LOCUS       sequence-474400-       14764 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14764)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 14764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1607
FEATURES             Location/Qualifiers
     source          1..14764
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6056..6070
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     misc_feature    10621..11209
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10674..10694)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11092..11103)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             11281..11514
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11592..11713
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11629..11648)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11683..11702
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11753..11888
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11908..11927)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11909..11927)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11937..11954)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11937..11953)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11946..11968)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      12095..12550
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12182..12201)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12392..12413
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        12576..12680
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12681..12749,12750..13541)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12899..12918)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13712..14300
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14201..14220
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14454..14471
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    14588..14609
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(14624..14629,14630..14647,14648..14654)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14662..14678
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14667..14689
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14686..14702
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14686..14702
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     14721..14741
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        14723..14741
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggag tctggagaac ggttaccatc
     4141 ctcagcagcc tcctctacta caccaacttc atcttcgaca ccttctgtgg cttcagtagt
     4201 ttcaaaaggt ggcctttcca ctggagttgc ttcacttagc tctacaatca acccatgtgg
     4261 acatttattc agaacagctg gggatcaacc gtttaacctg tccacagtgt cgagtgcctt
     4321 cccaatggtc agccacccag tctttggtct acattcagcc agctcagggc attcagaatt
     4381 tggtggtttg gggacacttg gtacacccac agccttagcc gcacatcccc aactagcatc
     4441 ttttccagaa tggtggcgaa caactgatgc tcatactcgt acaggagcaa ccttctttcc
     4501 accattactg ggaattccac cactatttgc tcccccagcc cagaatcatg attcttcttc
     4561 attccattca aggacttcgg gaaaaagtaa tcgaaatggt cccgaaaaag gtgtaaatgg
     4621 gtcaataaat ggaagtaata catcatctgt aattggtatc aacacatctg tactatccac
     4681 tactgcttca agttccatgg gacaaactaa aagtacaagc tcaggtggag gaaatcgaaa
     4741 atgtaatcag gaacaaagca aaaaccagcc tttggatgct agagttgaca aaatcaaaga
     4801 taagaaacca aggaagaaag caatggaaag ttctagcaac agtgatagtg attcaggcac
     4861 atcatcagac acctcaagtg aaggcattag tagcagtgat tcagatgatc tagaagaaga
     4921 tgaagaagaa gaagatcaaa gtattgaaga aagtgaagat gatgattctg attcagagag
     4981 tgaagcacaa cataaaagta acaaccaggt gctattacat ggtatttcag acccaaaagc
     5041 agatggacag aaagcaactg aaaaagccca ggaaaaaaga atacaccagc cattacctct
     5101 tgcgtctgaa tcccagactc actcattcca atcccagcag aagcagcctc aggttttgtc
     5161 acagcagctt ccatttattt tccaaagctc tcaggcaaag gaggaatctg tgaacaaaca
     5221 caccagtgta atacagtcta cgggattggt gtccaatgtg aaacctttat ctttggtaaa
     5281 tcaagccaaa aaggaaactt acatgaaact catagttcct tctcctgatg ttcttaaagc
     5341 agggaataaa aatacctctg aagaatctag tttattgacc agtgaattga gatccaaacg
     5401 ggaacaatat aaacaggcat tcccatcaca gttaaagaaa caagagtcat cgaagagcct
     5461 gaagaaggtt attgcagctt tgtcaaatcc aaaagcaacc tctagttcac cagcacatcc
     5521 aaaacaaaca ttagaaaaca accacccaaa tccattcttg acaaatgcac ttttaggtaa
     5581 tcaccaacca aatggagtta ttcaaagtgt cattcaagaa gctcctctag cacttactac
     5641 caaaactaaa atgcagagca agattaatga aaacattgct gctgcaagta gcaccccttt
     5701 ttcctcacct gtaaatctga gtacaagtgg gagaagaacc cctggcaatc agacacctgt
     5761 aatgccctct gcctctccca tcctgcatag tcaagggaag gaaaaagcag ttagcaataa
     5821 tgtaaaccca gtaaaaacac agcatcactc ccatcctgca aaatctttag tggaacaatt
     5881 cagaggaaca gattcagaca ttcccagtag taaagattct gaagattcaa atgaggatga
     5941 agaggaagat gatgaagaag aagatgagga agatgatgaa gatgatgaat ctgatgacag
     6001 ccaatcagaa tcagatagta attcagaatc agatacagaa ggatcagaag aagaagatga
     6061 tgatgataaa gaccaagatg aatcagatag tgatactgaa ggagagaaaa cttcaatgaa
