LOCUS       sequence-474742-       11823 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses NES-dCas13b-ABI for CRISPR-TO perturbation in primary 
            mouse cortical neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11823)
  AUTHORS   Han M, Fu ML, Zhu Y, Choi AA, Li E, Bezney J, Cai S, Miles L, Ma Y, 
            Qi LS
  TITLE     Programmable control of spatial transcriptome in live cells and 
            neurons.
  JOURNAL   Nature. 2025 Jul;643(8070):241-251. doi: 10.1038/s41586-025-09020-z.
            Epub 2025 May 21.
  PUBMED    40399675
REFERENCE   2  (bases 1 to 11823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-025-09020-z"; journalName: "Nature"; date: 
            "2025-07"; volume: "643"; issue: "8070"; pages: "241-251"
COMMENT     Sequence Label: pSLQ10625
FEATURES             Location/Qualifiers
     source          1..11823
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(26..614)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(106..125)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(785..1576,1577..1645))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1408..1427
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1646..1750)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        1781..2160
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2162..2439
                     /label=chicken beta-actin promoter
     intron          2440..3456
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3380..3402
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3464..3483
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3527..3550
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(6920..6922,6923..7633)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(6920..6922,6923..7633)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6962..6983)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7223..7242)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7570..7591
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             8579..8620
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(8721..8740)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8786..8841
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8840..8859)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9202..9218)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9202..9218)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9215..9237)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9226..9242
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9250..9256,9257..9274,9275..9280))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9295..9316
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9375..9570
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9421..9556
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        9640..10139
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9675..9698)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     10072..10091
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             10158..11183
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             10158..11183
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(11513..11532)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11567..11586
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11678..11695)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
       61 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      121 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      181 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      241 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      301 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      361 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      421 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
      481 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      541 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      601 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      661 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
      721 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
      781 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
      841 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
      901 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
      961 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1021 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1081 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1141 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1201 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     1261 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     1321 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     1381 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     1441 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     1501 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     1561 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     1621 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     1681 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     1741 gggttccgcg cacatttccc cgaaaagtgc cacctgggtc gacattgatt attgactagt
     1801 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     1861 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     1921 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     1981 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     2041 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     2101 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     2161 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
     2221 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg
