LOCUS       sequence-474759-       10004 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the m6A-binding-deficient mutant of YTHDF2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10004)
  AUTHORS   Chen Z, Zeng C, Yang L, Che Y, Chen M, Sau L, Wang B, Zhou K, Chen 
            Y, Qing Y, Shen C, Zhang T, Wunderlich M, Wu D, Li W, Wang K, Leung 
            K, Sun M, Tang T, He X, Zhang L, Swaminathan S, Mulloy JC, Muschen 
            M, Huang H, Weng H, Xiao G, Deng X, Chen J
  TITLE     YTHDF2 promotes ATP synthesis and immune evasion in B cell 
            malignancies.
  JOURNAL   Cell. 2024 Dec 12:S0092-8674(24)01324-2. doi: 
            10.1016/j.cell.2024.11.007.
  PUBMED    39694037
REFERENCE   2  (bases 1 to 10004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Dec 12:S0092-8674(24)01324-2. doi: 10.1016/j.cell.2024.11.007."
COMMENT     Sequence Label: pCDH-3_Flag-YTHDF2-m6A-MUT
FEATURES             Location/Qualifiers
     source          1..10004
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(256..300)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    485..718
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1211..1336
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1383..1563
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1564..1790)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1805..1822)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1805..1821)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1814..1836)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2030..2049)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2149..2171
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2209..2227)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2295..2399
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2400..2468,2469..3260)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2618..2637)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3431..4019
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4173..4190
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4307..4328
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4343..4348,4349..4366,4367..4373)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4381..4397
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4386..4408
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4405..4421
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4405..4421
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      4454..4589
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4629..4750
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4640..4659)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4694..4713
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4822..5055
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    5129..5717
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5235..5246
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5644..5664
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5718..6317)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5801..5821)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     6298..6317
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(6318..6371)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(6441..7103)
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     regulatory      7124..7133
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     LTR             7162..7430
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(7443..7654)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             complement(9445..9510)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(9563..9587)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(9584..9787)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(9613..9633)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    9877..9993
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ccgtcgagat ccgttcacta atcgaatgga tctgtctctg tctctctctc caccttcttc
       61 ttctattcct tcgggcctgt cgggtcccct cggggttggg aggtgggtct gaaacgataa
      121 tggtgaatat ccctgcctaa ctctattcac tatagaaagt acagcaaaaa ctattcttaa
      181 acctaccaag cctcctacta tcattatgaa taattttata taccacagcc aatttgttat
      241 gttaaaccaa ttccacaaac ttgcccattt atctaattcc aataattctt gttcattctt
      301 ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt aattgttaat
      361 ttctctgtcc cactccatcc aggtcgtgtg attccaaatc tgttccagag atttattact
      421 ccaactagca ttccaaggca cagcagtggt gcaaatgagt tttccagagc aaccccaaat
      481 ccccaggagc tgttgatcct ttaggtatct ttccacagcc aggattcttg cctggagctg
      541 cttgatgccc cagactgtga gttgcaacag atgctgttgc gcctcaatag ccctcagcaa
      601 attgttctgc tgctgcacta taccagacaa taattgtctg gcctgtaccg tcagcgtcat
      661 tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca aagctcctat
      721 tcccactgct cttttttctc tctgcaccac tcttctcttt gccttggtgg gtgctactcc
      781 taatggttca atttttacta ctttatattt atataattca cttctccaat tgtccctcat
      841 atctcctcct ccaggtctga agatcagtgg ccgcttgctg tgcggtggtc ttacttttgt
      901 tttgctcttc ctctatcttg tctaaagctt ccttggtgtc ttttatctct atcctttgat
      961 gcacacaata gagggttgct actgtattat ataatgatct aagttcttct gatcctgtct
     1021 gaagggatgg ttgtagctgt cccagtattt gtctacagcc ttctgatgtt tctaacaggc
     1081 caggattaac tgcgaatcgt tctagctccc tgcttgccca tactatatgt tttaatttat
