LOCUS       sequence-474485-       14052 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses split luciferase fusion cLUC-NLRb in planta.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14052)
  AUTHORS   Chen R, Chen J, Outram MA, Powell OR, Arndell T, Gajendiran K, Wang 
            YL, Ayliffe MA, Blundell C, Figueroa M, Sperschneider J, Vanhercke 
            T, Tang D, Xu Y, Zhong G, Gardener C, Yu G, Gourdoupis S, Jaremko Ł,
            Matny O, Steffenson BJ, Boshoff WHP, Meyer WB, Arold ST, Dodds PN, 
            Wulff BBH
  TITLE     A wheat tandem kinase sensor activates an NLR helper to trigger 
            immunity
  JOURNAL   bioRxiv 2024.08.30.610287
REFERENCE   2  (bases 1 to 14052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.08.30.610287"
COMMENT     Sequence Label: pCambia1300_cLUC-NLRb
FEATURES             Location/Qualifiers
     source          1..14052
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        501..846
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      4506..5147
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     primer_bind     complement(5155..5172)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5155..5171)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5164..5186)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    5374..5398
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6699..7328
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7757..8830
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8896..9090
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9326..9348)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9434..9574
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9489..9508
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9589..9606)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9760..10348)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9840..9859)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10435..11229)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11654..11678
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    11756..11930
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(11971..12996)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDDLMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(13063..13739)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    13930..13951
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13966..13971,13972..13989,13990..13996)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14004..14020
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14009..14031
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14028..14044
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14028..14044
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gaattcggtc cccagattag ccttttcaat ttcagaaaga atgctaaccc acagatggtt
       61 agagaggctt acgcagcagg tctcatcaag acgatctacc cgagcaataa tctccaggaa
      121 atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taactgcatc
      181 aagaacacag agaaagatat atttctcaag atcagaagta ctattccagt atggacgatt
      241 caaggcttgc ttcacaaacc aaggcaagta atagagattg gagtctctaa aaaggtagtt
      301 cccactgaat caaaggccat ggagtcaaag attcaaatag aggacctaac agaactcgcc
      361 gtaaagactg gcgaacagtt catacagagt ctcttacgac tcaatgacaa gaagaaaatc
      421 ttcgtcaaca tggtggagca cgacacactt gtctactcca aaaatatcaa agatacagtc
      481 tcagaagacc aaagggcaat tgagactttt caacaaaggg taatatccgg aaacctcctc
      541 ggattccatt gcccagctat ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc
      601 tcctacaaat gccatcattg cgataaagga aaggccatcg ttgaagatgc ctctgccgac
      661 agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca
      721 accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca
      781 caatcccact atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag
      841 agaacacggg ggacgagctc atgtccggtt atgtaaacaa tccggaagcg accaacgcct
      901 tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac
      961 acttcttcat cgttgaccgc ctgaagtctc tgattaagta caaaggctat caggtggctc
     1021 ccgctgaatt ggaatccatc ttgctccaac accccaacat cttcgacgca ggtgtcgcag
     1081 gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa
     1141 agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa
     1201 agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg
     1261 acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag atcgccgtgg
     1321 gaggtggctc atctggcgga ggtcagatct cgtacgcgtc ccggggcggt accggcatgg
     1381 aggagatcgt gcttggactg tccaagacgg tggtggaggg gacgctggtc aaggtgaagg
     1441 ccgcgattga cgaggaggcg aagctgaagc ttgccgtgca gagcgacctg gtgttcatca
     1501 cgggggagtt cgagatgatg cagtcgttcc tcaacgtcgc cgacgcggag cgcatcaaga
     1561 acaacgccgt caagacgtgg gtgcggcagc tccgcgacct ggcctacgac accgaggact
     1621 gcatcgagct ggtggtccac ctggatccca agcccagatg gtggcgccgc ctgctggtcc
     1681 tgccttgcct gccggcggtg tcgctgccaa tggacaacgc ggcggcggag ataaaggagc
