LOCUS       sequence-474745-       10719 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses LRRK1 K746G in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10719)
  AUTHORS   Wang X, Xu P, Bentley-DeSousa A, Hancock-Cerutti W, Cai S, Johnson 
            BT, Tonelli F, Shao L, Talaia G, Alessi DR, Ferguson SM, De Camilli 
            P
  TITLE     The bridge-like lipid transport protein VPS13C/PARK23 mediates 
            ER-lysosome contacts following lysosome damage.
  JOURNAL   Nat Cell Biol. 2025 May;27(5):776-789. doi: 
            10.1038/s41556-025-01653-6. Epub 2025 Apr 10.
  PUBMED    40211074
REFERENCE   2  (bases 1 to 10719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-025-01653-6"; journalName: "Nat Cell Biol"; date: 
            "2025-05"; volume: "27"; issue: "5"; pages: "776-789"
COMMENT     Sequence Label: mScarlet-LRRK1 K746G
FEATURES             Location/Qualifiers
     source          1..10719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             613..1308
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(746..764)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1014..1033
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3361..3372
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     polyA_signal    7507..7628
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7544..7563)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7598..7617
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7635..8090)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7772..7793)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7984..8003
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8117..8221
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8223..8580
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8223..8414
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8246..8266)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8431..8566
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8615..9409
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8615..9409
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8669..8688)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9279..9298
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9597..9616)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9641..9688
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10017..10605
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10506..10525
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gcagtgatca aggagttcat gcggttcaag
      661 gtgcacatgg agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc
      721 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
      781 ttctcctggg acatcctgtc ccctcagttc atgtacggct ccagggcctt catcaagcac
      841 cccgccgaca tccccgacta ctataagcag tccttccccg agggcttcaa gtgggagcgc
      901 gtgatgaact tcgaggacgg cggcgccgtg accgtgaccc aggacacctc cctggaggac
      961 ggcaccctga tctacaaggt gaagctccgc ggcaccaact tccctcctga cggccccgta
     1021 atgcagaaga agacaatggg ctgggaagcg tccaccgagc ggttgtaccc cgaggacggc
     1081 gtgctgaagg gcgacattaa gatggccctg cgcctgaagg acggcggccg ctacctggcg
     1141 gacttcaaga ccacctacaa ggccaagaag cccgtgcaga tgcccggcgc ctacaacgtc
     1201 gaccgcaagt tggacatcac ctcccacaac gaggactaca ccgtggtgga acagtacgaa
     1261 cgctccgagg gccgccactc caccggcggc atggacgagc tgtacaagtc cggactcaga
     1321 tctcgagcta tggctggcat gtcgcaaaga ccccccagca tgtactggtg tgtggggccg
     1381 gaggagtcag ctgtgtgtcc agaacgtgcc atggagacgc ttaacggtgc cggggacacg
     1441 ggcggcaagc cgtccacgcg gggcggtgac cctgcagcgc ggtcccgcag gacggaaggc
     1501 atccgcgccg cgtacaggcg gggagaccgc ggcggcgccc gggacctgct ggaggaggcc
     1561 tgcgaccagt gcgcgtccca gctggaaaag ggccagcttc tgagcatccc ggcagcctat
     1621 ggggatctgg agatggtccg ctacctactc agcaagagac tggtggagct gcccaccgag
     1681 cccacggatg acaacccagc cgtggtggca gcgtattttg gacacacggc agttgtgcag
     1741 gaattgcttg agtccttacc aggtccctgc agtccccagc ggcttctgaa ctggatgctg
     1801 gccttggctt gccagcgagg gcacctgggg gttgtgaagc tcctggtcct gacgcacggg
     1861 gctgacccgg agagctacgc tgtcaggaag aatgagttcc ctgtcatcgt gcgcttgccc
     1921 ctgtatgcgg ccatcaagtc agggaatgaa gacattgcaa tattcctgct tcggcatggg
     1981 gcctatttct gttcctacat cttgctggat agtcctgacc ccagcaaaca tctgctgaga
     2041 aagtacttca ttgaagccag tcccttgccc agcagttatc cgggaaaaac agctctccgt
     2101 gtgaaatggt cccatctcag actgccctgg gtagacctag actggctcat agacatctcc
     2161 tgccagatca cggagctcga cctttctgcc aactgcctgg cgaccctccc ctcggttatc
     2221 ccctggggcc tcatcaatct ccggaagctg aacctctccg acaaccacct gggggagctg
     2281 cctggcgtgc agtcatcgga cgaaatcatc tgttccaggc tacttgaaat tgacatttcc
     2341 agcaacaagt tgtcccacct ccctcctgga ttcttgcacc tctcaaaact tcaaaaactg
     2401 acagcttcaa aaaattgttt agaaaaattg ttcgaagaag aaaatgccac taactggata
     2461 ggtttacgga agctacagga acttgatata tctgacaata aattgacaga actccctgcc