     1141 attttttctt tccccctggc cttaaccgaa ttttttccca tcgcgatcta attctccccc
     1201 gcttaatact gacgctctcg cacccatctc tctccttcta gcctccgcta gtcaaaattt
     1261 ttggcgtact caccagtcgc cgcccctcgc ctcttgccgt gcgcgcttca gcaagccgag
     1321 tcctgcgtcg agagagctcc tctggtttcc ctttcgcttt caagtccctg ttcgggcgcc
     1381 actgctagag attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc
     1441 acacaacaga cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag
     1501 cagtgggttc cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga
     1561 cccgtttatg tatcgagcta ggcacttaaa tacaatatct ctgcaatgcg gcaattcagt
     1621 ggttcgtcca atccatgtca gacccgtctg ttgccttcct aataaggcac gatcgtacca
     1681 ccttacttcc accaatcggc atgcacggtg ctttttctct ccttgtaagg catgttgcta
     1741 actcatcgtt accatgttgc aagactacaa gagtattgca taagactaca cgcgtcagct
     1801 tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     1861 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     1921 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc
     1981 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
     2041 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     2101 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     2161 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     2221 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     2281 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     2341 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     2401 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     2461 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     2521 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     2581 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     2641 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     2701 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     2761 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     2821 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     2881 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     2941 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     3001 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     3061 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     3121 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     3181 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     3241 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     3301 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     3361 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     3421 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3481 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3541 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     3601 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3661 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3721 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     3781 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     3841 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     3901 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     3961 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4021 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     4081 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     4141 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     4201 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     4261 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     4321 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     4381 ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg aatttggtct
     4441 ctgcaaaagt ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc
     4501 gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg
     4561 gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggat agctagagcc
     4621 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     4681 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     4741 taaacaagtt cctctcactc tctgatattc atttctttgc aagttataaa tactgaataa
     4801 taagatgaca tgaactacta ctgctagaga ttttccacac tgactaaaag ggtctgaggg
     4861 atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca
     4921 agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga
     4981 tctggtctaa ccagagagac ccagtacaag caaaaagcag atcttatctt cgttgggagt
     5041 gaattagccc ttccagtccc cccttttctt ttaaaaagtg gctaagatct acagctgcct
     5101 tgtaagtcat tggtcttaaa ggtaccaggc ggggaggcgg cccaaaggga gatccgactc
     5161 gtctgagggc gaaggcggag acgcggaaga ggccgcagag ccggcagcag gccgcgggaa
     5221 ggaaggtccg ctggattgag ggccgaaggg acgtagcaga aggacgtccc gcgcagaatc
     5281 caggtggcaa cacaggcgag cagccaagga aaggacgatg atttccccga caacaccacg
     5341 gaattgtcag tgcccaacag ccgagcccct gtccagcagc gggcaaggca ggcggcgatg
     5401 agttccgccg tggcaatagg gagggggaaa gcgaaagtcc cggaaaggag ctgacaggtg
     5461 gtggcaatgc cccaaccagt gggggttgcg tcagcaaaca cagtgcacac cacgccacgt
     5521 tgcctgacaa cgggccacaa ctcctcataa agagacagca accaggattt atacaaggag
     5581 gagaaaatga aagccatacg ggaagcaata gcatgataca aaggcattaa agcagcgtat
     5641 ccacatagcg taaaaggagc aacatagtta agaataccag tcaatctttc acaaattttg
     5701 taatccagag gttgatttca ggcaccgggc ttgcgggtca tgcaccaggt gcgcggtcct
     5761 tcgggcacct cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa ggggaggttg
