LOCUS       sequence-474755-       11615 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses PYL1-DDX6 for recruiting RNA to the p-body via CRISPR-TO.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11615)
  AUTHORS   Han M, Fu ML, Zhu Y, Choi AA, Li E, Bezney J, Cai S, Miles L, Ma Y, 
            Qi LS
  TITLE     Programmable control of spatial transcriptome in live cells and 
            neurons.
  JOURNAL   Nature. 2025 Jul;643(8070):241-251. doi: 10.1038/s41586-025-09020-z.
            Epub 2025 May 21.
  PUBMED    40399675
REFERENCE   2  (bases 1 to 11615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-025-09020-z"; journalName: "Nature"; date: 
            "2025-07"; volume: "643"; issue: "8070"; pages: "241-251"
COMMENT     Sequence Label: pSLQ10539
FEATURES             Location/Qualifiers
     source          1..11615
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     169..185
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     170..186
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    189..777
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             295..306
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     704..724
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2282..2977)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     promoter        complement(3529..3936)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    4089..4206
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(4491..4535)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4720..4953
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5450..5575
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5622..6255
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6423..6557
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6447..6466)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6501..6520
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6982..7002)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          7132..7197
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(8331..8660)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8345..8480
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8645..8665
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(8735..8752)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8906..9494)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8986..9005)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9665..10456,10457..10525))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10288..10307
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10526..10630)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10698..10716
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10754..10776)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10876..10895
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11109..11128
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        11418..11436
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     11418..11435
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             join(11461..11615,1..79)
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 cagatctggt ctaaccagag agacccagta caagcaaaaa gcagatcttg tcttcgttgg
       61 gagtgaatta gcccttccag tccccccttt tcttttaaaa agtggctaag atctacagct
      121 gccttgtaag tcattggtct taaaggtacc gagctcgaat taattccctc gaggtcgacg
      181 gtatcgatgc ggggaggcgg cccaaaggga gatccgactc gtctgagggc gaaggcgaag
      241 acgcggaaga ggccgcagag ccggcagcag gccgcgggaa ggaaggtccg ctggattgag
      301 ggccgaaggg acgtagcaga aggacgtccc gcgcagaatc caggtggcaa cacaggcgag
      361 cagccaagga aaggacgatg atttccccga caacaccacg gaattgtcag tgcccaacag
      421 ccgagcccct gtccagcagc gggcaaggca ggcggcgatg agttccgccg tggcaatagg
      481 gagggggaaa gcgaaagtcc cggaaaggag ctgacaggtg gtggcaatgc cccaaccagt
      541 gggggttgcg tcagcaaaca cagtgcacac cacgccacgt tgcctgacaa cgggccacaa
      601 ctcctcataa agagacagca accaggattt atacaaggag gagaaaatga aagccatacg
      661 ggaagcaata gcatgataca aaggcattaa agcagcgtat ccacatagcg taaaaggagc
      721 aacatagtta agaataccag tcaatctttc acaaattttg taatccagag gttgattatc
      781 gataagcttg atatcaagct tgcatgcctg caggtcaagg tttctcatct tctacaggct
      841 cgctgtggta ttctgccaca tacaggctct tatcaatgtt gctcggaata ggtttaattt
