LOCUS       sequence-474750-        5975 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A mammalian expression plasmid encoding Arl13b with mcherry at 
            C-terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5975)
  AUTHORS   Madugula V, Lu L
  TITLE     A ternary complex comprising transportin1, Rab8 and the ciliary 
            targeting signal directs proteins to ciliary membranes.
  JOURNAL   J Cell Sci. 2016 Oct 15;129(20):3922-3934. Epub 2016 Sep 15.
  PUBMED    27633000
REFERENCE   2  (bases 1 to 5975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2016-10-15"; volume: "129(20)"; pages: "3922-3934. 
            Epub 2016 Sep 15"
COMMENT     Sequence Label: Arl13b-mcherry
FEATURES             Location/Qualifiers
     source          1..5975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1924..1933
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1930..2640
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2075..2093)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2343..2362
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    2763..2884
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2800..2819)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2854..2873
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2891..3346)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3028..3049)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3240..3259
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3373..3477
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3479..3836
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3479..3670
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3502..3522)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3687..3822
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3871..4665
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3871..4665
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3925..3944)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4535..4554
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4853..4872)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4897..4944
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5273..5861
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5762..5781
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgaggc caccatgttc agtctgatgg ccagttgctg cggctggttc
      661 aagcggtggc gggagcctgt cagaaaggtg actcttttga tggtgggact tgataatgct
      721 ggtaaaaccg caacagcaaa gggaatccaa ggagaatacc ctgaagatgt agctcctact
      781 gttggatttt caaaaattaa ccttagacaa ggaaagtttg aagtcaccat ctttgacttg
      841 ggaggtggaa taagaattcg gggaatctgg aagaattact atgctgaatc ctatggggta
      901 atatttgttg tggattccag tgatgaagag agaatggaag agacaaaaga ggctatgtca
      961 gaaatgctaa gacatcctag gatatcggga aagcctatat tggtgttggc aaataaacaa
     1021 gataaagaag gagctttagg agaagctgat gtcattgaat gtctatctct ggaaaaattg
     1081 gtcaatgagc acaagtgcct gtgtcagata gaaccatgtt cagcaatctc ggggtatgga
     1141 aagaaaattg acaagtccat taaaaaaggc ctttattggc tgctacatgt tattgcaaga
     1201 gactttgatg ccttaaatga acgcatccaa aaagagacaa cagagcagcg tgctcttgag
     1261 gaacaagaga aacaagaaag agctgaacga gtgcgaaaat tacgagaaga aagaaaacaa
     1321 aatgaacagg agcaggctga actcgatgga accagtggtc tggctgagtt ggacccagaa
     1381 ccaacgaatc ctttccagcc aatagcatct gtaatcattg agaacgaagg aaaacttgaa
     1441 agagagaaaa aaaaccaaaa aatggagaaa gacagtgatg gctgccacct gaaacataaa
     1501 atggagcatg agcaaataga gacacaaggc caggttaatc acaatggcca aaaaaataat
     1561 gaatttggac tagtagaaaa ttataaggag gcattaacac agcagttaaa gaatgaagat
     1621 gagacagacc ggccatcatt ggaatcagct aatggtaaaa agaaaactaa gaaactaaga
     1681 atgaaaagga accaccgggt agaaccactt aatatagatg actgtgctcc tgagagtcca
     1741 acgccacccc caccccctcc tcctgttggc tggggaaccc ctaaagtcac tagacttcca
     1801 aaacttgagc ctcttggtga aacacatcat aatgatttct ataggaagcc actgcctccc
     1861 ctggctgtgc cacagcgacc taacagtgat gctcatgatg tgatctcatt ggatccaccg
     1921 gtcgccacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     1981 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     2041 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     2101 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     2161 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     2221 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     2281 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     2341 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     2401 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     2461 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     2521 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     2581 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtag
     2641 agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     2701 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     2761 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     2821 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     2881 cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     2941 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     3001 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     3061 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     3121 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     3181 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     3241 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     3301 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt
     3361 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     3421 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct
     3481 gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     3541 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     3601 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     3661 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     3721 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     3781 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc
     3841 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     3901 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     3961 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     4021 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg
     4081 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     4141 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     4201 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     4261 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     4321 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     4381 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     4441 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     4501 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     4561 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     4621 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     4681 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     4741 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     4801 agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac
     4861 ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa
     4921 cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg
     4981 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac
     5041 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc
     5101 ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat
     5161 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     5221 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     5281 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     5341 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     5401 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     5461 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     5521 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     5581 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     5641 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     5701 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     5761 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     5821 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     5881 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     5941 ctgattctgt ggataaccgt attaccgcca tgcat
//
