LOCUS       sequence-474747-       14684 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses NES-dCas13b-ABI for CRISPR-TO perturbation in the 
            cytoplasm of cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14684)
  AUTHORS   Han M, Fu ML, Zhu Y, Choi AA, Li E, Bezney J, Cai S, Miles L, Ma Y, 
            Qi LS
  TITLE     Programmable control of spatial transcriptome in live cells and 
            neurons.
  JOURNAL   Nature. 2025 Jul;643(8070):241-251. doi: 10.1038/s41586-025-09020-z.
            Epub 2025 May 21.
  PUBMED    40399675
REFERENCE   2  (bases 1 to 14684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-025-09020-z"; journalName: "Nature"; date: 
            "2025-07"; volume: "643"; issue: "8070"; pages: "241-251"
COMMENT     Sequence Label: pSLQ10530
FEATURES             Location/Qualifiers
     source          1..14684
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(186..230)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    415..648
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1145..1270
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1317..1950
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    2118..2252
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2142..2161)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2196..2215
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2677..2697)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          2827..2892
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(4026..4355)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4040..4175
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4340..4360
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(4430..4447)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4601..5189)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4681..4700)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5360..6151,6152..6220))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5983..6002
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6221..6325)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6393..6411
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6449..6471)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6571..6590
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6804..6823
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        7113..7131
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     7113..7130
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             7156..7389
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     7479..7495
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     7480..7496
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    7499..8087
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             7605..7616
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     8014..8034
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8128..8169)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(join(9115..9825,9826..9828))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             complement(join(9115..9825,9826..9828))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(9157..9178)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     9506..9525
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9765..9786
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(13198..13221)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      13221..13230
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          13245..14183
