LOCUS       sequence-474728-        9525 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Co-expressing membrane-localized Voltron2 and soma-localized 
            CheRiff..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Wong-Campos JD, Park P, Davis H, Qi Y, Tian H, Itkis DG, Kim D, 
            Grimm JB, Plutkis SE, Lavis L, Cohen AE
  TITLE     Voltage dynamics of dendritic integration and back-propagation in 
            vivo.
  JOURNAL   bioRxiv [Preprint]. 2023 May 26:2023.05.25.542363. doi: 
            10.1101/2023.05.25.542363.
  PUBMED    37292691
REFERENCE   2  (bases 1 to 9525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2023 May 26:2023.05.25.542363. doi: 
            10.1101/2023.05.25.542363."
COMMENT     Sequence Label: PP_079_pAAV_CAG_LR-Voltron2-P2A-CheRiff-eGFP-S...
FEATURES             Location/Qualifiers
     source          1..9525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     enhancer        223..526
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        528..802
                     /label=chicken beta-actin promoter
     intron          803..1819
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1743..1765
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1827..1846
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1908..1917
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1980..2018
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
     CDS             3699..3755
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3756..4700
                     /codon_start=1
                     /product="blue light-activated optogenetic actuator derived
                     from Scherffelia dubia channelrhodopsin (Hochbaum et al., 
                     2014)"
                     /label=CheRiff
                     /translation="GGAPAPDAHSAPPGNDSAGGSEYHAPAGYQVNPPYHPVHGYEEQC
                     SSIYIYYGALWEQETARGFQWFAVFLSALFLAFYGWHAYKASVGWEEVYVCSVELIKVI
                     LEIYFEFTSPAMLFLYGGNITPWLRYAEWLLTCPVILIHLSNITGLSEAYNKRTMALLV
                     SDLGTICMGVTAALATGWVKWLFYCIGLVYGTQTFYNAGIIYVESYYIMPAGGCKKLVL
                     AMTAVYYSSWLMFPGLFIFGPEGMHTLSVAGSTIGHTIADLLSKNIWGLLGHFLRIKIH
                     EHIIMYGDIRRPVSSQFLGRKVDVLAFVTEEDKV"
     CDS             join(4716..4718,4719..5429)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(4716..4718,4719..5429)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4758..4779)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5019..5038)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5366..5387
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5652..6240
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5705..5725)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6123..6134)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6272..6748
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(6471..6490)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   6788..6928
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6788..6917
                     /label=AAV2 ITR
     rep_origin      7003..7458
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7090..7109)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7300..7321
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7475..7494)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7594..7616
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7654..7672)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7740..7844
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7845..7913,7914..8705)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8063..8082)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8876..9464
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9365..9384
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtccagtt tggttaatta actagttatt aatagtaatc
      181 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      241 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      301 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      361 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      421 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      481 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc
      541 cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt
      601 tattttttaa ttattttgtg cagcgatggg ggcggggggg ggggggcgcg cgccaggcgg
      661 ggsggggsgg ggggaggggg ggggsggggs gaggsggaga ggtgcggcgg cagccaatca
      721 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa
      781 aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc
      841 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag
      901 cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt
      961 ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg
     1021 gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc
     1081 gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt
     1141 gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa
     1201 caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt
     1261 cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg
     1321 tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca
     1381 ggkgggggtg ccgggcgggg cggggccgcc kcgggccggg grgggctcgg gggrggggcg
     1441 cggcggtccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt
     1501 atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa
     1561 atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg
     1621 caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc
     1681 tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc
     1741 ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc
     1801 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt
     1861 tggcaaagaa ttgatttgat accgcgggcc cgggatctcg aggatccgcc accatgggcc
     1921 gaccccaaat actcctcttg ctggctttgt tgacccttgg actggctaac ggaacagaag
     1981 gtcccaactt ctacgttcct ttcagcaata agacaggcgt agtcagatcc ggtagtgctg
     2041 acgtggaaac cgagaccggc atgattgcac agtggattgt ctttgctatt atggctgctg
     2101 ctgctattgc ttttggagtg gctgtgcact ttcggccttc agagctgaag agcgcatact
     2161 atatcaacat tgccatctgc actatcgccg ctaccgctta ctatgcaatg gccgtgaact
     2221 accaggacct gacaatgaat ggtgaaaggc aggtggtcta cgcaagatat attaactggg
     2281 tgctgaccac accactgctc ctgctcgatc tcatcgtcat gaccaagatg ggcggagtga
     2341 tgatttcttg ggtcatcggc gcagacattt tcatgatcgt gtttggtatt ctgggcgact
     2401 tcgaggatga acacaagttc aaatgggtgt actttatcgc tggatgtgtg atgcaggcag
     2461 tcctgacata cgggatgtat aacgccactt ggaaagacga tctgaagaaa agccccgagt
     2521 accatagctc ctatgtcagt ctgctcgtct tcctgtcaat cctctgggtg ttttatcctg
     2581 tcgtgtgggc tttcgggtct ggtagtggcg tgctgtccgt cgacaatgag gccattctca
     2641 tgggaatcct ggatgtgctc gctaagccac tgtttggaat ggggtgcctc attgcccatg
     2701 agactatctt caagatcggt actggctttc cattcgaccc ccattatgtg gaagtcctgg
     2761 gcgagcgcat gcactacgtc gatgttggtc cgcgcgatgg cacccctgtg ctgttcctgc
     2821 acggtaaccc gacctcctcc tacgtgtggc gcaacatcat cccgcatgtt gcaccgaccc
     2881 atcgctgcat tgctccagac ctgatcggta tgggcaaatc cgacaaacca gacctgggtt
     2941 atttcttcga cgaccacgtc cgcttcatgg atgccttcat cgaagccctg ggtctggaag
     3001 aggtcgtcct ggtcattcac gactggggct ccgctctggg tttccactgg gccaagcgca
     3061 atccagagcg cgtcaaaggt attgcattta tggagttcat ccgccctatc ccgacctggg
