LOCUS       sequence-474678-       12048 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express SmBiT-NCOR2 Fusion Protein in Mammalian Cells under a CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12048)
  AUTHORS   Promega
  TITLE     Promega Plasmid Collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: SmBiT-NCOR2 Vector
FEATURES             Location/Qualifiers
     source          1..12048
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    2165..2286
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2176..2195)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(2401..2434)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2444..2491
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2523..3317
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2577..2596)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3187..3206
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      3534..4079
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     3980..3999
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4196..4213
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    4363..4411
                     /note="synthetic polyadenylation signal"
     CDS             complement(4435..4833)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      4830..4839
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4864..5221)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4878..5013
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        5030..5221
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     5178..5198
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    5300..5381
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        5614..5917
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5918..6121
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6072..6092
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          6257..6389
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     6372..6391
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     6433..6452
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6433..6451
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cagcgccaca gcagctcccc actctcccca ggaggtccaa cacacttgac aaaaccaacc
       61 accacgtcct cgtccgagcg ggagcgagac cgggatcgag agcgggaccg ggatcgggag
      121 cgggaaaagt ccatcctcac gtccaccacg acggtggagc acgcacccat ctggagacct
      181 ggtacagagc agagcagcgg cagcagcggc agcagcggcg ggggtggggg cagcagcagc
      241 cgccccgcct cccactccca tgcccaccag cactcgccca tctcccctcg gacccaggat
      301 gccctccagc agagacccag tgtgcttcac aacacaggca tgaagggtat catcaccgct
      361 gtggagccca gcacgcccac ggtcctgagg tccacctcca cctcctcacc cgttcgcccg
      421 gctgccacat tcccacctgc cacccactgc ccactgggcg gcaccctcga tggggtctac
      481 cctaccctca tggagcccgt cttgctgccc aaggaggccc cccgggtcgc ccggccagag
      541 cggccccgag cagacaccgg ccatgccttc ctcgccaagc ccccagcccg ctccgggctg
      601 gagcccgcct cctcccccag caagggctcg gagccccggc ccctagtgcc tcctgtctct
      661 ggccacgcca ccatcgcccg cacccctgcg aagaacctcg cacctcacca cgccagcccg
      721 gacccgccgg cgccacctgc ctcggcctcg gacccgcacc gggaaaagac tcaaagtaaa
      781 cccttttcca tccaggaact ggaactccgt tctctgggtt accacggcag cagctacagc
      841 cccgaagggg tggagcccgt cagccctgtg agctcaccca gtctgaccca cgacaagggg
      901 ctccccaagc acctggaaga gctcgacaag agccacctgg agggggagct gcggcccaag
