LOCUS       sequence-474564-        9533 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpress Sc Pol3(C-terminal 3X FLAG tag) when integrated into 
            yeast (S. cer).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9533)
  TITLE     O'Donnell lab plasmids 2025
REFERENCE   2  (bases 1 to 9533)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Sc Pol3-FLAG(Ec)-pRSF405/GAL
FEATURES             Location/Qualifiers
     source          1..9533
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2359..3023
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     primer_bind     complement(2372..2395)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    2690..2807
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(3051..3067)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3052..3068)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(3092..3111)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3093..3111)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3121..3138)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3121..3137)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3130..3152)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3278..3733
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3365..3384)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3575..3596
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4034..4441
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             4442..5536
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(6030..6049)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6149..6171
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6209..6227)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6295..6399
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6400..6468,6469..7260)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6618..6637)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7431..8019
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7920..7939
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8173..8190
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8307..8328
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8343..8348,8349..8366,8367..8373)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8381..8397
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8386..8408
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8405..8421
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8405..8421
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8440..8460
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8442..8460
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 atccatagct tctttcaggt cggtggtacc gaatttaacc ataacggagt cagtatcgcc
       61 gtacactacg acagcgtcat gtttgtagcc gtttttgatg cagtattttt cttgaactgc
      121 ggttttggtt ttcaggatca tcgtgcggcc gtaagcagtg acgctggagg aaatggccag
      181 acatggcagt ttaccaacag tagcgccggt gaaaccgtaa acgctgttgg cggagatttt
      241 cagtgccagt tgacgaccgt tcagtacgtc acgtttgaac gggtcttttt cgtcgcgcag
      301 atctttcttc gcacgtttgc gcgcggaaat cagttcgtcc aggatgatcg gcagaatacc
      361 acgacgacgt ttggtcgtga cgaagtaatc gccatttggg gtgatcacgt aatcctcgtc
      421 gattttcagg ttcaggcgtt ctacggttgc tttgttgcac agagtagtgt aacacaggtt
      481 atgcgccatc ataatggacg ggtacaggct gttgaaatcc agagtagcga tcggtacatc
      541 gtagtagcca cggatcggct cgatcacggt ggcgccttca tactggtcat cggaggcctg
      601 gctctgcatg ttcgggatga cggtgtcgat ctccagacat ttgcggaaca gttgagacac
      661 cactttgatc tgttgaccgc gtgccagcag gtagctgaac ggaacgccgg ttacacgggc
      721 catttcggtg tagttgacca gcgccatcag tttctccatc aggcgcagcg gcaggtaggc
      781 gtctttcaga cagtacactg ccagacgacg gcgggtttca gagtcgccgt tctgcagatc
      841 agagatgata gagtagtgca cgtcctcttt ttgttcaccc aggaagtgcg cagaaactgc
      901 attcagagtg taggagcgca gtttgtactc gcgttggatg aactgcagca gatccagttg
      961 caggcgaccg tcaatgttaa cgtttttcgt ttcgcgagtg ccgtaagctt tggaagaaaa
     1021 tacagactct ttaatttcct gcttcacggt tttcagacgg ccgaagtacg ggaaatcatt
     1081 aactttcagg gctttagcgc ggttcagcag gtatgggata tcaaagttgg tagtgttgta
     1141 gccgatgata acgtccgggt ccactttgat gatgaaatta cgccagttgc tcagcatttc
     1201 ctcttcagtg gcgtgggaga aaatcataga accggtaatc ggagagcagg tgttcagggt
     1261 gaaaacatta cggatgaatg gtttcttggc acccgcgatg ctcacaacgt ttgcgatctg
     1321 aatcaccggg tcgtactctg gctccgggaa cacgccgatg cgaccggcac attcgatatc
     1381 aaaagacatg atacgcagcg gtgccgtatg ggaccagtca ccttcagccg ggtgagcgat
     1441 cagattacgg tagttaatgg aaacttccag ctgacaggag gagacgcggt tgttcggttc
     1501 aatcatgcta tatttaccct tcggcagggt gatccaggac atacccacga tgccgcagtc
     1561 caccatcaga cgcagggtgt acgcaatatt atcataggta gtggtgccgt tgctgaacca