     2281 gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt
     2341 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
     2401 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt
     2461 cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg
     2521 ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg
     2581 gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag
     2641 ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc
     2701 gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt
     2761 tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg
     2821 gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg
     2881 ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag
     2941 cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg
     3001 ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag
     3061 ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc
     3121 cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa
     3181 atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg
     3241 aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc
     3301 cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg
     3361 gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct
     3421 gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt
     3481 gtgctgtctc atcattttgg caaagaattc ctcgagcggc cgcatggatt acaaggacga
     3541 cgatgacaag ggatccggcg gaccgctgta ccctgagcgg ctgcggcgga tcctgaccgg
     3601 atccctcgag aacatccccg ctctggtgga aaaccagaag aagtactttg gcacctacag
     3661 cgtgatggcc atgctgaacg ctcagaccgt gctggaccac atccagaagg tggccgatat
     3721 tgagggcgag cagaacgaga acaacgagaa tctgtggttt caccccgtga tgagccacct
     3781 gtacaacgcc aagaacggct acgacaagca gcccgagaaa accatgttca tcatcgagcg
     3841 gctgcagagc tacttcccat tcctgaagat catggccgag aaccagagag agtacagcaa
     3901 cggcaagtac aagcagaacc gcgtggaagt gaacagcaac gacatcttcg aggtgctgaa
     3961 gcgcgccttc ggcgtgctga agatgtacag ggacctgacc aacgcataca agacctacga
     4021 ggaaaagctg aacgacggct gcgagttcct gaccagcaca gagcaacctc tgagcggcat
     4081 gatcaacaac tactacacag tggccctgcg gaacatgaac gagagatacg gctacaagac
     4141 agaggacctg gccttcatcc aggacaagcg gttcaagttc gtgaaggacg cctacggcaa
     4201 gaaaaagtcc caagtgaata ccggattctt cctgagcctg caggactaca acggcgacac
     4261 acagaagaag ctgcacctga gcggagtggg aatcgccctg ctgatctgcc tgttcctgga
     4321 caagcagtac atcaacatct ttctgagcag gctgcccatc ttctccagct acaatgccca
     4381 gagcgaggaa cggcggatca tcatcagatc cttcggcatc aacagcatca agctgcccaa
     4441 ggaccggatc cacagcgaga agtccaacaa gagcgtggcc atggatatgc tcaacgaagt
     4501 gaagcggtgc cccgacgagc tgttcacaac actgtctgcc gagaagcagt cccggttcag
     4561 aatcatcagc gacgaccaca atgaagtgct gatgaagcgg agcagcgaca gattcgtgcc
     4621 tctgctgctg cagtatatcg attacggcaa gctgttcgac cacatcaggt tccacgtgaa
     4681 catgggcaag ctgagatacc tgctgaaggc cgacaagacc tgcatcgacg gccagaccag
     4741 agtcagagtg atcgagcagc ccctgaacgg cttcggcaga ctggaagagg ccgagacaat
     4801 gcggaagcaa gagaacggca ccttcggcaa cagcggcatc cggatcagag acttcgagaa
     4861 catgaagcgg gacgacgcca atcctgccaa ctatccctac atcgtggaca cctacacaca
     4921 ctacatcctg gaaaacaaca aggtcgagat gtttatcaac gacaaagagg acagcgcccc
     4981 actgctgccc gtgatcgagg atgatagata cgtggtcaag acaatcccca gctgccggat
     5041 gagcaccctg gaaattccag ccatggcctt ccacatgttt ctgttcggca gcaagaaaac
     5101 cgagaagctg atcgtggacg tgcacaaccg gtacaagaga ctgttccagg ccatgcagaa
     5161 agaagaagtg accgccgaga atatcgccag cttcggaatc gccgagagcg acctgcctca
     5221 gaagatcctg gatctgatca gcggcaatgc ccacggcaag gatgtggacg ccttcatcag
     5281 actgaccgtg gacgacatgc tgaccgacac cgagcggaga atcaagagat tcaaggacga
     5341 ccggaagtcc attcggagcg ccgacaacaa gatgggaaag agaggcttca agcagatctc
     5401 cacaggcaag ctggccgact tcctggccaa ggacatcgtg ctgtttcagc ccagcgtgaa
     5461 cgatggcgag aacaagatca ccggcctgaa ctaccggatc atgcagagcg ccattgccgt
     5521 gtacgatagc ggcgacgatt acgaggccaa gcagcagttc aagctgatgt tcgagaaggc
     5581 ccggctgatc ggcaagggca caacagagcc tcatccattt ctgtacaagg tgttcgcccg
     5641 cagcatcccc gccaatgccg tcgagttcta cgagcgctac ctgatcgagc ggaagttcta
     5701 cctgaccggc ctgtccaacg agatcaagaa aggcaacaga gtggatgtgc ccttcatccg
     5761 gcgggaccag aacaagtgga aaacacccgc catgaagacc ctgggcagaa tctacagcga
     5821 ggatctgccc gtggaactgc ccagacagat gttcgacaat gagatcaagt cccacctgaa
     5881 gtccctgcca cagatggaag gcatcgactt caacaatgcc aacgtgacct atctgatcgc
     5941 cgagtacatg aagagagtgc tggacgacga cttccagacc ttctaccagt ggaaccgcaa
     6001 ctaccggtac atggacatgc ttaagggcga gtacgacaga aagggctccc tgcagcactg
     6061 cttcaccagc gtggaagaga gagaaggcct ctggaaagag cgggcctcca gaacagagcg
     6121 gtacagaaag caggccagca acaagatccg cagcaaccgg cagatgagaa acgccagcag
     6181 cgaagagatc gagacaatcc tggataagcg gctgagcaac agccggaacg agtaccagaa
     6241 aagcgagaaa gtgatccggc gctacagagt gcaggatgcc ctgctgtttc tgctggccaa
     6301 aaagaccctg accgaactgg ccgatttcga cggcgagagg ttcaaactga aagaaatcat
     6361 gcccgacgcc gagaagggaa tcctgagcga gatcatgccc atgagcttca ccttcgagaa
     6421 aggcggcaag aagtacacca tcaccagcga gggcatgaag ctgaagaact acggcgactt
     6481 ctttgtgctg gctagcgaca agaggatcgg caacctgctg gaactcgtgg gcagcgacat
     6541 cgtgtccaaa gaggatatca tggaagagtt caacaaatac gaccagtgca ggcccgagat
     6601 cagctccatc gtgttcaacc tggaaaagtg ggccttcgac acataccccg agctgtctgc
     6661 cagagtggac cgggaagaga aggtggactt caagagcatc ctgaaaatcc tgctgaacaa
     6721 caagaacatc aacaaagagc agagcgacat cctgcggaag atccggaacg ccttcgatgc
     6781 aaacaattac cccgacaaag gcgtggtgga aatcaaggcc ctgcctgaga tcgccatgag
     6841 catcaagaag gcctttgggg agtacgccat catgaaggcg gccgcggagg gagctctaga