     3121 acgaatggcc agaatttgcc cgcgagacct tccaggcctt ccgcaccacc gacgtcggcc
     3181 gcaagctgat catcgatcag aacgttttta tcgagggtac gctgccgatg ggtgtcgtcc
     3241 gcccgctgac tgaagtcgag atggaccatt accgcgagcc gttcctgaat cctgttgacc
     3301 gcgagccact gtggcgcttc ccaaacgagc tgccaatcgc cggtgagcca gcgaacatcg
     3361 tcgcgctggt cgaagaatac atggactggc tgcaccagtc ccctgtcccg aagctgctgt
     3421 tctggggcac cccaggcgtt ctgatcccac cggccgaagc cgctcgcctg gccaaaagcc
     3481 tgcctaactg caaggctgtg gacatcggcc cgggtctgaa tctgctgcaa gaagacaacc
     3541 cggacctgat cggcagcgag atcgcgcgct ggctgtcgac gctcgagatt tccggcgagc
     3601 caaccactaa gagcaggatc accagcgagg gcgagtacat ccccctggac cagatcgaca
     3661 tcaacgtgtt ctgctacgag aacgaggtgg gctccggagc cacgaacttc tctctgttaa
     3721 agcaagcagg agacgtggaa gaaaaccccg gtcccggcgg agctcctgct ccagacgctc
     3781 acagcgcccc acctggaaac gattctgccg gaggcagtga gtaccatgcc ccagctggat
     3841 atcaagtgaa tccaccctac caccccgtgc atgggtatga ggaacagtgc agctccatct
     3901 acatctacta tggggccctg tgggagcagg aaacagctag gggcttccag tggtttgccg
     3961 tgttcctgtc tgccctgttt ctggctttct acggctggca cgcctataag gccagcgtgg
     4021 gatgggagga agtgtacgtg tgctccgtgg agctgatcaa agtgattctg gagatctatt
     4081 tcgagttcac cagtcctgct atgctgttcc tgtacggagg gaacattacc ccatggctga
     4141 gatatgccga atggctgctg acatgtcccg tgatcctgat tcatctgtct aacatcaccg
     4201 gcctgagtga ggcatacaat aagcggacaa tggctctgct ggtgtccgac ctgggaacta
     4261 tttgcatggg agtgacagcc gctctggcca ctgggtgggt gaagtggctg ttttactgta
     4321 tcggcctggt gtatggaacc cagacattct acaacgctgg aatcatctac gtggagtctt
     4381 actatatcat gcctgccggc ggctgtaaga aactggtgct ggccatgact gccgtgtact
     4441 attctagttg gctgatgttt cccggcctgt tcatctttgg gcctgaaggc atgcacaccc
     4501 tgagcgtggc tgggtccact attggccata ccatcgccga cctgctgtcc aagaatattt
     4561 ggggactgct ggggcacttc ctgcggatca aaattcacga gcatatcatt atgtacggcg
     4621 atatcaggag accagtgagc tcccagtttc tgggacgcaa ggtggacgtg ctggccttcg
     4681 tgacagagga agataaagtg gcggccgcca agagcgtgag caagggcgag gagctgttca
     4741 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     4801 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatttgca
     4861 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     4921 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     4981 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     5041 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     5101 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     5161 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     5221 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     5281 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     5341 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     5401 tcactctcgg catggacgag ctgtacaagc agtcccagcc catcctcaac accaaggaga
     5461 tggccccgca gagcaagcct ccagaggagc tggagatgag cagcatgccc agccccgtgg
     5521 cccctctgcc cgcacgcacg gagggcgtca tcgacatgcg gagcatgtcc agcattgaca
     5581 gcttcatcag ctgtgccacg gacttccctg aagccaccag attctaagaa ttcgatatca
     5641 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     5701 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     5761 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     5821 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     5881 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     5941 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     6001 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     6061 cttggctgct cgcctatgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     6121 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     6181 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     6241 atcgataccg agcgctgctc gagagatcta cgggtggcat ccctgtgacc cctccccagt
     6301 gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt
     6361 aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt
     6421 ggtatggagc aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga
     6481 accaagctgg agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca
     6541 agcgattctc ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct
     6601 cagctaattt ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc
     6661 caactcctaa tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc
     6721 gtgaaccact gctcccttcc ctgtccttct gattttgtag gtaaccacgt gcggaccgag
     6781 cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
     6841 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
     6901 agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat
     6961 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
     7021 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct
     7081 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     7141 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     7201 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     7261 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     7321 gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
     7381 cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     7441 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     7501 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     7561 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     7621 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     7681 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     7741 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     7801 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     7861 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     7921 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     7981 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     8041 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     8101 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     8161 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     8221 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     8281 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     8341 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     8401 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     8461 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     8521 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     8581 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     8641 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     8701 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     8761 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     8821 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     8881 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     8941 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     9001 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     9061 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     9121 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     9181 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     9241 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     9301 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     9361 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     9421 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     9481 cctttttacg gttcctggcc ttttgctggc cttttgctca catgt
//