      901 ctgttcccag ctgctcctca atacttttca ggttgaagcg atcatcatat gtgatcaagt
      961 tgatggctaa gccaagatga ccaaagcgac ctgatcttcc aatacgatgg agataggtct
     1021 ctgccagctt tgggaaatca aagtttatta ccacattcac agcttgtata tcaatacctc
     1081 gggtaaacag atcagtgcaa acaagattgc ggcataagcc atttcggaaa tcatgaaata
     1141 cacgatttcg atgttcctgc ctcattttag catgaatata gaagcaagaa taacccagtt
     1201 gagaaatctt cttggctagc aattcaactc gctgagagga gttacagaaa atgatcgact
     1261 ggtttatctg aagcctggag aaaagtgtgt tgaggcagtg tactttttgg cgctcagtta
     1321 catatgcgta gtactgggtt actcccttca gagttagttc ctccatcagg ttaatctcat
     1381 agggtttctg caaatgggaa ttcatgaact tctgtacact aagagggaaa gtagcggaat
     1441 atagtaaaat ctgcctgttt ttaggtagcg tgagaataat atcctccatt atctgcacaa
     1501 aatcctgtga cagcaactta tctgcctcat ccaatactat catctggaca tgatcaacct
     1561 ttgctactcc tttcttaata agatccagga ttctcccagg ggtagcaatc accacgtgca
     1621 ctgtatcatc aagcctcatt atgtcatctc gtaaattggt tcctcctgtg gttgccatca
     1681 ctttggcccc tcccatgtgt ttgctgacct ggatgcaaat ttgactgacc tgtagagcaa
     1741 gttctctagt gggaacaatc accattgctt gtatattgtc cttcttcagg tctagccgtt
     1801 caagtaaggg aatgaggtag gcaccgctct tgcctgttcc attttttgct ctagctaaga
     1861 tatccctacc agataaagca atgggaatgc tctcctcctg aataggagat ggcttttccc
     1921 agcccatttc aaaaattccc atcagtaact cccgtttcaa acagtaatct tcaaactcat
     1981 ttccttttgt ggaggtcaca tccgaagttt tgattcttag atcctttgga gggagtttta
     2041 aagtcttttt ccagtcatca ccaggtttaa tagtggtggt catactctgt gcttgctgct
     2101 gagtgccatt attgattgtg ttggtgtttt tcagctggtt catctgttgc tgtgtctgtg
     2161 tgccccctcc tccagggcca ccagtgggtt tcacagggcc tctcagctga ccattttgac
     2221 tggacagacc cattataaca gggttctctg ttctggccgt gctcatcttg cttgcggtcc
     2281 gattaagctt gtgccccagt ttgctaggga ggtcgcagta tctggccact gccacctcgt
     2341 gctgctcgac gtaggtctcg ttgttggcct ccttgattct ttccagtctg tagtccacat
     2401 agtagacgcc aggcatcttg aggttcttag cgggtttctt ggatctatat gtggtcttga
     2461 tgtttgcgat cagatggctc ccgcccacga gcttcagggc catgtcgttt ctgccttcca
     2521 ggccgccgtc agcggggtac agcgtctcgg tgaaggcctc ccagccgagt gttttcttct
     2581 gcatcacagg gccgttggat gtgaagttca cccctctgat cttgacgttg tagatgaggc
     2641 agccgtcctg gaggctggtg tcctgggtag cggtcagcac gcccccgtct tcgtatgtgg
     2701 tgactctctc ccatgtgaag ccctcaggga aggactgctt gaagaagtcg gggatgccct
     2761 gggtgtggtt gatgaaggtc ttgctgccgt agaggaagct agtagccagg atgtcgaagg
     2821 cgaaggggag agggccgccc tcgaccacct tgattctcat ggtctgggtg ccctcgtagg
     2881 gcttgccttc gccctcggat gtgcacttga agtgatggtt gtccacggtg ccctccatgt
     2941 acagcttcat gtgcatgttc tccttaatca gctcgctcgg atccacgttc atagcttcag
     3001 tgatcgaagc aagtttctga agattcaatc taataaccgt atcagcaaac aatctcgtat
     3061 cttcctccga attaccttcc ggtacatcaa caacataaga ttccaaaaca acggtccaga
     3121 tcctttcttc ttcttcttct ttctcaaatc tatgaaccgt cgtaaccgat ttataattcc
     3181 tcagcctatg ttcaccaccg gttatactaa acccagtcac tctccgatca tcgtccaaca
     3241 gatctaatct ctctcgagac gtattcgccg gtaatccact tatcacgttc acgtcgcgcg
     3301 tgcatcccac tcgcatctcg aaatcttcac tcacgttaca gcttttgatg aagtgtttgt
     3361 aaatctgtgg cctatcgaaa cgtctcacca cggaccatac tgtttccggc ggcgcgtgga
     3421 ttcgctgagc taggagagat gagcaacggc cgttaccgag ttggtacgtg tggaactcgg
     3481 cgattgattg ggagagttgg gtgaattcgt cttgagttgg cgcgccccca cccatgactc
     3541 agtctgtcgg aggactggcg cgccgagtga ggggttgtga gctcttttat agagctcggg
     3601 aagcagaagc gcgcgaacag aagcgagaag caggctgatt ggttaattca aataaggcgc
     3661 agggtcattt caggtccttg ggggagcctg gaaacatctg atgggtctta agaaactgct
     3721 gagggttggg ccatatctgg ggaccatctg ttcttggcct cgggccgggg ccgaaactgc
     3781 ggtgaccatc tgttcttggc cccgggccgg ggccgaaact gctcaccgca gatatcctgt
     3841 ttggcccaac gttagctatt ttcatgtacc cgcccttgat ctgaacttct ctattcttgg
     3901 tttggtattt ttccatgcct tgcaaaatgg cgttactgca gctagcttgc caaacctaca
     3961 ggtggggtct ttcattcccc cctttttctg gagactaaat aaaatctttt attttatcgg
     4021 ggctgcagga attcgatatc aagcttatcg atccaaactg gatctctgct gtccctgtaa
     4081 taaacccgaa aattttgaat ttttgtaatt tgtttttgta attctttagt ttgtatgtct
     4141 gttgctatta tgtctactat tctttcccct gcactgtacc ccccaatccc cccttttctt
     4201 ttaaaattgt ggatgaatac tgccatttgg cgataccgtc gagatccgtt cactaatcga
     4261 atggatctgt ctctgtctct ctctccacct tcttcttcta ttccttcggg cctgtcgggt
