LOCUS       sequence-474320-        8353 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression construct for OsTir1, Hyg resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8353)
  TITLE     Hendrich lab plasmids batch1
REFERENCE   2  (bases 1 to 8353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PB CAG Tir1 iHyg
FEATURES             Location/Qualifiers
     source          1..8353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(35..52)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(206..794)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(286..305)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(965..1756,1757..1825))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1588..1607
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1826..1930)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1956..2411)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2093..2114)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2305..2324
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2538..2560
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2552..2569
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2553..2569
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2576..2595
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2576..2594
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   2613..2647
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    2922..2955
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        2965..3344
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     3608..3630
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3692..3711
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    3748..3772
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3780..3789
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3801..3821
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3828..5552
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="TYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFVG
                     NCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEEL
                     RMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEVE
                     DRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTLA
                     KILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYPL
                     CAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELRV
                     FPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRLC
                     ILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSIA
                     FAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKGC
                     QVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     protein_bind    complement(5553..5577)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(5729..6303,6304..6306,6307..6315)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5896..5913)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6123..6142
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6316..7341
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVHRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(7644..7660)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7668..7684
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7692..7698,7699..7716,7717..7722))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7737..7758
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    7828..7861
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     repeat_region   8032..8094
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(8118..8136)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8157..8173)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8181..8197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8205..8211,8212..8229,8230..8235))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8250..8271
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
       61 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
      121 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
      181 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      241 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      301 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      361 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      421 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      481 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      541 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      601 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
      661 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      721 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      781 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      841 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
      901 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
      961 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     1021 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1081 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     1141 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1201 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1261 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1321 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1381 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     1441 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     1501 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     1561 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     1621 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     1681 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     1741 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     1801 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     1861 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     1921 gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt
     1981 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     2041 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     2101 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     2161 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     2221 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     2281 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     2341 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     2401 acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     2461 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     2521 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata
     2581 cgactcacta tagggcgaat tgggtacctt aaccctagaa agatagtctg cgtaaaattg
     2641 acgcatgcat tcttgaaata ttgctctctc tttctaaata gcgcgaatcc gtcgctgtgc