     5821 cggggcgcgg aggtctccag gaaggcgggc accccggcgc gctcggccgc ctccactccg
     5881 gggagcacga cggcgctgcc cagacccttg ccctggtggt cgggcgagac gccgacggtg
     5941 gccaggaacc acgcgggctc cttgggccgg tgcggcgcca ggaggccttc catctgttgc
     6001 tgcgcggcca gccgggaacc gctcaactcg gccatgcgcg ggccgatctc ggcgaacacc
     6061 gcccccgctt cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc gtcgtccgcg
     6121 acccacacct tgccgatgtc gagcccgacg cgcgtgagga agagttcttg cagctcggtg
     6181 acccgctcga tgtggcggtc cggatcgacg gtgtggcgcg tggcggggta gtcggcgaac
     6241 gcggcggcga gggtgcgtac ggccctgggg acgtcgtcgc gggtggcgag gcgcaccgtg
     6301 ggcttgtact cggtcatagg gccgggattc tcctccacgt caccgcatgt tagaagactt
     6361 cctctgccct cgcgagatcc ggtggagccg ggtccggcgg tgccgtccac ggcagaattg
     6421 gacgactgag cgcgggatct ggcgaaggcg atgggggtct tgaaggcgtg ctggtactcc
     6481 acgatgccca gctcggtgtt gctgtgcagc tcctccacgc ggcggaaggc gaacatgggg
     6541 cccccgttct gcaggatgct ggggtggatg gcgctcttga agtgcatgtg gctgtccacc
     6601 acgaagctgt agtagccgcc gtcgcgcagg ctgaaggtgc gggcgaagct gcccaccagc
     6661 acgttatcgc ccatggggtg caggtgctcc acggtggcgt tgctgcggat gatcttgtcg
     6721 gtgaagatca cgctgtcctc ggggaagccg gtgcccacca ccttgaagtc gccgatcacg
     6781 cggccggcct cgtagcggta gctgaagctc acgtgcagca cgccgccgtc ctcgtacttc
     6841 tcgatgcggg tgttggtgta gccgccgttg ttgatggcgt gcaggaaggg gttctcgtag
     6901 ccgctggggt aggtgccgaa gtggtagaag ccgtagccca tcacgtggct cagcaggtag
     6961 gggctgaagg tcagggcgcc tttggtgctc ttcatcttgt tggtcatgcg gccctgcttg
     7021 ggggtgccct ctccgccgcc caccagctcg aactccacgc cgttcagggt gccggtgatg
     7081 cggcactcga tctccatggc gggcaggccg ctctcgtcgc tctccatggt ggcgtctagc
     7141 gtaggcgccg gtcacagctt ggatctgtaa cggcgcagaa cagaaaacga aacaaagacg
     7201 tagagttgag caagcagggt caggcaaagc gtggagagcc ggctgagtct aggtaggctc
     7261 caagggagcg ccggacaaag gcccggtctc gacctgagct ttaaacttac ctagacggcg
     7321 gacgcagttc aggaggcacc acaggcggga ggcggcagaa cgcgactcaa ccggcgtgga
     7381 tggcggcctc aggtagggcg gcgggcgcgt gaaggagaga tgcgagcccc tcgaagcttc
     7441 agctgtgttc tggcggcaaa cccgttgcga aaaagaacgt tcacggcgac tactgcactt
     7501 atatacggtt ctcccccacc ctcgggaaaa aggcggagcc agtacacgac atcactttcc
     7561 cagtttaccc cgcgccacct tctctaggca cccgttcaat tgccgacccc tccccccaac
     7621 ttctcgggga ctgtgggcga tgtgcgctct gcccactgac gggcaccgga gcgatcgcag
     7681 atccttcgcg gccgcttatt tcccacgacc ttgacgttcc tttttaacac tttcttcttc
     7741 ctcttggcgt ttctcatagt gtgagaagtc atcaaaaatg gaagtggtgt gcttgtagct
     7801 ggctataatt ttcaacacct gcttagcctt ttccagaggc acttcctgag tgtccctaga
     7861 gttggtcact ggtttattct cgttgttctc taggcgaatg tgtcgcagtt ggctattggg
     7921 aacgtccttc acaaaaatcc acctgacatc aaaacgaccc ttccatttgt cctgggaggc
     7981 cacacctgca catgtgttgt agtccacagc agatttcatt tctgccacgc cacagaagtg
     8041 tccactgccg ttgacactga aaagtaagta aacggggcct ttcccgttca tggaacgata
     8101 agcagcatcc agtctcttgt taccatgctc tgtgctgcag gcaatattat acttaatgga
     8161 acggtgaata tcgtcctcag agtagctctt aatgatgaaa acccggccat gtttcagatt
     8221 ccagtcaaaa tctttggggt tatagttatt aatggaccga agcttctcca acactgggtg
     8281 gggttctgaa ggagtagatc cagaaccagc ctgagactgt cctactccat taccatccac
     8341 cccattatga ccgaacccac tgccacggtt ccgaggtgct acccagcggg ttggctgagc
     8401 tgcctgttgc tggactgaaa gctgggcagg ctgtggtgga ggtggaggca atggctgtgt
     8461 ctgttgccct actgatgcct gagccactgg tgggctattg ttagcctgct gacctacagg
     8521 ctgaggagac ccctgggttg gctgacctat attctgaacc aaagcctgtg agggggcttt
     8581 tgcaacggga cccttgttat cccaagttcc aatatccatg ttatgcttta tcgggggtgg
     8641 cggaagactt gaccctgcaa tgccattctt ggtcttcagt ttaggttgct gttttgcagg
     8701 cttgctagca atatcagccc aagatgctgg ttttggagga gcaatggtgg ctggaggcaa
     8761 actattggaa gccacgatgt tactagtaat ggacccgcta ccaacagcag aacctacaac
     8821 ttttggaaca ttgcttgcaa cttctgtgct acccaacttc agtgctgcca tcccttggtc
     8881 tatagtattc atgccaggag ccttattgag ggtctcattg gcaaaagctg actgtccatc
     8941 aatcatggct ccacctaagg agctaggtgc ataagcataa ttgctactat atccagagct
     9001 ctgagtagac tgtccctgag aactgttatt tccccatgct gagaagtcaa tcccactggg
     9061 aaagaaatta aaaccatgct gaccaagaaa tggagtgcta cctagggctc ctggttgccc
     9121 aaacattgca tctggtagga agtggggctc tccgttgctc agctgtccat aagaagttaa
     9181 gtagggcatg gctgtgtcac cccccgtaga ccaagcagct tcacccaaag aataggagaa
     9241 gccaatggag ggactgtagt aactgggtaa gtaggaatct gacatggcag tatatgcatt
     9301 attgggcctt gcctgtggac tcaagtaagg ttcaaaatca tcatcgttta atccatcctt
     9361 ttgatgtaca gatccatttt gtactttgtt tccttgacct tttggtctct gctccaagag
     9421 gctgctggcc gagaattcgc tagccttgtc atcgtcatcc ttgtagtcga tgtcatgatc
     9481 tttataatca ccgtcatggt ctttgtaatc cattctagaa tcttctatgg aggtcaaaac
     9541 agcgtggatg gcgtctccag gcgatctgac ggttcactaa acgagctctg cttatataaa
     9601 cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt
     9661 ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga
     9721 gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc
     9781 gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc
     9841 ataaggtcat gtactgggca taatactagt atcgataaaa ttttgaattt tgtaatttgt
     9901 ttttgtaatt ctttagtttg tatgtctgtt gctattatgt ctactattct ttcccctgca
     9961 ctgtaccccc caatcccccc ttttctttta aaagttaacc gata
//