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(13268..13288)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        complement(14184..14395)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    14468..14585
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tcgggcctgt cgggtcccct cggggttggg aggtgggtct gaaacgataa tggtgaatat
       61 ccctgcctaa ctctattcac tatagaaagt acagcaaaaa ctattcttaa acctaccaag
      121 cctcctacta tcattatgaa taattttata taccacagcc aatttgttat gttaaaccaa
      181 ttccacaaac ttgcccattt atctaattcc aataattctt gttcattctt ttcttgctgg
      241 ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt aattgttaat ttctctgtcc
      301 cactccatcc aggtcgtgtg attccaaatc tgttccagag atttattact ccaactagca
      361 ttccaaggca cagcagtggt gcaaatgagt tttccagagc aaccccaaat ccccaggagc
      421 tgttgatcct ttaggtatct ttccacagcc aggattcttg cctggagctg cttgatgccc
      481 cagactgtga gttgcaacag atgctgttgc gcctcaatag ccctcagcaa attgttctgc
      541 tgctgcacta taccagacaa taattgtctg gcctgtaccg tcagcgtcat tgacgctgcg
      601 cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca aagctcctat tcccactgct
      661 cttttttctc tctgcaccac tcttctcttt gccttggtgg gtgctactcc taatggttca
      721 atttttacta ctttatattt atataattca cttctccaat tgtccctcat atctcctcct
      781 ccaggtctga agatcagcgg ccggccgctt gctgtgcggt ggtcttactt ttgttttgct
      841 cttcctctat cttgtctaaa gcttccttgg tgtcttttat ctctatcctt tgatgcacac
      901 aatagagggt tgctactgta ttatataatg atctaagttc ttctgatcct gtctgaaggg
      961 atggttgtag ctgtcccagt atttgtctac agccttctga tgtttctaac aggccaggat
     1021 taactgcgaa tcgttctagc tccctgcttg cccatactat atgttttaat ttatattttt
     1081 tctttccccc tggccttaac cgaatttttt cccatcgcga tctaattctc ccccgcttaa
     1141 tactgacgct ctcgcaccca tctctctcct tctagcctcc gctagtcaaa atttttggcg
     1201 tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc ttcagcaagc cgagtcctgc
     1261 gtcgagagag ctcctctggt ttccctttcg ctttcaagtc cctgttcggg cgccactgct
     1321 agagattttc cacactgact aaaagggtct gagggatctc tagttaccag agtcacacaa
     1381 cagacgggca cacactactt gaagcactca aggcaagctt tattgaggct taagcagtgg
     1441 gttccctagt tagccagaga gctcccaggc tcagatctgg tctaaccaga gagacccagt
     1501 acaggcaaaa agcagctgct tatatgcagg atctgagggc tcgccactcc ccagtcccgc
     1561 ccaggccacg cctccctgga aagtccccag cggaaagtcc cttgtagcaa gctcgatatc
     1621 agcagttctt gaagtactcc ggatgcagct ctcgggccac gtgatgaaat gctaggcggc
     1681 tgtcaaacct ccactctaac acttctctct ccgggtcatc catcccatgc aggctcacag
     1741 ggtgtaacaa gctggtgttc tctcctttat tggcctcttc taccttatct ggctcaactg
     1801 gtactagctt gtagcaccat ccaaaggtca gtggatatct gacccctggc cctggtgtgt
     1861 agttctgcta atcagggaag tagccttgtg tgtggtagat ccacagatca aggatatctt
     1921 gtcttctttg ggagtgaatt agcccttcca actactaagt ttgtagtaca tatttaacaa
     1981 atacaatttc tttaaaatga aaataattca gaggaatcac aggtttagag taaatgaaac
     2041 cacaggtaat tggcagtggt aatagggtat ggggtgggaa gtttgggatg attttggtta
     2101 gcttgagtta tccagttgat ccagacatga taagatacat tgatgagttt ggacaaacca
     2161 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     2221 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     2281 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     2341 gtatggctga ttatgatctc tagtcaaggc actatacatc aaatattcct tattaacccc
     2401 tttacaaatt aaaaagctaa aggtacacaa tttttgagca tagttattaa tagcagacac
     2461 tctatgcctg tgtggagtaa gaaaaaacag tatgttatga ttataactgt tatgcctact
     2521 tataaaggtt acagaatatt tttccataat tttcttgtat agcagtgcag ctttttcctt
     2581 tgtggtgtaa atagcaaagc aagcaagagt tctattacta aacacagcat gactcaaaaa
     2641 acttagcaat tctgaaggaa agtccttggg gtcttctacc tttctcttct tttttggagg
     2701 agtagaatgt tgagagtcag cagtagcctc atcatcacta gatggcattt cttctgagca