     1621 gctgttgaag ctcaggtggc cgcgctcgaa cgcagtgcgc agtttgttta ccatgtgcgg
     1681 gtaggtaaca tagattttcc agaacggcag tttagtgtcg ccgctatagc cccaaatgga
     1741 ctgtttgctg acaacttcga tgctgtcaat agcgtgatcg aaggtttcgt tcagatagtg
     1801 aacgaacttg ttaatctgtt cttggtcgtt tgcgtcggag gaattcggag ccggaacgta
     1861 cagatagttt ttgaaaccag tcacgttgca cagaacggag tgaccttcag aagtcacgcc
     1921 gaagaagcgt acgacagtgg aggtgttctc gtctttgatg ccgttcagaa cggactgttc
     1981 agcatcaatc tgctgaaagg agatgtcgta cagggacggg tcgaaatcgg tcggcagctt
     2041 tttacgttcg aaagaagaca ggtcatgttc ttcctggtca gccatgtcat gttccatctg
     2101 ggacagctct tgttcgaacg tggattccag ctgggtaccc atcaggtcag tgtctttagc
     2161 tttgaaacct tgggagttat acttgcggaa ggagtcagac gggatgattt cgatggtgct
     2221 gaccggctca gaaccaacgc cgtggtcgat ggactggcgt ttaattttct tttccagctg
     2281 tggagtatct tcatcatcga ttttaacatc caccatgggc agggagcgtt tttcagacat
     2341 atgactagtg gatccggggt tttttctcct tgacgttaaa gtatagaggt atattaacaa
     2401 ttttttgttg atacttttat tacatttgaa taagaagtaa tacaaaccga aaatgttgaa
     2461 agtattagtt aaagtggtta tgcagttttt gcatttatat atctgttaat agatcaaaaa
     2521 tcatcgcttc gctgattaat taccccagaa ataaggctaa aaaactaatc gcattatcat
     2581 cctatggttg ttaatttgat tcgttcattt gaaggtttgt ggggccaggt tactgccaat
     2641 ttttcctctt cataaccata aaagctagta ttgtagaatc tttattgttc ggagcagtgc
     2701 ggcgcgaggc acatctgcgt ttcaggaacg cgaccggtga agacgaggac gcacggagga
     2761 gagtcttcct tcggagggct gtcacccgct cggcggcttc taatccgtac ttcaatatag
     2821 caatgagcag ttaagcgtat tactgaaagt tccaaagaga aggttttttt aggctaagat
     2881 aatggggctc tttacatttc cacaacatat aagtaagatt agatatggat atgtatatgg
     2941 atatgtatat ggtggtaatg ccatgtaata tgattattaa acttctttgc gtccatccaa
     3001 aaaaaaagta agaatttttg aaaattcaag gaattcgata tcaagcttat cgataccgtc
     3061 gacctcgagg gggggcccgg tacccaattc gccctatagt gagtcgtatt acgcgcgctc
     3121 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     3181 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     3241 cccttcccaa cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta
     3301 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     3361 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     3421 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     3481 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     3541 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     3601 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     3661 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     3721 acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     3781 gcatatcgac cggtcgagga gaacttctag tatatctaca tacctaatat tattgcctta
     3841 ttaaaaatgg aatcccaaca attacatcaa aatccacatt ctcttcaaaa tcaattgtcc
     3901 tgtacttcct tgttcatgtg tgttcaaaaa cgttatattt ataggataat tatactctat
     3961 ttctcaacaa gtaattggtt gtttggccga gcggtctaag gcgcctgatt caagaaatat
     4021 cttgaccgca gttaactgtg ggaatactca ggtatcgtaa gatgcaagag ttcgaatctc
     4081 ttagcaacca ttattttttt cctcaacata acgagaacac acaggggcgc tatcgcacag
     4141 aatcaaattc gatgactgga aattttttgt taatttcaga ggtcgcctga cgcatatacc
     4201 tttttcaact gaaaaattgg gagaaaaagg aaaggtgaga gcgccggaac cggcttttca
     4261 tatagaatag agaagcgttc atgactaaat gcttgcatca caatacttga agttgacaat
     4321 attatttaag gacctattgt tttttccaat aggtggttag caatcgtctt actttctaac
     4381 ttttcttacc ttttacattt cagcaatata tatatatata tttcaaggat ataccattct
     4441 aatgtctgcc cctaagaaga tcgtcgtttt gccaggtgac cacgttggtc aagaaatcac
     4501 agccgaagcc attaaggttc ttaaagctat ttctgatgtt cgttccaatg tcaagttcga
     4561 tttcgaaaat catttaattg gtggtgctgc tatcgatgct acaggtgttc cacttccaga
     4621 tgaggcgctg gaagcctcca agaaggctga tgccgttttg ttaggtgctg tgggtggtcc
     4681 taaatggggt accggtagtg ttagacctga acaaggttta ctaaaaatcc gtaaagaact
     4741 tcaattgtac gccaacttaa gaccatgtaa ctttgcatcc gactctcttt tagacttatc
     4801 tccaatcaag ccacaatttg ctaaaggtac tgacttcgtt gttgtcagag aattagtggg
     4861 aggtatttac tttggtaaga gaaaggaaga cgatggtgat ggtgtcgctt gggatagtga
     4921 acaatacacc gttccagaag tgcaaagaat cacaagaatg gccgctttca tggccctaca
     4981 acatgagcca ccattgccta tttggtcctt ggataaagct aatgttttgg cctcttcaag
     5041 attatggaga aaaactgtgg aggaaaccat caagaacgaa ttccctacat tgaaggttca
     5101 acatcaattg attgattctg ccgccatgat cctagttaag aacccaaccc acctaaatgg
     5161 tattataatc accagcaaca tgtttggtga tatcatctcc gatgaagcct ccgttatccc
     5221 aggttccttg ggtttgttgc catctgcgtc cttggcctct ttgccagaca agaacaccgc
     5281 atttggtttg tacgaaccat gccacggttc tgctccagat ttgccaaaga ataaggtcaa
     5341 ccctatcgcc actatcttgt ctgctgcaat gatgttgaaa ttgtcattga acttgcctga
