LOCUS       sequence-474558-        8510 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8510)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 8510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_MERS-CoV-nsp3
FEATURES             Location/Qualifiers
     source          1..8510
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     435..454
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     688..705
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(864..891)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(983..1069)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1118..1140
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1132..1149
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1133..1149
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1165..1264
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(6985..7084)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(7101..7120)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7102..7120)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7125..7141)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7125..7141)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             7572..8363
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(8456..8510,1..534)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
       61 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
      121 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
      181 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
      241 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
      301 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
      361 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      421 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      481 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
      541 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
      601 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
      661 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
      721 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
      781 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
      841 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
      901 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
      961 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     1021 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     1081 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     1141 acggccagtc ttaagctcgg gccccaaata atgattttat tttgactgat agtgacctgt
     1201 tcgttgcaac aaattgatga gcaatgcttt tttataatgc caactttgta caaaaaagca
     1261 gaagggccgt caaggccagg atccatggga tccgcacctg taaaaaaagt agcctttggc
     1321 ggtgatcaag tacatgaggt tgctgctgta agaagtgtta ctgtcgagta caacattcat
     1381 gctgtattag acacactact tgcttcttct agtcttagaa cctttgttgt agataagtct
     1441 ttgtcaattg aggagtttgc tgacgtagta aaggaacaag tctcagactt gcttgttaaa
     1501 ttactgcgtg gaatgccgat tccagatttt gatttagacg attttattga cgcaccatgc
     1561 tattgcttta acgctgaggg tgatgcatcc tggtcttcta ctatgatctt ctctcttcac
     1621 cccgtcgagt gtgacgagga gtgttctgaa gtagaggctt cagatttaga agaaggtgaa
     1681 tcagagtgca tttctgagac ttcaactgaa caagttgacg tttctcatga gacttctgac
     1741 gacgagtggg ctgctgcagt tgatgaagcg ttccctctcg atgaagcaga agatgttact
     1801 gaatctgtgc aagaagaagc acaaccagta gaagtacctg ttgaagatat tgcgcaggtt
     1861 gtcatagctg acaccttaca ggaaactcct gttgtgcctg atactgttga agtcccaccg
     1921 caagtggtga aacttccgtc tgcacctcag actatccagc ccgaggtaaa agaagttgca
     1981 cctgtctatg aggctgatac cgaacagaca cagaatgtta ctgttaaacc taagaggtta
     2041 cgcaaaaagc gtaatgttga ccctttgtcc aattttgaac ataaggttat tacagagtgc
     2101 gttaccatag ttttaggtga cgcaattcaa gtagccaagt gctatgggga gtctgtgtta
     2161 gttaatgctg ctaacacaca tcttaagcat ggcggtggta tcgctggtgc tattaatgcg