     2761 aaacaggttt tcctcattaa aggcattcca ccactgctcc cattcatcag ttccataggt
     2821 tggaatctaa aatacacaaa caattagaat cagtagttta acacattata cacttaaaaa
     2881 ttttatattt accttagagc tttaaatctc tgtaggtagt ttgtccaatt atgtcacacc
     2941 acagaagtaa ggttccttca caaagatccg gggcccactc ataaatccag ttgccgccac
     3001 ggtagccaat caccgtatcg tataaatcat cgtcggtacg ttcggcatcg ctcatcacaa
     3061 tacgtgcctg gacgtcgagg atttcgcgtg ggtcaatgcc gcgccagatc cacatcagac
     3121 ggttaatcat gcgataccag tgagggatgg ttttaccatc aagggccgac tgcacaggcg
     3181 gttgtgcgcc gtgattaaag cggcggacta gcgtcgaggt ttcaggatgt ttaaagcggg
     3241 gtttgaacag ggtttcgctc aggtttgcct gtgtcatgga tgcagcctcc agaatactta
     3301 ctggaaacta ttgtaacccg cctgaagtta aaaagaacaa cgcccggcag tgccaggcgt
     3361 tgaaaagatt agcgaccgga gattggcggg acgaatacga cgcccatatc ccacggctgt
     3421 tcaatccagg tatcttgcgg gatatcaaca acatagtcat caaccagcgg acgaccagcc
     3481 ggttttgcga agatggtgac aaagtgcgct tttggataca tttcacgaat cgcaaccgca
     3541 gtaccaccgg tatccaccag gtcatcaata acgatgaagc cttcgccatc gccttctgcg
     3601 cgtttcagca ctttaagctc gcgctggttg tcgtgatcgt agctggaaat acaaacggta
     3661 tcgacatgac gaatacccag ttcacgcgcc agtaacgcac ccggtacaga ccgccacggc
     3721 ttacggcaat aatgcctttc cattgttcag aaggcatcag tcggcttgcg agtttacgtg
     3781 catggatctg caacatgtcc caggtgacga tgtatttttc gctcatgtga agtgtcccag
     3841 cctgtttatc tacggcttaa aaagtgttcg aggggaaaat aggttgcgcg agattataga
     3901 gatctggcgc actaaaaacc agtatttcac atgagtccgc gtctttttac gcactgcctc
     3961 tccctgacgc gggataaagt ggtattctca aacatatctc gcaagcctgt cttgtgtcca
     4021 agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca
     4081 gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga
     4141 gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg
     4201 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
     4261 tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg
     4321 gagcctgggg actttccaca ccctaactga cacacattcc acagctgcat taatgaatcg
     4381 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     4441 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     4501 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     4561 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     4621 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     4681 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     4741 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     4801 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     4861 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     4921 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     4981 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     5041 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     5101 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     5161 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     5221 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     5281 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     5341 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     5401 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     5461 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     5521 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     5581 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     5641 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     5701 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     5761 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     5821 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     5881 