LOCUS       sequence-474366-       10916 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For overexpression of EGFP-BRD4-NUT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10916)
  AUTHORS   Zhang Y, Stoppelkamp I, Fernandez-Pernas P, Allram M, Charman M, 
            Magalhaes AP, Piedavent-Salomon M, Sommer G, Sung YC, Meyer K, Grams
            N, Halko E, Dongre S, Meierhofer D, Malszycki M, Ilik IA, Aktas T, 
            Kraushar ML, Vastenhouw N, Weitzman MD, Grebien F, Niskanen H, Hnisz
            D
  TITLE     Probing condensate microenvironments with a micropeptide killswitch.
  JOURNAL   Nature. 2025 Jun 4. doi: 10.1038/s41586-025-09141-5.
  PUBMED    40468084
REFERENCE   2  (bases 1 to 10916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2025 Jun 4. doi: 10.1038/s41586-025-09141-5."
COMMENT     Sequence Label: pRK5_EGFP-BRD4-NUT
FEATURES             Location/Qualifiers
     source          1..10916
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     548..568
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     594..618
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        828..846
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     828..845
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     859..878
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      933..942
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(939..941,942..944,945..1655)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(939..941,942..944,945..1655)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(984..1005)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1245..1264)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1592..1613
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      7216..7225
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    7229..7363
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7266..7285)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7320..7339
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        7432..7789
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7432..7623
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7455..7475)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7640..7775
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7809..7826)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7809..7825)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7818..7840)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8038..8493
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8125..8144)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8335..8356
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8510..8529)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8629..8651
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8689..8707)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8775..8879
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8880..8948,8949..9740)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9098..9117)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9911..10499
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10400..10419
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10653..10670
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10787..10808
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10823..10828,10829..10846,10847..10853)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10861..10877
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10866..10888
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10885..10901
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10885..10901
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
      721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc tatcgattga attcgtaccg gtgccaccat ggtgagcaag ggcgaggagc
      961 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     1021 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     1081 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     1141 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     1201 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     1261 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     1321 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     1381 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     1441 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     1501 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     1561 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     1621 ccgggatcac tctcggcatg gacgagctgt acaagatgtc tgcggagagc ggccctggga
     1681 cgagattgag aaatctgcca gtaatggggg atggactaga aacttcccaa atgtctacaa
     1741 cacaggccca ggcccaaccc cagccagcca acgcagccag caccaacccc ccgcccccag
     1801 agacctccaa ccctaacaag cccaagaggc agaccaacca actgcaatac ctgctcagag
     1861 tggtgctcaa gacactatgg aaacaccagt ttgcatggcc tttccagcag cctgtggatg
     1921 ccgtcaagct gaacctccct gattactata agatcattaa aacgcctatg gatatgggaa
     1981 caataaagaa gcgcttggaa aacaactatt actggaatgc tcaggaatgt atccaggact
     2041 tcaacactat gtttacaaat tgttacatct acaacaagcc tggagatgac atagtcttaa
     2101 tggcagaagc tctggaaaag ctcttcttgc aaaaaataaa tgagctaccc acagaagaaa