      961 cagccaggcc ccgtgaagct tggcggggag gccgcccacc tcccacacct gcggccgctg
     1021 cctgagagcc agccctcgtc cagcccgctg ctccagaccg ccccaggggt caaaggtcac
     1081 cagcgggtgg tcaccctggc ccagcacatc agtgaggtca tcacacagga ctacacccgg
     1141 caccacccac agcagctcag cgcacccctg cccgcccccc tctactcctt ccctggggcc
     1201 agctgccccg tcctggacct ccgccgccca cccagtgacc tctacctccc gcccccggac
     1261 catggtgccc cggcccgtgg ctccccccac agcgaagggg gcaagaggtc tccagagcca
     1321 aacaagacgt cggtcttggg tggtggtgag gacggtattg aacctgtgtc cccaccggag
     1381 ggcatgacgg agccagggca ctcccggagt gctgtgtacc cgctgctgta ccgggatggg
     1441 gaacagacgg agcccagcag gatgggctcc aagtctccag gcaacaccag ccagccgcca
     1501 gccttcttca gcaagctgac cgagagcaac tccgccatgg tcaagtccaa gaagcaagag
     1561 atcaacaaga agctgaacac ccacaaccgg aatgagcctg aatacaatat cagccagcct
     1621 gggacggaga tcttcaatat gcccgccatc accggaacag gtggcggcgg gaaggccaag
     1681 gtctctggca gacccagcag ccgaaaagcc aagtccccgg ccccgggcct ggcatctggg
     1741 gaccggccac cctctgtctc ctcagtgcac tcggagggag actgcaaccg ccggacgccg
     1801 ctcaccaacc gcgtgtggga ggacaggccc tcgtccgcag gttccacgcc attcccctac
     1861 aaccccctga tcatgcggct gcaggcgggt gtcatggctt ccccaccccc accgggcctc
     1921 cccgcgggca gcgggcccct cgctggcccc caccacgcct gggacgagga gcccaagcca
     1981 ctgctctgct cgcagtacga gacactctcc gacagcgagg tttaaacgaa ttcgggctcg
     2041 gtacccgggg atcctctaga gtcgacctgc aggcatgcaa gctgatccgg ctgctaacaa
     2101 agcccgaaag gaagctgagt tggctgctgc caccgctgag caaggccgct tcgagcagac
     2161 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     2221 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     2281 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag
     2341 gtttttttaa gcaagtaaaa cctctacaaa tgtggtaaaa tcttaattaa gaattctaat
     2401 ataacttcgt atagcataca ttatacgaag ttatggatcc actgttgaca attaatcatc
     2461 ggcatagtat atcggcatag tataatacga caaggtgagg aactaaaccc aggaggcaga
     2521 tcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     2581 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     2641 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     2701 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     2761 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     2821 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     2881 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     2941 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     3001 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     3061 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     3121 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     3181 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     3241 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     3301 ttgacgagtt cttctgattc gaaatgaccg accaagcgac gcccaaccgg tatcagctca
     3361 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtc
     3421 aaacatgaga attacaactt atatcgtatg gggctgactt caggtgctac atttgaagag
     3481 ataaattgca ctgaaatcta gaaatatttt atctgattaa taagatgatc ttcttgagat
     3541 cgttttggtc tgcgcgtaat ctcttgctct gaaaacgaaa aaaccgcctt gcagggcggt
     3601 ttttcgaagg ttctctgagc taccaactct ttgaaccgag gtaactggct tggaggagcg
     3661 cagtcaccaa aacttgtcct ttcagtttag ccttaaccgg cgcatgactt caagactaac
     3721 tcctctaaat caattaccag tggctgctgc cagtggtgct tttgcatgtc tttccgggtt
     3781 ggactcaaga cgatagttac cggataaggc gcagcggtcg gactgaacgg ggggttcgtg