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     5941 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     6001 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     6061 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     6121 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     6181 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     6241 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     6301 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     6361 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     6421 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     6481 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     6541 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     6601 accattcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg
     6661 aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt
     6721 cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag
     6781 tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct
     6841 gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatctggct agcgatgacc
     6901 ctgctgattg gttcgctgac catttccggg tgcgggacgg cgttaccaga aactcagaag
     6961 gttcgtccaa ccaaaccgac tctgacggca gtttacgaga gagatgatag ggtctgcttc
     7021 agtaagccag atgctacaca attaggcttg tacatattgt cgttagaacg cggctacaat
     7081 taatacataa ccttatgtat catacacata cgatttaggt gacactatag aatacacgga
     7141 attaattcta gatgctgcta gagattttcc acactgacta aaagggtctg agggatctct
     7201 agttaccaga gtcacacaac agacgggcac acactacttg aagcactcaa ggcaagcttt
     7261 attgaggctt aagcagtggg ttccctagtt agccagagag ctcccaggct cagatctggt
     7321 ctaaccagag agacccagta caagcaaaaa gcagatcttg tcttcgttgg gagtgaatta
     7381 gcccttccag tccccccttt tcttttaaaa agtggctaag atctacagct gccttgtaag
     7441 tcattggtct taaaggtacc gagctcgaat taattccctc gaggtcgacg gtatcgatgc
     7501 ggggaggcgg cccaaaggga gatccgactc gtctgagggc gaaggcgaag acgcggaaga
     7561 ggccgcagag ccggcagcag gccgcgggaa ggaaggtccg ctggattgag ggccgaaggg
     7621 acgtagcaga aggacgtccc gcgcagaatc caggtggcaa cacaggcgag cagccaagga
     7681 aaggacgatg atttccccga caacaccacg gaattgtcag tgcccaacag ccgagcccct
     7741 gtccagcagc gggcaaggca ggcggcgatg agttccgccg tggcaatagg gagggggaaa
     7801 gcgaaagtcc cggaaaggag ctgacaggtg gtggcaatgc cccaaccagt gggggttgcg
     7861 tcagcaaaca cagtgcacac cacgccacgt tgcctgacaa cgggccacaa ctcctcataa
     7921 agagacagca accaggattt atacaaggag gagaaaatga aagccatacg ggaagcaata
     7981 gcatgataca aaggcattaa agcagcgtat ccacatagcg taaaaggagc aacatagtta
     8041 agaataccag tcaatctttc acaaattttg taatccagag gttgattatc gataagcttg
     8101 atatcaagct tgcatgcctg caggttaggt gctgtccagg cccagcaggg ggttggggat
     8161 gggcttgcca ctagtggtag cacctgcagc gcttgacttc aaatcaacca ccaccacact
     8221 tatgttgtct ttgcttcctc tctgtatcgc cagctttgac aaatactcag ccgcggacat
     8281 cgccgcagga tctttccctt cctttctccg ctcatccgcg agcaacgatg catccccagc
     8341 caccgcgttt ttcttgtgcc acaagagaat ccgcttcctt gccatctcac acgcttcttc
     8401 atccgtcatt acatcccaaa ccccgtcact cgccaaaatc agacaatcat cttcttttac
     8461 tctcttcaca gccgtcactt ccggatcagg aatgatggat ggtttcaagt atctatcgcc
     8521 aatggatctc gacatggcga gaacaccgaa aacacgagct ccattccact gaatcacttt
     8581 ccctcctgcg gcttcaatcc tcgcagcttc atcttctcta tccggtttat ggtcaacgga
     8641 taatggaagt gcagttttgc cgcggcaaag aacggctcta gagtcaccgc agttagcgac
     8701 gaagatgtga gacgggaaaa caacggcaac caccgacgtt gacccaaccg tctccggcgc
     8761 aactgactca atctccgagt caactctcag gaacgagttg aaaagagctt tcttccactt
     8821 ctccagccac gtatcaccat cgcagagcat cggtttctcc ttagctatct cctccgccaa
