LOCUS       sequence-474540-        6374 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6374)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_5070:Dendra
FEATURES             Location/Qualifiers
     source          1..6374
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2318..2341
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2598..3398
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3523..4111
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4012..4031
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgaccaacca ggaacagtcc ggggacggcg gccgtctggc
      121 cccacgcccc gtcgagcggc cacccgtcga cccggctgcg cagcgcacgt tcggcaggcc
      181 tgccggtgtc gaaggatcgt tcctcggcgc gggccagtac caggaccagg gcgagtacgc
      241 ccccaaggac cagccgcccg atcccgtgct ggccgaggcg ttcggacgcc cccgcggcac
      301 gtccgagacc ctgcaacgtc accccaccga cgccggagcg ctggaggccg agcgcctggg
      361 cgacgaggac gtcaccgacg atccgtggcg tgatccgtcc gcgcccgtcg cgctgggcac
      421 acccgcgcaa cccccgccac cgccggtcta tgtcaacgcg ccgaccggca agctcggact
      481 gcgggaggtg ctgttcggcg gccgcgtctc ctggatcgcg ctgctgacgc tggccctggt
      541 cgcggtgctg atcgcgttcg tcggcggctg ggtggggcag aagaccgcgg gcaccgtgca
      601 ggcgttcacc acctcgaagg tgacgctgga gaccggtgac catccggacc ccgacgcggg
      661 ccgcttcgag caggtcgccg ccgcggtggc cgattccgtc gtcacgatcg aggccaagag
      721 caagaccgag gggtcacagg gctcgggcgt cgtcgtcgac ggcaagggct acatcgtcac
      781 caacaaccac gtgatctccg aggccgcgac caagccggcc gacttccaga tcacggtcgt
      841 gttcaacgac ggcaaggaag ttcccgccaa cctcgtcggg cgtgatccca agaccgacct
      901 cgcggtgctc aaggtcgaca acgtcgacaa ccttacggtc gccaagctcg gcgactccga
      961 gaagatccgc gtcggcgagg aagtgatcgc cgcgggtgca ccgctcggcc tgcgcagcac
     1021 cgtgacccac ggcatcgtca gcgcgctgca ccggccggtt ccgctctcgg gtgacggctc
     1081 ggacaccgac accgtcatcg acggtgtgca gaccgacgcc tcgatcaatc acggcaactc
     1141 cggtggtccg ctgatcaaca tgaacgccga ggtcatcggc atcaacacgg cgggcaaatc
     1201 gctttctgac agcgcaagcg gcctcggctt cgcgatcccg gtcaacgagg tcaaggaggt
     1261 cgtggagacg ctggtcaagg acggcaaggt cgcgcatccg acgctgctgg tgaacgccgt
     1321 gacggtcagc aacagcgtgg cctccggcgc ccagatcaag aacgtggtcg agaacggccc
     1381 ggccggcaag gcgggcatgt tggagaacga cgtcgtggtg aaggtcggtg accgcaaggt
     1441 ggccgacgcc gacgagttcg tcgtcgcggt gcgtcagctc aagatcggcc aggaagcgcc
     1501 catcgaggtg gtgcgggacg gacgtccgct caccctgatg gtgacgccca tcggcgacga
     1561 ccaaaaagcc cagaagcttg gtgcggccgc gggcaacacg cctggcatca atctgatcaa
     1621 ggaggacatg cgtgtcaagg tgcatatgga gggcaacgtg aacggtcacg ccttcgtgat
     1681 cgagggggag ggtaagggta agccttacga agggacccag accgccaacc tcaccgtcaa
     1741 ggagggcgcc cctctcccat tctcgtacga tatcctcact accgcggtgc attacgggaa
     1801 ccgcgtgttc accaagtacc ccgaggatat ccctgactac ttcaagcaga gcttcccgga
     1861 gggatactcc tgggagcgga cgatgacctt cgaagacaag ggtatctgta cgatccgcag
     1921 cgacatctcg ctggaagggg actgtttctt ccagaacgtc cgtttcaagg ggaccaactt
     1981 tccgccgaac ggcccggtca tgcagaagaa gaccctcaag tgggagccga gcacggagaa
     2041 gctgcacgtt cgggacggcc tcctggtcgg caacatcaac atggcgctgc tgctggaagg
     2101 cggcggccac tacctatgcg acttcaagac cacgtacaaa gcgaagaagg tcgtccagct
     2161 gcccgacgcg cacttcgtgg accaccgcat cgaaatcctg gggaacgatt cggactacaa
     2221 caaggtcaag ttgtacgagc acgctgtggc acgctatagc ccgctcccgt cgcaggtctg
     2281 gttcgaaggc ggcggcgggg agaacctgta tttccaggac tacaaggacg acgacgacaa
     2341 gtgatttaaa taaccccttg gggcctctaa acgggtcttg aggggttttt tgttaactag
     2401 cgtacgatcg aattcctgca gctcacggta actgatgccg tatttgcagt accagcgtac
     2461 ggcccacaga atgatgtcac gctgaaaatg ccggcctttg aatgggttca tgtgcagctc
     2521 catcagcaaa aggggatgat aagtttatca ccaccgacta tttgcaacag tgccgttgat
     2581 cgtgctatga tcgactgatg tcatcagcgg tggagtgcaa tgtcgtgcaa tacgaatggc
     2641 gaaaagccga gctcatcggt cagcttctca accttggggt tacccccggc ggtgtgctgc
     2701 tggtccacag ctccttccgt agcgtccggc ccctcgaaga tgggccactt ggactgatcg
     2761 aggccctgcg tgctacgctg ggtccgggag ggacgctcgt catgccctcg tggtcaggtc
     2821 tggacgacga gccgttcgat cctgccacgt cgcccgttac accggacctt ggagttgtct
     2881 ctgacacatt ctggcgcctg ccaaatgtaa agcgcagcgc ccatccattt gcctttgcgg
