LOCUS       sequence-474746-       10327 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses PYL1-MAVS* for recruiting RNA to the outer mitochondrial 
            membrane via CRISPR-TO.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10327)
  AUTHORS   Han M, Fu ML, Zhu Y, Choi AA, Li E, Bezney J, Cai S, Miles L, Ma Y, 
            Qi LS
  TITLE     Programmable control of spatial transcriptome in live cells and 
            neurons.
  JOURNAL   Nature. 2025 Jul;643(8070):241-251. doi: 10.1038/s41586-025-09020-z.
            Epub 2025 May 21.
  PUBMED    40399675
REFERENCE   2  (bases 1 to 10327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-025-09020-z"; journalName: "Nature"; date: 
            "2025-07"; volume: "643"; issue: "8070"; pages: "241-251"
COMMENT     Sequence Label: pSLQ10550
FEATURES             Location/Qualifiers
     source          1..10327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             42..675
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    722..847
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1344..1577
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1762..1806
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2091..2208
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2273..2772
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2308..2331)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2705..2724
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3388..4083
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     misc_feature    4232..4820
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4285..4305)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4703..4714)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4823..4839)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4824..4840)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4930..5163
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5188..5206)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5189..5206)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5496..5515)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5729..5748)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5848..5870
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5908..5926)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5994..6098
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6099..6167,6168..6959)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6317..6336)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7130..7718
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7619..7638
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7872..7889
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7959..7979)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7964..8293
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8144..8279
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9427..9492
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9622..9642
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10067..10201
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10104..10123)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10158..10177
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc
       61 ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
      121 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
      181 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
      241 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
      301 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
      361 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
      421 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
      481 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
      541 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
      601 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
      661 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
      721 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
      781 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
      841 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
      901 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
      961 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     1021 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     1081 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     1141 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc
     1201 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     1261 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     1321 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     1381 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     1441 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     1501 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     1561 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     1621 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     1681 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     1741 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     1801 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     1861 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     1921 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     1981 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
     2041 ttagtgaacg gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga
     2101 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2161 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
     2221 agggacagca gagatccagt ttggatcgat aagcttgata tcgaattcta ccgggtaggg
     2281 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg
     2341 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg
     2401 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
     2461 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact
     2521 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc
     2581 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc
     2641 gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc
     2701 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct
     2761 ccgggccttt cgacctgcag cccaagctta cgcgtctgca ggatatcaag cttgcggtac
     2821 gactgagtca tgggtggggg cgcgccaact caagacgaat tcacccaact ctcccaatca
     2881 atcgccgagt tccacacgta ccaactcggt aacggccgtt gctcatctct cctagctcag
     2941 cgaatccacg cgccgccgga aacagtatgg tccgtggtga gacgtttcga taggccacag
     3001 atttacaaac acttcatcaa aagctgtaac gtgagtgaag atttcgagat gcgagtggga
     3061 tgcacgcgcg acgtgaacgt gataagtgga ttaccggcga atacgtctcg agagagatta
     3121 gatctgttgg acgatgatcg gagagtgact gggtttagta taaccggtgg tgaacatagg
     3181 ctgaggaatt ataaatcggt tacgacggtt catagatttg agaaagaaga agaagaagaa
     3241 aggatctgga ccgttgtttt ggaatcttat gttgttgatg taccggaagg taattcggag
     3301 gaagatacga gattgtttgc tgatacggtt attagattga atcttcagaa acttgcttcg
     3361 atcactgaag ctatgaacgt ggatccgagc gagctgatta aggagaacat gcacatgaag
     3421 ctgtacatgg agggcaccgt ggacaaccat cacttcaagt gcacatccga gggcgaaggc
     3481 aagccctacg agggcaccca gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc
     3541 ttcgccttcg acatcctggc tactagcttc ctctacggca gcaagacctt catcaaccac
     3601 acccagggca tccccgactt cttcaagcag tccttccctg agggcttcac atgggagaga
     3661 gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac
     3721 ggctgcctca tctacaacgt caagatcaga ggggtgaact tcacatccaa cggccctgtg
