LOCUS       sequence-474741-       11632 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses PYL1-G3BP1 for recruiting RNA to the stress granule via 
            CRISPR-TO.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11632)
  AUTHORS   Han M, Fu ML, Zhu Y, Choi AA, Li E, Bezney J, Cai S, Miles L, Ma Y, 
            Qi LS
  TITLE     Programmable control of spatial transcriptome in live cells and 
            neurons.
  JOURNAL   Nature. 2025 Jul;643(8070):241-251. doi: 10.1038/s41586-025-09020-z.
            Epub 2025 May 21.
  PUBMED    40399675
REFERENCE   2  (bases 1 to 11632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-025-09020-z"; journalName: "Nature"; date: 
            "2025-07"; volume: "643"; issue: "8070"; pages: "241-251"
COMMENT     Sequence Label: pSLQ10552
FEATURES             Location/Qualifiers
     source          1..11632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    69..194
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    691..924
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1109..1153
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1438..1555
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1620..2119
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1655..1678)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2052..2071
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2735..3430
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     misc_feature    4884..5472
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4937..4957)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5355..5366)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5475..5491)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5476..5492)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5582..5815
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5840..5858)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5841..5858)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6148..6167)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6381..6400)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6500..6522
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6560..6578)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6646..6750
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6751..6819,6820..7611)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6969..6988)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7782..8370
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8271..8290
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8524..8541
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8611..8631)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8616..8945
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8796..8931
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10079..10144
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10274..10294
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10719..10853
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10756..10775)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10810..10829
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(11021..11632,1..22)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
       61 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
      121 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
      181 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
      241 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
      301 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
      361 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
      421 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
      481 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc
      541 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
      601 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
      661 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
      721 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
      781 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
      841 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
      901 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
      961 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     1021 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     1081 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     1141 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     1201 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     1261 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     1321 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     1381 cattcgatta gtgaacggat ctcgacggta tcgccaaatg gcagtattca tccacaattt
     1441 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1501 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     1561 ttattacagg gacagcagag atccagtttg gatcgataag cttgatatcg aattctaccg
     1621 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     1681 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
     1741 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
     1801 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
     1861 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
     1921 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
     1981 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
     2041 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
     2101 ctcatctccg ggcctttcga cctgcagccc aagcttacgc gtctgcagga tatcaagctt
     2161 gcggtacgac tgagtcatgg gtgggggcgc gccaactcaa gacgaattca cccaactctc
     2221 ccaatcaatc gccgagttcc acacgtacca actcggtaac ggccgttgct catctctcct
     2281 agctcagcga atccacgcgc cgccggaaac agtatggtcc gtggtgagac gtttcgatag
     2341 gccacagatt tacaaacact tcatcaaaag ctgtaacgtg agtgaagatt tcgagatgcg
     2401 agtgggatgc acgcgcgacg tgaacgtgat aagtggatta ccggcgaata cgtctcgaga
     2461 gagattagat ctgttggacg atgatcggag agtgactggg tttagtataa ccggtggtga
     2521 acataggctg aggaattata aatcggttac gacggttcat agatttgaga aagaagaaga
     2581 agaagaaagg atctggaccg ttgttttgga atcttatgtt gttgatgtac cggaaggtaa
     2641 ttcggaggaa gatacgagat tgtttgctga tacggttatt agattgaatc ttcagaaact
     2701 tgcttcgatc actgaagcta tgaacgtgga tccgagcgag ctgattaagg agaacatgca
     2761 catgaagctg tacatggagg gcaccgtgga caaccatcac ttcaagtgca catccgaggg
     2821 cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggtggtcg agggcggccc
     2881 tctccccttc gccttcgaca tcctggctac tagcttcctc tacggcagca agaccttcat
     2941 caaccacacc cagggcatcc ccgacttctt caagcagtcc ttccctgagg gcttcacatg
     3001 ggagagagtc accacatacg aagacggggg cgtgctgacc gctacccagg acaccagcct
     3061 ccaggacggc tgcctcatct acaacgtcaa gatcagaggg gtgaacttca catccaacgg
     3121 ccctgtgatg cagaagaaaa cactcggctg ggaggccttc accgagacgc tgtaccccgc
     3181 tgacggcggc ctggaaggca gaaacgacat ggccctgaag ctcgtgggcg ggagccatct
     3241 gatcgcaaac atcaagacca catatagatc caagaaaccc gctaagaacc tcaagatgcc
     3301 tggcgtctac tatgtggact acagactgga aagaatcaag gaggccaaca acgagaccta
     3361 cgtcgagcag cacgaggtgg cagtggccag atactgcgac ctccctagca aactggggca
     3421 caagcttaat cggaccgcaa gcaagatggt gatggagaag cctagtcccc tgctggtcgg
     3481 gcgggaattt gtgagacagt attacacact gctgaaccag gccccagaca tgctgcatag
     3541 attttatgga aagaactctt cttatgtcca tgggggattg gattcaaatg gaaagccagc
     3601 agatgcagtc tacggacaga aagaaatcca caggaaagtg atgtcacaaa acttcaccaa
     3661 ctgccacacc aagattcgcc atgttgatgc tcatgccacg ctaaatgatg gtgtggtagt
     3721 ccaggtgatg gggcttctct ctaacaacaa ccaggctttg aggagattca tgcaaacgtt
     3781 tgtccttgct cctgaggggt ctgttgcaaa taaattctat gttcacaatg atatcttcag
     3841 ataccaagat gaggtctttg gtgggtttgt cactgagcct caggaggagt ctgaagaaga
     3901 agtagaggaa cctgaagaaa gacagcaaac acctgaggtg gtacctgatg attctggaac
     3961 tttctatgat caggcagttg tcagtaatga catggaagaa catttagagg agcctgttgc
     4021 tgaaccagag cctgatcctg aaccagaacc agaacaagaa cctgtatctg aaatccaaga
     4081 ggaaaagcct gagccagtat tagaagaaac tgcccctgag gatgctcaga agagttcttc
     4141 tccagcacct gcagacatag ctcagacagt acaggaagac ttgaggacat tttcttgggc
     4201 atctgtgacc agtaagaatc ttccacccag tggagctgtt ccagttactg ggataccacc
     4261 tcatgttgtt aaagtaccag cttcacagcc ccgtccagag tctaagcctg aatctcagat