     2221 gcttcaaaag gggctgtcca aaaagagtca gatgagtata ttctggctaa agggccgtta
     2281 caagtaggag attcagttct cttgcaaggc cattctctag ctaagaatat cctgcatgtc
     2341 gtaggcccag atgcccgcgc taaacaggat gtttctctcc ttagtaagtg ctataaggct
     2401 atgaatgcat atcctcttgt agtcactcct cttgtttcag caggcatatt tggtgtaaaa
     2461 ccagctgtgt cttttgatta tcttattagg gaggctaaga ctagagtttt agtcgtcgtt
     2521 aattcccaag atgtctataa gagtcttacc atagttgaca ttccacagag tttgactttt
     2581 tcatatgatg ggttacgtgg cgcaatacgt aaagctaaag attatggttt tactgttttt
     2641 gtgtgcacag acaactctgc taacactaaa gttcttagga acaagggtgt tgattatact
     2701 aagaagtttc ttacagttga cggtgtgcaa tattattgct acacgtctaa ggacacttta
     2761 gatgatatct tacaacaggc taataagtct gttggtatta tatctatgcc tttgggatat
     2821 gtgtctcatg gtttagactt aatgcaagca gggagtgtcg tgcgtagagt taacgtgccc
     2881 tacgtgtgtc tcctagctaa taaagagcaa gaagctattt tgatgtctga agacgttaag
     2941 ttaaaccctt cagaagattt tataaagcac gtccgcacta atggtggtta caattcttgg
     3001 catttagtcg agggtgaact attggtgcaa gacttacgct taaataagct cctgcattgg
     3061 tctgatcaaa ccatatgcta caaggatagt gtgttttatg ttgtaaagaa tagtacagct
     3121 tttccatttg aaacactttc agcatgtcgt gcgtatttgg attcacgcac gacacagcag
     3181 ttaacaatcg aagtcttagt gactgtcgat ggtgtaaatt ttagaacagt cgttctaaat
     3241 aataagaaca cttatagatc acagcttgga tgcgttttct ttaatggtgc tgatatttct
     3301 gacaccattc ctgatgagaa acagaatggt cacagtttat atctagcaga caatttgact
     3361 gctgatgaaa caaaggcgct taaagagtta tatggccccg ttgatcctac tttcttacac
     3421 agattctatt cacttaaggc tgcagtccat gggtggaaga tggttgtgtg tgataaggta
     3481 cgttctctca aattgagtga taataattgt tatcttaatg cagttattat gacacttgat
     3541 ttattgaagg acattaaatt tgttatacct gctctacagc atgcatttat gaaacataag
     3601 ggcggtgatt caactgactt catagccctc attatggctt atggcaattg cacatttggt
     3661 gctccagatg atgcctctcg gttacttcat accgtgcttg caaaggctga gttatgctgt
     3721 tctgcacgca tggtttggag agagtggtgc aatgtctgtg gcataaaaga tgttgttcta
     3781 caaggcttaa aagcttgttg ttacgtgggt gtgcaaactg ttgaagatct gcgtgctcgc
     3841 atgacatatg tatgccagtg tggtggtgaa cgtcatcggc aattagtcga acacaccacc
     3901 ccctggttgc tgctctcagg cacaccaaat gaaaaattgg tgacaacctc cacggcgcct
     3961 gattttgtag catttaatgt ctttcagggc attgaaacgg ctgttggcca ttatgttcat
     4021 gctcgcctga agggtggtct tattttaaag tttgactctg gcaccgttag caagacttca
     4081 gactggaagt gcaaggtgac agatgtactt ttccccggcc aaaaatacag tagcgattgt
     4141 aatgtcgtac ggtattcttt ggacggtaat ttcagaacag aggttgatcc cgacctatct
     4201 gctttctatg ttaaggatgg taaatacttt acaagtgaac cacccgtaac atattcacca
     4261 gctacaattt tagctggtag tgtctacact aatagctgcc ttgtatcgtc tgatggacaa
     4321 cctggcggtg atgctattag tttgagtttt aataaccttt tagggtttga ttctagtaaa
     4381 ccagtcacta agaaatacac ttactccttc ttgcctaaag aagacggcga tgtgttgttg
     4441 gctgagtttg acacttatga ccctatttat aagaatggtg ccatgtataa aggcaaacca
     4501 attctttggg tcaataaagc atcttatgat actaatctta ataagttcaa tagagctagt
     4561 ttgcgtcaaa tttttgacgt agcccccatt gaactcgaaa ataaattcac acctttgagt
     4621 gtggagtcta caccagttga acctccaact gtagatgtgg tagcacttca acaggaaatg
     4681 acaattgtca aatgtaaggg tttaaataaa cctttcgtga aggacaatgt cagtttcgtt
     4741 gctgatgatt caggtactcc cgttgttgag tatctgtcta aagaagacct acatacattg
     4801 tatgtagacc ctaagtatca agtcattgtc ttaaaagaca atgtactttc ttctatgctt
     4861 agattgcaca ccgttgagtc aggtgatatt aacgttgttg cagcttccgg atctttgaca
     4921 cgtaaagtga agttactatt tagggcttca ttttatttca aagaatttgc tacccgcact
     4981 ttcactgcta ccactgctgt aggtagttgt ataaagagtg tagtgcggca tctaggtgtt
     5041 actaaaggca tattgacagg ctgttttagt tttgccaaga tgttatttat gcttccacta
     5101 gcttacttta gtgattcaaa actcggcacc acagaggtta aagtgagtgc tttgaaaaca
     5161 gccggcgttg tgacaggtaa tgttgtaaaa cagtgttgca ctgctgctgt tgatttaagt
     5221 atggataagt tgcgccgtgt ggattggaaa tcaaccctac ggttgttact tatgttatgc
