LOCUS       sequence-474337-       12270 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A version of the episome pPtGE31_Delta-PtR with an added ShBle 
            cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12270)
  TITLE     Spheroplasted cells: a game changer for DNA delivery to diatoms
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pPtGE31_ShBle
FEATURES             Location/Qualifiers
     source          1..12270
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      575..794
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             885..1640
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    1643..1893
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     misc_feature    2055..2558
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        2559..2745
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             2746..3408
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     CDS             3598..4773
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             4773..5744
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    5817..6290
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    6550..6948
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     protein_bind    complement(6966..6999)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     7067..7086
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(7166..7441)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSYLGETIEYSSLHACRSTLEDPRVPSSNSPYSESYYNSL
                     AVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS"
     primer_bind     7279..7301
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7293..7310
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7294..7310
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7317..7336
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7317..7335
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    7338..7394
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(7437..7453)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7437..7453)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7450..7472)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7461..7477
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7485..7491,7492..7509,7510..7515))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7530..7551
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(7771..8430)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     8345..8364
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(8431..8533)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             9986..10555
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGFT
                     LREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNRR
                     LTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFTL
                     CGLDTALYDGTASDGEQALYMSMPCP"
     CDS             11507..11881
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      join(12155..12270,1..499)
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      join(12246..12270,1..507)
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 cggaaaactt ggccctcact gacagatgag gggcggacgt tgacacttga ggggccgact
       61 cacccggcgc ggcgttgaca gatgaggggc aggctcgatt tcggccggcg acgtggagct
      121 ggccagcctc gcaaatcggc gaaaacgcct gattttacgc gagtttccca cagatgatgt
      181 ggacaagcct ggggataagt gccctgcggt attgacactt gaggggcgcg actactgaca
      241 gatgaggggc gcgatccttg acacttgagg ggcagagtgc tgacagatga ggggcgcacc
      301 tattgacatt tgaggggctg tccacaggca gaaaatccag catttgcaag ggtttccgcc
      361 cgtttttcgg ccaccgctaa cctgtctttt aacctgcttt taaaccaata tttataaacc