     6901 ggcgctaccg gtcgccaccg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     6961 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     7021 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     7081 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     7141 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     7201 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     7261 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     7321 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     7381 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     7441 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     7501 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     7561 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     7621 cgagctgtac aagcatatgg gatccggatc cacgcgtgtg cctttgtatg gttttacttc
     7681 gatttgtgga agaagacctg agatggaagc tgctgtttcg actataccaa gattccttca
     7741 atcttcctct ggttcgatgt tagatggtcg gtttgatcct caatccgccg ctcatttctt
     7801 cggtgtttac gacggccatg gcggttctca ggtagcgaac tattgtagag agaggatgca
     7861 tttggctttg gcggaggaga tagctaagga gaaaccgatg ctctgcgatg gtgatacgtg
     7921 gctggagaag tggaagaaag ctcttttcaa ctcgttcctg agagttgact cggagattga
     7981 gtcagttgcg ccggagacgg ttgggtcaac gtcggtggtt gccgttgttt tcccgtctca
     8041 catcttcgtc gctaactgcg gtgactctag agccgttctt tgccgcggca aaactgcact
     8101 tccattatcc gttgaccata aaccggatag agaagatgaa gctgcgagga ttgaagccgc
     8161 aggagggaaa gtgattcagt ggaatggagc tcgtgttttc ggtgttctcg ccatgtcgag
     8221 atccattggc gatagatact tgaaaccatc catcattcct gatccggaag tgacggctgt
     8281 gaagagagta aaagaagatg attgtctgat tttggcgagt gacggggttt gggatgtaat
     8341 gacggatgaa gaagcgtgtg agatggcaag gaagcggatt ctcttgtggc acaagaaaaa
     8401 cgcggtggct ggggatgcat cgttgctcgc ggatgagcgg agaaaggaag ggaaagatcc
     8461 tgcggcgatg tccgcggctg agtatttgtc aaagctggcg atacagagag gaagcaaaga
     8521 caacataagt gtggtggtgg ttgatttgaa gtcaagcgct gcaggtgcta ccactagtgg
     8581 caagcccatc cccaaccccc tgctgggcct ggacagcacc taaggtaccg gttcgaggaa
     8641 ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caatgccctg
     8701 gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag
     8761 ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt
     8821 tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat
     8881 cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac
     8941 aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta
     9001 ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt
     9061 ttttctttaa catccctaaa attttcctta catgttttac tagccagatt tttcctcctc
     9121 tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct cgacctgcag
     9181 cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     9241 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     9301 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     9361 gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     9421 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     9481 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     9541 ggcttttttg gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttaagctt
     9601 tcgcgagctc gagatctgat atcatcgatg aattctaccg ggtaggggag gcgcttttcc
     9661 caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg
     9721 gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg
     9781 gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc
     9841 agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga
     9901 tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc
     9961 tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca
    10021 ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca
    10081 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga
    10141 ccgatccagc cgccaccatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc
    10201 tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc
    10261 gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg
    10321 atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc
    10381 cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg
    10441 cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg
    10501 tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc
    10561 cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg
    10621 ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg
    10681 cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg
    10741 tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca
    10801 ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct
    10861 ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg
    10921 agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct
    10981 atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg
    11041 caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg
    11101 ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca
    11161 ctcgtccgag ggcaaaggaa tagtcgagaa attgatgatc tattaaacaa taaagatgtc
    11221 cactaaaatg gaagtttttc ctgtcatact ttgttaagaa gggtgagaac agagtaccta
    11281 cattttgaat ggaaggattg gagctacggg ggtgggggtg gggtgggatt agataaatgc
    11341 ctgctcttta ctgaaggctc tttactattg ctttatgata atgtttcata gttggatatc
    11401 ataatttaaa caagcaaaac caaattaagg gccagctcat tcctcccact catgatctat
    11461 agatcctcta gagtcgacct gcaggcatgc aagcttaatg gttacaaata aagcaatagc
    11521 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    11581 ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc caacgcgcgg
    11641 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
    11701 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
    11761 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
    11821 acc
//
