacagct aaagtgcgaa agcggcgggc
     5521 cggccgacgc ccttgacgat tttgacttag acatgctccc agccgatgcc cttgacgact
     5581 ttgaccttga tatgctgcct gctgacgctc ttgacgattt tgaccttgac atgctccccg
     5641 ggtaactaag taaggatccc gtacgcctag gagcaggttt ccccaatgac acaaaacgtg
     5701 caacttgaaa ctccgcctgg tctttccagg tctagagggg taacactttg tactgcgttt
     5761 ggctccacgc tcgatccact ggcgagtgtt agtaacagca ctgttgcttc gtagcggagc
     5821 atgacggccg tgggaactcc tccttggtaa caaggaccca cggggccaaa agccacgccc
     5881 acacgggccc gtcatgtgtg caaccccagc acggcgactt tactgcgaaa cccactttaa
     5941 agtgacattg aaactggtac ccacacactg gtgacaggct aaggatgccc ttcaggtacc
     6001 ccgaggtaac acgcgacact cgggatctga gaaggggact ggggcttcta taaaagcgct
     6061 cggtttaaaa agcttctatg cctgaatagg tgaccggagg tcggcacctt tcctttgcaa
     6121 ttactgaccc tatgaataca ctgactgttt gacaattaat catcggcata gtatatcggc
     6181 atagtataat acgactcact ataggagggc caccatgatt gaacaagatg gattgcacgc
     6241 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     6301 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     6361 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg
     6421 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     6481 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     6541 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     6601 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     6661 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     6721 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacacatgg
     6781 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     6841 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     6901 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     6961 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     7021 gggttcgaaa tgaccgacca agcgaattcg ctaggattat ccctaatacc tgccacccca
     7081 ctcttaatca gtggtggaag aacggtctca gaactgtttg tttcaattgg ccatttaagt
     7141 ttagtagtaa aagactggtt aatgataaca atgcatcgta aaaccttcag aaggaaagga
     7201 gaatgttttg tggaccactt tggttttctt ttttgcgtgt ggcagtttta agttattagt
     7261 ttttaaaatc agtacttttt aatggaaaca acttgaccaa aaatttgtca cagaattttg
     7321 agacccatta aaaaagttaa atgagaaacc tgtgtgttcc tttggtcaac accgagacat
     7381 ttaggtgaaa gacatctaat tctggtttta cgaatctgga aacttcttga aaatgtaatt
     7441 cttgagttaa cacttctggg tggagaatag ggttgttttc cccccacata attggaaggg
     7501 gaaggaatat catttaaagc tatgggaggg ttgctttgat tacaacactg gagagaaatg
     7561 cagcatgttg ctgattgcct gtcactaaaa caggccaaaa actgagtcct tgggttgcat
     7621 agaaagctgc caaacgctag ccaacaagct cgtcatcgct ttgcagaaga gcagagagga
     7681 tatgctcatc gtctaaagaa ctacccattt tattatatat tagtcacgat atctataaca
     7741 agaaaatata tatataataa gttatcacgt aagtagaaca tgaaataaca atataattat
     7801 cgtatgagtt aaatcttaaa agtcacgtaa aagataatca tgcgtcattt tgactcacgc
     7861 ggtcgttata gttcaaaatc agtgacactt accgcattga caagcacgcc tcacgggagc
     7921 tccaagcggc gactgagatg tcctaaatgc acagcgacgg attcgcgcta tttagaaaga
     7981 gagagcaata tttcaagaat gcatgcgtca attttacgca gactatcttt ctagggttaa
     8041 aaaagatttg cgctttactc gacctaaact ttaaacacgt catagaatct tcgtttgaca
     8101 aaaaccacat tgtggccaag ctgtgtgacg cgacgcgcgc taaagaatgg caaaccaagt
     8161 cgcgcgaggt accagctttt gttcccttta gtgagggtta atttcgagct tggcgtaatc
     8221 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     8281 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     8341 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     8401 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     8461 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     8521 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     8581 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     8641 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     8701 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     8761 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     8821 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8881 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8941 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     9001 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     9061 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     9121 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     9181 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     9241 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     9301 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     9361 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     9421 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     9481 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     9541 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     9601 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     9661 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     9721 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     9781 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9841 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9901 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9961 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    10021 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    10081 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    10141 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    10201 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    10261 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    10321 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacct
//
