LOCUS       sequence-474335-       11056 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A version of the episome pPtGE31 with a deleted nonessential region,
            contains the NAT cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11056)
  TITLE     Spheroplasted cells: a game changer for DNA delivery to diatoms
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pPtGE31_Delta-PtR
FEATURES             Location/Qualifiers
     source          1..11056
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      66..680
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      157..688
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      756..975
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             1066..1821
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    1824..2074
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     misc_feature    2236..2739
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        2740..2926
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             2927..3589
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     CDS             3779..4954
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    6843..7241
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     protein_bind    complement(7259..7292)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     7360..7379
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(7459..7734)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSYLGETIEYSSLHACRSTLEDPRVPSSNSPYSESYYNSL
                     AVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS"
     primer_bind     7572..7594
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7586..7603
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7587..7603
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7610..7629
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7610..7628
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    7631..7687
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(7730..7746)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7730..7746)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7743..7765)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7754..7770
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7778..7784,7785..7802,7803..7808))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7823..7844
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(8064..8723)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     8638..8657
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(8724..8826)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             10279..10848
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGFT
                     LREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNRR
                     LTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFTL
                     CGLDTALYDGTASDGEQALYMSMPCP"
ORIGIN
        1 agtctctatc cttccttaaa aatttaattt tcattagttg cagtcactcc gctttggttt
       61 tcgagctggt tgccctcgcc gctgggctgg cggccgtcta tggccctgca aacgcgccag
      121 aaacgccgtc gaagccgtgt gcgagacacc gcggccggcc gccggcgttg tggatacctc
      181 gcggaaaact tggccctcac tgacagatga ggggcggacg ttgacacttg aggggccgac
      241 tcacccggcg cggcgttgac agatgagggg caggctcgat ttcggccggc gacgtggagc
      301 tggccagcct cgcaaatcgg cgaaaacgcc tgattttacg cgagtttccc acagatgatg
