LOCUS       sequence-474357-       10187 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid pQdCas12a.empty expresses the dCas12a endonuclease..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10187)
  AUTHORS   Golla SA, Abo-Hashesh M, Gupta D, Liu Y, Mahadevan R
  TITLE     Model-Based Optimization of a qCRISPRi Circuit for Dynamic Control 
            of Metabolic Pathways.
  JOURNAL   ACS Synth Biol. 2025 Oct 17;14(10):3890-3898. doi: 
            10.1021/acssynbio.5c00095. Epub 2025 Sep 29.
  PUBMED    41021780
REFERENCE   2  (bases 1 to 10187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5c00095"; journalName: "ACS Synth Biol"; date: 
            "2025-10-17- 17"; volume: "14"; issue: "10"; pages: "3890-3898"
COMMENT     Sequence Label: pQdCas12a.empty
FEATURES             Location/Qualifiers
     source          1..10187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             402..1196
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(456..475)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1066..1085
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     RBS             2934..2945
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        6861..6895
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     CDS             complement(6963..7259)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSG"
     primer_bind     7109..7131
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7123..7140
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7124..7140
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7150..7169
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7150..7168
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7216..7236)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7216..7234)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7255..7271)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7255..7271)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7268..7290)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7279..7295
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7303..7309,7310..7327,7328..7333))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7348..7369
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(7675..8337)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGE*QLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      8338..9109
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 atgcaggcca aggccgccgc gatcatcaag gccgtgggcg aaaagctgct gacggaacag
       61 cgggaagtcc agcgccagaa acaggcccag cgccagcagg aacgcgggcg cgcacatttc
      121 cccgaaaagt gccacctggg atgaatgtca gctactgggc tatctggaca agggaaaacg
      181 caagcgcaaa gagaaagcag gtagcttgca gtgggcttac atggcgatag ctagactggg
      241 cggttttatg gacagcaagc gaaccggaat tgccagctgg ggcgccctct ggtaaggttg
      301 ggaagccctg caaagtaaac tggatggctt tcttgccgcc aaggatctga tggcgcaggg
      361 gatcaagatt tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
      421 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
      481 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
      541 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact acaggacgag gcagcgcggc
      601 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
      661 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
      721 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
      781 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
      841 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
      901 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
      961 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     1021 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     1081 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     1141 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     1201 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     1261 ttccaccgcc gccttctatg aaaggttgga ctagagaatt catcctgaac ttatctagat
