LOCUS       sequence-474327-        9888 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector that can express hAqp1 with fkbp12 degron in 
            mammalian cells. EF1a promoter. EGFP marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9888)
  AUTHORS   Yun J, Huang Y, Miller ADC, Chang BL, Baldini L, Dhanabalan KM, Li 
            E, Li H, Mukherjee A
  TITLE     Destabilized reporters for background-subtracted, chemically-gated, 
            and multiplexed deep-tissue imaging.
  JOURNAL   Chem Sci. 2024 Jun 4;15(28):11108-11121. doi: 10.1039/d4sc00377b. 
            eCollection 2024 Jul 17.
  PUBMED    39027298
REFERENCE   2  (bases 1 to 9888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/d4sc00377b"; journalName: "Chem Sci"; date: "2024-06-4- 4";
            volume: "15"; issue: "28"; pages: "11108-11121"
COMMENT     Sequence Label: pL(EF1a-phi-AQP1-FKBP12DD-IRES-EGFP-WPRE)
FEATURES             Location/Qualifiers
     source          1..9888
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2859..2976
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2998..4176
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3229..4167
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4124..4144
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4205..4228
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5033..5353
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KPE"
     misc_feature    join(5357..5931,5932..5934,5935..5943)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5524..5541)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5751..5770
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5944..5946,5947..5949,5950..6663)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5944..5946,5947..5949,5950..6660)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5989..6010)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6250..6269)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6597..6618
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(6688..6712)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6743..7331
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6796..6816)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7214..7225)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7464..7697
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7769..7890
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7806..7825)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7860..7879
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7930..8065
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8085..8104)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8086..8104)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8114..8131)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8114..8130)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8123..8145)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8272..8727
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8359..8378)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8569..8590
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8753..8857
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8858..8926,8927..9718)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9076..9095)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctggga catcaagcag ctccaggcga gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt
     2881 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2941 gaattacaaa aacaaattac aaaaattcaa aattttatcg atcacgagac tagcctcggc
     3001 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga
     3061 ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
     3121 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
     3181 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg
     3241 tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact
     3301 tccacctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag
     3361 agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct
     3421 gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc
     3481 gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa
     3541 gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg
     3601 ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg
     3661 cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc
     3721 ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt
     3781 gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg
     3841 cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc
     3901 tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag
     3961 ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag
     4021 tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc
     4081 tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg
     4141 gttcaaagtt tttttcttcc atttcaggtg tcgtgagagc tcggatccag tacccttcac
     4201 catggactac aaggacgacg acgacaaggc cagcgagttc aagaagaagc tcttctggag
     4261 ggcagtggtg gccgagttcc tggccacgac cctctttgtc ttcatcagca tcggttctgc
     4321 cctgggcttc aaatacccgg tggggaacaa ccagacggcg gtccaggaca acgtgaaggt
     4381 gtcgctggcc ttcgggctga gcatcgccac gctggcgcag agtgtgggcc acatcagcgg
     4441 cgcccacctc aacccggctg tcacactggg gctgctgctc agctgccaga tcagcatctt
     4501 ccgtgccctc atgtacatca tcgcccagtg cgtgggggcc atcgtcgcca ccgccatcct
     4561 ctcaggcatc acctcctccc tgactgggaa ctcgcttggc cgcaatgacc tggctgatgg
     4621 tgtgaactcg ggccagggcc tgggcatcga gatcatcggg accctccagc tggtgctatg
     4681 cgtgctggct actaccgacc ggaggcgccg tgaccttggt ggctcagccc cccttgccat
     4741 cggcctctct gtagcccttg gacacctcct ggctattgac tacactggct gtgggattaa
     4801 ccctgctcgg tcctttggct ccgcggtgat cacacacaac ttcagcaacc actggatttt
     4861 ctgggtgggg ccattcatcg ggggagccct ggctgtactc atctacgact tcatcctggc
     4921 cccacgcagc agtgacctca cagaccgcgt gaaggtgtgg accagcggcc aggtggagga
     4981 gtatgacctg gatgccgacg acatcaactc cagggtggag atgaagccca aaggagtgca
     5041 ggtggaaacc atctccccag gagacgggcg caccttcccc aagcgcggcc agacctgtgt
     5101 ggtgcactac accgggatgc ttgaagatgg aaagaaagtc gattcctccc gggacagaaa
     5161 caagcccttt aagtttatgc taggcaagca ggaggtgatc cgaggctggg aagaaggggt
     5221 tgcccagatg agtgtgggtc agagagccaa actgactata tctccagatt atgcctatgg
     5281 tgccactggg cacccaggca tcatcccacc acatgccact ctcgtcttcg atgtggagct
     5341 tctaaaaccg gaatagcccc tctccctccc ccccccctaa cgttactggc cgaagccgct
     5401 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
     5461 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     5521 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     5581 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     5641 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     5701 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
     5761 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     5821 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag
     5881 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca
     5941 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     6001 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     6061 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     6121 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     6181 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     6241 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     6301 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     6361 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     6421 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     6481 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     6541 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     6601 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     6661 taaagcggcc gcactcgaga tatctagacc cagctttctt gtacaaagtg gttgatatcc
     6721 agcacagtgg cggccgctcg acaatcaacc tctggattac aaaatttgtg aaagattgac
     6781 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     6841 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     6901 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     6961 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     7021 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     7081 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     7141 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     7201 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     7261 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     7321 cgcctccccg cctggaattc tgcagatatc cggactagtg atctaattct accggggatc
     7381 cgtcgagggc ccggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     7441 ctttttaaaa gaaaaggggg gactggaagg gctagctcac tcccaacgaa gacaagatct
     7501 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7561 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     7621 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     7681 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     7741 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     7801 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     7861 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     7921 actccgccca tcccgcccct aactcagccc agttccgccc attctccgcc ccatggctga
     7981 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     8041 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt
     8101 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     8161 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     8221 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
     8281 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     8341 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     8401 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     8461 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     8521 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     8581 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     8641 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     8701 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     8761 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     8821 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     8881 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     8941 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     9001 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     9061 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     9121 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     9181 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     9241 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     9301 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     9361 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     9421 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     9481 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     9541 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     9601 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     9661 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     9721 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     9781 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     9841 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttc
//