      421 ttgtttttaa ccagggctgc gccctgtgcg cgtgaccgcg cacgccgaag gggggtgccc
      481 ccccttctcg aaccctcccg gtcgagtgag cgaggaagca ccagggaaca gcacttatat
      541 attctgctta cacacgatgc ctgaaaaaac ttcccttggg gttatccact tatccacggg
      601 gatattttta taattatttt ttttatagtt tttagatctt cttttttaga gcgccttgta
      661 ggcctttatc catgctggtt ctagagaagg tgttgtgaca aattgccctt tcagtgtgac
      721 aaatcaccct caaatgacag tcctgtctgt gacaaattgc ccttaaccct gtgacaaatt
      781 gccctcagaa gaagctgttt tttcacaaag ttatccctgc ttattgactc ttttttattt
      841 agtgtgacaa tctaaaaact tgtcacactt cacatggatc tgtcatggcg gaaacagcgg
      901 ttatcaatca caagaaacgt aaaaatagcc cgcgaatcgt ccagtcaaac gacctcactg
      961 aggcggcata tagtctctcc cgggatcaaa aacgtatgct gtatctgttc gttgaccaga
     1021 tcagaaaatc tgatggcacc ctacaggaac atgacggtat ctgcgagatc catgttgcta
     1081 aatatgctga aatattcgga ttgacctctg cggaagccag taaggatata cggcaggcat
     1141 tgaagagttt cgcggggaag gaagtggttt tttatcgccc tgaagaggat gccggcgatg
     1201 aaaaaggcta tgaatctttt ccttggttta tcaaacgtgc gcacagtcca tccagagggc
     1261 tttacagtgt acatatcaac ccatatctca ttcccttctt tatcgggtta cagaaccggt
     1321 ttacgcagtt tcggcttagt gaaacaaaag aaatcaccaa tccgtatgcc atgcgtttat
     1381 acgaatccct gtgtcagtat cgtaagccgg atggctcagg catcgtctct ctgaaaatcg
     1441 actggatcat agagcgttac cagctgcctc aaagttacca gcgtatgcct gacttccgcc
     1501 gccgcttcct gcaggtctgt gttaatgaga tcaacagcag aactccaatg cgcctctcat
     1561 acattgagaa aaagaaaggc cgccagacga ctcatatcgt attttccttc cgcgatatca
     1621 cttccatgac gacaggatag tctgagggtt atctgtcaca gatttgaggg tggttcgtca
     1681 catttgttct gacctactga gggtaatttg tcacagtttt gctgtttcct tcagcctgca
     1741 tggattttct catacttttt gaactgtaat ttttaaggaa gccaaatttg agggcagttt
     1801 gtcacagttg atttccttct ctttcccttc gtcatgtgac ctgatatcgg gggttagttc
     1861 gtcatcattg atgagggttg attatcacag tttattactc tgaattggct atccgcgtgt
     1921 gtacctctac ctggagtttt tcccacggtg gatatttctt cttgcgctga gcgtaagagc
     1981 tatctgacag aacagttctt ctttgcttcc tcgccagttc gctcgctatg ctcggttaca
     2041 cggctgcggc gagcatcacg tgctataaaa ataattataa tttaaatttt ttaatataaa
     2101 tatataaatt aaaaatagaa agtaaaaaaa gaaattaaag aaaaaatagt ttttgttttc
     2161 cgaagatgta aaagactcta gggggatcgc caacaaatac taccttttac cttgctcttc
     2221 ctgctctcag gtattaatgc cgaattgttt catcttgtct gtgtagaaga ccacacacga
     2281 aaatcctgtg attttacatt ttacttatcg ttaatcgaat gtatatctat ttaatctgct
     2341 tttcttgtct aataaatata tatgtaaagt acgctttttg ttgaaatttt ttaaaccttt
     2401 gtttattttt ttttcttcat tccgtaactc ttctaccttc tttatttact ttctaaaatc
     2461 caaatacaaa acataaaaat aaataaacac agagtaaatt cccaaattat tccatcatta
     2521 aaagatacga ggcgcgtgta agttacaggc aagcgatcct agtacactct atattttttt
     2581 atgcctcggt aatgattttc attttttttt tccacctagc ggatgactct ttttttttct
     2641 tagcgattgg cattatcaca taatgaatta tacattatat aaagtaatgt gatttcttcg
     2701 aagaatatac taaaaaatga gcaggcaaga taaacgaagg caaagatgac agagcagaaa
     2761 gccctagtaa agcgtattac aaatgaaacc aagattcaga ttgcgatctc tttaaagggt
     2821 ggtcccctag cgatagagca ctcgatcttc ccagaaaaag aggcagaagc agtagcagaa
     2881 caggccacac aatcgcaagt gattaacgtc cacacaggta tagggtttct ggaccatatg
     2941 atacatgctc tggccaagca ttccggctgg tcgctaatcg ttgagtgcat tggtgactta
     3001 cacatagacg accatcacac cactgaagac tgcgggattg ctctcggtca agcttttaaa
     3061 gaggccctag gggccgtgcg tggagtaaaa aggtttggat caggatttgc gcctttggat
     3121 gaggcacttt ccagagcggt ggtagatctt tcgaacaggc cgtacgcagt tgtcgaactt
     3181 ggtttgcaaa gggagaaagt aggagatctc tcttgcgaga tgatcccgca ttttcttgaa
     3241 agctttgcag aggctagcag aattaccctc cacgttgatt gtctgcgagg caagaatgat
     3301 catcaccgta gtgagagtgc gttcaaggct cttgcggttg ccataagaga agccacctcg