     1321 taatttaaga ctgctttttt aaactgttca ttaataggca tagacaatac aaccgattta
     1381 gtatcaccta atacatgaat ttctttgtct ccaatacgat gacaaggaac tttaatacgc
     1441 tttaaaaatc gctctattgc tgttgatgtt actgttacat attctgtaat accttgacta
     1501 acagcgtgtt tatatatagc ttcaaatagt ttcattgtaa tttcactagc agagttattt
     1561 atctttgagc tatttttacc tacagcaaaa cgacttaatt cgactatatt aggatctttg
     1621 ggagcactct gttgaccaag caattcagga aaaacacttt tcagcatata atcacctgtt
     1681 gtaggtaata aacgccagca tccacttaca ttttcagtat catcacaagc ataaatatat
     1741 tctgcatttg agttatcata ctcatctgat tcaaggttat tttctacaac taagtcccac
     1801 tcaagtcttt gcttaaacac ttgataacga agacttagaa tacctttata ctcctccgat
     1861 ggaattgcca aaaaatccga tttttttatc attatagtca taccaacctc ccttgcgttt
     1921 attcgactat aacaaaccat tttcttgcgt aaacctgtac gatcctacag gtgcttatgt
     1981 taagtaattg tattcccagc gatacaatag tgtgacaaaa atccaattta ttagaatcaa
     2041 atgtcaatcc attaccgttt taatgatata taacacgcaa aacttgcgac aaacaatagg
     2101 taaggataaa gagatgggta tgaaaaacat aaatgccgac gacacataca gaataattaa
     2161 taaaattaaa gcttgtagaa gcaataatga tattaatcaa tgcttatctg atatgactaa
     2221 aatggtacat tgtgaatatt atttactcgc gatcatttat cctcattcta tggttaaatc
     2281 tgatatttca attctagata attaccctaa aaaatggagg caatattatg atgacgctaa
     2341 tttaataaaa tatgatccta tagtagatta ttctaactcc aatcattcac caattaattg
     2401 gaatatattt gaaaacaatg ctgtaaataa aaaatctcca aatgtaatta aagaagcgaa
     2461 aacatcaggt cttatcactg ggtttagttt ccctattcat acggctaaca atggcttcgg
     2521 aatgcttagt tttgcacatt cagaaaaaga caactatata gatagtttat ttttacatgc
     2581 gtgtatgaac ataccattaa ttgttccttc tctagttgat aattatcgaa aaataaatat
     2641 agcaaataat aaatcaaaca acgatttaac caaaagagaa aaagaatgtt tagcgtgggc
     2701 atgcgaagga aaaagctctt gggatatttc aaaaatatta ggctgcagtg agcgtactgt
     2761 cactttccat ttaaccaatg cgcaaatgaa actcaataca acaaaccgct gccaaagtat
     2821 ttctaaagca attttaacag gagcaattga ttgcccatac tttaaaaatt aaagatctac
     2881 ctgtaggatc gtacaggttt acgcaagaaa atggtttgtt atagtcgaat aaaaaagagg
     2941 agaaaggatc tatgtcaatt tatcaagaat ttgttaataa atatagttta agtaaaactc
     3001 taagatttga gttaatccca cagggtaaaa cacttgaaaa cataaaagca agaggtttga
     3061 ttttagatga tgagaaaaga gctaaagact acaaaaaggc taaacaaata attgataaat
     3121 atcatcagtt ttttatagag gagatattaa gttcggtttg tattagcgaa gatttattac
     3181 aaaactattc tgatgtttat tttaaactta aaaagagtga tgatgataat ctacaaaaag
     3241 attttaaaag tgcaaaagat acgataaaga aacaaatatc tgaatatata aaggactcag
     3301 agaaatttaa gaatttgttt aatcaaaacc ttatcgatgc taaaaaaggg caagagtcag
     3361 atttaattct atggctaaag caatctaagg ataatggtat agaactattt aaagccaata
     3421 gtgatatcac agatatagat gaggcgttag aaataatcaa atcttttaaa ggttggacaa
     3481 cttattttaa gggttttcat gaaaatagaa aaaatgttta tagtagcaat gatattccta
     3541 catctattat ttataggata gtagatgata atttgcctaa atttctagaa aataaagcta
     3601 agtatgagag tttaaaagac aaagctccag aagctataaa ctatgaacaa attaaaaaag
     3661 atttggcaga agagctaacc tttgatattg actacaaaac atctgaagtt aatcaaagag
     3721 ttttttcact tgatgaagtt tttgagatag caaactttaa taattatcta aatcaaagtg
     3781 gtattactaa atttaatact attattggtg gtaaatttgt aaatggtgaa aatacaaaga
     3841 gaaaaggtat aaatgaatat ataaatctat actcacagca aataaatgat aaaacactca
     3901 aaaaatataa aatgagtgtt ttatttaagc aaattttaag tgatacagaa tctaaatctt
     3961 ttgtaattga taagttagaa gatgatagtg atgtagttac aacgatgcaa agtttttatg
     4021 agcaaatagc agcttttaaa acagtagaag aaaaatctat taaagaaaca ctatctttat
     4081 tatttgatga tttaaaagct caaaaacttg atttgagtaa aatttatttt aaaaatgata
     4141 aatctcttac tgatctatca caacaagttt ttgatgatta tagtgttatt ggtacagcgg