     2701 atttaggaca tctcagtcgc cgcttggagc tcccgtgagg cgtgcttgtc aatgcggtaa
     2761 gtgtcactga ttttgaacta taacgaccgc gtgagtcaaa atgacgcatg attatctttt
     2821 acgtgacttt taagatttaa ctcatacgat aattatattg ttatttcatg ttctacttac
     2881 gtgataactt attatatata tattttcttg ttatagatat cataacttcg tataatgtat
     2941 gctatacgaa gttatgcggc cctcgacatt gattattgac tagttattaa tagtaatcaa
     3001 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     3061 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     3121 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     3181 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     3241 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     3301 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
     3361 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
     3421 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggcgc gcgccaggcg
     3481 gggcggggcg gggcgagggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt
     3541 ctccctctcc agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc
     3601 agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt
     3661 catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat
     3721 cattttggca aagaattcct cgaatcaaca agtttgtaca aaaaagcagg ctacccgccg
     3781 ccaccatggg ccccgccgcc cccaagaaga agagaaaggt gctggacacg tacttccctg
     3841 aagaagttgt ggaacatatc ttttcttttc ttcctgccca aagagaccgg aatacagtct
     3901 ctttggtatg taaggtttgg tatgagattg aaaggttgtc aaggagagga gtgtttgtgg
     3961 gaaactgcta tgcggtaagg gctggaagag tcgctgccag attccccaat gtgagggcac
     4021 ttacagttaa gggtaaacca cattttgctg atttcaattt ggttcctccg gactggggag
     4081 gttatgctgg accatggatt gaggctgctg ccagaggttg tcatgggttg gaggagttga
     4141 ggatgaagag gatggttgtg tctgatgagt cccttgaact tctggcccgg tccttcccca
     4201 gattcagagc tttggtcttg atttcttgtg agggtttctc tactgacgga ctcgctgcag
     4261 tcgcttccca ctgtaagttg ttgagggagt tggacctcca agaaaatgag gttgaggaca
     4321 gaggaccaag atggttgtca tgtttccctg actcatgtac ttcccttgtt tccttgaatt
     4381 tcgcgtgtat caaaggagag gtcaatgccg ggagtcttga gagattggtc tcaagaagtc
     4441 ccaacttgcg atcactgagg ctcaacagat ctgtgagtgt ggataccttg gcaaagattc
     4501 tcttgagaac ccccaatttg gaggaccttg gaactgggaa tctcacggat gacttccaaa
     4561 ctgagtctta ctttaagttg actagtgctt tggaaaagtg taagatgttg agatcactgt
     4621 ccggattctg ggatgcttca cctgtttgtc tttccttcat ctaccctctt tgtgcccagc
     4681 tgactggact caacttgtcc tatgctccaa cactggatgc gagtgacttg accaagatga
     4741 tttcaagatg cgtgaagctt caaagacttt gggtcctcga ttgtatctcc gacaaaggac
     4801 ttcaagtggt ggcatcatct tgtaaggatc tccaagagtt gagagtgttt ccaagtgact
     4861 tctatgtggc aggttacagt gctgtaactg aggaagggtt ggttgctgtc tcccttggat
     4921 gtcccaagtt gaattcactc ttgtactttt gtcaccaaat gactaacgct gcccttgtga
     4981 cagtggccaa aaattgccct aacttcacac gcttcagact ttgtatcctt gaacctggga
     5041 aacctgacgt ggtcaccagt cagcccttgg acgaaggatt tggggcgatt gtgagagaat
     5101 gtaagggact ccaaagactc tctatcagtg gactcctgac tgacaaggtg tttatgtaca
     5161 ttggtaagta cgctaagcag ctggaaatgc tgtctattgc ctttgccggt gattctgaca
     5221 aggggatgat gcatgttatg aatggatgta agaacttgag aaagcttgag ataagagaca
     5281 gtccattcgg agatgcagcc ctccttggta acttcgcaag atacgaaact atgaggtctc
     5341 tttggatgtc cagctgtaat gttacactca agggatgtca agtgctggcc tcaaaaatgc
     5401 caatgctgaa tgtggaggtg atcaacgaga gagatggatc caatgaaatg gaagagaacc
     5461 acggagatct tcccaaggtg gaaaagttgt atgtctacag aaccactgct ggggcaaggg
     5521 atgacgctcc aaacttcgtg aaaatactgt gaacccagct ttcttgtaca aagtggttga
     5581 ttcgaggccg ctcgataagc ttgatatcga attcactcct caggtgcagg ctgcctatca
     5641 gaaggtggtg gctggtgtgg ccaactggct cacaaatacc actgagatcg acggtatcga
     5701 taagcttgat atcgaattcc gccccccccc cctctccctc ccccccccct aacgttactg
     5761 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
     5821 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc
     5881 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag
     5941 cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc
     6001 ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac
     6061 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca
     6121 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt
     6181 gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa
     6241 aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat
     6301 aatatggcca caaccatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg
     6361 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
     6421 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
     6481 ggtttctaca aagatcgtta tgttcatcgg cactttgcat cggccgcgct cccgattccg
     6541 gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca
     6601 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
     6661 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
     6721 ttcggaccgc aaggaatcgg tcaatacact acgtggcgtg atttcatatg cgcgattgct
     6781 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
     6841 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
     6901 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
     6961 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     7021 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
     7081 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
     7141 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
     7201 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
     7261 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
     7321 cgtccgaggg caaaggaata gtcgagaaat tgatgatcta ttaagcaata aagacgtcca
     7381 ctaaaatgga agtttttcct gtcatacttt gttaagaagg gtgagaacag agtacctaca
     7441 ttttgaatgg aaggattgga gctacggggg tgggggtggg gtgggattag ataaatgcct
     7501 gctctttact gaaggctctt tactattgct ttatgataat gtttcatagt tggatatcat
     7561 aatttaaaca agcaaaacca aattaagggc cagctcattc ctccactcac gatctataga
     7621 tccactagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     7681 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     7741 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     7801 tcgtgccagc ggatcggccg cgtcgagata acttcgtata atgtatgcta tacgaagtta
     7861 tgttttgtta ctttatagaa gaaattttga gtttttgttt ttttttaata aataaataaa
     7921 cataaataaa ttgtttgttg aatttattat tagtatgtaa gtgtaaatat aataaaactt
     7981 aatatctatt caaattaata aataaacctc gatatacaga ccgataaaac acatgcgtca
     8041 attttacgca tgattatctt taacgtacgt cacaatatga ttatctttct agggttaaag
     8101 agctccagct tttgttccct ttagtgaggg ttaatttcga gcttggcgta atcatggtca
     8161 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     8221 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     8281 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     8341 caacgcgcgg gga
//