     2521 ctgttccttc actctttcaa gtccctcaat tctctgaatg tctccagaaa caacctgaag
     2581 gtgtttccag atccctgggc ctgccctttg aaatgttgta aagcttccag aaatgccctg
     2641 gaatgtctgc cagacaaaat ggctgtcttt tggaaaaatc acctgaagga tgtggatttc
     2701 tcagaaaacg cactcaaaga agttcccctg ggacttttcc agcttgatgc cctcatgttc
     2761 ttgaggttac aggggaacca gctggcggca cttccacctc aagagaagtg gacctgcagg
     2821 cagctcaaaa ccctggatct ctccagaaac caacttggca aaaatgaaga tggactgaaa
     2881 acgaagcgta ttgccttttt caccaccaga ggtcgccagc gctccgggac tgaggcagca
     2941 agtgtgctgg aatttccggc cttcctaagt gagtctttgg aagtcctttg cctgaacgac
     3001 aaccacctcg acacagtccc tccctcggtt tgcctactga agagcttatc agagctctac
     3061 ttgggaaaca accctggcct ccgggagctc cctcctgagc tggggcagct gggcaacctc
     3121 tggcagctgg acactgaaga cctgaccatc agcaatgtgc ctgcagaaat ccaaaaagaa
     3181 ggccccaaag caatgctgtc ttacctgcgt gctcagctgc ggaaagcgga aaagtgcaag
     3241 ctgatgaaga tgatcatcgt gggtcccccg cgccagggca agtccaccct cctggagatc
     3301 ttacagacgg ggagggcccc ccaggtggtg catggagagg ccaccatcag gaccaccaag
     3361 tgggagctcc agaggccggc tggctcaaga gccaaggttg agtccgtgga gttcaacgtc
     3421 tgggacatcg ggggaccggc cagcatggcc actgtcaacc agtgcttctt cacggacaag
     3481 gccctgtacg tggtggtctg gaacctggcg ctgggggagg aggccgtggc caacctccag
     3541 ttctggctgc tcaacatcga ggccggggcc ccaaacgccg tggtgctggt ggtcgggacg
     3601 cacctggatt taattgaagc caagttccgt gtggaaagga ttgcaacgct gcgtgcctat
     3661 gtgctggcac tctgccgctc cccctccggc tccagggcca caggcttccc agacatcacc
     3721 ttcaaacact tacatgagat ttcctgcaag agcctggaag gtcaggaagg gctgcgacag
     3781 ctgattttcc acgtcacgtg cagcatgaag gacgtgggca gcaccatcgg ctgccagcga
     3841 ctggcagggc ggctgatccc caggagctac ctgagcctgc aggaggccgt gctggcagag
     3901 cagcagcgcc gcagccggga cgacgacgtg cagtacctga cggacaggca gctggagcag
     3961 ctggtggagc agacgcccga caacgacatc aaggactacg aggacctgca gtcagccatc
     4021 agcttcctca tagaaaccgg caccctgctc catttcccgg acaccagcca cggcctgagg
     4081 aacctctact tcctcgaccc tatttggctc tccgaatgtc tgcagaggat ctttaatatt
     4141 aagggctctc ggtcagtggc caagaatggg gtgatcagag cagaagacct caggatgctg
     4201 ctggtgggga ctggcttcac gcagcagacg gaagagcagt acttccagtt cctggccaag
     4261 tttgagatcg ccctgcccgt cgccaatgac agctacctcc tgccccatct ccttccatct
     4321 aaacctggcc tggacaccca cggtatgcgg caccccacag ccaacaccat tcagagggta
     4381 tttaagatga gcttcgttcc cgttggcttc tggcaaaggt ttatagcacg gatgctgatc
     4441 agcctggcgg agatggacct gcagcttttt gaaaacaaga agaatactaa aagcaggaac
     4501 aggaaagtca ccatttacag ttttacagga aaccagagaa atcgctgtag cacattcaga
     4561 gtgaaaagaa atcagaccat ctattggcag gaagggctcc tggtcacttt tgatgggggc
     4621 tacctcagtg tggaatcttc cgacgtgaac tggaaaaaga agaaaagcgg aggaatgaaa
     4681 attgtttgcc aatcagaagt gagggacttc tcagccatgg ctttcatcac ggaccacgtc
     4741 aattccttga ttgatcagtg gtttcccgcc ctgacagcca cagagagcga cgggacgcca
     4801 ctcatggagc agtacgtgcc ctgcccggtc tgcgagacag cctgggccca gcacacggac
     4861 cccagtgaga aatcagagga tgtgcagtac ttcgacatgg aagactgtgt cctgacggcc
     4921 atcgagcggg acttcatctc ctgccccaga cacccggacc tccccgtgcc gctgcaggag
     4981 ctggtccctg aactgttcat gaccgacttc ccggccaggc tcttcctgga gaacagcaag
     5041 ctggagcaca gcgaggacga gggcagcgtc ctgggccagg gcggcagtgg caccgtcatc
     5101 taccgggccc ggtaccaggg ccagcctgtg gccgtcaagc gcttccacat caaaaaattc
     5161 aagaactttg ctaacgtacc ggcagacacc atgctgaggc acctgcgggc caccgatgcc
     5221 atgaagaact tctccgagtt ccggcaggag gccagcatgc tgcacgcgct gcagcacccc
     5281 tgcatcgtgg cgctcatcgg catcagcatc cacccgctct gcttcgccct ggagctcgcg
     5341 ccgctcagca gcctcaacac cgtgctgtcc gagaacgcca gagattcttc ctttataccc
     5401 ctgggacaca tgctcaccca aaaaatagcc taccagatcg cctcgggcct ggcctacctg
     5461 cacaagaaaa acatcatctt ctgtgacctg aagtcggaca acattctggt gtggtccctt
     5521 gacgtcaagg agcacatcaa catcaagcta tctgactacg ggatttcgag gcagtcattc
     5581 catgagggcg ccctaggcgt ggagggcact cctggctacc aggccccaga gatcaggcct
     5641 cgcattgtat atgatgagaa ggtagatatg ttctcctatg gaatggtgct ctacgagttg
     5701 ctgtcaggac agcgccctgc actgggccac caccagctcc agattgccaa gaagctgtcc
     5761 aagggcatcc gcccggttct ggggcagccg gaggaagtgc agttccggcg actgcaggcg
     5821 ctcatgatgg agtgctggga cactaagcca gagaagcgac cgctggccct gtcggtggtg
     5881 agccagatga aggacccgac ttttgccacc ttcatgtatg aactgtgctg tgggaagcag
     5941 acagccttct tctcatccca gggccaggag tacaccgtgg tgttttggga tggaaaagag
     6001 gagtccagga actacacggt ggtgaacaca gagaagggcc tcatggaggt gcagaggatg
     6061 tgctgccctg ggatgaaggt gagctgccag ctccaggtcc agagatccct gtggacagcc
     6121 accgaggacc agaaaatcta catctacacc ctcaagggca tgtgcccctt aaacacaccc
     6181 caacaggcct tggatactcc agctgtcgtc acctgcttct tggccgtgcc tgttattaaa
     6241 aagaattcct acctggtctt agcgggcctc gccgatgggc ttgtggctgt gtttcccgtg
     6301 gtgcggggca ccccaaagga cagctgctcc tacctgtgct cacacacagc caacaggtcc