     1741 tcaaggaccg ggtggagttc gtgagccaga ggaacatgcg ctacaagctc atcaccgact
     1801 tcggcggcgc caagtccgtc gcgcagcagc agcttgaccg ggccgccgga gctctcgaca
     1861 tcgccgtgga ggaggcggcg aagaaaggtc gtagcttggt ggatctgacc gagttgatcc
     1921 ccagaatgga ggaccgccct gagctcggcg tgatctcggt gtgggggacc ggcggcgacc
     1981 ttggggtggc gtccatcgtc aggaaggcct acgacgaccc tgaaatttgc aagaacttcc
     2041 atagtcgtgg ctgggccaag ttgacccacc ctttcgatcc gcggaagatc ctccggagct
     2101 tgttcattca gttctgcact aacactgctg ctccacagaa gcaaggggaa actctggatg
     2161 tagattccct tctaaggatg gaaaagacgg tggtggaaga aggggagctg gtcaagcagt
     2221 ttgtgagtca tgtgagcacc cacaggttcc tcgttgtcct cgaaggcgtg tcaacgatgg
     2281 cggaatggga tgccctccgg atgtacctgc cagacatggg taacgggagc cagatcatcg
     2341 tatccacaca gcattttgac atcgcgagct tgtgcaccgg gcagctgcac aaagtttcag
     2401 agttgagaaa attcacacct gaccactctg tttgtgtctt tttcaaggaa gtttccggag
     2461 gtgatgatcc tggaaatgca atgttggagg gagatgacga aactgaactt attgggcgcg
     2521 acacagaaga ggacgagctt atggagcaga ttgatgaggt ttcccgtgtg atctccgtat
     2581 gggggattgc tggtgtagga aaatcggcac ttgtcagaag tgcctactgc aagtatctcc
     2641 aggaccgccc caacttcgcc gggttaaatc tgacctacat acacgggcgc gtgaacgtgc
     2701 cccatccctt caatctgagg gagttctgcc gcagcttgct cctgaacttg tcctctgaac
     2761 ctgctaaaac caaagaggac gccgtcgctg aactggcaaa catgaccaat ccaattgatg
     2821 tctgccgtaa gcttcttagc gacgatcaac agcagaagca gtgccttatt gttattgatg
     2881 acctccagtc cactgaagaa tgggacagaa taaaacaagc cttgtcattt ggtaaagaat
     2941 gttgtatcat agttgttaca aatgattcaa gtgttgccaa atattgttca gggcaagatc
     3001 agaaactcat gttcaacgtc aaaggtcttg gtgacgagca tgcccttgac ctcttcaaca
     3061 cggtatatga cggcgtgggt aaccacatca cacgggatga ggacatgatc aatcagtcga
     3121 agctaatgtt aaacaaatgc ggcggactac ccaaggtgat agttgcaatt ggttgtttct
     3181 tggccaagag tctgaattgg gaggccatga atactaactt tattcaccag ttggaaaaca
     3241 acccggagct ggctagtgta catagcatgt ttagttggct agactcctac ttccacaatt
     3301 gtcctgacga actcaagcca tgcatcttct atttgtcaat cttccctcga cgccacggca
     3361 ttcgtcgaag gcgtctggtg aggcggtgga ttgccgaggg ctactcaaga gacaccgacg
     3421 ggaatctcgc ggaagtgaac ggggaaaatt acttctccag gctcgtcaac ctcagcatgc
     3481 tcacagatgc tgagcgtgat gtcaacgtgg ctgggacagc gactggggct ggtactggga
     3541 ggagaatggc catgtgcaac gtcaacgatt tcttccggga gtacattgtg tcgcggcgaa
     3601 tggaggagaa ccatgtgttt gcactggagg ggaggtgcag ccagaccacg cggcgcactg
     3661 gacggcacct ggtcatcgac aaaagctggg acggggacga gagcgtgttc aacaggatcg
     3721 agttctcgcg actgcggtcg gtgacggtgt tcggaccctg gaagccattc ctggcctccg
     3781 acaagatgag ggtgctccgc gtgcttgatc tggaaggcac tgagggtctg acagacaacg
     3841 acatcaagaa catcgtcaaa cggctgcctc gactaaagtt cctctccctg cgaggatgca
     3901 agcacatctt tcggctgcca aaatccctgg gtcgcctgag gcagctccag accctggatg
     3961 tgaggcacac tgccgtagcc gggctgccag cgaccgtcgt gaagctacag aagctccagt
     4021 atgttcgtgc cggcaacaca ccagaggagt atgccgtcag cgacagcacg gaatctagca
     4081 cctcagtacc cacctgtttc tcatgtggcc ctgttggcaa tcacattgtc ggcgttgagg
     4141 tgcctcgcgg ggtcgagaat ctgaccaacc tgcacacact gggcgtcatc aaagccactg
     4201 tggcaggact caaggagctc aagaagctta cccagctgcg caagctcggc gtgtctggca
     4261 taaaccggag gaaccacaaa gagctctgcg ccgtcatctc gggccacggc catctggaat
     4321 ctctgtcgat atggtttgac aaggacaccg agaggattgc tgacccttgt tcgccgagca
     4381 atgatgactt caagcctcca gagaagctac ggaggctcaa actccacggg cacactgaca
     4441 acctgccagc atgggttagc actctcaaaa tatttgtcac tcaagatcaa tgagtcgacc
     4501 tgcagagctt tcgttcgtat catcggtttc gacaacgttc gtcaagttca atgcatcagt
     4561 ttcattgcgc acacaccaga atcctactga gtttgagtat tatggcattg ggaaaactgt
     4621 ttttcttgta ccatttgttg tgcttgtaat ttactgtgtt ttttattcgg ttttcgctat
     4681 cgaactgtga aatggaaatg gatggagaag agttaatgaa tgatatggtc cttttgttca
     4741 ttctcaaatt aatattattt gttttttctc ttatttgttg tgtgttgaat ttgaaattat
     4801 aagagatatg caaacatttt gttttgagta aaaatgtgtc aaatcgtggc ctctaatgac
     4861 cgaagttaat atgaggagta aaacacttgt agttgtacca ttatgcttat tcactaggca
     4921 acaaatatat tttcagacct agaaaagctg caaatgttac tgaatacaag tatgtcctct
     4981 tgtgttttag acatttatga actttccttt atgtaatttt ccagaatcct tgtcagattc
     5041 taatcattgc tttataatta tagttatact catggatttg tagttgagta tgaaaatatt
     5101 ttttaatgca ttttatgact tgccaattga ttgacaacat gcatcaagct tggcactggc
     5161 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     5221 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     5281 ccaacagttg cgcagcctga atggcgaatg ctagagcagc ttgagcttgg atcagattgt
     5341 cgtttcccgc cttcagttta aactatcagt gtttgacagg atatattggc gggtaaacct
     5401 aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa aaggtttatc
     5461 cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc aaagtacttt
     5521 gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc agccgtcttc
     5581 tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc tgcccttttc
     5641 ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg actagaaccg