      361 tggacaagcc tggggataag tgccctgcgg tattgacact tgaggggcgc gactactgac
      421 agatgagggg cgcgatcctt gacacttgag gggcagagtg ctgacagatg aggggcgcac
      481 ctattgacat ttgaggggct gtccacaggc agaaaatcca gcatttgcaa gggtttccgc
      541 ccgtttttcg gccaccgcta acctgtcttt taacctgctt ttaaaccaat atttataaac
      601 cttgttttta accagggctg cgccctgtgc gcgtgaccgc gcacgccgaa ggggggtgcc
      661 cccccttctc gaaccctccc ggtcgagtga gcgaggaagc accagggaac agcacttata
      721 tattctgctt acacacgatg cctgaaaaaa cttcccttgg ggttatccac ttatccacgg
      781 ggatattttt ataattattt tttttatagt ttttagatct tcttttttag agcgccttgt
      841 aggcctttat ccatgctggt tctagagaag gtgttgtgac aaattgccct ttcagtgtga
      901 caaatcaccc tcaaatgaca gtcctgtctg tgacaaattg cccttaaccc tgtgacaaat
      961 tgccctcaga agaagctgtt ttttcacaaa gttatccctg cttattgact cttttttatt
     1021 tagtgtgaca atctaaaaac ttgtcacact tcacatggat ctgtcatggc ggaaacagcg
     1081 gttatcaatc acaagaaacg taaaaatagc ccgcgaatcg tccagtcaaa cgacctcact
     1141 gaggcggcat atagtctctc ccgggatcaa aaacgtatgc tgtatctgtt cgttgaccag
     1201 atcagaaaat ctgatggcac cctacaggaa catgacggta tctgcgagat ccatgttgct
     1261 aaatatgctg aaatattcgg attgacctct gcggaagcca gtaaggatat acggcaggca
     1321 ttgaagagtt tcgcggggaa ggaagtggtt ttttatcgcc ctgaagagga tgccggcgat
     1381 gaaaaaggct atgaatcttt tccttggttt atcaaacgtg cgcacagtcc atccagaggg
     1441 ctttacagtg tacatatcaa cccatatctc attcccttct ttatcgggtt acagaaccgg
     1501 tttacgcagt ttcggcttag tgaaacaaaa gaaatcacca atccgtatgc catgcgttta
     1561 tacgaatccc tgtgtcagta tcgtaagccg gatggctcag gcatcgtctc tctgaaaatc
     1621 gactggatca tagagcgtta ccagctgcct caaagttacc agcgtatgcc tgacttccgc
     1681 cgccgcttcc tgcaggtctg tgttaatgag atcaacagca gaactccaat gcgcctctca
     1741 tacattgaga aaaagaaagg ccgccagacg actcatatcg tattttcctt ccgcgatatc
     1801 acttccatga cgacaggata gtctgagggt tatctgtcac agatttgagg gtggttcgtc
     1861 acatttgttc tgacctactg agggtaattt gtcacagttt tgctgtttcc ttcagcctgc
     1921 atggattttc tcatactttt tgaactgtaa tttttaagga agccaaattt gagggcagtt
     1981 tgtcacagtt gatttccttc tctttccctt cgtcatgtga cctgatatcg ggggttagtt
     2041 cgtcatcatt gatgagggtt gattatcaca gtttattact ctgaattggc tatccgcgtg
     2101 tgtacctcta cctggagttt ttcccacggt ggatatttct tcttgcgctg agcgtaagag
     2161 ctatctgaca gaacagttct tctttgcttc ctcgccagtt cgctcgctat gctcggttac
     2221 acggctgcgg cgagcatcac gtgctataaa aataattata atttaaattt tttaatataa
     2281 atatataaat taaaaataga aagtaaaaaa agaaattaaa gaaaaaatag tttttgtttt
     2341 ccgaagatgt aaaagactct agggggatcg ccaacaaata ctacctttta ccttgctctt
     2401 cctgctctca ggtattaatg ccgaattgtt tcatcttgtc tgtgtagaag accacacacg
     2461 aaaatcctgt gattttacat tttacttatc gttaatcgaa tgtatatcta tttaatctgc
     2521 ttttcttgtc taataaatat atatgtaaag tacgcttttt gttgaaattt tttaaacctt
     2581 tgtttatttt tttttcttca ttccgtaact cttctacctt ctttatttac tttctaaaat
     2641 ccaaatacaa aacataaaaa taaataaaca cagagtaaat tcccaaatta ttccatcatt
     2701 aaaagatacg aggcgcgtgt aagttacagg caagcgatcc tagtacactc tatatttttt
     2761 tatgcctcgg taatgatttt catttttttt ttccacctag cggatgactc tttttttttc
     2821 ttagcgattg gcattatcac ataatgaatt atacattata taaagtaatg tgatttcttc
     2881 gaagaatata ctaaaaaatg agcaggcaag ataaacgaag gcaaagatga cagagcagaa
     2941 agccctagta aagcgtatta caaatgaaac caagattcag attgcgatct ctttaaaggg