     6361 aagttcagca tcgcggatga agacgcacgg cagaacccct acccagtgaa ggccatggag
     6421 gtggtcaaca gcggctctga ggtctggtac agcaatgggc cgggcctcct tgtcatcgac
     6481 tgtgcctccc tggagatctg caggcggctg gagccctaca tggccccctc catggttacg
     6541 tcagtcgtgt gcagctctga gggcagaggg gaggaggtcg tctggtgcct ggatgacaag
     6601 gccaactcct tggtgatgta ccactccacc acctaccagc tgtgtgcccg gtacttctgc
     6661 ggggtcccca gccccctcag ggacatgttt cccgtgcggc ccttggacac ggaacccccg
     6721 gcagccagcc acacggccaa cccaaaggtg cctgaggggg actccatcgc ggacgtgagc
     6781 atcatgtaca gtgaggagct gggcacgcag atcctgatcc accaggaatc actcactgac
     6841 tactgctcca tgtcctccta ctcctcatcc ccaccccgcc aggctgccag gtccccctca
     6901 agcctcccca gctccccagc aagttcttcc agtgtgcctt tctccaccga ctgcgaggac
     6961 tcagacatgc tacatacgcc cggtgctgcc tccgacaggt ctgagcatga cctgaccccc
     7021 atggacgggg agaccttcag ccagcacctg caggccgtga agatcctcgc cgtcagagac
     7081 ctcatttggg tccccaggcg cggtggagat gttatcgtca ttggcctgga gaaggattct
     7141 ggcgcccagc ggggccgagt cattgccgtc ttaaaagccc gagagctgac tccgcatggg
     7201 gtgctggtgg atgctgccgt ggtggcaaag gacactgttg tgtgcacctt tgaaaatgaa
     7261 aacacagagt ggtgcctggc cgtctggagg ggctggggcg ccagggagtt cgacattttc
     7321 taccagtcct acgaggagct gggccggctg gaggcttgca ctcgcaagag aaggtaagga
     7381 tccaccggat ctagataact gatcataatc agccatacca catttgtaga ggttttactt
     7441 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     7501 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     7561 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     7621 gtatcttaac gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     7681 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     7741 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     7801 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     7861 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     7921 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     7981 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     8041 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac
     8101 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     8161 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     8221 tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     8281 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     8341 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     8401 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     8461 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     8521 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     8581 gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     8641 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     8701 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     8761 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg
     8821 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     8881 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     8941 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     9001 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     9061 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     9121 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg
     9181 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     9241 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     9301 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     9361 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     9421 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     9481 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     9541 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa
     9601 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
     9661 aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac
     9721 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc
     9781 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca
     9841 ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt
     9901 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     9961 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
    10021 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
    10081 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
    10141 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
    10201 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
    10261 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
    10321 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
    10381 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
    10441 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
    10501 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
    10561 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
    10621 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
    10681 tcccctgatt ctgtggataa ccgtattacc gccatgcat
//