     3361 cccaatggta ccaacgatgt tccctccacc aaaggtgttc ttatgtagtt ttacacagga
     3421 gtctggactt gacgctagtg ataataagtg actgaggtat gtgctcttct tatctccttt
     3481 tgtagtgttg ctcttatttt aaacaacttt gcggtttttt gatgactttg cgattttgtt
     3541 gttgctttgc agtaaattgc aagatttaat aaaaaaacgc aaagcaatga ttaaaggatg
     3601 ttcagaatga aactcatgga aacacttaac cagtgcataa acgctggtca tgaaatgacg
     3661 aaggctatcg ccattgcaca gtttaatgat gacagcccgg aagcgaggaa aataacccgg
     3721 cgctggagaa taggtgaagc agcggattta gttggggttt cttctcaggc tatcagagat
     3781 gccgagaaag cagggcgact accgcacccg gatatggaaa ttcgaggacg ggttgagcaa
     3841 cgtgttggtt atacaattga acaaattaat catatgcgtg atgtgtttgg tacgcgattg
     3901 cgacgtgctg aagacgtatt tccaccggtg atcggggttg ctgcccataa aggtggcgtt
     3961 tacaaaacct cagtttctgt tcatcttgct caggatctgg ctctgaaggg gctacgtgtt
     4021 ttgctcgtgg aaggtaacga cccccaggga acagcctcaa tgtatcacgg atgggtacca
     4081 gatcttcata ttcatgcaga agacactctc ctgcctttct atcttgggga aaaggacgat
     4141 gtcacttatg caataaagcc cacttgctgg ccggggcttg acattattcc ttcctgtctg
     4201 gctctgcacc gtattgaaac tgagttaatg ggcaaatttg atgaaggtaa actgcccacc
     4261 gatccacacc tgatgctccg actggccatt gaaactgttg ctcatgacta tgatgtcata
     4321 gttattgaca gcgcgcctaa cctgggtatc ggcacgatta atgtcgtatg tgctgctgat
     4381 gtgctgattg ttcccacgcc tgctgagttg tttgactaca cctccgcact gcagtttttc
     4441 gatatgcttc gtgatctgct caagaacgtt gatcttaaag ggttcgagcc tgatgtacgt
     4501 attttgctta ccaaatacag caatagcaat ggctctcagt ccccgtggat ggaggagcaa
     4561 attcgggatg cctggggaag catggttcta aaaaatgttg tacgtgaaac ggatgaagtt
     4621 ggtaaaggtc agatccggat gagaactgtt tttgaacagg ccattgatca acgctcttca
     4681 actggtgcct ggagaaatgc tctttctatt tgggaacctg tctgcaatga aattttcgat
     4741 cgtctgatta aaccacgctg ggagattaga taatgaagcg tgcgcctgtt attccaaaac
     4801 atacgctcaa tactcaaccg gttgaagata cttcgttatc gacaccagct gccccgatgg
     4861 tggattcgtt aattgcgcgc gtaggagtaa tggctcgcgg taatgccatt actttgcctg
     4921 tatgtggtcg ggatgtgaag tttactcttg aagtgctccg gggtgatagt gttgagaaga
     4981 cctctcgggt atggtcaggt aatgaacgtg accaggagct gcttactgag gacgcactgg
     5041 atgatctcat cccttctttt ctactgactg gtcaacagac accggcgttc ggtcgaagag
     5101 tatctggtgt catagaaatt gccgatggga gtcgccgtcg taaagctgct gcacttaccg
     5161 aaagtgatta tcgtgttctg gttggcgagc tggatgatga gcagatggct gcattatcca
     5221 gattgggtaa cgattatcgc ccaacaagtg cttatgaacg tggtcagcgt tatgcaagcc
     5281 gattgcagaa tgaatttgct ggaaatattt ctgcgctggc tgatgcggaa aatatttcac
     5341 gtaagattat tacccgctgt atcaacaccg ccaaattgcc taaatcagtt gttgctcttt
     5401 tttctcaccc cggtgaacta tctgcccggt caggtgatgc acttcaaaaa gcctttacag
     5461 ataaagagga attacttaag cagcaggcat ctaaccttca tgagcagaaa aaagctgggg
     5521 tgatatttga agctgaagaa gttatcactc ttttaacttc tgtgcttaaa acgtcatctg
     5581 catcaagaac tagtttaagc tcacgacatc agtttgctcc tggagcgaca gtattgtata
     5641 agggcgataa aatggtgctt aacctggaca ggtctcgtgt tccaactgag tgtatagaga
     5701 aaattgaggc cattcttaag gaacttgaaa agccagcacc ctgatgcgac ctcgttttag
     5761 tctacgttta tctgtcttta cttaatgtcc tttgttacag gccagaaagc ataactggcc
     5821 tgaatattct ctctgggccc actgttccac ttgtatcgtc ggtctgataa tcagactggg
     5881 accacggtcc cactcgtatc gtcggtctga ttattagtct gggaccacgg tcccactcgt
     5941 atcgtcggtc tgattattag tctgggacca cggtcccact cgtatcgtcg gtctgataat
     6001 cagactggga ccacggtccc actcgtatcg tcggtctgat tattagtctg ggaccatggt
     6061 cccactcgta tcgtcggtct gattattagt ctgggaccac ggtcccactc gtatcgtcgg
     6121 tctgattatt agtctggaac cacggtccca ctcgtatcgt cggtctgatt attagtctgg
     6181 gaccacggtc ccactcgtat cgtcggtctg attattagtc tgggaccacg atcccactcg
