LOCUS       sequence-474394-       12641 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For overexpression of mCherry-P300.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12641)
  AUTHORS   Zhang Y, Stoppelkamp I, Fernandez-Pernas P, Allram M, Charman M, 
            Magalhaes AP, Piedavent-Salomon M, Sommer G, Sung YC, Meyer K, Grams
            N, Halko E, Dongre S, Meierhofer D, Malszycki M, Ilik IA, Aktas T, 
            Kraushar ML, Vastenhouw N, Weitzman MD, Grebien F, Niskanen H, Hnisz
            D
  TITLE     Probing condensate microenvironments with a micropeptide killswitch.
  JOURNAL   Nature. 2025 Jun 4. doi: 10.1038/s41586-025-09141-5.
  PUBMED    40468084
REFERENCE   2  (bases 1 to 12641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2025 Jun 4. doi: 10.1038/s41586-025-09141-5."
COMMENT     Sequence Label: pRK5_mCherry-P300
FEATURES             Location/Qualifiers
     source          1..12641
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     548..568
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     594..618
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        828..846
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     828..845
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     859..878
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      933..942
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4818..6668
                     /codon_start=1
                     /gene="EP300"
                     /product="catalytic histone acetyltransferase core of the 
                     human E1A-associated protein p300 (Delvecchio et al., 
                     2013)"
                     /label=p300 core
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     regulatory      6432..6441
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8941..8950
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    8954..9088
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8991..9010)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9045..9064
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        9157..9514
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9157..9348
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9180..9200)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9365..9500
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9534..9551)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9534..9550)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9543..9565)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9763..10218
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9850..9869)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10060..10081
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10235..10254)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10354..10376
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10414..10432)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10500..10604
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10605..10673,10674..11465)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10823..10842)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11636..12224
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12125..12144
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12378..12395
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12512..12533
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12548..12553,12554..12571,12572..12578)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12586..12602
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12591..12613
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12610..12626
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12610..12626
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
      721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc tatcgattga attcgtaccg gtgccaccat ggtttccaaa ggagaggagg
      961 ataatatggc tataattaaa gagtttatgc ggttcaaggt gcatatggag ggatctgtga
     1021 acggtcacga gttcgaaatc gagggcgaag gcgaggggcg accctatgaa gggacacaga
     1081 cggccaaact gaaggtgacc aagggtggcc ccctgccttt cgcctgggac atcctgtcac
     1141 cccagtttat gtacggctct aaggcttacg ttaagcaccc tgccgatatt ccggactact
     1201 tgaaactgag cttccctgaa gggttcaagt gggaacgggt aatgaatttt gaggatggcg
     1261 gtgtagtcac agttacccag gacagctctc ttcaggacgg agaatttatc tataaggtta
     1321 aacttcgggg cactaacttc ccatcagacg gccccgtgat gcagaagaaa actatgggct
     1381 gggaagccag ctcagaacgc atgtatcccg aggacggagc actgaaggga gaaatcaagc