     3781 atgcagaaga aaacactcgg ctgggaggcc ttcaccgaga cgctgtaccc cgctgacggc
     3841 ggcctggaag gcagaaacga catggccctg aagctcgtgg gcgggagcca tctgatcgca
     3901 aacatcaaga ccacatatag atccaagaaa cccgctaaga acctcaagat gcctggcgtc
     3961 tactatgtgg actacagact ggaaagaatc aaggaggcca acaacgagac ctacgtcgag
     4021 cagcacgagg tggcagtggc cagatactgc gacctcccta gcaaactggg gcacaagctt
     4081 aatcggaccg caagcaagag gccctcacct ggggctctgt ggctccaggt ggctgtgaca
     4141 ggggtgctgg tagtcacact cctggtggtg ctgtaccggc ggcgtctgca ctagcctgca
     4201 ggcatgcaag cttgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
     4261 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     4321 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     4381 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     4441 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     4501 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     4561 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     4621 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     4681 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     4741 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     4801 cctttgggcc gcctccccgc atcgataccg tcgacctcga gggaattaat tcgagctcgg
     4861 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     4921 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     4981 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     5041 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     5101 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     5161 gcagcatcta gaattaattc cgtgtattct atagtgtcac ctaaatcgta tgtgtatgat
     5221 acataaggtt atgtattaat tgtagccgcg ttctaacgac aatatgtaca agcctaattg
     5281 tgtagcatct ggcttactga agcagaccct atcatctctc tcgtaaactg ccgtcagagt
     5341 cggtttggtt ggacgaacct tctgagtttc tggtaacgcc gtcccgcacc cggaaatggt
     5401 cagcgaacca atcagcaggg tcatcgctag ccagatcctc tacgccggac gcatcgtggc
     5461 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
     5521 ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc
     5581 aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc
     5641 ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa
     5701 gggagagcgt cgaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     5761 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     5821 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     5881 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     5941 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     6001 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     6061 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     6121 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     6181 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     6241 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     6301 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     6361 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     6421 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     6481 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     6541 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     6601 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     6661 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     6721 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6781 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6841 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6901 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6961 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     7021 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     7081 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     7141 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     7201 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     7261 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     7321 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     7381 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     7441 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7501 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7561 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7621 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7681 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7741 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     7801 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     7861 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     7921 ctctccccgc gcgtcggccg attcattaat gcagctgtgg aatgtgtgtc agttagggtg
     7981 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     8041 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     8101 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     8161 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     8221 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     8281 aggcttttgc aaaaagcttg gacacaagac aggcttgcga gatatgtttg agaataccac
     8341 tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg
     8401 tttttagtgc gccagatctc tataatctcg cgcaacctat tttcccctcg aacacttttt
     8461 aagccgtaga taaacaggct gggacacttc acatgagcga aaaatacatc gtcacctggg
     8521 acatgttgca gatccatgca cgtaaactcg caagccgact gatgccttct gaacaatgga
     8581 aaggcattat tgccgtaagc cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact
     8641 gggtattcgt catgtcgata ccgtttgtat ttccagctac gatcacgaca accagcgcga
     8701 gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct
     8761 ggtggatacc ggtggtactg cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt
     8821 caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat gactatgttg ttgatatccc
     8881 gcaagatacc tggattgaac agccgtggga tatgggcgtc gtattcgtcc cgccaatctc
     8941 cggtcgctaa tcttttcaac gcctggcact gccgggcgtt gttcttttta acttcaggcg
     9001 ggttacaata gtttccagta agtattctgg aggctgcatc catgacacag gcaaacctga
     9061 gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta gtccgccgct
     9121 ttaatcacgg cgcacaaccg cctgtgcagt cggcccttga tggtaaaacc atccctcact
     9181 ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc
     9241 tcgacgtcca ggcacgtatt gtgatgagcg atgccgaacg taccgacgat gatttatacg
     9301 atacggtgat tggctaccgt ggcggcaact ggatttatga gtgggccccg gatctttgtg
     9361 aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc
     9421 tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt
     9481 tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt
     9541 aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct
     9601 gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt
     9661 ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc
     9721 tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa
     9781 tattctgtaa cctttataag taggcataac agttataatc ataacatact gttttttctt
     9841 actccacaca ggcatagagt gtctgctatt aataactatg ctcaaaaatt gtgtaccttt
     9901 agctttttaa tttgtaaagg ggttaataag gaatatttga tgtatagtgc cttgactaga
     9961 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
    10021 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
    10081 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    10141 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
    10201 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac
    10261 cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc
    10321 attttaa
//