     4201 tactagaata tataactcaa caaatagcac ctaaaaatct tgataaccct agtaagaaag
     4261 agcaagaatt aatagccaaa aaaactgaaa aagcaaaata cttatctcta gaaactataa
     4321 agcttgcctt agaagaattt aataagcata gagatataga taaacagtgt aggtttgaag
     4381 aaatacttgc aaactttgcg gctattccga tgatatttga tgaaatagct caaaacaaag
     4441 acaatttggc acagatatct atcaaatatc aaaatcaagg taaaaaagac ctacttcaag
     4501 ctagtgcgga agatgatgtt aaagctatca aggatctttt agatcaaact aataatctct
     4561 tacataaact aaaaatattt catattagtc agtcagaaga taaggcaaat attttagaca
     4621 aggatgagca tttttatcta gtatttgagg agtgctactt tgagctagcg aatatagtgc
     4681 ctctttataa caaaattaga aactatataa ctcaaaagcc atatagtgat gagaaattta
     4741 agctcaattt tgagaactcg actttggcta atggttggga taaaaataaa gagcctgaca
     4801 atacggcaat tttatttatc aaagatgata aatattatct gggtgtgatg aataagaaaa
     4861 ataacaaaat atttgatgat aaagctatca aagaaaataa aggcgagggt tataaaaaaa
     4921 ttgtttataa acttttacct ggcgcaaata aaatgttacc taaggttttc ttttctgcta
     4981 aatctataaa attttataat cctagtgaag atatacttag aataagaaat cattccacac
     5041 atacaaaaaa tggtagtcct caaaaaggat atgaaaaatt tgagtttaat attgaagatt
     5101 gccgaaaatt tatagatttt tataaacagt ctataagtaa gcatccggag tggaaagatt
     5161 ttggatttag attttctgat actcaaagat ataattctat agatgaattt tatagagaag
     5221 ttgaaaatca aggctacaaa ctaacttttg aaaatatatc agagagctat attgatagcg
     5281 tagttaatca gggtaaattg tacctattcc aaatctataa taaagatttt tcagcttata
     5341 gcaaagggcg accaaatcta catactttat attggaaagc gctgtttgat gagagaaatc
     5401 ttcaagatgt ggtttataag ctaaatggtg aggcagagct tttttatcgt aaacaatcaa
     5461 tacctaaaaa aatcactcac ccagctaaag aggcaatagc taataaaaac aaagataatc
     5521 ctaaaaaaga gagtgttttt gaatatgatt taatcaaaga taaacgcttt actgaagata
     5581 agtttttctt tcactgtcct attacaatca attttaaatc tagtggagct aataagttta
     5641 atgatgaaat caatttattg ctaaaagaaa aagcaaatga tgttcatata ttaagtatag
     5701 ctagaggtga aagacattta gcttactata ctttggtaga tggtaaaggc aatatcatca
     5761 aacaagatac tttcaacatc attggtaatg atagaatgaa aacaaactac catgataagc
     5821 ttgctgcaat agagaaagat agggattcag ctaggaaaga ctggaaaaag ataaataaca
     5881 tcaaagagat gaaagagggc tatctatctc aggtagttca tgaaatagct aagctagtta
     5941 tagagtataa tgctattgtg gtttttgagg atttaaattt tggatttaaa agagggcgtt
     6001 tcaaggtaga gaagcaggtc tatcaaaagt tagaaaaaat gctaattgag aaactaaact
     6061 atctagtttt caaagataat gagtttgata aaactggggg agtgcttaga gcttatcagc
     6121 taacagcacc ttttgagact tttaaaaaga tgggtaaaca aacaggtatt atctactatg
     6181 taccagctgg ttttacttca aaaatttgtc ctgtaactgg ttttgtaaat cagttatatc
     6241 ctaagtatga aagtgtcagc aaatctcaag agttctttag taagtttgac aagatttgtt
     6301 ataaccttga taagggctat tttgagttta gttttgatta taaaaacttt ggtgacaagg
     6361 ctgccaaagg caagtggact atagctagct ttgggagtag attgattaac tttagaaatt
     6421 cagataaaaa tcataattgg gatactcgag aagtttatcc aactaaagag ttggagaaat
     6481 tgctaaaaga ttattctatc gaatatgggc atggcgaatg tatcaaagca gctatttgcg
     6541 gtgagagcga caaaaagttt tttgctaagc taactagtgt cctaaatact atcttacaaa
     6601 tgcgtaactc aaaaacaggt actgagttag attatctaat ttcaccagta gcagatgtaa
     6661 atggcaattt ctttgattcg cgacaggcgc caaaaaatat gcctcaagat gctgatgcca
     6721 atggtgctta tcatattggg ctaaaaggtc tgatgctact aggtaggatc aaaaataatc
     6781 aagagggcaa aaaactcaat ttggttatca aaaatgaaga gtattttgag ttcgtgcaga
     6841 ataggaataa ctaactcgag ttgacagcta gctcagtcct aggtataatg ctagcgctga
     6901 tttaggcaaa aacgggtcta agaactttaa ataatttcta ctgttgtaga tgagagcagg
     6961 tgtcagccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg
     7021 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
     7081 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
     7141 gagcgcgcgt aatacgactc actatagggc gaattgggta ccgggccccc cctcgaggtc