     3841 catacagtcc agcttggagc gaactgccta cccggaactg agtgtcaggc gtggaatgag
     3901 acaaacgcgg ccataacagc ggaatgacac cggtaaaccg aaaggcagga acaggagagc
     3961 gcacgaggga gccgccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4021 accactgatt tgagcgtcag atttcgtgat gcttgtcagg ggggcggagc ctatggaaaa
     4081 acggctttgc cgcggccctc tcacttccct gttaagtatc ttcctggcat cttccaggaa
     4141 atctccgccc cgttcgtaag ccatttccgc tcgccgcagt cgaacgaccg agcgtagcga
     4201 gtcagtgagc gaggaagcgg aatatatcct gtatcacata ttctgctgac gcaccggtgc
     4261 agcctttttt ctcctgccac atgaagcact tcactgacac cctcatcagt gccaacatag
     4321 taagccagta tacactccgc tagcgctgat gtccggaatc gtcacacaaa aaaccaacac
     4381 acagatgtaa tgaaaataaa gatattttat tgcggccatc gtgatggcta gagtttagcc
     4441 ctcccacaca taaccagagg gcagcaattc acgaatccca actgccgtcg gctgtccatc
     4501 actgtccttc actatggctt tgatcccagg atgcagatcg agaagcacct gtcggcaccg
     4561 tccgcagggg ctcaagatgc ccctgttctc atttccgatc gcgacgatac aagtcaggtt
     4621 gccagctgcc gcagcagcag cagtgcccag caccacgagt tctgcacaag gtcccccagt
     4681 aaaatgatat acattgacac cagtgaagat gcggccgtcg ctagagagag ctgcgctggc
     4741 gacgctgtag tcttcagaga tggggatgct gttgattgta gccgttgctc tttcaatgag
     4801 ggtggattct tcttgagaca aaggcttggc catggtggcg ctagtgtcag aagaatcgag
     4861 ctttttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga
     4921 ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga
     4981 atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt
     5041 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
     5101 tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     5161 gcctggggac tttccacacc ctaactgaca cacattccac agctggttct ttccgcctca
     5221 gaaggtacct aaccaagttc ctctttcaga ggttatttca ggccatggtg ctgcgcaaga
     5281 cgcgttaatt aagacgtcgg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     5341 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     5401 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
     5461 acaaatgtgg tatggctgat tatgatccgc ggccgcaata cgtcgacgtt atcagctgac
     5521 ttcgtacgag agcctaggat tatggcgcgc cactagttat taatagtaat caattacggg
     5581 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     5641 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     5701 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     5761 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
     5821 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
     5881 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
     5941 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     6001 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
     6061 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     6121 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
     6181 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
     6241 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
     6301 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
     6361 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     6421 aggctagagt attaatacga ctcactatag ggctagcgct caccatggtg accggctacc
     6481 ggctgttcga ggagattctc gggagctccg gtggtggcgg gagcggaggt ggaggctcga
     6541 gcggtgcgat cgccatgtcg ggatccacac agcctgtggc acagacgtgg agggccactg
     6601 agccccgcta cccgccccac agcctttcct acccagtgca gatcgcccgg acgcacacgg