     5701 gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg cccgcgtcag
     5761 caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa
     5821 gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct
     5881 tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc tggcccgcag
     5941 cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg gcctgcgtag
     6001 cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt tgaccgtgtt
     6061 cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga gcgggcgcga
     6121 ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc cggcacagat
     6181 cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg cggctgcact
     6241 gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc
     6301 caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg ccgacgccct
     6361 ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca ggacggccag
     6421 gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg
     6481 ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct
     6541 gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga gcgccgccgt
     6601 ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg
     6661 atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc gctgtactta
     6721 accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc gccctgcaac
     6781 tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg
     6841 cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc
     6901 gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg
     6961 cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg gtgcagccaa
     7021 gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag cgcattgagg
     7081 tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca
     7141 tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt gagtcccgta
     7201 tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag
     7261 aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac
     7321 tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc taagtgccgg
     7381 ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag acacgccagc
     7441 catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga agatgtacgc
     7501 ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc agctaccaga
     7561 gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg
     7621 aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg
     7681 gttggccagg cgtaagcggc tgggttgcct gccggccctg caatggcact ggaaccccca
     7741 agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc
     7801 gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat
     7861 cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga
     7921 atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga
     7981 gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat
     8041 catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg
     8101 ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggccagtgt
     8161 gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata
     8221 ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact
     8281 caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat
     8341 tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct
     8401 ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac
     8461 cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg agatcacaga
     8521 aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat
     8581 cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt
     8641 gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac
     8701 cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg
     8761 gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc
     8821 cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg
     8881 aaaaggtctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa
     8941 ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt
     9001 gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa
     9061 aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagagct
     9121 gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc
     9181 tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg
     9241 cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca ccctgcctcg
     9301 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     9361 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     9421 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct
     9481 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     9541 gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga
     9601 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     9661 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     9721 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     9781 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     9841 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     9901 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     9961 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