     8881 agccaaatgc atcctctctc tacaatagtt cgctacctga gaaccgccat ggccgtcgta
     8941 aacaccgaag aaatgagcgg cggattgagg atcaaaccga ccatctaaca tcgaaccaga
     9001 ggaagattga aggaatcttg gtatagtcga aacagcagct tccatctcag gtcttcttcc
     9061 acaaatcgaa gtaaaaccat acaaaggcac acgcgtggat ccggatccca tatgcttgta
     9121 cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg
     9181 atcgcgcttc tcgttggggt ctttgctcag ggcggactgg gtgctcaggt agtggttgtc
     9241 gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg
     9301 cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc cgttcttctg
     9361 cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct tgtgccccag
     9421 gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc
     9481 gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt
     9541 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
     9601 gtcggggtag cggctgaagc actgcacgcc gtaggtcagg gtggtcacga gggtgggcca
     9661 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc
     9721 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc
     9781 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacgg tggcgaccgg
     9841 tagcgcctct agagctccct ccgcggccgc cttcatgatg gcgtactccc caaaggcctt
     9901 cttgatgctc atggcgatct caggcagggc cttgatttcc accacgcctt tgtcggggta
     9961 attgtttgca tcgaaggcgt tccggatctt ccgcaggatg tcgctctgct ctttgttgat
    10021 gttcttgttg ttcagcagga ttttcaggat gctcttgaag tccaccttct cttcccggtc
    10081 cactctggca gacagctcgg ggtatgtgtc gaaggcccac ttttccaggt tgaacacgat
    10141 ggagctgatc tcgggcctgc actggtcgta tttgttgaac tcttccatga tatcctcttt
    10201 ggacacgatg tcgctgccca cgagttccag caggttgccg atcctcttgt cgctagccag
    10261 cacaaagaag tcgccgtagt tcttcagctt catgccctcg ctggtgatgg tgtacttctt
    10321 gccgcctttc tcgaaggtga agctcatggg catgatctcg ctcaggattc ccttctcggc
    10381 gtcgggcatg atttctttca gtttgaacct ctcgccgtcg aaatcggcca gttcggtcag
    10441 ggtctttttg gccagcagaa acagcagggc atcctgcact ctgtagcgcc ggatcacttt
    10501 ctcgcttttc tggtactcgt tccggctgtt gctcagccgc ttatccagga ttgtctcgat
    10561 ctcttcgctg ctggcgtttc tcatctgccg gttgctgcgg atcttgttgc tggcctgctt
    10621 tctgtaccgc tctgttctgg aggcccgctc tttccagagg ccttctctct cttccacgct
    10681 ggtgaagcag tgctgcaggg agccctttct gtcgtactcg cccttaagca tgtccatgta
    10741 ccggtagttg cggttccact ggtagaaggt ctggaagtcg tcgtccagca ctctcttcat
    10801 gtactcggcg atcagatagg tcacgttggc attgttgaag tcgatgcctt ccatctgtgg
    10861 cagggacttc aggtgggact tgatctcatt gtcgaacatc tgtctgggca gttccacggg
    10921 cagatcctcg ctgtagattc tgcccagggt cttcatggcg ggtgttttcc acttgttctg
    10981 gtcccgccgg atgaagggca catccactct gttgcctttc ttgatctcgt tggacaggcc
    11041 ggtcaggtag aacttccgct cgatcaggta gcgctcgtag aactcgacgg cattggcggg
    11101 gatgctgcgg gcgaacacct tgtacagaaa tggatgaggc tctgttgtgc ccttgccgat
    11161 cagccgggcc ttctcgaaca tcagcttgaa ctgctgcttg gcctcgtaat cgtcgccgct
    11221 atcgtacacg gcaatggcgc tctgcatgat ccggtagttc aggccggtga tcttgttctc
    11281 gccatcgttc acgctgggct gaaacagcac gatgtccttg gccaggaagt cggccagctt
    11341 gcctgtggag atctgcttga agcctctctt tcccatcttg ttgtcggcgc tccgaatgga
    11401 cttccggtcg tccttgaatc tcttgattct ccgctcggtg tcggtcagca tgtcgtccac
    11461 ggtcagtctg atgaaggcgt ccacatcctt gccgtgggca ttgccgctga tcagatccag
    11521 gatcttctga ggcaggtcgc tctcggcgat tccgaagctg gcgatattct cggcggtcac
    11581 ttcttctttc tgcatggcct ggaacagtct cttgtaccgg ttgtgcacgt ccacgatcag
    11641 cttctcggtt ttcttgctgc cgaacagaaa catgtggaag gccatggctg gaatttccag
    11701 ggtgctcatc cggcagctgg ggattgtctt gaccacgtat ctatcatcct cgatcacggg
    11761 cagcagtggg gcgctgtcct ctttgtcgtt gataaacatc tcgaccttgt tgttttccag
