LOCUS       sequence-474419-        5676 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV construct used as a cell filler to label neurons with tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5676)
  TITLE     AAV-constructs used for labeling and synapse inactivation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-hSyn-tdTomato
FEATURES             Location/Qualifiers
     source          1..5676
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     21..40
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(259..363)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(389..844)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(526..547)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     738..757
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   916..1045
                     /label=AAV2 ITR
     promoter        1127..1574
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      1602..1611
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3051..3639
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3104..3124)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3522..3533)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3642..3658)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3643..3659)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    3688..3809
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3699..3718)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3753..3772
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(4315..4903)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4395..4414)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5074..5676,1..189,190..258))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
       61 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
      121 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
      181 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
      241 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
      301 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
      361 gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc
      421 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
      481 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
      541 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
      601 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
      661 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
      721 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
      781 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
      841 gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
      901 tcgctattac gccagctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg
      961 cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg
     1021 gccaactcca tcactagggg ttccttgtag ttaatgatta acccgccatg ctacttatct
     1081 acgtagccat gctctaggaa gatctctgca gagggccctg cgtatgagtg caagtgggtt
     1141 ttaggaccag gatgaggcgg ggtgggggtg cctacctgac gaccgacccc gacccactgg
     1201 acaagcaccc aacccccatt ccccaaattg cgcatcccct atcagagagg gggaggggaa
     1261 acaggatgcg gcgaggcgcg tgcgcactgc cagcttcagc accgcggaca gtgccttcgc
     1321 ccccgcctgg cggcgcgcgc caccgccgcc tcagcactga aggcgcgctg acgtcactcg
     1381 ccggtccccc gcaaactccc cttcccggcc accttggtcg cgtccgcgcc gccgccggcc
     1441 cagccggacc gcaccacgcg aggcgcgaga taggggggca cgggcgcgac catctgcgct
     1501 gcggcgccgg cgactcagcg ctgcctcagt ctgcggtggg cagcggagga gtcgtgtcgt
     1561 gcctgagagc gcagtcgaat tcaagctgct agcaaggatc cgccaccatg gttagcaagg
     1621 gtgaagaggt gattaaagag tttatgcgtt ttaaagtacg catggagggt agcatgaacg
     1681 gtcatgagtt tgagatcgag ggcgaaggcg aaggtcgtcc gtacgagggt acgcagaccg
     1741 ctaaactgaa agtaaccaaa ggcggtcctc tgccgttcgc gtgggacatc ctgtccccgc
     1801 agtttatgta tggttccaag gcctacgtca aacacccagc cgatatcccg gactacaaga
     1861 agctgtcttt cccggagggt ttcaagtggg agcgtgtaat gaactttgag gacggtggcc
     1921 tggttaccgt cacccaggat agctctctgc aagatggcac cctgatctac aaggtgaaga
     1981 tgcgtggtac gaactttccg ccggatggcc cggtgatgca gaaaaagacg atgggatggg
     2041 aagcatctac cgagcgcctg tatccgcgtg acggtgttct gaaaggtgag atccaccagg
     2101 cgctgaaact gaaggacggc ggtcactacc tggttgaatt taagacgatc tatatggcta
     2161 aaaagccggt tcaactgcca ggttactact acgtggacac caaactggat atcacgtctc
     2221 acaacgaaga ttataccatc gtcgaacagt atgaacgtag cgagggccgt caccacctgt
     2281 tcctgggcca cggtactggt tctacgggtt ctggttcctc tggcactgcg tcttccgaag
     2341 acaacaacat ggcggtgatc aaagagttca tgaggttcaa agtacgtatg gaaggttcta
     2401 tgaacggcca tgagttcgag attgaaggtg agggcgaggg tcgcccatac gagggcactc
     2461 agaccgcaaa gctgaaagtt accaaaggtg gcccactgcc ttttgcgtgg gatatcctgt
     2521 ctccgcagtt catgtacggt tccaaagcct acgtaaaaca cccggcagac atcccggatt
     2581 acaaaaagct gtccttccca gagggcttca aatgggaacg tgttatgaat ttcgaagatg
     2641 gcggcctggt cactgttacg caggacagca gcctgcaaga cggtactctg atttacaaag
     2701 tgaaaatgcg tggcactaac ttcccgcctg acggtccagt aatgcagaag aaaactatgg
     2761 gctgggaagc tagcaccgaa cgtctgtacc cgcgcgacgg tgtgctgaaa ggcgagattc
     2821 accaggccct gaagctgaag gatggtggtc actatctggt tgagttcaaa actatctaca
     2881 tggcaaaaaa gcctgtacaa ctgccgggct attactacgt tgacactaaa ctggacatca
     2941 cctcccacaa cgaggattat actattgttg aacagtacga gcgttctgaa ggtcgccatc
     3001 atctgtttct gtatggtatg gacgaactgt ataaataaaa gcttatcgat aatcaacctc
     3061 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     3121 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     3181 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     3241 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     3301 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     3361 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     3421 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     3481 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     3541 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     3601 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgac
     3661 ccgggcggcc gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa
     3721 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     3781 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     3841 aggttcaggg ggagatgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
     3901 aaatcgataa ggatcttcct agagcatggc tacgtagata agtagcatgg cgggttaatc
     3961 attaactaca aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg
     4021 ctcactgagg ccgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct
     4081 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     4141 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     4201 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     4261 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     4321 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     4381 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     4441 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     4501 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     4561 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     4621 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     4681 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     4741 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     4801 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     4861 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     4921 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     4981 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     5041 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     5101 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     5161 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     5221 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     5281 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     5341 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     5401 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     5461 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     5521 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     5581 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     5641 attctgagaa tagtgtatgc ggcgaccgag ttgctc
//