     1441 agcgactcaa gctgaaggat gggggacatt atgatgctga ggtcaaaacc acctataagg
     1501 ccaagaaacc cgttcagctt cctggggcat acaacgtgaa tatcaaactg gatattacat
     1561 cccataacga agactatacc atcgtggaac agtacgagcg ggccgagggc agacatagca
     1621 cagggggcat ggatgaattg tacaaggggg gaggcggcag tggcggaggg ggatctatgg
     1681 ccgagaatgt ggtggaaccg gggccgcctt cagccaagcg gcctaaactc tcatctccgg
     1741 ccctctcggc gtccgccagc gatggcacag attttggctc tctatttgac ttggagcacg
     1801 acttaccaga tgaattaatc aactctacag aattgggact aaccaatggt ggtgatatta
     1861 atcagcttca gacaagtctt ggcatggtac aagatgcagc ttctaaacat aaacagctgt
     1921 cagaattgct gcgatctggt agttccccta acctcaatat gggagttggt ggcccaggtc
     1981 aagtcatggc cagccaggcc caacagagca gtcctggatt aggtttgata aatagcatgg
     2041 tcaaaagccc aatgacacag gcaggcttga cttctcccaa catggggatg ggcactagtg
     2101 gaccaaatca gggtcctacg cagtcaacag gtatgatgaa cagtccagta aatcagcctg
     2161 ccatgggaat gaacacaggg atgaatgcgg gcatgaatcc tggaatgttg gctgcaggca
     2221 atggacaagg gataatgcct aatcaagtca tgaacggttc aattggagca ggccgagggc
     2281 gacagaatat gcagtaccca aacccaggca tgggaagtgc tggcaactta ctgactgagc
     2341 ctcttcagca gggctctccc cagatgggag gacaaacagg attgagaggc ccccagcctc
     2401 ttaagatggg aatgatgaac aaccccaatc cttatggttc accatatact cagaatcctg
     2461 gacagcagat tggagccagt ggccttggtc tccagattca gacaaaaact gtactatcaa
     2521 ataacttatc tccatttgct atggacaaaa aggcagttcc tggtggagga atgcccaaca
     2581 tgggtcaaca gccagccccg caggtccagc agccaggcct ggtgactcca gttgcccaag
     2641 ggatgggttc tggagcacat acagctgatc cagagaagcg caagctcatc cagcagcagc
     2701 ttgttctcct tttgcatgct cacaagtgcc agcgccggga acaggccaat ggggaagtga
     2761 ggcagtgcaa ccttccccac tgtcgcacaa tgaagaatgt cctaaaccac atgacacact
     2821 gccagtcagg caagtcttgc caagtggcac actgtgcatc ttctcgacaa atcatttcac
     2881 actggaagaa ttgtacaaga catgattgtc ctgtgtgtct ccccctcaaa aatgctggtg
     2941 ataagagaaa tcaacagcca attttgactg gagcacccgt tggacttgga aatcctagct
     3001 ctctaggggt gggtcaacag tctgccccca acctaagcac tgttagtcag attgatccca
     3061 gctccataga aagagcctat gcagctcttg gactacccta tcaagtaaat cagatgccga
     3121 cacaacccca ggtgcaagca aagaaccagc agaatcagca gcctgggcag tctccccaag
     3181 gcatgcggcc catgagcaac atgagtgcta gtcctatggg agtaaatgga ggtgtaggag
     3241 ttcaaacgcc gagtcttctt tctgactcaa tgttgcattc agccataaat tctcaaaacc
     3301 caatgatgag tgaaaatgcc agtgtgccct ccctgggtcc tatgccaaca gcagctcaac
     3361 catccactac tggaattcgg aaacagtggc acgaagatat tactcaggat cttcgaaatc
     3421 atcttgttca caaactcgtc caagccatat ttcctacgcc ggatcctgct gctttaaaag
     3481 acagacggat ggaaaaccta gttgcatatg ctcggaaagt tgaaggggac atgtatgaat
     3541 ctgcaaacaa tcgagcggaa tactaccacc ttctagctga gaaaatctat aagatccaga
     3601 aagaactaga agaaaaacga aggaccagac tacagaagca gaacatgcta ccaaatgctg
     3661 caggcatggt tccagtttcc atgaatccag ggcctaacat gggacagccg caaccaggaa
     3721 tgacttctaa tggccctcta cctgacccaa gtatgatccg tggcagtgtg ccaaaccaga
     3781 tgatgcctcg aataactcca caatctggtt tgaatcaatt tggccagatg agcatggccc
     3841 agccccctat tgtaccccgg caaacccctc ctcttcagca ccatggacag ttggctcaac
     3901 ctggagctct caacccgcct atgggctatg ggcctcgtat gcaacagcct tccaaccagg
     3961 gccagttcct tcctcagact cagttcccat cacagggaat gaatgtaaca aatatccctt
     4021 tggctccgtc cagcggtcaa gctccagtgt ctcaagcaca aatgtctagt tcttcctgcc
     4081 cggtgaactc tcctataatg cctccagggt ctcaggggag ccacattcac tgtccccagc
     4141 ttcctcaacc agctcttcat cagaattcac cctcgcctgt acctagtcgt acccccaccc
     4201 ctcaccatac tcccccaagc ataggggctc agcagccacc agcaacaaca attccagccc
     4261 ctgttcctac acctcctgcc atgccacctg ggccacagtc ccaggctcta catccccctc
     4321 caaggcagac acctacacca ccaacaacac aacttcccca acaagtgcag ccttcacttc
     4381 ctgctgcacc ttctgctgac cagccccagc agcagcctcg ctcacagcag agcacagcag
     4441 cgtctgttcc taccccaaca gcaccgctgc ttcctccgca gcctgcaact ccactttccc
     4501 agccagctgt aagcattgaa ggacaggtat caaatcctcc atctactagt agcacagaag
     4561 tgaattctca ggccattgct gagaagcagc cttcccagga agtgaagatg gaggccaaaa
     4621 tggaagtgga tcaaccagaa ccagcagata ctcagccgga ggatgtttca gagtctaaag
     4681 tggaagactg taaaatggaa tctaccgaaa cagaagagag aagcactgag ttaaaaactg
     4741 aaataaaaga ggaggaagac cagccaagta cttcagctac ccagtcatct ccggctccag
     4801 gacagtcaaa gaaaaagatt ttcaaaccag aagaactacg acaggcactg atgccaacat
     4861 tggaggcact ttaccgtcag gatccagaat cccttccctt tcgtcaacct gtggaccctc
     4921 agcttttagg aatccctgat tactttgata ttgtgaagag ccccatggat ctttctacca