     2161 ccgagatcat gatagtccag gcaaaaggaa gaggacgtgg gaggaaagaa acagggacag
     2221 caaaacctgg cgtttccacg gtaccaaaca caactcaagc atcgactcct ccgcagaccc
     2281 agacccctca gccgaatcct cctcctgtgc aggccacgcc tcaccccttc cctgccgtca
     2341 ccccggacct catcgtccag acccctgtca tgacagtggt gcctccccag ccactgcaga
     2401 cgcccccgcc agtgcccccc cagccacaac ccccacccgc tccagctccc cagcccgtac
     2461 agagccaccc acccatcatc gcggccaccc cacagcctgt gaagacaaag aagggagtga
     2521 agaggaaagc agacaccacc acccccacca ccattgaccc cattcacgag ccaccctcgc
     2581 tgcccccgga gcccaagacc accaagctgg gccagcggcg ggagagcagc cggcctgtga
     2641 aacctccaaa gaaggacgtg cccgactctc agcagcaccc agcaccagag aagagcagca
     2701 aggtctcgga gcagctcaag tgctgcagcg gcatcctcaa ggagatgttt gccaagaagc
     2761 acgccgccta cgcctggccc ttctacaagc ctgtggacgt ggaggcactg ggcctacacg
     2821 actactgtga catcatcaag caccccatgg acatgagcac aatcaagtct aaactggagg
     2881 cccgtgagta ccgtgatgct caggagtttg gtgctgacgt ccgattgatg ttctccaact
     2941 gctataagta caaccctcct gaccatgagg tggtggccat ggcccgcaag ctccaggatg
     3001 tgttcgaaat gcgctttgcc aagatgccgg acgagcctga ggagccagtg gtggccgtgt
     3061 cctccccggc agtgccccct cccaccaagg ttgtggcccc gccctcatcc agcgacagca
     3121 gcagcgatag ctcctcggac agtgacagtt cgactgatga ctctgaggag gagcgagccc
     3181 agcggctggc tgagctccag gagcagctca aagccgtgca cgagcagctt gcagccctct
     3241 ctcagcccca gcagaacaaa ccaaagaaaa aggagaaaga caagaaggaa aagaaaaaag
     3301 aaaagcacaa aaggaaagag gaagtggaag agaataaaaa aagcaaagcc aaggaacctc
     3361 ctcctaaaaa gacgaagaaa aataatagca gcaacagcaa tgtgagcaag aaggagccag
     3421 cgcccatgaa gagcaagccc cctcccacgt atgagtcgga ggaagaggac aagtgcaagc
     3481 ctatgtccta tgaggagaag cggcagctca gcttggacat caacaagctc cccggcgaga
     3541 agctgggccg cgtggtgcat atcatccagt cacgggagcc ctccctgaag aattccaacc
     3601 ccgacgagat tgaaatcgac tttgagaccc tgaagccgtc cacactgcgt gagctggagc
     3661 gctatgtcac ctcctgtttg cggaagaaaa ggaaacctca agctgagaaa gttgatgtga
     3721 ttgccggctc ctccaagatg aagggcttct cgtcctcaga gtcggagagc tccagtgagt
     3781 ccagctcctc tgacagcgaa gactccgaaa cagcatctgc attgccggga ccggatatga
     3841 gcatgaaacc tagtgccgcc ctgtctccat cccctgcact tccctttctc ccaccaactt
     3901 ctgacccacc agaccaccca cccagggagc cacctccaca gcccatcatg ccttcagtat
     3961 tctctccaga caaccctctg atgctctctg ctttccccag ctcactgttg gtgacagggg
     4021 acgggggccc ttgcctcagt ggggctgggg ctggcaaggt cattgtcaaa gtcaagacag
     4081 aaggggggtc agctgagccc tctcaaactc agaactttat ccttactcag actgccctca
     4141 attcgactgc cccgggcact ccctgtggag gccttgaggg tcctgcacct ccatttgtga
     4201 cagcatctaa tgtgaagacc attctgccct ctaaggctgt tggtgtcagc caggagggtc
     4261 ctccaggcct tccgcctcag cctccaccac cagttgctca actggtcccc attgtgcccc
     4321 tggaaaaagc ttggccaggg ccacatggga caaccgggga aggaggtcct gtggccactc
     4381 tatccaagcc ttccctaggt gaccgctcca aaatttccaa ggacgtttat gagaacttcc
     4441 gtcagtggca gcgttacaaa gccttggccc ggaggcacct atcccagagt cctgacacag
     4501 aagctctttc ctgttttctt atcccagtgc ttcgttccct ggcccggctg aagcccacta
     4561 tgaccctgga ggagggactg ccattggctg tgcaggagtg ggagcacacc agcaactttg
     4621 accggatgat cttttatgag atggcagaaa ggttcatgga gtttgaggct gaggagatgc
     4681 agattcagaa cacacagctg atgaatgggt ctcagggcct gtctcctgca acccctttga
     4741 aacttgatcc tctagggccc ctggcctctg aggtttgcca gcagccagtg tacattccga
     4801 agaaggcagc ttctaaaaca agagctcctc gccggcgtca gcgtaaagcc cagagacctc
     4861 ctgctcctga ggcacccaag gagatcccac cagaagctgt gaaggagtat gttgacatca
     4921 tggaatggct ggtggggact cacttggcca ctggggagtc agatggaaaa caagaggaag
     4981 aagggcagca gcaggaggag gaagggatgt atccagatcc aggtctcctg agctacatca
     5041 atgagctgtg ttctcagaag gtctttgtct ccaaggtgga ggctgtcatt caccctcaat
     5101 ttctggcaga tctgctgtcc ccagaaaaac agagagatcc cttggcctta attgaggagc
     5161 tagagcaaga agaaggactc actcttgccc agctggtcca gaagcgactc atggccttgg
     5221 aagaggagga agatgcagag gcgcctccaa gtttcagtgg cgctcagttg gactcaagtc
     5281 cttctggttc tgttgaggat gaagatgggg atgggcggct tcggccctca cctgggcttc
     5341 agggggctgg gggcgccgct tgccttggaa aggtttcttc ttcaggaaaa cgggcaagag
     5401 aagtgcatgg tgggcaggag caagccctag atagccccag agggatgcac agggatggga
     5461 acactctgcc atcccccagc agctgggacc tgcagccaga acttgcagct ccacagggaa
     5521 ctccgggacc cttgggtgtg gagaggagag ggtctgggaa ggttataaac caggtatctc
     5581 tacatcagga tggccatcta ggaggcgctg ggcctcctgg gcactgcctg gtggctgata
     5641 ggacttcaga ggctctgccc ctttgttggc agggaggctt ccagcctgag agcactccca
     5701 gtttggatgc tggacttgca gagctggctc ctctgcaagg acaagggtta gaaaagcaag
     5761 tcctgggatt gcagaaagga caacaaacag ggggtcgtgg agtgcttcct caagggaagg
     5821 agcctttagc agtgccctgg gaaggctctt caggagccat gtggggagat gacagaggta
     5881 cccccatggc tcagagttat gatcagaatc cttcccctag agcagctggg gagagggacg
     5941 atgtctgtct cagcccagga gtttggctga gcagtgagat ggatgctgta ggcttggagc
     6001 tgcctgtaca aatagaggag gtcatagaga gcttccaagt tgagaagtgt gtaactgagt
     6061 atcaggaagg ctgccaggga ctgggctcca ggggcaacat ttccctgggt cctggagaaa
     6121 ccctagtacc tggggatacg gagagcagtg tgattccctg tggaggcaca gttgcggcag
     6181 ctgccctaga aaagagaaac tattgcagct tgccaggacc tttgagggcc aacagcccac
