LOCUS       sequence-474387-        3804 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Minimal integrating shuttle vector depleted of restriction sites 
            outside the polylinker region, yeast selection marker KanMX.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Scutteri L, Barth P, Rahi SJ
  TITLE     Minimal shuttle vectors for Saccharomyces cerevisiae.
  JOURNAL   Synth Biol (Oxf). 2025 May 21;10(1):ysaf010. doi: 
            10.1093/synbio/ysaf010. eCollection 2025.
  PUBMED    40612073
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2025-05-21"; volume: "10(1):ysaf010. doi"; pages: " 
            10.1093/synbio/ysaf010. eCollection 2025"
COMMENT     Sequence Label: pLS400_YCR051W
FEATURES             Location/Qualifiers
     source          1..3804
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..64
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(283..302)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      948..1536
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1437..1456
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1537..1553
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1537..1553
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2431..2447)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            2448..3804
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        2448..2791
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             2792..3601
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      3607..3804
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
       61 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      121 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgcagaag atcagttggg
      181 tgcgcgagtg ggttacatcg aactggacct caacagtggt aagatactag agagttttcg
      241 ccccgaagaa cgattcccaa tgatgtccac ttttaaggtt ctgctatgtg gcgcggtatt
      301 atcccgtatt gacgcggggc aagagcaact aggtcgccgc atacactatt ctcagaatga
      361 cttggttgag tattcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
      421 attatgtagt gctgccataa cgatgagtga taacacggcg gcgaacttac ttctgacaac
      481 gattggagga ccaaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
      541 ccttgatcgt tgggaaccag agctgaatga agccatacca aacgacgagc gtgacaccac
      601 gatgcctgtt gctatggcaa caaccttgcg gaaactatta actggcgaac tacttactct
      661 agcttcccgg caacagttaa tagactggat ggaggctgat aaagttgcag gaccacttct
      721 gcgctctgcg cttccggctg gctggtttat tgctgataaa tctggggccg gagagcgtgg
      781 gtcacgcggt atcatagcag cactagggcc agatggtaag ccctcccgta tcgtagttat
      841 ctacacgacg gggagccagg caactatgga tgaacgaaat agacagatcg ctgagatagg
      901 tgcgtcactg attaagcatt ggtagtagaa aagatcaaag gatcttcttg agatcctttt
      961 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     1021 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     1081 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     1141 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     1201 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     1261 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     1321 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     1381 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     1441 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     1501 ttgtgatgct cgtcaggggg gcggagccta tggaaacagg aaacagctat gacgcgcgct
     1561 aatgtgttgg acaacgacgg cgataccccg ttgcaccatg tggaggatgt ggccactgcc
     1621 aggttgatcg tggaagagct gggtggagac ttcactatca ggaatgtgga gggccaaacg
     1681 ccatacgact cgttcgtcga gaacggtgaa gatggtgagc taatcgagta catgaggatt
     1741 aagtccggcg tggccgatgt tcacggagtg gacggcgtgc agggtgaggg tgtcatcgac
     1801 agcaaattgc tggaagagtt caaggacaac gtgagataca ccttggaaaa tgaccctgag
     1861 gaaggagccg atgaggccac tctgcaacgc aggaggcagt tggaacagat cattacggga
     1921 gacaacgctg aggaggagtt ggaaaggtac atccgtgcta tggtcagaga gcgctccacc
     1981 gtggtcaaag acaggggcaa agagctccta ggtctatata tatatctata tacatattta
     2041 tatatattat tagaacttta caatatagta tataccattc attgtttaag tttcgggtaa
     2101 tacttttttt ttccttgtca taaccccaaa aattttcgat gcctttgata taattgagaa
     2161 caagaagagt ttgcaggtga caaaaatcga tgattatagg tgttgtgacg acaaaatgaa
     2221 cgctaatata tgggtggctg cttcagatgg taatttggac cgagtggaac atatcctccg
     2281 cgagagtaaa ggcgccatga ccccgcaatc caaggacatt aacggctaca ctccaatgca
     2341 tgctgccgcc gcatacggcc acctggattt gctgaagaaa atgtgcaatg agtacaatgg
     2401 agacatgtcg acctgcaggc atgccctagg actggccgtc gttttacgac atggaggccc
     2461 agaataccct ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt gactgtcgcc
     2521 cgtacattta gcccatacat ccccatgtat aatcatttgc atccatacat tttgatggcc
     2581 gcacggcgcg aagcaaaaat tacggctcct cgctgcagac ctgcgagcag ggaaacgctc
     2641 ccctcacaga cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt
     2701 aggatttgcc actgaggttc ttctttcata tacttccttt taaaatcttg ctaggataca
     2761 gttctcacat cacatccgaa cataaacaac catgggtaag gaaaagactc acgtttcgag
     2821 gccgcgatta aactccaata tggatgctga tttatatggg tataaatggg ctagggataa
     2881 tgtcgggcaa tcaggtgcga caatctaccg attgtatggg aagcccgatg cgccagagtt
     2941 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
     3001 aaactggctg acggagttta tgcctctacc gaccatcaag cattttatcc gtactcctga
     3061 tgacgcatgg ttgctcacca cagcgatccc cggcaaaaca gctttccagg tattagaaga
     3121 atatccagat tcaggtgaaa acattgttga tgcgctggct gtgttcctgc gtcggttgca
     3181 ctcgattcct gtttgtaatt gtccttttaa cagtgatcgc gtatttcgac tcgcacaggc
     3241 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
     3301 ctggcctgtt gaacaagtct ggaaagaaat gcacaaactt ttgccattct caccagattc
     3361 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattgat
     3421 aggttgtatt gatgtaggac gagtaggaat cgcagaccgc taccaagacc ttgcaatcct
     3481 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
     3541 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
     3601 atcaatactg acaataaaaa gattcttgtt ttcaagaact tgtcatttgt atagtttttt
     3661 tatattgtag ttgttctatt ttaatcaaat gttagcgtga tttatatttt ttttcgcctc
     3721 gacatcatct gcccagatgc gaagttaagt gcgcagaaag taatatcatg cgtcaatcgt
     3781 atgtgaatga tggtcgctat actg
//
