LOCUS       sequence-474328-       11134 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Puromycin selected lentiviral construct with cumate-inducible 
            PAX3::FOXO1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11134)
  AUTHORS   Kent MR, Jay AN, Kendall GC
  TITLE     New dual inducible cellular model to investigate temporal control of
            oncogenic cooperating genes.
  JOURNAL   Sci Rep. 2024 Sep 5;14(1):20773. doi: 10.1038/s41598-024-71227-3.
  PUBMED    39237585
REFERENCE   2  (bases 1 to 11134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2024-09-5"; volume: "14(1):20773. doi"; pages: " 
            10.1038/s41598-024-71227-3"
COMMENT     Sequence Label: pLV-Puro-{CMV-CuO}>{PAX3::FOXO1}:T2A:mCherry
FEATURES             Location/Qualifiers
     source          1..11134
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        1959..2262
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2263..2466
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2417..2437
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2467..2494
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2494..2518
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5042..5095
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5096..5806
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5241..5259)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5509..5528
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(5807..5831)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5849..6437
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5902..5922)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6320..6331)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6468..6967
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6503..6526)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6900..6919
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6984..7583
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6984..7003)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7480..7500
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7655..7888
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7960..8081
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7997..8016)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8051..8070
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8121..8256
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8276..8295)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8277..8295)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8305..8322)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8305..8321)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8314..8336)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8463..8918
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8550..8569)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8760..8781
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8944..9048
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9049..9117,9118..9909)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9267..9286)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10080..10668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10569..10588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10822..10839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10956..10977
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10992..10997,10998..11015,11016..11022)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11030..11046
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11035..11057
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11054..11070
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11054..11070
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11089..11109
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11091..11109
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgcg ttacataact tacggtaaat
     1981 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     2041 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     2101 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     2161 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     2221 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     2281 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     2341 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     2401 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     2461 agagctaaca aacagacaat ctggtctgtt tgtacaagtt tgtacaaaaa agcaggctgc
     2521 caccatgacc acgctggccg gcgctgtgcc caggatgatg cggccgggcc cggggcagaa
     2581 ctacccgcgt agcgggttcc cgctggaagt gtccactccc ctcggccagg gccgcgtcaa
     2641 ccagctcggc ggcgttttta tcaacggcag gccgctgccc aaccacatcc gccacaagat
     2701 cgtggagatg gcccaccacg gcatccggcc ctgcgtcatc tcgcgccagc tgcgcgtgtc
     2761 ccacggctgc gtctccaaga tcctgtgcag gtaccaggag actggctcca tacgtcctgg
     2821 tgccatcggc ggcagcaagc ccaagcaggt gacaacgcct gacgtggaga agaaaattga
     2881 ggaatacaaa agagagaacc cgggcatgtt cagctgggaa atccgagaca aattactcaa
     2941 ggacgcggtc tgtgatcgaa acaccgtgcc gtcagtgagt tccatcagcc gcatcctgag
     3001 aagtaaattc gggaaaggtg aagaggagga ggccgacttg gagaggaagg aggcagagga
     3061 aagcgagaag aaggccaaac acagcatcga cggcatcctg agcgagcgag cctcagcacc
     3121 ccaatcagat gaaggctctg atattgactc tgaaccagat ttaccactaa agaggaaaca
     3181 gcgcagaagc cgaaccacct tcacagcaga acagctggag gaactggagc gtgcttttga
     3241 gagaactcat taccctgaca tttatactag ggaggaactg gcccagaggg cgaagctcac
     3301 cgaggcccga gtacaggtct ggtttagcaa ccgccgtgca agatggagga agcaagctgg
     3361 ggccaatcaa ctgatggctt tcaaccatct cattcccggg gggttccctc ccactgccat
     3421 gccgaccttg ccaacgtacc agctgtcgga gacctcttac cagcccacat ctattccaca
     3481 agctgtgtca gatcccagca gcaccgttca cagacctcaa ccgcttcctc caagcactgt
     3541 acaccaaagc acgattcctt ccaacccaga cagcagctct gcctactgcc tccccagcac
     3601 caggcatgga ttttccagct atacagacag ctttgtgcct ccgtcggggc cctccaaccc
     3661 catgaacccc accattggca atggcctctc acctcagaat tcaattcgtc ataatctgtc
     3721 cctacacagc aagttcattc gtgtgcagaa tgaaggaact ggaaaaagtt cttggtggat
     3781 gctcaatcca gagggtggca agagcgggaa atctcctagg agaagagctg catccatgga
     3841 caacaacagt aaatttgcta agagccgaag ccgagctgcc aagaagaaag catctctcca
     3901 gtctggccag gagggtgctg gggacagccc tggatcacag ttttccaaat ggcctgcaag
     3961 ccctggctct cacagcaatg atgactttga taactggagt acatttcgcc ctcgaactag
     4021 ctcaaatgct agtactatta gtgggagact ctcacccatt atgaccgaac aggatgatct
     4081 tggagaaggg gatgtgcatt ctatggtgta cccgccatct gccgcaaaga tggcctctac
     4141 tttacccagt ctgtctgaga taagcaatcc cgaaaacatg gaaaatcttt tggataatct
     4201 caaccttctc tcatcaccaa catcattaac tgtttcgacc cagtcctcac ctggcaccat
     4261 gatgcagcag acgccgtgct actcgtttgc gccaccaaac accagtttga attcacccag
     4321 cccaaactac caaaaatata catatggcca atccagcatg agccctttgc cccagatgcc
     4381 tatacaaaca cttcaggaca ataagtcgag ttatggaggt atgagtcagt ataactgtgc
     4441 gcctggactc ttgaaggagt tgctgacttc tgactctcct ccccataatg acattatgac
     4501 accagttgat cctggggtag cccagcccaa cagccgggtt ctgggccaga acgtcatgat
     4561 gggccctaat tcggtcatgt caacctatgg cagccaggca tctcataaca aaatgatgaa