     5401 agaaggtaag gccattgaag atgcagttaa aaaggttttg gatgcaggta tcagaactgg
     5461 tgatttaggt ggttccaaca gtaccaccga agtcggtgat gctgtcgccg aagaagttaa
     5521 gaaaatcctt gcttaaaaag attctctttt tttatgatat ttgtacataa actttataaa
     5581 tgaaattcat aatagaaacg acacgaaatt acaaaatgga atatgttcat agggtagacg
     5641 aaactatata cgcaatctac atacatttat caagaaggag aaaaaggagg atgtaaagga
     5701 atacaggtaa gcaaattgat actaatggct caacgtgata aggaaaaaga attgcacttt
     5761 aacattaata ttgacaagga ggagggcacc acacaaaaag ttaggtgtaa cagaaaatca
     5821 tgaaactatg attcctaatt tatatattgg aggattttct ctaaaaaaaa aaaaatacaa
     5881 caaataaaaa acactcaatg acctgaccat ttgatggagt ttaagtcaat accttcttga
     5941 accatttccc ataatggtga aagttccctc aagaatttta ctctgtcaga aacggcctta
     6001 acgacgtagt cgatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     6061 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     6121 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     6181 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     6241 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     6301 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     6361 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     6421 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     6481 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     6541 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     6601 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     6661 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     6721 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     6781 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     6841 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     6901 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     6961 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     7021 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     7081 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     7141 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     7201 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     7261 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     7321 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     7381 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     7441 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     7501 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     7561 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     7621 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     7681 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     7741 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     7801 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     7861 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     7921 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     7981 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     8041 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     8101 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     8161 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     8221 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     8281 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     8341 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     8401 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     8461 gaacaaaagc tggagctcca ccgcggtggc ggccgctcag cagatcctta gtcatcgtca
     8521 tccttgtaat cgatgtcatg atctttataa tcaccgtcat ggtctttgta gtcacttcca
     8581 gaccatttgg acagctgttc tactttctct tgcagttcct ttttgacttt aacacgcata
     8641 tagaagatat cgcagttttt gttgctacac agcacttcgc tgtgcaggtt gcccgcgcaa
     8701 cgctggcact gggtccacag acgggaatat ttctcctcca gatcacgtac gtcgtacagt
     8761 gccttgatgt acagttcacc ggagcgcgcc agacagttag agcacagcgg gccctcgccc
     8821 ttgcgcagcg gacctttaca agatttacac gcttctacct ttttaatgaa ggacatcaga
     8881 ccacctttct ggctaccggt gttgatctta atagatttaa caacaaacat accatttgct
     8941 tgtttgtcac cgatgatcgg cgccacgatg ctgataatcg ggttctgcag ttggttagtc
     9001 aggtaatagc ggctgtctac ctggatgttg ttctccagta caaacagtgg atcttctgcg
     9061 cggttgtaca gtttgtcgtt gccgccaata ataacatagt caacgcggtc acccacgttc
     9121 gggcccacac cttcacggcg tttcatacgt tcagccagta ctgcgtgtgg ctgcgggttg
     9181 gtgtagttcg gcgccagggt tttgctgatg atcagtttgc tgatatcaac acggttatgc
     9241 agaatgtcat tgatggtttc acgaacaaaa gccagagcgc cgtcgacgtt gcgctcgatc
     9301 agaatctttt tcagaacttt gttcataacg atggaaacca gggagcagct gtcgcgacgt
     9361 acagatgcca ggcccttttg atccagcttg tcgaatttat ccgggttcgt ccagaacaga
     9421 ccagcgtagc gtttcttgtt gatcagcagg tacgggaagt aagccttttc aaactccaga
     9481 ttgatcgggt gcttgaacag cgtggaaaca tatttggcag cctcagtacc cag
//