     3001 tggtccccta gcgatagagc actcgatctt cccagaaaaa gaggcagaag cagtagcaga
     3061 acaggccaca caatcgcaag tgattaacgt ccacacaggt atagggtttc tggaccatat
     3121 gatacatgct ctggccaagc attccggctg gtcgctaatc gttgagtgca ttggtgactt
     3181 acacatagac gaccatcaca ccactgaaga ctgcgggatt gctctcggtc aagcttttaa
     3241 agaggcccta ggggccgtgc gtggagtaaa aaggtttgga tcaggatttg cgcctttgga
     3301 tgaggcactt tccagagcgg tggtagatct ttcgaacagg ccgtacgcag ttgtcgaact
     3361 tggtttgcaa agggagaaag taggagatct ctcttgcgag atgatcccgc attttcttga
     3421 aagctttgca gaggctagca gaattaccct ccacgttgat tgtctgcgag gcaagaatga
     3481 tcatcaccgt agtgagagtg cgttcaaggc tcttgcggtt gccataagag aagccacctc
     3541 gcccaatggt accaacgatg ttccctccac caaaggtgtt cttatgtagt tttacacagg
     3601 agtctggact tgacgctagt gataataagt gactgaggta tgtgctcttc ttatctcctt
     3661 ttgtagtgtt gctcttattt taaacaactt tgcggttttt tgatgacttt gcgattttgt
     3721 tgttgctttg cagtaaattg caagatttaa taaaaaaacg caaagcaatg attaaaggat
     3781 gttcagaatg aaactcatgg aaacacttaa ccagtgcata aacgctggtc atgaaatgac
     3841 gaaggctatc gccattgcac agtttaatga tgacagcccg gaagcgagga aaataacccg
     3901 gcgctggaga ataggtgaag cagcggattt agttggggtt tcttctcagg ctatcagaga
     3961 tgccgagaaa gcagggcgac taccgcaccc ggatatggaa attcgaggac gggttgagca
     4021 acgtgttggt tatacaattg aacaaattaa tcatatgcgt gatgtgtttg gtacgcgatt
     4081 gcgacgtgct gaagacgtat ttccaccggt gatcggggtt gctgcccata aaggtggcgt
     4141 ttacaaaacc tcagtttctg ttcatcttgc tcaggatctg gctctgaagg ggctacgtgt
     4201 tttgctcgtg gaaggtaacg acccccaggg aacagcctca atgtatcacg gatgggtacc
     4261 agatcttcat attcatgcag aagacactct cctgcctttc tatcttgggg aaaaggacga
     4321 tgtcacttat gcaataaagc ccacttgctg gccggggctt gacattattc cttcctgtct
     4381 ggctctgcac cgtattgaaa ctgagttaat gggcaaattt gatgaaggta aactgcccac
     4441 cgatccacac ctgatgctcc gactggccat tgaaactgtt gctcatgact atgatgtcat
     4501 agttattgac agcgcgccta acctgggtat cggcacgatt aatgtcgtat gtgctgctga
     4561 tgtgctgatt gttcccacgc ctgctgagtt gtttgactac acctccgcac tgcagttttt
     4621 cgatatgctt cgtgatctgc tcaagaacgt tgatcttaaa gggttcgagc ctgatgtacg
     4681 tattttgctt accaaataca gcaatagcaa tggctctcag tccccgtgga tggaggagca
     4741 aattcgggat gcctggggaa gcatggttct aaaaaatgtt gtacgtgaaa cggatgaagt
     4801 tggtaaaggt cagatccgga tgagaactgt ttttgaacag gccattgatc aacgctcttc
     4861 aactggtgcc tggagaaatg ctctttctat ttgggaacct gtctgcaatg aaattttcga
     4921 tcgtctgatt aaaccacgct gggagattag ataatgaagc gtgcgcctgt tattccaaaa
     4981 catacgctca atactcaacc ggttgaagat acttcgttat cgacaccagc tgccccgatg
     5041 gtggattcgt taattgcgcg cgtaggagta atggctcgcg gtaatgccat tactttgcct
     5101 gtatgtggtc gggatgtgaa gtttactctt gaagtgctcc ggggtgatag tgttgagaag
     5161 acctctcggg tatggtcagg taatgaacgt gaccaggagc tgcttactga ggacgcactg
     5221 gatgatctca tcccttcttt tctactgact ggtcaacaga caccggcgtt cggtcgaaga
     5281 gtatctggtg tcatagaaat tgccgatggg agtcgccgtc gtaaagctgc tgcacttacc
     5341 gaaagtgatt atcgtgttct ggttggcgag ctggatgatg agcagatggc tgcattatcc
     5401 agattgggta acgattatcg cccacgtggt cagcgttatg caagccgatt gcagaatgaa
     5461 tttgctggaa atatttctgc gctggctgat gcggaaaata tttcacgtaa gattattacc
     5521 cgctgtatca acaccgccaa attgcctaaa tcagttgttg ctcttttttc tcaccccggt
     5581 gaactatctg cccggtcagg tgatgcactt caaaaagcct ttacagataa agaggaatta