     6121 actgaataaa acaacttcct ctgtcaaaag cccttccatg agtctcacag gtcactcaac
     6181 acctcgtaac ctccacatag caaaagcccc aggctctgct cctgctgcct tatgttctga
     6241 atcccagtca cctgcttttc ttggtacatc ttcttccaca cttacttcaa gcccacactc
     6301 tggcacttcc aaaagaagaa gagtaacaga tgaacgtgaa ctgcgtattc cattggaata
     6361 tggctggcag agagagacaa gaataagaaa ctttggaggg cgccttcaag gagaagtagc
     6421 atattatgct ccatgtggaa agaaacttag gcagtaccct gaagtaataa agggaatgca
     6481 gtggtgtctt ttgaaagaag aggatgtcat tcctcgtatc agggcaatgg aaggtcgtag
     6541 aggaagacca ccaaatccag atagacaacg agcaagagag gaatccagga tgagacgtcg
     6601 gaaaggtcga cctccaaatg ttggcaatgc tgaattccta gataacgcag atgcaaagtt
     6661 gctaagaaaa ctgcaagctc aagaaatagc caggcaagca gcacaaataa agcttttgag
     6721 aaaacttcaa aagcaggaac aggctcgggt tgctaaagaa gccaaaaaac aacaagcaat
     6781 aatggctgct gaggagaagc ggaagcaaaa agaacagata aagattatga aacagcagga
     6841 aaaaattaag agaatacagc aaatcagaat ggaaaaagaa cttcgagctc agcaaattct
     6901 agaggctaaa aagaaaaaga aggaagaagc ggcaaatgcc aaattattgg aggccgagaa
     6961 acgaataaag gaaaaagaaa tgagaagaca acaagctgtt cttctgaaac atcaggaacg
     7021 agagcgaagg cgacaacaca tgatgcttat gaaagctatg gaagctcgta aaaaagcaga
     7081 agaaaaagag cggttgaaac aagaaaaacg tgatgagaaa agattaaata aagagcgtaa
     7141 actagagcag cgaagattag aattagaaat ggcaaaggaa ctaaagaagc ctaatgaaga
     7201 catgtgctta gcagaccaaa agcctttgcc agagttgcct cgtattccag gacttgttct
     7261 ctctggaagt acattttcag actgtctcat ggtggtgcag ttcttacgaa actttggtaa
     7321 agttttgggc tttgatgtga atattgatgt tccaaacctg agtgttcttc aagagggatt
     7381 gctaaatata ggggacagca tgggtgaagt acaagacttg cttgtgaggc tcctctcagc
     7441 tgctgtatgt gatccaggtc taataacagg atacaaggct aaaacagctc ttggagaaca
     7501 tttgctgaat gttggtgtga atcgagacaa tgtttccgag attttacaga tatttatgga
     7561 agcccactgt ggacaaactg agcttactga aagtctgaag accaaagctt ttcaggctca
     7621 cactccagca cagaaagctt cagtcctggc tttcctgatc aatgaactgg catgcagcaa
     7681 gagtgtggtc agtgaaatcg acaagaacat tgattatatg tcaaacttga ggagagataa
     7741 atgggtggta gaaggtaaac tccgcaagct cagaatcatt catgctaaga aaacaggcaa
     7801 aagagacact tcaggtggca ttgatctggg agaagagcag catcccttgg gcacacccac
     7861 tccaggacgc aagcgaagaa ggaagggagg agacagtgat tatgacgatg atgatgacga
     7921 tgacagtgat gaccaagggg atgaagatga tgaggatgaa gaagataaag aagacaaaaa
     7981 aggaaaaaag actgatatct gtgaagatga ggatgaaggt gaccaagcag caagtgttga
     8041 agagctggaa aaacagattg aaaaactgag taaacaacag agtcagtaca gaaggaagct
     8101 ctttgatgcg tctcactcat tgcgttcagt gatgtttggc caagatcgtt acagacgccg
     8161 gtactggatt cttccccaat gtggggggat ttttgtagaa ggcatggaga gtggtgaagg
     8221 actagaagaa attgcaaaag aaagagaaaa actgaaaaag gcagaaagtg tccagatcaa
     8281 agaagaaatg tttgagactt ctggggacag tttaaattgt tcaaatacag atcactgtga
     8341 acaaaaggaa gatcttaaag aaaaagataa cacaaatcta ttccttcaga aacctggctc
     8401 tttttccaaa ttaagcaagc ttttggaagt agctaagatg cctcctgagt cagaggttat
     8461 gacccccaaa ccaaatgctg gtgcaaatgg gtgcacgttg tcttatcaga acagtggaaa
     8521 acattcactg ggcagcgttc agtcaacagc aacgcaaagc aatgtggaaa aggcagactc
     8581 taataatctg tttaatactg gttcaagtgg tccagggaag ttctacagtc ctctccccaa
     8641 tgaccagtta ctaaaaacgc tgactgaaaa gaatagacaa tggtttagtc ttttgccacg
     8701 aacaccctgt gatgacactt cacttactca tgccgatatg tcaactgctt ctttggtgac
     8761 tcctcagtct cagccaccat ctaagtcacc ttcacctacc ccagctcctc ttggatcttc
     8821 tgctcagaat cctgttggct taaatccatt tgctttatca cctcttcagg tgaaaggtgg
     8881 agtatctatg atgggacttc agttttgtgg atggcccact ggtgtggtta cttctaatat
     8941 tccatttaca tcatctgtac ctagtctagg atcggggtta gggttatcag aaggaaatgg
     9001 taattcattc ttgacttcca atgttgcttc aagtaaaagt gaatctccag taccacagaa
     9061 tgaaaaggcc acttcagctc aacctgcagc tgttgaagta gcaaaaccag tagattttcc
     9121 tagtccaaaa cctattccag aagaaatgca gtttggttgg tggagaatta ttgacccaga
     9181 ggacctaaaa gctttgctca aagtgctgca tctcagagga ataagagaaa aggcattaca
     9241 aaaacaaatt cagaaacatt tggattatat tactcaagcc tgcctcaaga ataaggatgt
     9301 tgctattatt gaactgaatg aaaatgaaga aaaccaagta actcgagata ttgtggagaa
     9361 ctggtcagta gaagaacaag caatggaaat ggatttgagt gtccttcaac aggtagaaga
     9421 tctagaaagg agagttgcat cagcaagttt gcaagtgaag ggttggatgt gtccagagcc
     9481 tgcatcagaa agggaggact tggtatattt tgaacataaa tcatttacta aattgtgcaa
     9541 ggagcatgat ggagaattta ctggcgaaga cgaaagcagt gcacatgcac tagaacggaa
     9601 gagtgacaac cccctagata tagctgtaac caggctggct gatttggagc ggaacattga
     9661 aagaagaatt gaagaggata ttgctccagg gctcagggta tggagaaggg cattatcaga
     9721 agctcgcagt gctgcacagg tagctctgtg cattcagcaa ttacagaaat caatagcatg
     9781 ggaaaaatca attatgaaag tttactgcca aatctgtcga aagggagata atgaagaact
     9841 gcttcttctt tgtgatggct gtgacaaagg ctgtcatacc tactgccata gacccaagat
     9901 tacaacaatc ccagatggag actggttttg tccagcttgc attgctaagg caagtggtca
     9961 aactctaaaa atcaaaaaac ttcatgtcaa aggaaaaaag actaatgagt caaagaaagg
    10021 caagaaggta actttaacag gagatactga agatgaagac tctgcatcta caagtagttc
    10081 actaaaaaga ggaaacaaag acctcaagaa aagaaaaatg gaggaaaaca cttctattaa