     4621 tcccagctcc catacccacc ctggacatgc tcagcagaca tctgcagtta acgggcgtcc
     4681 cctgccccac acggtaagca ccatgcccca cacctcgggt atgaaccgcc tgacccaagt
     4741 gaagacacct gtacaagtgc ctctgcccca ccccatgcag atgagtgccc tggggggcta
     4801 ctcctccgtg agcagctgca atggctatgg cagaatgggc cttctccacc aggagaagct
     4861 cccaagtgac ttggatggca tgttcattga gcgcttagac tgtgacatgg aatccatcat
     4921 tcggaatgac ctcatggatg gagatacatt ggattttaac tttgacaatg tgttgcccaa
     4981 ccaaagcttc ccacacagtg tcaagacaac gacacatagc tgggtgtcag gcggaagcgg
     5041 agagggcagg ggaagtcttc taacatgcgg ggacgtggag gaaaatcccg gccccatggt
     5101 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
     5161 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     5221 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     5281 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     5341 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     5401 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     5461 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     5521 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     5581 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     5641 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     5701 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     5761 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaaaccc agctttcttg
     5821 tacaaagtgg tgataatcga attccgataa tcaacctctg gattacaaaa tttgtgaaag
     5881 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     5941 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     6001 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     6061 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     6121 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     6181 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     6241 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     6301 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     6361 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     6421 ttgggccgcc tccccgcatc gggaattccc gcggttcgaa ttctaccggg taggggaggc
     6481 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
     6541 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
     6601 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
     6661 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
     6721 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
     6781 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
     6841 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
     6901 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
     6961 cctttcgacc tcacgtggcc accatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     7021 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     7081 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     7141 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     7201 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     7261 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     7321 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     7381 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     7441 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     7501 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     7561 tgacccgcaa gcccggtgcc tgaggtacct ttaagaccaa tgacttacaa ggcagctgta
     7621 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     7681 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     7741 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     7801 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     7861 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     7921 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     7981 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     8041 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     8101 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     8161 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     8221 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
     8281 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     8341 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     8401 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     8461 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     8521 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     8581 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     8641 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     8701 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     8761 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     8821 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     8881 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     8941 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     9001 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     9061 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     9121 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     9181 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     9241 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     9301 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     9361 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     9421 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     9481 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     9541 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     9601 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     9661 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     9721 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     9781 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     9841 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     9901 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     9961 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
    10021 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
    10081 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
    10141 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
    10201 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
    10261 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
    10321 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
    10381 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
    10441 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaagag
    10501 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
    10561 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
    10621 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    10681 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
    10741 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
    10801 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
    10861 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
    10921 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
    10981 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
    11041 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
    11101 cactaaaggg aacaaaagct ggagctgcaa gctt
//