     7201 ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata
     7261 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     7321 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     7381 ctcactgccc gctttccacc cacctgcaaa agtctaagaa ctttaaataa tttctactgt
     7441 tgtagatgct actattcctg tgccttcaga taattcagtc taagaacttt aaataatttc
     7501 tactgttgta gatgtctaga gccttttgta ttagtagccg gtctaagaac tttaaataat
     7561 ttctactgtt gtagattagc gatttatgaa ggtcattttt tttgttaact tcaggacctg
     7621 cagtctagta tagtctggaa cagcgcactt acgggttgct gcgcaaccca agtgctaccg
     7681 gcgcggcagc gtgacccgtg tcggcggctc caacggctcg ccatcgtcca gaaaacacgg
     7741 ctcatcgggc atcggcaggc gctgctgccc gcgccgttcc cattcctccg tttcggtcaa
     7801 ggctggcagg tctggttcca tgcccggaat gccgggctgg ctgggcggct cctcgccggg
     7861 gccggtcggt agttgctact cgcccggata cagggtcggg atgcggcgca ggtcgccatg
     7921 ccccaacagc gattcgtcct ggtcgtcgtg atcaaccacc acggcggcac tgaacaccga
     7981 caggcgcaac tggtcgcggg gctggcccca cgccacgcgg tcattgacca cgtaggccga
     8041 cacggtgccg gggccgttga gcttcacgac ggagatccag cgctcggcca ccaagtcctt
     8101 gactgcgtat tggaccgtcc gcaaagaacg tccgatgagc ttggaaagtg tcttctggct
     8161 gaccaccacg gcgttctggt ggcccatctg cgccacgagg tgatgcagca gcattgccgc
     8221 cgtgggtttc ctcgcaataa gcccggccca cgcctcatgc gctttgcgtt ccgtttgcac
     8281 ccagtgaccg ggcttgttct tggcttgaat gccgatttct ctggactgcg tggccatgct
     8341 tatctccatg cggtaggggt gccgcacggt tgcggcacca tgcgcaatca gctgcaactt
     8401 tttggcagcg cgacaacaat tatgcgttgc gtaaaagtgg cagtcaatta cagattttct
     8461 ttaacctacg caatgagcta ttgcgggggg tgccgcaatg agctgttgcg tacccccctt
     8521 ttttaagttg ttgattttta agtctttcgc atttcgccct atatctagtt ctttggtgcc
     8581 caaagaaggg cacccctgcg gggttccccc acgccttcgg cgcggctccc cctccggcaa
     8641 aaagtggccc ctccggggct tgttgatcga ctgcgcggcc ttcggccttg cccaaggtgg
     8701 cgctgccccc ttggaacccc cgcactcgcc gccgtgaggc tcggggggca ggcgggcggg
     8761 cttcgccctt cgactgcccc cactcgcata ggcttgggtc gttccaggcg cgtcaaggcc
     8821 aagccgctgc gcggtcgctg cgcgagcctt gacccgcctt ccacttggtg tccaaccggc
     8881 aagcgaagcg cgcaggccgc aggccggagg cttttcccca gagaaaatta aaaaaattga
     8941 tggggcaagg ccgcaggccg cgcagttgga gccggtgggt atgtggtcga aggctgggta
     9001 gccggtgggc aatccctgtg gtcaagctcg tgggctggcg cagcctgtcc atcagcttgt
     9061 ccagcagggt tgtccacggg ccgagcgaag cgagccagcc ggtggccgct cgcggccatc
     9121 gtccacatat ccacgggctg gcaagggagc gcagcgaccg cgcagggcga agcccggaga
     9181 gcaagcccgt agggcgccgc agccgccgta ggcggtcacg actttgcgaa gcaaagtcta
     9241 gtgagtatac tcaagcattg agtggcccgc cggaggcacc gccttgcgct gcccccgtcg
     9301 agccggttgg acaccaaaag ggaggggcag gcatggcggc atacgcgatc atgcgatgca
     9361 agaagctggc gaaaatgggc aacgtggcgg ccagtctcaa gcacgcctac cgcgagcgcg
     9421 agacgcccaa cgctgacgcc agcaggacgc cagagaacga gcactgggcg gccagcagca
     9481 ccgatgaagc gatgggccga ctgcgcgagt tgctgccaga gaagcggcgc aaggacgctg
     9541 tgttggcggt cgagtacgtc atgacggcca gcccggaatg gtggaagtcg gccagccaag
     9601 aacagcaggc ggcgttcttc gagaaggcgc acaagtggct ggcggacaag tacggggcgg
     9661 atcgcatcgt gacggccagc atccaccgtg acgaaaccag cccgcacatg accgcgttcg
     9721 tggtgccgct gacgcaggac ggcaggctgt cggccaagga gttcatcggc aacaaagcgc
     9781 agatgacccg cgaccagacc acgtttgcgg ctgctgtggc cgatctaggg ctgcaacggg
     9841 gcatcgaggg cagcaaggca cgtcacacgc gcattcaggc gttctacgag gccctggagc
     9901 ggccaccagt gggccacgtc accatcagcc cgcaagcggt cgagccacgc gcctatgcac
     9961 cgcagggatt ggccgaaaag ctgggaatct caaagcgcgt tgagacgccg gaagccgtgg
    10021 ccgaccggct gacaaaagcg gttcggcagg ggtatgagcc tgccctacag gccgccgcag
    10081 gagcgcgtga gatgcgcaag aaggccgatc aagcccaaga gacggcccga gaccttcggg
    10141 agcgcctgaa gcccgttctg gacgccctgg ggccgttgaa tcgggat
//
