LOCUS       sequence-474458-       10513 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpression KLF5 ORF from a EF1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10513)
  AUTHORS   Southard KM, Ardy RC, Tang A, O'Sullivan DD, Metzner E, 
            Guruvayurappan K, Norman TM
  TITLE     Comprehensive transcription factor perturbations recapitulate 
            fibroblast transcriptional states.
  JOURNAL   Nat Genet. 2025 Aug 6. doi: 10.1038/s41588-025-02284-1.
  PUBMED    40770575
REFERENCE   2  (bases 1 to 10513)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2025 Aug 6. doi: 10.1038/s41588-025-02284-1."
COMMENT     Sequence Label: pRCA903 - KLF5 isoform 1 Puro-T2A-BFP (pRCA845...
FEATURES             Location/Qualifiers
     source          1..10513
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2616
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             2629..2685
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2686..3393
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDM
                     ALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVA
                     RYCDLPSKLGHKLN"
     misc_feature    3439..3556
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3694..4872
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3925..4863
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4820..4840
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4883..4892
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6370..6958
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6423..6443)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6841..6852)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7030..7263
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7341..7462
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7378..7397)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7432..7451
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7502..7637
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7657..7676)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7658..7676)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7686..7703)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7686..7702)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7695..7717)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7844..8299
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7931..7950)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8141..8162
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8325..8429
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8430..8498,8499..9290)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8648..8667)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9461..10049
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9950..9969
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10203..10220
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10337..10358
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10373..10378,10379..10396,10397..10403)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10411..10427
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10416..10438
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10435..10451
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10435..10451
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10470..10490
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10472..10490
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtg gatcgggagc tactaacttc
     2641 agcctgctga agcaggctgg agacgtggag gagaaccctg gacctgtgtc taagggcgaa
     2701 gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt ggacaaccat
     2761 cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga
     2821 atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc tactagcttc
     2881 ctctacggca gcaagacctt catcaaccac acccagggca tccccgactt cttcaagcag
     2941 tccttccctg agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg
     3001 accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga
     3061 ggggtgaact tcacatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc
     3121 ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag gcagaaacga catggccctg
     3181 aagctcgtgg gcgggagcca tctgatcgca aacgccaaga ccacatatag atccaagaaa
     3241 cccgctaaga acctcaagat gcctggcgtc tactatgtgg actacagact ggaaagaatc
     3301 aaggaggcca acaacgagac ctacgtcgag cagcacgagg tggcagtggc cagatactgc
     3361 gacctcccta gcaaactggg gcacaagctt aattgacgaa ttctagatct tgagacaaat
     3421 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     3481 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     3541 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt ggcgccggct
     3601 cgaggcctgc aggtgcaaag atggataaag ttttaaacag agaggaatct ttgcagctaa
     3661 tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag
     3721 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc
     3781 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     3841 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     3901 aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc
     3961 tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt
     4021 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga
     4081 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga
     4141 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat
     4201 ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa
     4261 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc
     4321 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg
     4381 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg
     4441 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt
     4501 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt
     4561 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc
     4621 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg
     4681 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag
     4741 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag
     4801 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt
     4861 caggtgtcgt gaggctagca ccgccaccat ggctacaagg gtgctgagca tgagcgcccg
     4921 cctgggaccc gtgccccagc cgccggcgcc gcaggacgag ccggtgttcg cgcagctcaa
     4981 gccggtgctg ggcgccgcga atccggcccg cgacgcggcg ctcttccccg gcgaggagct
     5041 gaagcacgcg caccaccgcc cgcaggcgca gcccgcgccc gcgcaggccc cgcagccggc
     5101 ccagccgccc gccaccggcc cgcggctgcc tccagaggac ctggtccaga caagatgtga
     5161 aatggagaag tatctgacac ctcagcttcc tccagttcct ataattccag agcataaaaa
     5221 gtatagacga gacagtgcct cagtcgtaga ccagttcttc actgacactg aagggttacc
     5281 ttacagtatc aacatgaacg tcttcctccc tgacatcact cacctgagaa ctggcctcta
     5341 caaatcccag agaccgtgcg taacacacat caagacagaa cctgttgcca ttttcagcca
     5401 ccagagtgaa acgactgccc ctcctccggc cccgacccag gccctccctg agttcaccag
     5461 tatattcagc tcacaccaga ccgcagctcc agaggtgaac aatattttca tcaaacaaga
     5521 acttcctaca ccagatcttc atctttctgt ccctacccag cagggccacc tgtaccagct
     5581 actgaataca ccggatctag atatgcccag ttctacaaat cagacagcag caatggacac
     5641 tcttaatgtt tctatgtcag ctgccatggc aggccttaac acacacacct ctgctgttcc
     5701 gcagactgca gtgaaacaat tccagggcat gcccccttgc acatacacaa tgccaagtca
     5761 gtttcttcca caacaggcca cttactttcc cccgtcacca ccaagctcag agcctggaag
     5821 tccagataga caagcagaga tgctccagaa tttaacccca cctccatcct atgctgctac
     5881 aattgcttct aaactggcaa ttcacaatcc aaatttaccc accaccctgc cagttaactc
     5941 acaaaacatc caacctgtca gatacaatag aaggagtaac cccgatttgg agaaacgacg
     6001 catccactac tgcgattacc ctggttgcac aaaagtttat accaagtctt ctcatttaaa
     6061 agctcacctg aggactcaca ctggtgaaaa gccatacaag tgtacctggg aaggctgcga
     6121 ctggaggttc gcgcgatcgg atgagctgac ccgccactac cggaagcaca caggcgccaa
     6181 gcccttccag tgcggggtgt gcaaccgcag cttctcgcgc tctgaccacc tggccctgca
     6241 tatgaagagg caccagaact agtaatgaac tagtccgtaa cttgaaagta tttcgatttc
     6301 ttggctttat atatcttgtg gaaaggacga gggttatccg ctcgtcggaa gttaacgcgt
     6361 taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     6421 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     6481 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     6541 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     6601 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     6661 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     6721 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     6781 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     6841 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     6901 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt
     6961 cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     7021 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     7081 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     7141 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     7201 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     7261 gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     7321 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     7381 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     7441 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     7501 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     7561 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     7621 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     7681 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     7741 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     7801 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     7861 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     7921 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     7981 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     8041 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     8101 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     8161 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     8221 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     8281 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     8341 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     8401 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     8461 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     8521 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     8581 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     8641 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     8701 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     8761 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     8821 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     8881 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     8941 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     9001 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     9061 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     9121 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     9181 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     9241 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     9301 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     9361 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     9421 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     9481 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     9541 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     9601 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     9661 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     9721 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     9781 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     9841 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     9901 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     9961 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
    10021 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
    10081 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
    10141 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
    10201 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
    10261 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
    10321 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
    10381 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
    10441 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
    10501 tggagctgca agc
//