     6661 acgtcgggct cctggagtac cagcaccact cccgcgacta tgcctcccac ctgtcgcccg
     6721 gctccatcat ccagccccag cggcggaggc cctccctgct gtctgagttc cagcccggga
     6781 atgaacggtc ccaggagctc cacctgcggc cagagtccca ctcatacctg cccgagctgg
     6841 ggaagtcaga gatggagttc attgaaagca agcgccctcg gctagagctg ctgcctgacc
     6901 ccctgctgcg accgtcaccc ctgctggcca cgggccagcc tgcgggatct gaagacctca
     6961 ccaaggaccg tagcctgacg ggcaagctgg aaccggtgtc tccccccagc cccccgcaca
     7021 ctgaccctga gctggagctg gtgccgccac ggctgtccaa ggaggagctg atccagaaca
     7081 tggaccgcgt ggaccgagag atcaccatgg tagagcagca gatctctaag ctgaagaaga
     7141 agcagcaaca gctggaggag gaggctgcca agccgcccga gcctgagaag cccgtgtcac
     7201 cgccgcccat cgagtcgaag caccgcagcc tggtgcagat catctacgac gagaaccgga
     7261 agaaggctga agctgcacat cggattctgg aaggcctggg gccccaggtg gagctgccgc
     7321 tgtacaacca gccctccgac acccggcagt atcatgagaa catcaaaata aaccaggcga
     7381 tgcggaagaa gctaatcttg tacttcaaga ggaggaatca cgctcggaaa caatgggagc
     7441 agaagttctg ccagcgctat gaccagctca tggaggcctg ggagaagaag gtggagcgca
     7501 tcgagaacaa cccccggcgg cgggccaagg agagcaaggt gcgcgagtac tacgagaagc
     7561 agttccctga gatccgcaag cagcgcgagc tgcaggagcg catgcagagc agggtgggcc
     7621 agcggggcag tgggctgtcc atgtcggccg cccgcagcga gcacgaggtg tcagagatca
     7681 tcgatggcct ctcagagcag gagaacctgg agaagcagat gcgccagctg gccgtgatcc
     7741 cgcccatgct gtacgacgct gaccagcagc gcatcaagtt catcaacatg aacgggctta
     7801 tggccgaccc catgaaggtg tacaaagacc gccaggtcat gaacatgtgg agtgagcagg
     7861 agaaggagac cttccgggag aagttcatgc agcatcccaa gaactttggc ctgatcgcat
     7921 cattcctgga gaggaagaca gtggctgagt gcgtcctcta ttactacctg actaagaaga
     7981 atgagaacta taagagcctg gtgagacgga gctatcggcg ccgcggcaag agccagcagc
     8041 agcaacaaca gcagcagcag cagcagcagc agcagcagca gcagcccatg ccccgcagca
     8101 gccaggagga gaaagatgag aaggagaagg aaaaggaggc ggagaaggag gaggagaagc
     8161 cggaggtgga gaacgacaag gaagacctcc tcaaggagaa gacagacgac acctcagggg
     8221 aggacaacga cgagaaggag gctgtggcct ccaaaggccg caaaactgcc aacagccagg
     8281 gaagacgcaa aggccgcatc acccgctcaa tggctaatga ggccaacagc gaggaggcca
     8341 tcacccccca gcagagcgcc gagctggcct ccatggagct gaatgagagt tctcgctgga
     8401 cagaagaaga aatggaaaca gccaagaaag gtctcctgga acacggccgc aactggtcgg
     8461 ccatcgcccg gatggtgggc tccaagactg tgtcgcagtg taagaacttc tacttcaact
     8521 acaagaagag gcagaacctc gatgagatct tgcagcagca caagctgaag atggagaagg
     8581 agaggaacgc gcggaggaag aagaagaaag cgccggcggc ggccagcgag gaggctgcat
     8641 tcccgcccgt ggtggaggat gaggagatgg aggcgtcggg cgtgagcgga aatgaggagg
     8701 agatggtgga ggaggctgaa gccttacatg cctctgggaa tgaggtgccc agaggggaat
     8761 gcagtggccc agccactgtc aacaacagct cagacaccga gagcatcccc tctcctcaca
     8821 ctgaggccgc caaggacaca gggcagaatg ggcccaagcc cccagccacc ctgggcgccg
     8881 acgggccacc cccagagcca cccaccccac caccggagga catcccggcc cccactgagc
     8941 ccaccccggc ctctgaagcc accggagccc ctacgccccc accagcaccc ccatcgccct
     9001 ctgcacctcc tcctgtggtc cccaaggagg agaaggagga ggagaccgca gcagcgcccc
     9061 cagtggagga gggggaggag cagaagcccc ccgcggctga ggagctggca gtggacacag
     9121 ggaaggccga ggagcccgtc aagagcgagt gcacggagga agccgaggag gggccggcca
     9181 agggcaagga cgcggaggcc gctgaggcca cggccgaggg ggcgctcaag gcagagaaga
     9241 aggagggcgg gagcggcagg gccaccacag ccaagagctc gggcgccccc caggacagcg
     9301 actccagtgc cacctgcagt gcagacgagg tggatgaggc cgagggcggc gacaagaacc
