LOCUS       sequence-47434-e        9091 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9091)
  AUTHORS   Euskirchen P, Skaftnesmo KO, Huszthy PC, Brekka N, Bjerkvig R, 
            Jacobs AH, Miletic H
  TITLE     NUMB does not impair growth and differentiation status of 
            experimental gliomas.
  JOURNAL   Exp Cell Res. 2011 Dec 10;317(20):2864-73. Epub 2011 Sep 12.
  PUBMED    21939656
REFERENCE   2  (bases 1 to 9091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp Cell 
            Res."; date: "2011-12-10"; volume: "317(20)"; pages: "2864-73. Epub 
            2011 Sep 12"
COMMENT     Sequence Label: NUMB4-eGFP-M107K
FEATURES             Location/Qualifiers
     source          1..9091
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1803..1812
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1809..1811,1812..1814,1815..2528)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1809..1811,1812..1814,1815..2525)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1854..1875)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2115..2134)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2462..2483
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2544..3132
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2597..2617)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3015..3026)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3218..3451
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3523..3644
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3560..3579)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3614..3633
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3663..3798
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3830..3849)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3831..3849)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3859..3876)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3859..3875)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3868..3890)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4020..4475
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4107..4126)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4317..4338
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4504..4608
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4609..4677,4678..5469)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4827..4846)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5640..6228
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6129..6148
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6382..6399
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6516..6537
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6552..6557,6558..6575,6576..6582)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6590..6606
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6595..6617
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6614..6630
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6614..6630
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6649..6669
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6651..6669
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             6925..7105
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7149..7274
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7767..8000
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8184..8228
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        8507..8810
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8811..9014
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8965..8985
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9011..9035
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gatctgccac catgaacaaa ttacggcaaa gttttaggag aaagaaggat gtttatgttc
       61 cagaggccag tcgtccacat cagtggcaga cagatgaaga aggcgttcgc accggaaaat
      121 gtagcttccc ggttaagtac cttggccatg tagaagttga tgaatcaaga ggaatgcaca
      181 tctgtgaaga tgctgtaaaa agattgaaag ctactggaaa gaaagcagtt aaagcagttc
      241 tgtgggtctc agcagatgga ctcagagttg tggatgaaaa aactaaggac ctcatagttg
      301 accagacgat agagaaagtt tctttctgtg ccccagacag gaactttgat agagcctttt
      361 cttacatatg ccgtgatggc accactcgtc gctggatctg tcactgcttc atggctgtca
      421 aggacacagg tgaaaggttg agccatgcag taggctgtgc ttttgcagcc tgtttagagc
      481 gcaagcagaa gcgggagaag gaatgtggag tgactgctac ttttgatgct agtcggacca
      541 cttttacaag agaaggatca ttccgtgtca caacagccac tgaacaagca gaaagagagg
      601 agatcatgaa acaaatgcaa gatgccaaga aagctgaaac agataagata gtcgttggtt
      661 catcagttgc ccctggcaac actgccccat ccccatcctc tcccacctct cctacttctg
      721 atgccacgac ctctctggag atgaacaatc ctcatgccat cccacgccgg catgctccaa
      781 ttgaacagct tgctcgccaa ggctctttcc gaggttttcc tgctcttagc cagaagatgt
      841 caccctttaa acgccaacta tccctacgca tcaatgagtt gccttccact atgcagagga
      901 agactgattt ccccattaaa aatgcagtgc cagaagtaga aggggaggca gagagcatca
      961 gctccctgtg ctcacagatc accaatgcct tcagcacacc tgaggacccc ttctcatctg
     1021 ctccgatgac caaaccagtg acagtggtgg caccacaatc tcctaccttc caagggaccg
     1081 agtggggtca atcttctggt gctgcctctc caggtctctt ccaggccggt catagacgta
     1141 ctccctctga ggccgaccga tggttagaag aggtgtctaa gagcgtccgg gctcagcagc
     1201 cccaggcctc agctgctcct ctgcagccag ttctccagcc tcctccaccc actgccatct
     1261 cccagccagc atcacctttc caagggaatg cattcctcac ctctcagcct gtgccagtgg
     1321 gtgtggtccc agccctgcaa ccagcctttg tccctgccca gtcctatcct gtggccaatg
     1381 gaatgcccta tccagcccct aatgtgcctg tggtgggcat cactccctcc cagatggtgg
     1441 ccaacgtatt tggcactgca ggccaccctc aggctgccca tccccatcag tcacccagcc
     1501 tggtcaggca gcagacattc cctcactacg aggcaagcag tgctaccacc agtcccttct
     1561 ttaagcctcc tgctcagcac ctcaacggtt ctgcagcttt caatggtgta gatgatggca
     1621 ggttggcctc agcagacagg catacagagg ttcctacagg cacctgccca gtggatcctt
     1681 ttgaagccca gtgggctgca ttagaaaata agtccaagca gcgtactaat ccctccccta
     1741 ccaacccttt ctccagtgac ttacagaaga cgtttgaaat tgaacttgca gatccaccgg
     1801 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     1861 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     1921 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     1981 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     2041 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     2101 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     2161 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     2221 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     2281 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     2341 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     2401 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     2461 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     2521 acaagtaaag cggccgcgtc gacaatcaac ctctggatta caaaatttgt gaaagattga
     2581 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     2641 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     2701 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     2761 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     2821 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     2881 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
     2941 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     3001 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     3061 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     3121 ccgcctcccc gcctgaatac gagctcggta cctttaagac caatgactta caaggcagct
     3181 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     3241 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     3301 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     3361 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     3421 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     3481 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     3541 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     3601 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     3661 ctatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     3721 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     3781 gaggcttttt tggaggccta ggcttttgcg tcgagacgta cccaattcgc cctatagtga
     3841 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     3901 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     3961 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcga
     4021 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     4081 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     4141 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     4201 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     4261 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     4321 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     4381 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     4441 cgcgaatttt aacaaaatat taacgtttac aatttcccag gtggcacttt tcggggaaat
     4501 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     4561 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     4621 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     4681 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     4741 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     4801 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     4861 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     4921 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     4981 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     5041 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     5101 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     5161 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     5221 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     5281 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     5341 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     5401 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     5461 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     5521 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     5581 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     5641 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     5701 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     5761 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     5821 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     5881 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     5941 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     6001 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     6061 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     6121 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     6181 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     6241 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     6301 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     6361 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     6421 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     6481 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     6541 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     6601 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
     6661 cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt
     6721 agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc
     6781 gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga
     6841 cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta
     6901 agtgcctagc tcgatacaat aaacgggtct ctctggttag accagatctg agcctgggag
     6961 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     7021 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     7081 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacctgaaa gcgaaaggga
     7141 aaccagagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     7201 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     7261 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     7321 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     7381 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     7441 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     7501 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     7561 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
     7621 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     7681 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     7741 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     7801 ggaagcacta tgggcgcagc ctcaatgacg ctgacggtac aggccagaca attattgtct
     7861 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     7921 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     7981 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     8041 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagattgg aatcacacga
     8101 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     8161 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     8221 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     8281 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     8341 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     8401 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     8461 gattagtgaa cggatctcga cggtatcgat aagcttggga gttccgcgtt acataactta
     8521 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     8581 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     8641 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     8701 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     8761 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     8821 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     8881 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     8941 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     9001 atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt
     9061 ttgacctcca tagaagacac cgactctaga g
//