     4321 tccaccacaa agacctcagc gggatcaaag agtgcgagaa caacgaataa atattcctcc
     4381 ccaaagggga cccagaccaa tccgtgaggc tggtgagcaa ggtgacattg aaccccgaag
     4441 aatggtgaga caccctgaca gtcaccaact cttcattggc aacctgcctc atgaagtgga
     4501 caaatcagag cttaaagatt tctttcaaag ttatggaaac gtggtggagt tgcgcattaa
     4561 cagtggtggg aaattaccca attttggttt tgttgtgttt gatgattctg agcctgttca
     4621 gaaagtcctt agcaacaggc ccatcatgtt cagaggtgag gtccgtctga atgtcgaaga
     4681 gaagaagact cgagctgcca gggaaggcga ccgacgagat aatcgccttc ggggacctgg
     4741 aggccctcga ggtgggctgg gtggtggaat gagaggccct ccccgtggag gcatggtgca
     4801 gaaaccagga tttggagtgg gaagggggct tgcgccacgg cagtgacctg caggcatgca
     4861 agcttgatat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
     4921 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     4981 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     5041 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     5101 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     5161 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     5221 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     5281 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     5341 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     5401 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     5461 ccgcctcccc gcatcgatac cgtcgacctc gagggaatta attcgagctc ggtaccttta
     5521 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     5581 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     5641 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     5701 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     5761 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagcatc
     5821 tagaattaat tccgtgtatt ctatagtgtc acctaaatcg tatgtgtatg atacataagg
     5881 ttatgtatta attgtagccg cgttctaacg acaatatgta caagcctaat tgtgtagcat
     5941 ctggcttact gaagcagacc ctatcatctc tctcgtaaac tgccgtcaga gtcggtttgg
     6001 ttggacgaac cttctgagtt tctggtaacg ccgtcccgca cccggaaatg gtcagcgaac
     6061 caatcagcag ggtcatcgct agccagatcc tctacgccgg acgcatcgtg gccggcatca
     6121 ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc
     6181 gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg
     6241 tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc
     6301 tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc
     6361 gtcgaatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     6421 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     6481 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     6541 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     6601 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     6661 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     6721 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     6781 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     6841 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     6901 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     6961 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     7021 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     7081 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     7141 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     7201 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     7261 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     7321 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     7381 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     7441 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     7501 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     7561 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     7621 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     7681 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     7741 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     7801 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     7861 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     7921 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     7981 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     8041 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     8101 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     8161 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     8221 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     8281 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     8341 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     8401 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     8461 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     8521 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     8581 gcgcgtcggc cgattcatta atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt
     8641 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     8701 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     8761 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     8821 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     8881 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     8941 gcaaaaagct tggacacaag acaggcttgc gagatatgtt tgagaatacc actttatccc
     9001 gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt
     9061 gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta
     9121 gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg
     9181 cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt
     9241 attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc
     9301 gtcatgtcga taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag
     9361 tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata
     9421 ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct
     9481 tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata
     9541 cctggattga acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct
     9601 aatcttttca acgcctggca ctgccgggcg ttgttctttt taacttcagg cgggttacaa
     9661 tagtttccag taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc
     9721 ctgttcaaac cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac
     9781 ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa ccatccctca ctggtatcgc
     9841 atgattaacc gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc
     9901 caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg atgatttata cgatacggtg
     9961 attggctacc gtggcggcaa ctggatttat gagtgggccc cggatctttg tgaaggaacc
    10021 ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt
    10081 aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt
    10141 ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga
    10201 aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca
    10261 acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga
    10321 attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat
    10381 ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt
    10441 aacctttata agtaggcata acagttataa tcataacata ctgttttttc ttactccaca
    10501 caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt
    10561 aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa
    10621 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
    10681 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
    10741 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
    10801 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
    10861 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca
    10921 attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa
    10981 gaaattgtat ttgttaaata tgtactacaa acttagtagt tggaagggct aattcactcc
    11041 caaagaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
    11101 tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac
    11161 aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc
    11221 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg
    11281 aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc
    11341 aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg
    11401 cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct
    11461 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
    11521 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
    11581 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt ag
//