    11821 gatgtagtgt gtgtaggtgt ccacgatgta gggatagttg gcaggattgg cgtcgtcccg
    11881 cttcatgttc tcgaagtctc tgatccggat gccgctgttg ccgaaggtgc cgttctcttg
    11941 cttccgcatt gtctcggcct cttccagtct gccgaagccg ttcaggggct gctcgatcac
    12001 tctgactctg gtctggccgt cgatgcaggt cttgtcggcc ttcagcaggt atctcagctt
    12061 gcccatgttc acgtggaacc tgatgtggtc gaacagcttg ccgtaatcga tatactgcag
    12121 cagcagaggc acgaatctgt cgctgctccg cttcatcagc acttcattgt ggtcgtcgct
    12181 gatgattctg aaccgggact gcttctcggc agacagtgtt gtgaacagct cgtcggggca
    12241 ccgcttcact tcgttgagca tatccatggc cacgctcttg ttggacttct cgctgtggat
    12301 ccggtccttg ggcagcttga tgctgttgat gccgaaggat ctgatgatga tccgccgttc
    12361 ctcgctctgg gcattgtagc tggagaagat gggcagcctg ctcagaaaga tgttgatgta
    12421 ctgcttgtcc aggaacaggc agatcagcag ggcgattccc actccgctca ggtgcagctt
    12481 cttctgtgtg tcgccgttgt agtcctgcag gctcaggaag aatccggtat tcacttggga
    12541 ctttttcttg ccgtaggcgt ccttcacgaa cttgaaccgc ttgtcctgga tgaaggccag
    12601 gtcctctgtc ttgtagccgt atctctcgtt catgttccgc agggccactg tgtagtagtt
    12661 gttgatcatg ccgctcagag gttgctctgt gctggtcagg aactcgcagc cgtcgttcag
    12721 cttttcctcg taggtcttgt atgcgttggt caggtccctg tacatcttca gcacgccgaa
    12781 ggcgcgcttc agcacctcga agatgtcgtt gctgttcact tccacgcggt tctgcttgta
    12841 cttgccgttg ctgtactctc tctggttctc ggccatgatc ttcaggaatg ggaagtagct
    12901 ctgcagccgc tcgatgatga acatggtttt ctcgggctgc ttgtcgtagc cgttcttggc
    12961 gttgtacagg tggctcatca cggggtgaaa ccacagattc tcgttgttct cgttctgctc
    13021 gccctcaata tcggccacct tctggatgtg gtccagcacg gtctgagcgt tcagcatggc
    13081 catcacgctg taggtgccaa agtacttctt ctggttttcc accagagcgg ggatgttctc
    13141 gagggatccg gtcaggatcc gccgcagccg ctcagggtac agcggtccgc cggatccctt
    13201 gtcatcgtcg tccttgtaat ccatggtggc acgcgtgaga attcctgaaa tggaagaaaa
    13261 aaactttgaa ccactgtctg aggcttgaga atgaaccaag atccaaactc aaaaagggca
    13321 aattccaagg agaattacat caagtgccaa gctggcctaa cttcagtctc cacccactca
    13381 gtgtggggaa actccatcgc ataaaacccc tccccccaac ctaaagacga cgtactccaa
    13441 aagcgcgaga actaatcgag gtgcctggac ggcgcccggt actccgtgga gtcacatgaa
    13501 gcgacggctg aggacggaaa ggcccttttc ctttgtgtgg gtgactcacc cgcccgctct
    13561 cccgagcgcc gcgtcctcca ttttgagctc cctgcagcag ggccgggaag cggccatctt
    13621 tccgctcacg caactggtgc cgaccgggcc agccttgccg cccagggcgg ggcgatacac
    13681 ggcggcgcga ggccaggcac cagagcaggc cggccagctt gagactaccc ccgtccgatt
    13741 ctcggtggcc gcgctcgcag gccccgcctc gccgaacatg tgcgctggga cgcacgggcc
    13801 ccgtcgccgc ccgcggcccc aaaaaccgaa ataccagtgt gcagatcttg gcccgcattt
    13861 acaagactat cttgccagaa aaaaagcgtc gcagcaggtc atcaaaaatt ttaaatggct
    13921 agagacttat cgaaagcagc gagacaggcg cgaaggtgcc accagattcg cacgcggcgg
    13981 ccccagcgcc caggccaggc ctcaactcaa gcacgaggcg aaggggctcc ttaagcgcaa
    14041 ggcctcgaac tctcccaccc acttccaacc cgaagctcgg gatcaagaat cacgtactgc
    14101 agccaggtgg aagtaattca aggcacgcaa gggccataac ccgtaaagag gccaggcccg
    14161 cgggaaccac acacggcact tacctgtgtt ctggcggcaa acccgttgcg aaaaagaacg
    14221 ttcacggcga ctactgcact tatatacggt tctcccccac cctcgggaaa aaggcggagc
    14281 cagtacacga catcactttc ccagtttacc ccgcgccacc ttctctaggc accggttcaa
    14341 ttgccgaccc ctccccccaa cttctcgggg actgtgggcg atgtgcgctc tgcccactga
    14401 cgggcacgaa ttcgatatca agcttatcga tccaaactgg atctctgctg tccctgtaat
    14461 aaacccgaaa attttgaatt tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg
    14521 ttgctattat gtctactatt ctttcccctg cactgtaccc cccaatcccc ccttttcttt
    14581 taaaattgtg gatgaatact gccatttggc gataccgtcg agatccgttc actaatcgaa
    14641 tggatctgtc tctgtctctc tctccacctt cttcttctat tcct
//