     9361 ggctgctgtc cccaaggccc agcctcctca ccccgactgg cgacccccgg gccaatgcct
     9421 caccccagaa gccactggac ctgaagcagc tgaagcagcg agcggctgcc atccccccca
     9481 tccaggtcac caaagtccat gagccccccc gggaggacgc agctcccacc aagccagctc
     9541 ccccagcccc accgccaccg caaaacctgc agccggagag cgacgcccct cagcagcctg
     9601 gcagcagccc ccggggcaag agcaggagcc cggcaccccc cgccgacaag gaggcagaga
     9661 agcctgtgtt cttcccagcc ttcgcagccg aggcccagaa gctgcctggg gacccccctt
     9721 gctggacttc cggcctgccc ttccccgtgc ccccccgtga ggtgatcaag gcctccccgc
     9781 atgccccgga cccctcagcc ttctcctacg ctccacctgg tcacccactg cccctgggcc
     9841 tccatgacac tgcccggccc gtcctgccgc gcccacccac catctccaac ccgcctcccc
     9901 tcatctcctc tgccaagcac cccagcgtcc tcgagaggca aataggtgcc atctcccaag
     9961 gaatgtcggt ccagctccac gtcccgtact cagagcatgc caaggccccg gtgggccctg
    10021 tcaccatggg gctgcccctg cccatggacc ccaaaaagct ggcacccttc agcggagtga
    10081 agcaggagca gctgtcccca cggggccagg ctgggccacc ggagagcctg ggggtgccca
    10141 cagcccagga ggcgtccgtg ctgagaggga cagctctggg ctcagttccg ggcggaagca
    10201 tcaccaaagg cattcccagc acacgggtgc cctcggacag cgccatcaca taccgcggct
    10261 ccatcaccca cggcacgcca gctgacgtcc tgtacaaggg caccatcacc aggatcatcg
    10321 gcgaggacag cccgagtcgc ttggaccgcg gccgggagga cagcctgccc aagggccacg
    10381 tcatctacga aggcaagaag ggccacgtct tgtcctatga gggtggcatg tctgtgaccc
    10441 agtgctccaa ggaggacggc agaagcagct caggaccccc ccatgagacg gccgccccca
    10501 agcgcaccta tgacatgatg gagggccgcg tgggcagagc catctcctca gccagcatcg
    10561 aaggtctcat gggccgtgcc atcccgccgg agcgacacag cccccaccac ctcaaagagc
    10621 agcaccacat ccgcgggtcc atcacacaag ggatccctcg gtcctacgtg gaggcacagg
    10681 aggactacct gcgtcgggag gccaagctcc taaagcggga gggcacgcct ccgcccccac
    10741 cgccctcacg ggacctgacc gaggcctaca agacgcaggc cctgggcccc ctgaagctga
    10801 agccggccca tgagggcctg gtggccacgg tgaaggaggc gggccgctcc atccatgaga
    10861 tcccgcgcga ggagctgcgg cacacgcccg agctgcccct ggccccgcgg ccgctcaagg
    10921 agggctccat cacgcagggc accccgctca agtacgacac cggcgcgtcc accactggct
    10981 ccaaaaagca cgacgtacgc tccctcatcg gcagccccgg ccggacgttc ccacccgtgc
    11041 acccgctgga tgtgatggcc gacgcccggg cactggaacg tgcctgctac gaggagagcc
    11101 tgaagagccg gccagggacc gccagcagct cggggggctc cattgcgcgc ggcgccccgg
    11161 tcattgtgcc tgagctgggc aagccgcggc agagccccct aacctatgag gaccacgggg
    11221 caccctttgc cggccacctc ccacgaggtt cgcccgtgac cacgcgggag cccacgccgc
    11281 gcctgcagga gggcagcctt tcgtccagca aggcatccca ggaccgaaag ctgacgtcga
    11341 cgcctcgtga gatcgccaag tccccgcaca gcaccgtgcc cgagcaccac ccacacccca
    11401 tctcgcccta tgagcacctg cttcggggcg tgagtggcgt ggacctgtat cgcagccaca
    11461 tccccctggc cttcgacccc acctccatac cccgcggcat ccctctggac gcagccgctg
    11521 cctactacct gccccgacac ctggccccca accccaccta cccgcacctg tacccaccct
    11581 acctcatccg cggctacccc gacacggcgg cgctggagaa ccggcagacc atcatcaatg
    11641 actacatcac ctcgcagcag atgcaccaca acgcggccac cgccatggcc cagcgagctg
    11701 atatgctgag gggcctctcg ccccgcgagt cctcgctggc actcaactac gctgcgggtc
    11761 cccgaggcat catcgacctg tcccaagtgc cacacctgcc tgtgctcgtg cccccgacac
    11821 caggcacccc agccaccgcc atggaccgcc ttgcctacct ccccaccgcg ccccagccct
    11881 tcagcagccg ccacagcagc tccccactct ccccaggagg tccaacacac ttgacaaaac
    11941 caaccaccac gtcctcgtcc gagcgggagc gagaccggga tcgagagcgg gaccgggatc
    12001 gggagcggga aaagtccatc ctcacgtcca ccacgacggt ggagcacc
//