    10141 cttgtcaaaa caagaaagtt ttacttcagt taagaaacct aaaagagatg actccaagga
    10201 cctagctctt tgcagtatga ttctgactga aatggaaact catgaggatg catggccttt
    10261 tctacttcct gtaaacttga aacttgttcc tggttataag aaagttatta agaagcctat
    10321 ggatttttcc acaattagag agaaactaag tagtggacag tatccaaacc ttgaaacctt
    10381 tgctctagat gtcaggcttg tttttgacaa ctgtgaaaca tttaatgaag atgattctga
    10441 tataggcaga gctggccaca atatgaggaa gtattttgaa aaaaagtgga cagatacttt
    10501 caaagtgagc tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta
    10561 tatatcttgt ggaaaggacg aaaggttcgc gatccacagg aggtcacgcg ttaagtcgac
    10621 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
    10681 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
    10741 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
    10801 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
    10861 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
    10921 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
    10981 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
    11041 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
    11101 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
    11161 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
    11221 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
    11281 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
    11341 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
    11401 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
    11461 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt
    11521 atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
    11581 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
    11641 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    11701 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
    11761 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
    11821 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
    11881 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
    11941 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
    12001 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
    12061 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
    12121 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
    12181 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
    12241 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
    12301 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
    12361 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
    12421 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
    12481 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
    12541 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
    12601 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
    12661 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
    12721 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
    12781 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
    12841 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
    12901 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
    12961 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
    13021 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
    13081 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
    13141 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
    13201 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
    13261 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
    13321 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
    13381 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
    13441 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
    13501 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
    13561 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
    13621 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
    13681 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
    13741 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
    13801 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
    13861 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
    13921 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
    13981 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
    14041 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
    14101 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
    14161 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
    14221 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
    14281 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
    14341 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
    14401 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
    14461 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
    14521 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
    14581 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
    14641 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
    14701 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
    14761 aagc
//