    10021 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
    10081 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
    10141 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
    10201 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
    10261 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
    10321 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
    10381 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcattcta ggtactaaaa
    10441 caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga tccccagtaa
    10501 gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg accggacgca
    10561 gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa taaagccact
    10621 tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg aaaagacaag
    10681 ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg
    10741 agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca gtaagtaatc
    10801 caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata tgtcgatgga
    10861 gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc tttgttcatc
    10921 ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat tgctccagcc
    10981 atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca gccatagcat
    11041 catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt ccgtcatttt
    11101 taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag acattccttc
    11161 cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg atattctcat
    11221 tttagccatt tattatttcc ttcctctttt ctacagtatt taaagatacc ccaagaagct
    11281 aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct taaataccag
    11341 aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc gacggagccg
    11401 attttgaaac cgcggtgatc acaggcagca acgctctgtc atcgttacaa tcaacatgct
    11461 accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt cttccgaata
    11521 gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg acgccgtccc
    11581 ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct
    11641 gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta
    11701 ataacacatt gcggacgttt ttaatgtact gaattaacgc cgaattaatt cgggggatct
    11761 ggattttagt actggatttt ggttttagga attagaaatt ttattgatag aagtatttta
    11821 caaatacaaa tacatactaa gggtttctta tatgctcaac acatgagcga aaccctatag
    11881 gaaccctaat tcccttatct gggaactact cacacattat tatggagaaa ctcgagcttg
    11941 tcgatcgaca gatcccggtc ggcatctact ctatttcttt gccctcggac gagtgctggg
    12001 gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct
    12061 tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca
    12121 tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg
    12181 gtcaagacca atgcggagca tatacgcccg gagtcgtggc gatcctgcaa gctccggatg
    12241 cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa
    12301 gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc
    12361 tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg
    12421 ccggacttcg gggcagtcct cggcccaaag catcagatca tcgagagcct gcgcgacgga
    12481 cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc
    12541 gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa
    12601 cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atagcctccg cgaccggttg
    12661 tagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct gtgaacggcg
    12721 ggagatgcaa taggtcaggc tctcgctaaa ctccccaatg tcaagcactt ccggaatcgg
    12781 gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac catcggcgca
    12841 gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag cacgagattc
    12901 ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga tcagaaactt
    12961 ctcgacagac gtcgcggtga gttcaggctt tttcatatct cattgccccc cggatctgcg
    13021 aaagctcgag agagatagat ttgtagagag agactggtga tttcagcgtg tcctctccaa
    13081 atgaaatgaa cttccttata tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc
    13141 atcccttacg tcagtggaga tatcacatca atccacttgc tttgaagacg tggttggaac
    13201 gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc
    13261 agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc
    13321 ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag
    13381 gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt
    13441 atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttca catcaatcca
    13501 cttgctttga agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg
    13561 gtccatcttt gggaccactg tcggcagagg catcttgaac gatagccttt cctttatcgc
    13621 aatgatggca tttgtaggtg ccaccttcct tttctactgt ccttttgatg aagtgacaga
    13681 tagctgggca atggaatccg aggaggtttc ccgatattac cctttgttga aaagtctcaa
    13741 tagccctttg gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt
    13801 gctccaccat gttggcaagc tgctctagcc aatacgcaaa ccgcctctcc ccgcgcgttg
    13861 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
    13921 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
    13981 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
    14041 tgacatgatt ac
//