     6241 tgttgtcggt ctgattatcg gtctgggacc acggtcccac ttgtattgtc gatcagacta
     6301 tcagcgtgag actacgattc catcaatgcc tgtcaagggc aagtattgac atgtcgtcgt
     6361 aacctgtaga acggagtaac ctcggtgtgc ggttgtatgc ctgctgtgga ttgctgctgt
     6421 gtcctgctta tccacaacat tttgcgcacg gttatgtgga caaaatacct ggttacccag
     6481 gccgtgccgg cacgttaacc gggctgcatc cgatgcaagt gtgtcgctgt cgacgagctc
     6541 gcgagctcgg acatgaggtt gccccgtatt cagtgtcgct gatttgtatt gtctgaagtt
     6601 gtttttacgt taagttgatg cagatcaatt aatacgatac ctgcgtcata attgattatt
     6661 tgacgtggtt tgatggcctc cacgcacgtt gtgatatgta gatgataatc attatcactt
     6721 tacgggtcct ttccggtgat ccgacaggtt acggggcggc gacctcgcgg gttttcgcta
     6781 tttatgaaaa ttttccggtt taaggcgttt ccgttcttct tcgtcataac ttaatgtttt
     6841 tatttaaaat accctctgaa aagaaaggaa acgacaggtg ctgaaagcga gctttttggc
     6901 ctctgtcgtt tcctttctct gtttttgtcc gtggaatgaa caatggaagt ccgagctcat
     6961 cgctaataac ttcgtatagc atacattata cgaagttata ttcgatgcgg ccgcaagggg
     7021 ttcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     7081 attgtactga gagtgcacca tatgcggtgt gaaataccac acagatgcgt aaggagaaaa
     7141 taccgcatca ggcgccattc gccattcagc tgcgcaactg ttgggaaggg cgatcggtgc
     7201 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     7261 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat
     7321 acgactcact atagggcgaa ttcgagctcg gtacccgggg atcctctaga gtcgacctgc
     7381 aggcatgcaa gcttgagtat tctatagtct cacctaaata gcttggcgta atcatggtca
     7441 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     7501 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     7561 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     7621 caacgcgaac cccttgcggc cgcccgggcc gtcgaccaat tctcatgttt gacagcttat
     7681 catcgaattt ctgccattca tccgcttatt atcacttatt caggcgtagc aaccaggcgt
     7741 ttaagggcac caataactgc cttaaaaaaa ttacgccccg ccctgccact catcgcagta
     7801 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac
     7861 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
     7921 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
     7981 ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag
     8041 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
     8101 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
     8161 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg
     8221 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
     8281 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
     8341 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
     8401 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
     8461 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct
     8521 tacgtgccga tcaacgtctc attttcgcca aaagttggcc cagggcttcc cggtatcaac
     8581 agggacacca ggatttattt attctgcgaa gtgatcttcc gtcacaggta tttattcgcg
     8641 ataagctcat ggagcggcgt aaccgtcgca caggaaggac agagaaagcg cggatctggg
     8701 aagtgacgga cagaacggtc aggacctgga ttggggaggc ggttgccgcc gctgctgctg
     8761 acggtgtgac gttctctgtt ccggtcacac cacatacgtt ccgccattcc tatgcgatgc
     8821 acatgctgta tgccggtata ccgctgaaag ttctgcaaag cctgatggga cataagtcca
     8881 tcagttcaac ggaagtctac acgaaggttt ttgcgctgga tgtggctgcc cggcaccggg
     8941 tgcagtttgt gatgccggag tctgatgcgg ttgcgatgct gaaacaatta tcctgagaat
     9001 aaatgccttg gcctttatat ggaaatgtgg aactgagtgg atatgctgtt tttgtctgtt
     9061 aaacagagaa gctggctgtt atccactgag aagcgaacga aacagtcggg aaaatctccc
     9121 attatcgtag agatccgcat tattaatctc aggagcctgt gtagcgttta taggaagtag
     9181 tgttctgtca tgatgcctgc aagcggtaac gaaaacgatt tgaatatgcc ttcaggaaca
     9241 atagaaatct tcgtgcggtg ttacgttgaa gtggagcgga ttatgtcagc aatggacaga