     4321 cccctcgggg ttgggaggtg ggtctgaaac gataatggtg aatatccctg cctaactcta
     4381 ttcactatag aaagtacagc aaaaactatt cttaaaccta ccaagcctcc tactatcatt
     4441 atgaataatt ttatatacca cagccaattt gttatgttaa accaattcca caaacttgcc
     4501 catttatcta attccaataa ttcttgttca ttcttttctt gctggttttg cgattcttca
     4561 attaaggagt gtattaagct tgtgtaattg ttaatttctc tgtcccactc catccaggtc
     4621 gtgtgattcc aaatctgttc cagagattta ttactccaac tagcattcca aggcacagca
     4681 gtggtgcaaa tgagttttcc agagcaaccc caaatcccca ggagctgttg atcctttagg
     4741 tatctttcca cagccaggat tcttgcctgg agctgcttga tgccccagac tgtgagttgc
     4801 aacagatgct gttgcgcctc aatagccctc agcaaattgt tctgctgctg cactatacca
     4861 gacaataatt gtctggcctg taccgtcagc gtcattgacg ctgcgcccat agtgcttcct
     4921 gctgctccca agaacccaag gaacaaagct cctattccca ctgctctttt ttctctctgc
     4981 accactcttc tctttgcctt ggtgggtgct actcctaatg gttcaatttt tactacttta
     5041 tatttatata attcacttct ccaattgtcc ctcatatctc ctcctccagg tctgaagatc
     5101 agcggccggc cgcttgctgt gcggtggtct tacttttgtt ttgctcttcc tctatcttgt
     5161 ctaaagcttc cttggtgtct tttatctcta tcctttgatg cacacaatag agggttgcta
     5221 ctgtattata taatgatcta agttcttctg atcctgtctg aagggatggt tgtagctgtc
     5281 ccagtatttg tctacagcct tctgatgttt ctaacaggcc aggattaact gcgaatcgtt
     5341 ctagctccct gcttgcccat actatatgtt ttaatttata ttttttcttt ccccctggcc
     5401 ttaaccgaat tttttcccat cgcgatctaa ttctcccccg cttaatactg acgctctcgc
     5461 acccatctct ctccttctag cctccgctag tcaaaatttt tggcgtactc accagtcgcc
     5521 gcccctcgcc tcttgccgtg cgcgcttcag caagccgagt cctgcgtcga gagagctcct
     5581 ctggtttccc tttcgctttc aagtccctgt tcgggcgcca ctgctagaga ttttccacac
     5641 tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac
     5701 tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc
     5761 agagagctcc caggctcaga tctggtctaa ccagagagac ccagtacagg caaaaagcag
     5821 ctgcttatat gcaggatctg agggctcgcc actccccagt cccgcccagg ccacgcctcc
     5881 ctggaaagtc cccagcggaa agtcccttgt agcaagctcg atatcagcag ttcttgaagt
     5941 actccggatg cagctctcgg gccacgtgat gaaatgctag gcggctgtca aacctccact
     6001 ctaacacttc tctctccggg tcatccatcc catgcaggct cacagggtgt aacaagctgg
     6061 tgttctctcc tttattggcc tcttctacct tatctggctc aactggtact agcttgtagc
     6121 accatccaaa ggtcagtgga tatctgaccc ctggccctgg tgtgtagttc tgctaatcag
     6181 ggaagtagcc ttgtgtgtgg tagatccaca gatcaaggat atcttgtctt ctttgggagt
     6241 gaattagccc ttccaactac taagtttgta gtacatattt aacaaataca atttctttaa
     6301 aatgaaaata attcagagga atcacaggtt tagagtaaat gaaaccacag gtaattggca
     6361 gtggtaatag ggtatggggt gggaagtttg ggatgatttt ggttagcttg agttatccag
     6421 ttgatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     6481 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     6541 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     6601 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg
     6661 atctctagtc aaggcactat acatcaaata ttccttatta acccctttac aaattaaaaa
     6721 gctaaaggta cacaattttt gagcatagtt attaatagca gacactctat gcctgtgtgg
     6781 agtaagaaaa aacagtatgt tatgattata actgttatgc ctacttataa aggttacaga
     6841 atatttttcc ataattttct tgtatagcag tgcagctttt tcctttgtgg tgtaaatagc
     6901 aaagcaagca agagttctat tactaaacac agcatgactc aaaaaactta gcaattctga
     6961 aggaaagtcc ttggggtctt ctacctttct cttctttttt ggaggagtag aatgttgaga
     7021 gtcagcagta gcctcatcat cactagatgg catttcttct gagcaaaaca ggttttcctc
     7081 attaaaggca ttccaccact gctcccattc atcagttcca taggttggaa tctaaaatac
     7141 acaaacaatt agaatcagta gtttaacaca ttatacactt aaaaatttta tatttacctt
     7201 agagctttaa atctctgtag gtagtttgtc caattatgtc acaccacaga agtaaggttc
     7261 cttcacaaag atccggggcc cactcataaa tccagttgcc gccacggtag ccaatcaccg
     7321 tatcgtataa atcatcgtcg gtacgttcgg catcgctcat cacaatacgt gcctggacgt
     7381 cgaggatttc gcgtgggtca atgccgcgcc agatccacat cagacggtta atcatgcgat
     7441 accagtgagg gatggtttta ccatcaaggg ccgactgcac aggcggttgt gcgccgtgat
     7501 taaagcggcg gactagcgtc gaggtttcag gatgtttaaa gcggggtttg aacagggttt
     7561 cgctcaggtt tgcctgtgtc atggatgcag cctccagaat acttactgga aactattgta
     7621 acccgcctga agttaaaaag aacaacgccc ggcagtgcca ggcgttgaaa agattagcga
     7681 ccggagattg gcgggacgaa tacgacgccc atatcccacg gctgttcaat ccaggtatct
     7741 tgcgggatat caacaacata gtcatcaacc agcggacgac cagccggttt tgcgaagatg