     5281 acaactatgg tattgttgtc ttctgtgtat cacttgtatg tcttcaatca ggtcttatca
     5341 agtgatgtta tgtttgaaga tgcccaaggt ttgaaaaagt tctacaaaga agttagagct
     5401 tacctaggaa tctcttctgc ttgtgacggt cttgcttcag cttatagggc gaattccttt
     5461 gatgtaccta cattctgcgc aaaccgttct gcaatgtgta attggtgctt gattagccaa
     5521 gattccataa ctcactaccc agctcttaag atggttcaaa cacatcttag ccactatgtt
     5581 cttaacatag attggttgtg gtttgcattt gagactggtt tggcatacat gctctatacc
     5641 tcggccttca actggttgtt gttggcaggt acattgcatt atttctttgc acagacttcc
     5701 atatttgtag actggcggtc atacaattat gctgtgtcta gtgccttctg gttattcacc
     5761 cacattccaa tggcgggttt ggtacgaatg tataatttgt tagcatgcct ttggctttta
     5821 cgcaagtttt atcagcatgt aatcaatggt tgcaaagata cggcatgctt gctctgctat
     5881 aagaggaacc gacttactag agttgaagct tctaccgttg tctgtggtgg aaaacgtacg
     5941 ttttatatca cagcaaatgg cggtatttca ttctgtcgta ggcataattg gaattgtgtg
     6001 gattgtgaca ctgcaggtgt ggggaatacc ttcatctgtg aagaagtcgc aaatgacctc
     6061 actaccgccc tacgcaggcc tattaacgct acggatagat cacattatta tgtggattcc
     6121 gttacagtta aagagactgt tgttcagttt aattatcgta gagacggtca accattctac
     6181 gagcggtttc ccctctgcgc ttttacaaat ctagataagt tgaagttcaa agaggtctgt
     6241 aaaactacta ctggtatacc tgaatacaac tttatcatct acgactcatc agatcgtggc
     6301 caggaaagtt tagctaggtc tgcatgtgtt tattattctc aagtcttgtg taaatcaatt
     6361 cttttggttg actcaagttt ggttacttct gttggtgatt ctagtgaaat cgccactaaa
     6421 atgtttgatt cctttgttaa tagtttcgtc tcgctgtata atgtcacacg cgataagttg
     6481 gaaaaactta tctctactgc tcgtgatggc gtaaggcgag gcgataactt ccatagtgtc
     6541 ttaacaacat tcattgacgc agcacgaggc cccgcaggtg tggagtctga tgttgagacc
     6601 aatgaaattg ttgactctgt gcagtatgct cataaacatg acatacaaat tactaatgag
     6661 agctacaata attatgtacc ctcatatgtt aaacctgata gtgtgtctac cagcgattta
     6721 ggtagtctca ttgattgtaa tgcggcttca gttaaccaaa ttgtcttgcg taattctaat
     6781 ggtgcttgca tttggaacgc tgctgcatat atgaaactct cggatgcact taaacgacag
     6841 attcgcattg catgccgtaa gtgtaattta gctttccggt taaccacctc aaagctacgc
     6901 gctaatgata atatcttatc agttagattc actgctaaca aaattgttgg tggtcttaat
     6961 taagacgcga tcgcggcctc atgggcccag ctttcttgta caaagttggc attataaaaa
     7021 ataattgctc atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
     7081 tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct
     7141 ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
     7201 catcatgcct cctctagacc agccaggaca gaaatgcctc gacttcgctg ctacccaagg
     7261 ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct
     7321 gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct
     7381 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
     7441 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
     7501 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
     7561 agttaaacat tatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
     7621 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     7681 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa
     7741 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     7801 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     7861 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     7921 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     7981 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     8041 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     8101 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     8161 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     8221 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
     8281 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga
     8341 tcaccaaggt agtcggcaaa taaccctcga gccacccatg accaaaatcc cttaacgtga
     8401 gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     8461 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
//
