LOCUS       sequence-474430-        3552 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mycobacterium tuberculosis Encapsulin CFP29 expression plasmid in E.
            coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Berger C, Lewis C, Gao Y, Knoops K, Lopez-Iglesias C, Peters PJ, 
            Ravelli RBG
  TITLE     In situ and in vitro cryo-EM reveal structures of mycobacterial 
            encapsulin assembly intermediates.
  JOURNAL   Commun Biol. 2025 Feb 15;8(1):245. doi: 10.1038/s42003-025-07660-5.
  PUBMED    39955411
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2025-02-15"; volume: "8(1):245. doi"; pages: " 
            10.1038/s42003-025-07660-5"
COMMENT     Sequence Label: pRSET-cfp29
FEATURES             Location/Qualifiers
     source          1..3552
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(855..873)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      869..916
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1013..1468
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1100..1119)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1310..1331
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1494..1598
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1599..1667,1668..2459)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1817..1836)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2630..3218
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3119..3138
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3372..3389
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     3474..3493
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3474..3492
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             3524..3546
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tatgaacaac ctgtatcgcg atctggcgcc agttactgaa gccgcatggg cagagattga
       61 gctggaagcg gcacgcacct tcaagcgtca cattgcgggt cgtcgcgtag tggacgtgag
      121 cgatcctggt ggccccgtta cagcagccgt gtccactggt cggctgatcg acgtgaaagc
      181 gccaaccaat ggggttattg cccatctgcg tgcgagcaaa ccgcttgttc gcttacgtgt
      241 acccttcacc ttgtcacgca acgagatcga cgatgttgaa cgcgggtcta aagactcgga
      301 ttgggaaccg gtcaaagaag cggcaaagaa actggccttt gtcgaagatc gcacgatctt
      361 tgagggctat agtgccgcat cgattgaggg cattcggagt gctagctcta atccggctct
      421 gactctgccg gaagatccgc gtgagattcc ggatgtcatc agccaagcgc tgtcggaatt
      481 acgccttgcc ggcgtggatg gcccatactc cgtcctgttg agcgctgatg tctacaccaa
      541 agtgagtgaa acgagcgatc atggctatcc gattcgtgaa cacttgaatc gcctggtaga
      601 tggcgatatc atttgggctc ctgcgattga tggtgccttt gtgctgacca cacgtggagg
      661 agacttcgac ttacagctcg gtaccgacgt tgccatcggt tacgcgtcac acgacacgga
      721 taccgtgcgc ctctatctgc aggaaaccct cacgtttctg tgctatacgg ctgaagcgtc
      781 tgtggcgctt tcccattaaa agcttgatcc ggctgctaac aaagcccgaa aggaagctga
      841 gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt
      901 cttgaggggt tttttgctga aaggaggaac tatatccgga tctggcgtaa tagcgaagag
      961 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     1021 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     1081 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     1141 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     1201 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     1261 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     1321 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     1381 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     1441 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     1501 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     1561 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     1621 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     1681 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     1741 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     1801 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     1861 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     1921 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     1981 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     2041 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     2101 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     2161 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     2221 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     2281 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     2341 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     2401 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     2461 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     2521 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     2581 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     2641 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     2701 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     2761 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2821 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2881 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2941 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3001 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3061 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3121 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3181 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3241 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     3301 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     3361 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     3421 ctctccccgc gcgttggccg attcattaat gcaggatctc gatcccgcga aattaatacg
     3481 actcactata gggagaccac aacggtttcc ctctagaaat aattttgttt aactttaaga
     3541 aggagatata ca
//