     7801 gtgacaaagt gcgcttttgg atacatttca cgaatcgcaa ccgcagtacc accggtatcc
     7861 accaggtcat caataacgat gaagccttcg ccatcgcctt ctgcgcgttt cagcacttta
     7921 agctcgcgct ggttgtcgtg atcgtagctg gaaatacaaa cggtatcgac atgacgaata
     7981 cccagttcac gcgccagtaa cgcacccggt acagaccgcc acggcttacg gcaataatgc
     8041 ctttccattg ttcagaaggc atcagtcggc ttgcgagttt acgtgcatgg atctgcaaca
     8101 tgtcccaggt gacgatgtat ttttcgctca tgtgaagtgt cccagcctgt ttatctacgg
     8161 cttaaaaagt gttcgagggg aaaataggtt gcgcgagatt atagagatct ggcgcactaa
     8221 aaaccagtat ttcacatgag tccgcgtctt tttacgcact gcctctccct gacgcgggat
     8281 aaagtggtat tctcaaacat atctcgcaag cctgtcttgt gtccaagctt tttgcaaaag
     8341 cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc
     8401 tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg
     8461 ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg
     8521 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acacctggtt
     8581 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
     8641 ccacacccta actgacacac attccacagc tgcattaatg aatcggccga cgcgcgggga
     8701 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     8761 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     8821 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     8881 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     8941 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     9001 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     9061 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     9121 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     9181 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     9241 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     9301 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     9361 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     9421 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     9481 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     9541 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     9601 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     9661 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     9721 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     9781 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     9841 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     9901 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     9961 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    10021 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    10081 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    10141 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    10201 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    10261 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    10321 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    10381 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    10441 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    10501 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    10561 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    10621 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
    10681 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
    10741 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
    10801 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
    10861 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat tcgacgctct
    10921 cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc
    10981 gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg
    11041 cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct
    11101 tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg
    11161 ccggccacga tgcgtccggc gtagaggatc tggctagcga tgaccctgct gattggttcg
    11221 ctgaccattt ccgggtgcgg gacggcgtta ccagaaactc agaaggttcg tccaaccaaa
    11281 ccgactctga cggcagttta cgagagagat gatagggtct gcttcagtaa gccagatgct
    11341 acacaattag gcttgtacat attgtcgtta gaacgcggct acaattaata cataacctta
    11401 tgtatcatac acatacgatt taggtgacac tatagaatac acggaattaa ttctagatgc
    11461 tgctagagat tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac
    11521 acaacagacg ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca
    11581 gtgggttccc tagttagcca gagagctccc aggct
//