     2941 cagcggggcc acaggcagag cagatcatct ctgatccatt gcccctgcca ccttactcgc
     3001 ctgcaagccc ggtcgcccgt gtccatgaac tcgatgggca ggtacttctc ctcggcgtgg
     3061 gacacgatgc caacacgacg ctgcatcttg ccgagttgat ggcaaaggtt ccctatgggg
     3121 tgccgagaca ctgcaccatt cttcaggatg gcaagttggt acgcgtcgat tatctcgaga
     3181 atgaccactg ctgtgagcgc tttgccttgg cggacaggtg gctcaaggag aagagccttc
     3241 agaaggaagg tccagtcggt catgcctttg ctcggttgat ccgctcccgc gacattgtgg
     3301 cgacagccct gggtcaactg ggccgagatc cgttgatctt cctgcatccg ccagaggcgg
     3361 gatgcgaaga atgcgatgcc gctcgccagt cgattggctg agctcatgag cggagaacga
     3421 gatgacgttg gaggggcaag gtcgcgctga ttgctggggc aacacggggg atccactagt
     3481 tccatgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3541 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3601 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3661 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     3721 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     3781 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     3841 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     3901 cctacagcgt gagctatgag aaagcgccac gcttcccgag gggagaaagg cggacaggta
     3961 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     4021 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     4081 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     4141 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     4201 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     4261 gcgcaacgcg tgcggccgcg gtacccgggg atcctctaga gtcgaccacc aagggcacca
     4321 tctctgcttg ggccaccccg ttggccgcag ccagctcgct gagagccgtg aacgacaggg
     4381 cgaacgccag cccgccgacg gcgagggttc cgaccgctgc aactcccggt gcaaccttgt
     4441 cccggtctat tctcttcact gcaccagctc caatctggtg tgaatgcccc tcgtctgttc
     4501 gcgcaggcgg ggggctctat tcgtttgtca gcatcgaaag tagccagatc agggatgcgt
     4561 tgcaaccgcg tatgcccagg tcagaagagt cgcacaagag ttgcagaccc ctggaaagaa
     4621 aaatggccag agggcgaaaa caccctctga ccagcggagc gggcgacggg aatcgaaccc
     4681 gcgtagctag tttggaagaa tgggtgtctg ccgaccacat atgggccggt caagataggt
     4741 ttttaccccc tctcggctgc atcctctaag tggaaagaaa ttgcaggtcg tagaagcgcg
     4801 ttgaagcctg agagttgcac aggagttgca acccggtagc cttgttcacg acgagaggag
     4861 acctagttgg cacgtcgcgg atggggatcg ctgaagactc agcgcagcgg gaggatccaa
     4921 gcctcatacg tcaacccgca ggacggtgtg aggtactacg cgctgcagac ctacgacaac
     4981 aagatggacg ccgaagcctg gctcgcgggc gagaagcggc tcatcgagat ggagacctgg
     5041 acccctccac aggaccgggc gaagaaggca gccgccagcg ccatcacgct ggaggagtac
     5101 acccggaagt ggctcgtgga gcgcgacctc gcagacggca ccagggatct gtacagcggg
     5161 cacgcggagc gccgcatcta cccggtgcta ggtgaagtgg cggtcacaga gatgacgcca
     5221 gctctggtgc gtgcgtggtg ggccgggatg ggtaggaagc acccgactgc ccgccggcat
     5281 gcctacaacg tcctccgggc ggtgatgaac acagcggtcg aggacaagct gatcgcagag
     5341 aacccgtgcc ggatcgagca gaaggcagcc gatgagcgcg acgtagaggc gctgacgcct
     5401 gaggagctgg acatcgtcgc cgctgagatc ttcgagcact accggatcgc ggcatacatc
     5461 ctggcgtgga cgagcctccg gttcggagag ctgatcgagc ttcgccgcaa ggacatcgtg
     5521 gacgacggca tgacgatgaa gctccgggtg cgccgtggcg cttcccgcgt ggggaacaag
     5581 atcgtcgttg gcaacgccaa gaccgtccgg tcgaagcgtc ctgtgacggt tccgcctcac
     5641 gtcgcggaga tgatccgagc gcacatgaag gaccgtacga agatgaacaa gggccccgag
     5701 gcattcctgg tgaccacgac gcagggcaac cggctgtcga agtccgcgtt caccaagtcg
     5761 ctgaagcgtg gctacgccaa gatcggtcgg ccggaactcc gcatccacga cctccgcgct
     5821 gtcggcgcta cgttcgccgc tcaggcaggt gcgacgacca aggagctgat ggcccgtctc
     5881 ggtcacacga ctcctaggat ggcgatgaag taccagatgg cgtctgaggc ccgcgacgag
     5941 gctatcgctg aggcgatgtc caagctggcc aagacctcct gaaacgcaaa aagcccccct
     6001 cccaaggaca ctgagtccta aagagggggg tttcttgtca gtacgcgaag aaccacgcct
     6061 ggccgcgagc gccagcaccg ccgctctgtg cggagacctg ggcaccagcc ccgccgccgc
     6121 caggagcatt gccgttcccg ccagaaatct agacggtgac cacaacgcgc ccgctttgat
     6181 cggggacgtc gaaatattgg atcgtcggca ccgtcacggc cgtgggaggc ggcacgatcc
     6241 gcgacgtgat gatcggccgc atccccacgg tgctgcgcag tgagctctac gccatcccgg
     6301 cgttgatctg tgcgttcgca cgcacaggcc cggtgtgaga agggtctctg acgagcggga
     6361 gaactcccta tcag
//
