LOCUS       sequence-474326-        9220 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector that can express doxycycline-inducible hAqp1 with 
            fkbp12 degron in Tet ON mammalian cells. CMVtight promoter. EGFP 
            marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9220)
  AUTHORS   Yun J, Huang Y, Miller ADC, Chang BL, Baldini L, Dhanabalan KM, Li 
            E, Li H, Mukherjee A
  TITLE     Destabilized reporters for background-subtracted, chemically-gated, 
            and multiplexed deep-tissue imaging.
  JOURNAL   Chem Sci. 2024 Jun 4;15(28):11108-11121. doi: 10.1039/d4sc00377b. 
            eCollection 2024 Jul 17.
  PUBMED    39027298
REFERENCE   2  (bases 1 to 9220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/d4sc00377b"; journalName: "Chem Sci"; date: "2024-06-4- 4";
            volume: "15"; issue: "28"; pages: "11108-11121"
COMMENT     Sequence Label: pL(CMVtight-phi-AQP1-FKBP12DD-IRES-EGFP-WPRE)
FEATURES             Location/Qualifiers
     source          1..9220
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2859..2976
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2998..3312
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    3006..3024
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3042..3060
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3078..3096
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3113..3131
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3149..3167
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3184..3202
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3220..3238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3285..3309
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3390..3414
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3443..3495
                     /label=MCS
                     /note="multiple cloning site"
     CDS             3537..3560
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4365..4685
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KPE"
     misc_feature    join(4689..5263,5264..5266,5267..5275)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4856..4873)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5083..5102
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5276..5278,5279..5281,5282..5995)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5276..5278,5279..5281,5282..5992)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5321..5342)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5582..5601)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5929..5950
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(6020..6044)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6075..6663
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6128..6148)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6546..6557)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6796..7029
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7101..7222
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7138..7157)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7192..7211
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7262..7397
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7417..7436)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7418..7436)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7446..7463)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7446..7462)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7455..7477)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7604..8059
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7691..7710)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7901..7922
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8085..8189
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8190..8258,8259..9050)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8408..8427)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcga gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt
     2881 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2941 gaattacaaa aacaaattac aaaaattcaa aattttatcg atcacgagac tagcctcgag
     3001 tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga
     3061 acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt tatccctatc
     3121 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt
     3181 ttatccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
     3241 gtatgtcgag gtaggcgtgt acggtgggag gcctatataa gcagagctcg tttagtgaac
     3301 cgtcagatcg cctggagaat tcgagctcgg tacccgggga tccaccggtt cgtcgactag
     3361 tccagtgtgg tggaattctg cagatatcaa caagtttgta caaaaaagca ggctttaaag
     3421 gaaccaattc agtcgactgg atctcgagct caagcttcga attctgcagt cgacggtacc
     3481 gcgggcccgg gatccaccgg tcgccaccga gctcggatcc agtacccttc accatggact
     3541 acaaggacga cgacgacaag gccagcgagt tcaagaagaa gctcttctgg agggcagtgg
     3601 tggccgagtt cctggccacg accctctttg tcttcatcag catcggttct gccctgggct
     3661 tcaaataccc ggtggggaac aaccagacgg cggtccagga caacgtgaag gtgtcgctgg
     3721 ccttcgggct gagcatcgcc acgctggcgc agagtgtggg ccacatcagc ggcgcccacc
     3781 tcaacccggc tgtcacactg gggctgctgc tcagctgcca gatcagcatc ttccgtgccc
     3841 tcatgtacat catcgcccag tgcgtggggg ccatcgtcgc caccgccatc ctctcaggca
     3901 tcacctcctc cctgactggg aactcgcttg gccgcaatga cctggctgat ggtgtgaact
     3961 cgggccaggg cctgggcatc gagatcatcg ggaccctcca gctggtgcta tgcgtgctgg
     4021 ctactaccga ccggaggcgc cgtgaccttg gtggctcagc cccccttgcc atcggcctct
     4081 ctgtagccct tggacacctc ctggctattg actacactgg ctgtgggatt aaccctgctc
     4141 ggtcctttgg ctccgcggtg atcacacaca acttcagcaa ccactggatt ttctgggtgg
     4201 ggccattcat cgggggagcc ctggctgtac tcatctacga cttcatcctg gccccacgca
     4261 gcagtgacct cacagaccgc gtgaaggtgt ggaccagcgg ccaggtggag gagtatgacc
     4321 tggatgccga cgacatcaac tccagggtgg agatgaagcc caaaggagtg caggtggaaa
     4381 ccatctcccc aggagacggg cgcaccttcc ccaagcgcgg ccagacctgt gtggtgcact
     4441 acaccgggat gcttgaagat ggaaagaaag tcgattcctc ccgggacaga aacaagccct
     4501 ttaagtttat gctaggcaag caggaggtga tccgaggctg ggaagaaggg gttgcccaga
     4561 tgagtgtggg tcagagagcc aaactgacta tatctccaga ttatgcctat ggtgccactg
     4621 ggcacccagg catcatccca ccacatgcca ctctcgtctt cgatgtggag cttctaaaac
     4681 cggaatagcc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa
     4741 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     4801 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     4861 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     4921 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     4981 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     5041 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     5101 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     5161 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     5221 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatggt
     5281 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     5341 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     5401 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     5461 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     5521 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     5581 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     5641 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     5701 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     5761 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     5821 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     5881 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     5941 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcgg
     6001 ccgcactcga gatatctaga cccagctttc ttgtacaaag tggttgatat ccagcacagt
     6061 ggcggccgct cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     6121 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     6181 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     6241 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     6301 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     6361 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     6421 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     6481 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     6541 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     6601 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     6661 cgcctggaat tctgcagata tccggactag tgatctaatt ctaccgggga tccgtcgagg
     6721 gcccggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     6781 aagaaaaggg gggactggaa gggctagctc actcccaacg aagacaagat ctgctttttg
     6841 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     6901 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     6961 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     7021 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     7081 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     7141 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     7201 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     7261 catcccgccc ctaactcagc ccagttccgc ccattctccg ccccatggct gactaatttt
     7321 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     7381 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     7441 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     7501 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     7561 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     7621 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     7681 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     7741 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     7801 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     7861 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     7921 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     7981 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     8041 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     8101 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     8161 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     8221 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     8281 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     8341 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     8401 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     8461 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     8521 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     8581 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     8641 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     8701 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     8761 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     8821 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     8881 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     8941 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     9001 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     9061 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     9121 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     9181 actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