     4981 ttaagaggaa gttagacact ggacagtatc aggagccctg gcagtatgtc gatgatattt
     5041 ggcttatgtt caataatgcc tggttatata accggaaaac atcacgggta tacaaatact
     5101 gctccaagct ctctgaggtc tttgaacaag aaattgaccc agtgatgcaa agccttggat
     5161 actgttgtgg cagaaagttg gagttctctc cacagacact gtgttgctac ggcaaacagt
     5221 tgtgcacaat acctcgtgat gccacttatt acagttacca gaacaggtat catttctgtg
     5281 agaagtgttt caatgagatc caaggggaga gcgtttcttt gggggatgac ccttcccagc
     5341 ctcaaactac aataaataaa gaacaatttt ccaagagaaa aaatgacaca ctggatcctg
     5401 aactgtttgt tgaatgtaca gagtgcggaa gaaagatgca tcagatctgt gtccttcacc
     5461 atgagatcat ctggcctgct ggattcgtct gtgatggctg tttaaagaaa agtgcacgaa
     5521 ctaggaaaga aaataagttt tctgctaaaa ggttgccatc taccagactt ggcacctttc
     5581 tagagaatcg tgtgaatgac tttctgaggc gacagaatca ccctgagtca ggagaggtca
     5641 ctgttagagt agttcatgct tctgacaaaa ccgtggaagt aaaaccaggc atgaaagcaa
     5701 ggtttgtgga cagtggagag atggcagaat cctttccata ccgaaccaaa gccctctttg
     5761 cctttgaaga aattgatggt gttgacctgt gcttctttgg catgcatgtt caagagtatg
     5821 gctctgactg ccctccaccc aaccagagga gagtatacat atcttacctc gatagtgttc
     5881 atttcttccg tcctaaatgc ttgaggactg cagtctatca tgaaatccta attggatatt
     5941 tagaatatgt caagaaatta ggttacacaa cagggcatat ttgggcatgt ccaccaagtg
     6001 agggagatga ttatatcttc cattgccatc ctcctgacca gaagataccc aagcccaagc
     6061 gactgcagga atggtacaaa aaaatgcttg acaaggctgt atcagagcgt attgtccatg
     6121 actacaagga tatttttaaa caagctactg aagatagatt aacaagtgca aaggaattgc
     6181 cttatttcga gggtgatttc tggcccaatg ttctggaaga aagcattaag gaactggaac
     6241 aggaggaaga agagagaaaa cgagaggaaa acaccagcaa tgaaagcaca gatgtgacca
     6301 agggagacag caaaaatgct aaaaagaaga ataataagaa aaccagcaaa aataagagca
     6361 gcctgagtag gggcaacaag aagaaacccg ggatgcccaa tgtatctaac gacctctcac
     6421 agaaactata tgccaccatg gagaagcata aagaggtctt ctttgtgatc cgcctcattg
     6481 ctggccctgc tgccaactcc ctgcctccca ttgttgatcc tgatcctctc atcccctgcg
     6541 atctgatgga tggtcgggat gcgtttctca cgctggcaag ggacaagcac ctggagttct
     6601 cttcactccg aagagcccag tggtccacca tgtgcatgct ggtggagctg cacacgcaga
     6661 gccaggaccg ctttgtctac acctgcaatg aatgcaagca ccatgtggag acacgctggc
     6721 actgtactgt ctgtgaggat tatgacttgt gtatcacctg ctataacact aaaaaccatg
     6781 accacaaaat ggagaaacta ggccttggct tagatgatga gagcaacaac cagcaggctg
     6841 cagccaccca gagcccaggc gattctcgcc gcctgagtat ccagcgctgc atccagtctc
     6901 tggtccatgc ttgccagtgt cggaatgcca attgctcact gccatcctgc cagaagatga
     6961 agcgggttgt gcagcatacc aagggttgca aacggaaaac caatggcggg tgccccatct
     7021 gcaagcagct cattgccctc tgctgctacc atgccaagca ctgccaggag aacaaatgcc
     7081 cggtgccgtt ctgcctaaac atcaagcaga agctccggca gcaacagctg cagcaccgac
     7141 tacagcaggc ccaaatgctt cgcaggagga tggccagcat gcagcggact ggtgtggttg
     7201 ggcagcaaca gggcctccct tcccccactc ctgccactcc aacgacacca actggccaac
     7261 agccaaccac cccgcagacg ccccagccca cttctcagcc tcagcctacc cctcccaata
     7321 gcatgccacc ctacttgccc aggactcaag ctgctggccc tgtgtcccag ggtaaggcag
     7381 caggccaggt gacccctcca acccctcctc agactgctca gccacccctt ccagggcccc
     7441 cacctgcagc agtggaaatg gcaatgcaga ttcagagagc agcggagacg cagcgccaga
     7501 tggcccacgt gcaaattttt caaaggccaa tccaacacca gatgcccccg atgactccca
     7561 tggcccccat gggtatgaac ccacctccca tgaccagagg tcccagtggg catttggagc
     7621 cagggatggg accgacaggg atgcagcaac agccaccctg gagccaagga ggattgcctc
     7681 agccccagca actacagtct gggatgccaa ggccagccat gatgtcagtg gcccagcatg
     7741 gtcaaccttt gaacatggct ccacaaccag gattgggcca ggtaggtatc agcccactca
     7801 aaccaggcac tgtgtctcaa caagccttac aaaacctttt gcggactctc aggtctccca
     7861 gctctcccct gcagcagcaa caggtgctta gtatccttca cgccaacccc cagctgttgg
     7921 ctgcattcat caagcagcgg gctgccaagt atgccaactc taatccacaa cccatccctg
     7981 ggcagcctgg catgccccag gggcagccag ggctacagcc acctaccatg ccaggtcagc
     8041 agggggtcca ctccaatcca gccatgcaga acatgaatcc aatgcaggcg ggcgttcaga
     8101 gggctggcct gccccagcag caaccacagc agcaactcca gccacccatg ggagggatga
     8161 gcccccaggc tcagcagatg aacatgaacc acaacaccat gccttcacaa ttccgagaca
     8221 tcttgagacg acagcaaatg atgcaacagc agcagcaaca gggagcaggg ccaggaatag
     8281 gccctggaat ggccaaccat aaccagttcc agcaacccca aggagttggc tacccaccac
     8341 agcagcagca gcggatgcag catcacatgc aacagatgca acaaggaaat atgggacaga
     8401 taggccagct tccccaggcc ttgggagcag aggcaggtgc cagtctacag gcctatcagc
     8461 agcgactcct tcagcaacag atggggtccc ctgttcagcc caaccccatg agcccccagc