     6241 ccttgaggtc caaagaaaat caagaacaga gctgtgaaac cgtagggcat cccagtgatc
     6301 tgtgggcaga aggctgcttc ccattgctag aaagtggtga ttccacactg gggtcttcca
     6361 aagaaaccct tccacccaca tgccaaggca atctccttat catggggact gaggatgcct
     6421 cctccttgcc tgaagccagt caagaggcag ggagcagagg caattccttt tctcctctgt
     6481 tggaaaccat agaacctgtc aacatactag atgttaaaga tgactgtggc ctccaactaa
     6541 gggtcagcga ggacacctgc ccactgaatg ttcattctta tgacccccaa ggagaaggca
     6601 gggtggatcc tgatctgtcc aagcctaaaa accttgctcc tttacaagag agtcaggagt
     6661 cttacacaac tgggactccc aaagcaacat cttctcacca gggccttgga agcactttgc
     6721 ctagaagggg aaccaggaat gccatagttc cgagagaaac ttctgttagt aaaacacaca
     6781 ggtcagcaga cagggccaaa ggaaaggaga aaaagaaaaa ggaagcagag gaagaggatg
     6841 aggaactctc caactttgct tacctcttgg cctctaaact tagcctctca ccaagggagc
     6901 atcccctcag tcctcaccat gcctcaggag gtcagggcag ccagagagca tcccacctgc
     6961 tccctgctgg agcaaaaggc cccagcaaac ttccatatcc tgttgccaag tctgggaagc
     7021 gagctctagc tggaggtcca gcccctactg aaaagacacc ccactcagga gctcaacttg
     7081 gggtccccag ggagaaaccc ctagctctgg gagtagttcg accctcacag cctcgtaaaa
     7141 ggcggtgtga cagttttgtc acgggcagaa ggaagaaacg acgtcgtagc cagtgagtcg
     7201 acctgcagaa gcttggccgc catggcccaa cttgtttatt gcagcttata atggttacaa
     7261 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     7321 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcgggaatt aattcggcgc
     7381 agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct tctgaggcgg
     7441 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     7501 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     7561 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     7621 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     7681 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     7741 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctgttaac
     7801 agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     7861 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     7921 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
     7981 tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg
     8041 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     8101 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     8161 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     8221 ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat
     8281 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     8341 tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag
     8401 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     8461 cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct
     8521 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     8581 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     8641 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     8701 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     8761 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     8821 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     8881 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     8941 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     9001 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     9061 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     9121 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     9181 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     9241 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     9301 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     9361 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     9421 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     9481 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     9541 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     9601 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     9661 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     9721 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     9781 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     9841 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     9901 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     9961 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    10021 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
    10081 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    10141 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    10201 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    10261 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    10321 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    10381 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    10441 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    10501 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    10561 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    10621 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    10681 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
    10741 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
    10801 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
    10861 ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg aattaa
//