     5641 cttaagcagc aggcatctaa ccttcatgag cagaaaaaag ctggggtgat atttgaagct
     5701 gaagaagtta tcactctttt aacttctgtg cttaaaacgt catctgcatc aagaactagt
     5761 ttaagctcac gacatcagtt tgctcctgga gcgacagtat tgtataaggg cgataaaatg
     5821 gtgcttaacc tggacaggtc tcgtgttcca actgagtgta tagagaaaat tgaggccatt
     5881 cttaaggaac ttgaaaagcc agcaccctga tgcgacctcg ttttagtcta cgtttatctg
     5941 tctttactta atgtcctttg ttacaggcca gaaagcataa ctggcctgaa tattctctct
     6001 gggcccactg ttccacttgt atcgtcggtc tgataatcag actgggacca cggtcccact
     6061 cgtatcgtcg gtctgattat tagtctggga ccacggtccc actcgtatcg tcggtctgat
     6121 tattagtctg ggaccacggt cccactcgta tcgtcggtct gattcagact gggaccacgg
     6181 tcccactcgt atcgtcggtc tgattattag tctgggacca cggtcccact cgtatcgtcg
     6241 gtctgattat tagtctggga ccacggtccc actcgtatcg tcggtctgat aatcagactg
     6301 ggaccacggt cccactcgta tcgtcggtct gattattagt ctgggaccat ggtcccactc
     6361 gtatcgtcgg tctgattatt agtctgggac cacggtccca ctcgtatcgt cggtctgatt
     6421 attagtctgg aaccacggtc ccactcgtat cgtcggtctg attattagtc tgggaccacg
     6481 gtcccactcg tatcgtcggt ctgattatta gtctgggacc acgatcccac tcgtgttgtc
     6541 ggtctgatta tcggtctggg accacggtcc cacttgtatt gtcgatcaga ctatcagcgt
     6601 gagactacga ttccatcaat gcctgtcaag ggcaagtatt gacatgtcgt cgtaacctgt
     6661 agaacggagt aacctcggtg tgcggttgta tgcctgctgt ggattgctgc tgtgtcctgc
     6721 ttatccacaa cattttgcgc acggttatgt ggacaaaata cctggttacc caggccgtgc
     6781 cggcacgtta accgggctgc atccgatgca agtgtgtcgc tgtcgacgag ctcgcgagct
     6841 cggacatgag gttgccccgt attcagtgtc gctgatttgt attgtctgaa gttgttttta
     6901 cgttaagttg atgcagatca attaatacga tacctgcgtc ataattgatt atttgacgtg
     6961 gtttgatggc ctccacgcac gttgtgatat gtagatgata atcattatca ctttacgggt
     7021 cctttccggt gatccgacag gttacggggc ggcgacctcg cgggttttcg ctatttatga
     7081 aaattttccg gtttaaggcg tttccgttct tcttcgtcat aacttaatgt ttttatttaa
     7141 aataccctct gaaaagaaag gaaacgacag gtgctgaaag cgagcttttt ggcctctgtc
     7201 gtttcctttc tctgtttttg tccgtggaat gaacaatgga agtccgagct catcgctaat
     7261 aacttcgtat agcatacatt atacgaagtt atattcgatg cggccgcaag gggttcgcgt
     7321 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
     7381 tgagagtgca ccatatgcgg tgtgaaatac cacacagatg cgtaaggaga aaataccgca
     7441 tcaggcgcca ttcgccattc agctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     7501 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     7561 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt aatacgactc
     7621 actatagggc gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg
     7681 caagcttgag tattctatag tctcacctaa atagcttggc gtaatcatgg tcatagctgt
     7741 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     7801 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     7861 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     7921 aaccccttgc ggccgcccgg gccgtcgacc aattctcatg tttgacagct tatcatcgaa
     7981 tttctgccat tcatccgctt attatcactt attcaggcgt agcaaccagg cgtttaaggg
     8041 caccaataac tgccttaaaa aaattacgcc ccgccctgcc actcatcgca gtactgttgt
     8101 aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc
     8161 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg
     8221 gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga
     8281 ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca
     8341 ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat
     8401 tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga
     8461 acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca
     8521 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt
     8581 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca
     8641 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta
     8701 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca
     8761 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc
     8821 cgatcaacgt ctcattttcg ccaaaagttg gcccagggct tcccggtatc aacagggaca
     8881 ccaggattta tttattctgc gaagtgatct tccgtcacag gtatttattc gcgataagct
     8941 catggagcgg cgtaaccgtc gcacaggaag gacagagaaa gcgcggatct gggaagtgac
     9001 ggacagaacg gtcaggacct ggattgggga ggcggttgcc gccgctgctg ctgacggtgt
     9061 gacgttctct gttccggtca caccacatac gttccgccat tcctatgcga tgcacatgct
     9121 gtatgccggt ataccgctga aagttctgca aagcctgatg ggacataagt ccatcagttc
     9181 aacggaagtc tacacgaagg tttttgcgct ggatgtggct gcccggcacc gggtgcagtt
     9241 tgtgatgccg gagtctgatg cggttgcgat gctgaaacaa ttatcctgag aataaatgcc
     9301 ttggccttta tatggaaatg tggaactgag tggatatgct gtttttgtct gttaaacaga
     9361 gaagctggct gttatccact gagaagcgaa cgaaacagtc gggaaaatct cccattatcg
     9421 tagagatccg cattattaat ctcaggagcc tgtgtagcgt ttataggaag tagtgttctg
     9481 tcatgatgcc tgcaagcggt aacgaaaacg atttgaatat gccttcagga acaatagaaa
     9541 tcttcgtgcg gtgttacgtt gaagtggagc ggattatgtc agcaatggac agaacaacct
     9601 aatgaacaca gaaccatgat gtggtctgtc cttttacagc cagtagtgct cgccgcagtc
     9661 gagcgacagg gcgaagccca tcgatactag cttgattggg atatctcgct catgtttgtc
     9721 gcgtgctatg tctttttagg tactttgaac ctacgttcgt acttgtataa tatgatcatc
     9781 gtattatcgt ttttcatccg tccagcgcaa aatgcattag cagctagtcc tagcgtgcgg
     9841 agctacctgg acaggtgcat gacggatgcg tgtccttcag tgactttcta attaacagta
     9901 acttctttac ttatgtttca gtttgtaaga agcgggattc gctcgtcgct tgacatctga
     9961 ttggactgcg tcggcacgtg aaaactacat tgtgaaatct gctaaaactc cgggtatctc
    10021 tgacacaaaa cgattcggct tcgcaatttc aacattacgg tcaaggctaa cgtatctttc
    10081 tcggtcaact tcagattatg ccgattaaat tgtcgtagct ttcaaggcgt tttgagtact
    10141 gcggcagttg ttgaacctgc aaggagaaga tctcgacaac agaataaagc gaaaaatggg
    10201 tctcatgcac taacactcag gcctccctca taatctctgt ttgagtttac caacaacaca
    10261 tatatacatt tcgacaaaat gaccactctt gacgacacgg cttaccggta ccgcaccagt
    10321 gtcccggggg acgccgaggc catcgaggca ctggatgggt ccttcaccac cgacaccgtc
    10381 ttccgcgtca ccgccaccgg ggacggcttc accctgcggg aggtgccggt ggacccgccc
    10441 ctgaccaagg tgttccccga cgacgaatcg gacgacgaat cggacgacgg ggaggacggc
    10501 gacccggact cccggacgtt cgtcgcgtac ggggacgacg gcgacctggc gggcttcgtg
    10561 gtcgtctcgt actccggctg gaaccgccgg ctgaccgtcg aggacatcga ggtcgccccg
    10621 gagcaccggg ggcacggggt cgggcgcgcg ttgatggggc tcgcgacgga gttcgcccgc
    10681 gagcggggcg ccgggcacct ctggctggag gtcaccaacg tcaacgcacc ggcgatccac
    10741 gcgtaccggc ggatggggtt caccctctgc ggcctggaca ccgccctgta cgacggcacc
    10801 gcctcggacg gcgagcaggc gctctacatg agcatgccct gcccctgacc gacgccgacc
    10861 aacaccgccg gtccgacgcg gcccgacggg tccgaggcct cggagatctg ggcccatgcg
    10921 gccgcaacaa ctacctcgac tttggctggg acactttcag tgaggacaag aagcttcaga
    10981 agcgtgctat cgaactcaac cagggacgtg cggcacaaat gggcatcctt gctctcatgg
    11041 tgcacgaaca gttggg
//