     8521 agcatatgct cccaaatcag gcccagtccc cacacctaca aggccagcag atccctaatt
     8581 ctctctccaa tcaagtgcgc tctccccagc ctgtcccttc tccacggcca cagtcccagc
     8641 ccccccactc cagtccttcc ccaaggatgc agcctcagcc ttctccacac cacgtttccc
     8701 cacagacaag ttccccacat cctggactgg tagctgccca ggccaacccc atggaacaag
     8761 ggcattttgc cagcccggac cagaattcaa tgctttctca gcttgctagc aatccaggca
     8821 tggcaaacct ccatggtgca agcgccacgg acctgggact cagcaccgat aactcagact
     8881 tgaattcaaa cctctcacag agtacactag acatacacta ggtcgacctg cagaagcttg
     8941 gccgccatgg cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     9001 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     9061 catcaatgta tcttatcatg tctggatcgg gaattaattc ggcgcagcac catggcctga
     9121 aataacctct gaaagaggaa cttggttagg taccttctga ggcggaaaga accagctgtg
     9181 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     9241 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     9301 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     9361 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     9421 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     9481 aggaggcttt tttggaggcc taggcttttg caaaaagctg ttaacagctt ggcactggcc
     9541 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     9601 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     9661 caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat
     9721 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
     9781 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     9841 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     9901 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     9961 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
    10021 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
    10081 gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
    10141 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
    10201 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
    10261 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
    10321 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
    10381 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
    10441 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
    10501 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
    10561 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
    10621 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
    10681 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
    10741 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
    10801 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
    10861 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
    10921 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
    10981 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
    11041 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
    11101 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
    11161 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
    11221 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
    11281 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
    11341 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
    11401 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
    11461 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    11521 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    11581 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    11641 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    11701 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    11761 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
    11821 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    11881 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    11941 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    12001 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    12061 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    12121 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    12181 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    12241 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
    12301 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
    12361 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
    12421 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
    12481 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
    12541 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
    12601 aatttcacac aggaaacagc tatgacatga ttacgaatta a
//
