LOCUS       sequence-474464-        8915 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with
            cs1 incorporated in the loop of the sgRNA constant region for 10X 
            Direct Capture.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8915)
  AUTHORS   Southard KM, Ardy RC, Tang A, O'Sullivan DD, Metzner E, 
            Guruvayurappan K, Norman TM
  TITLE     Comprehensive transcription factor perturbations recapitulate 
            fibroblast transcriptional states.
  JOURNAL   Nat Genet. 2025 Aug 6. doi: 10.1038/s41588-025-02284-1.
  PUBMED    40770575
REFERENCE   2  (bases 1 to 8915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2025 Aug 6. doi: 10.1038/s41588-025-02284-1."
COMMENT     Sequence Label: pRCA595 - pBA904 Puro-T2A-mCherry
FEATURES             Location/Qualifiers
     source          1..8915
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(77..868,869..937))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     700..719
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(938..1042)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1117..1136)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1308..1687
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1689..1887
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1842..1862
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1905..2085
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2132..2257
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2754..2987
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3172..3216
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3514..3631
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(3884..3917)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     3921..3941
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        4212..4423
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          4424..5362
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5319..5339
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5383..5979
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5383..5402)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5879..5899
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5989..6042
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      6049..6058
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6055..6765
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6200..6218)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6468..6487
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(6785..6818)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6874..7462
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6927..6947)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7345..7356)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7465..7481)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7466..7482)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7991..8171
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8233..8821)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8313..8332)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
       61 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
      121 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
      181 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
      241 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
      301 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
      361 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
      421 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
      481 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
      541 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
      601 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
      661 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
      721 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
      781 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
      841 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
      901 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
      961 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     1021 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     1081 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
     1141 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
     1201 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
     1261 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt
     1321 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     1381 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     1441 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     1501 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     1561 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     1621 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     1681 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     1741 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     1801 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     1861 tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag
     1921 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     1981 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     2041 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     2101 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     2161 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     2221 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     2281 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     2341 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     2401 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     2461 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     2521 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     2581 caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     2641 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     2701 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     2761 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     2821 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     2881 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     2941 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     3001 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     3061 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     3121 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     3181 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     3241 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     3301 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     3361 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     3421 ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact gcgtgcgcca
     3481 attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     3541 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     3601 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     3661 agtttggtta gtaccgggcc cgctctagag atccgacgcg ccatctctag gcccgcgccg
     3721 gccccctcgc acggacttgt gggagaagct cggctactcc cctgccccgg ttaatttgca
     3781 tataatattt cctagtaact atagaggctt aatgtgcgat aaaagacaga taatctgttc
     3841 tttttaatac tagctacatt ttacatgata ggcttggatt tctataactt cgtatagcat
     3901 acattatacg aagttataaa cagcacaaaa ggaaactcac cctaactgta aagtaattgt
     3961 gtgttttgag actataagta tcccttggag aaccaccttg ttggaccagg atgggcacca
     4021 cccgtttaag agctaagctg gaaacagcat agcaagttta aataaggcta gtccgttatc
     4081 aacttggccg ctttaaggcc ggtcctagca aggccaagtg gcaccgagtc ggtgcttttt
     4141 ttctcgagta ctaggatcca ttaggcggcc gcgtggataa ccgtattacc gccatgcatg
     4201 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
     4261 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
     4321 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
     4381 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
     4441 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
     4501 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
     4561 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
     4621 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
     4681 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
     4741 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
     4801 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
     4861 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
     4921 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
     4981 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
     5041 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
     5101 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
     5161 gcgcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
     5221 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
     5281 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
     5341 aagttttttt cttccatttc aggtgtcgtg agctagccca ccatgaccga gtacaagccc
     5401 acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg
     5461 ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc
     5521 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     5581 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     5641 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     5701 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     5761 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     5821 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     5881 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     5941 ggaccgcgca cctggtgcat gacccgcaag cccggtgccg gatcgggaga gggcagagga
     6001 agtctgctaa catgcggtga cgtcgaggag aatcctggcc caccggtcgc caccatggtg
     6061 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
     6121 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     6181 tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc
     6241 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc
     6301 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     6361 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
     6421 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
     6481 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
     6541 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc
     6601 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc
     6661 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
     6721 gagggccgcc actccaccgg cggcatggac gagctgtaca agtaagaatt cgtcgaggga
     6781 cctaataact tcgtatagca tacattatac gaagttatac atgtttaagg gttccggttc
     6841 cactaggtac aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt
     6901 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     6961 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     7021 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     7081 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     7141 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     7201 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     7261 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     7321 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     7381 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     7441 tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gatcgagacc tagaaaaaca
     7501 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     7561 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
     7621 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     7681 aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     7741 cttccctgat tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg
     7801 atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga
     7861 gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
     7921 attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc
     7981 ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     8041 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     8101 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     8161 aatctctagc agcatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8221 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8281 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8341 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8401 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8461 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8521 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8581 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8641 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     8701 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8761 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8821 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8881 agggattttg gtcatgagat tatcaaaaag gatct
//