     9301 acaacctaat gaacacagaa ccatgatgtg gtctgtcctt ttacagccag tagtgctcgc
     9361 cgcagtcgag cgacagggcg aagcccatcg atactagctt gattgggata tctcgctcat
     9421 gtttgtcgcg tgctatgtct ttttaggtac tttgaaccta cgttcgtact tgtataatat
     9481 gatcatcgta ttatcgtttt tcatccgtcc agcgcaaaat gcattagcag ctagtcctag
     9541 cgtgcggagc tacctggaca ggtgcatgac ggatgcgtgt ccttcagtga ctttctaatt
     9601 aacagtaact tctttactta tgtttcagtt tgtaagaagc gggattcgct cgtcgcttga
     9661 catctgattg gactgcgtcg gcacgtgaaa actacattgt gaaatctgct aaaactccgg
     9721 gtatctctga cacaaaacga ttcggcttcg caatttcaac attacggtca aggctaacgt
     9781 atctttctcg gtcaacttca gattatgccg attaaattgt cgtagctttc aaggcgtttt
     9841 gagtactgcg gcagttgttg aacctgcaag gagaagatct cgacaacaga ataaagcgaa
     9901 aaatgggtct catgcactaa cactcaggcc tccctcataa tctctgtttg agtttaccaa
     9961 caacacatat atacatttcg acaaaatgac cactcttgac gacacggctt accggtaccg
    10021 caccagtgtc ccgggggacg ccgaggccat cgaggcactg gatgggtcct tcaccaccga
    10081 caccgtcttc cgcgtcaccg ccaccgggga cggcttcacc ctgcgggagg tgccggtgga
    10141 cccgcccctg accaaggtgt tccccgacga cgaatcggac gacgaatcgg acgacgggga
    10201 ggacggcgac ccggactccc ggacgttcgt cgcgtacggg gacgacggcg acctggcggg
    10261 cttcgtggtc gtctcgtact ccggctggaa ccgccggctg accgtcgagg acatcgaggt
    10321 cgccccggag caccgggggc acggggtcgg gcgcgcgttg atggggctcg cgacggagtt
    10381 cgcccgcgag cggggcgccg ggcacctctg gctggaggtc accaacgtca acgcaccggc
    10441 gatccacgcg taccggcgga tggggttcac cctctgcggc ctggacaccg ccctgtacga
    10501 cggcaccgcc tcggacggcg agcaggcgct ctacatgagc atgccctgcc cctgaccgac
    10561 gccgaccaac accgccggtc cgacgcggcc cgacgggtcc gaggcctcgg agatctgggc
    10621 ccatgcggcc gcaacaacta cctcgacttt ggctgggaca ctttcagtga ggacaagaag
    10681 cttcagaagc gtgctatcga actcaaccag ggacgtgcgg cacaaatggg catccttgct
    10741 ctcatggtgc acgaacagtt gggagtctct atccttcctt aaaaatttaa ttttcattag
    10801 ttgcagtcac tccgctttgg tttcaggatt agtgcaattc gagttgaatc actgggaaaa
    10861 acattgtctt cttttttata ttatcatttg cattagtgct gcagtcgtag atacttgttg
    10921 gttgaaagac atcagctggg agggactgga ctagcgtttg gtaaggagac atacctgtta
    10981 acgttggttg caaaattcca tttcgcgatt tatgttatct gtaaatcctg atttgtctgg
    11041 aattcttgat acttccgttt ttttagaggc caatgattag catcggcgat tctcaaaata
    11101 gcattttcga catgcggtgc tgatttcata aacatagaca acgcttttac atgtaaaagt
    11161 aacttgcgga cttggaacag tgctctgttt ttggtgtgaa cgtaactcag caatatttct
    11221 gtgctagcaa ggttttttat gatcgaccga agatctcaaa actccgggtc tttcaactgt
    11281 ctgactagac catgttcgta acgtcggaca gcagctttcg ttgtactggt agaatttcta
    11341 cgtgcgaagc acgtgtaggc aggttgaacg acgatccctg ccgatggatg gattggcacg
    11401 cggcggaacg ctttcgtgat ctacaccacc tggatcttca catatcttcg aaatcgaaaa
    11461 attaaccaag tcgacggtat cgataatatt ctagctgagg gtacccatgg ccaagttgac
    11521 cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga
    11581 ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga
    11641 cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg
    11701 ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa
    11761 cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga
    11821 gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg
    11881 accgacgccg accaacaccg ccggtccgac gcggcccgac gggtccgagg cctcggagat
    11941 ctgggcccat gcggccgcaa caactacctc gactttggct gggacacttt cagtgaggac
    12001 aagaagcttc agaagcgtgc tatcgaactc aaccagggac gtgcggcaca aatgggcatc
    12061 cttgctctca tggtgcacga acagttggga gtctctatcc ttccttaaaa atttaatttt
    12121 cattagttgc agtcactccg ctttggtttt cgagctggtt gccctcgccg ctgggctggc
    12181 ggccgtctat ggccctgcaa acgcgccaga aacgccgtcg aagccgtgtg cgagacaccg
    12241 cggccggccg ccggcgttgt ggatacctcg
//
