LOCUS       sequence-474761-       10004 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the domain-mutant YTHDF2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10004)
  AUTHORS   Chen Z, Zeng C, Yang L, Che Y, Chen M, Sau L, Wang B, Zhou K, Chen 
            Y, Qing Y, Shen C, Zhang T, Wunderlich M, Wu D, Li W, Wang K, Leung 
            K, Sun M, Tang T, He X, Zhang L, Swaminathan S, Mulloy JC, Muschen 
            M, Huang H, Weng H, Xiao G, Deng X, Chen J
  TITLE     YTHDF2 promotes ATP synthesis and immune evasion in B cell 
            malignancies.
  JOURNAL   Cell. 2024 Dec 12:S0092-8674(24)01324-2. doi: 
            10.1016/j.cell.2024.11.007.
  PUBMED    39694037
REFERENCE   2  (bases 1 to 10004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Dec 12:S0092-8674(24)01324-2. doi: 10.1016/j.cell.2024.11.007."
COMMENT     Sequence Label: pCDH-3_Flag-YTHDF2-D-MUT
FEATURES             Location/Qualifiers
     source          1..10004
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             12..65
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             66..665
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(66..85)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     562..582
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    666..1254
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(719..739)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1137..1148)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1328..1561
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1633..1754
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1670..1689)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1724..1743
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1794..1929
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1962..1978)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1962..1978)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1975..1997)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1986..2002
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2010..2016,2017..2034,2035..2040))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2055..2076
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2193..2210)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2364..2952)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(3123..3914,3915..3983))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3746..3765
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3984..4088)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4156..4174
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4212..4234)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4334..4353
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4547..4569
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4561..4578
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4562..4578
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4593..4819
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4820..5000
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5047..5172
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5665..5898
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6083..6127
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6394..6510
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6600..6803
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6754..6774
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6800..6824
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6877..6942
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        8733..8944
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             8957..9225
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      9254..9263
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9284..9946
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
ORIGIN
        1 ccggatctcg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg
       61 gccctatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
      121 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
      181 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
      241 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
      301 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
      361 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
      421 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
      481 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
      541 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
      601 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
      661 cctgaaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
      721 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
      781 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
      841 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
      901 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
      961 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     1021 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     1081 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     1141 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     1201 gtctccgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggta
     1261 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     1321 gggggactgg aagggctaat tcactcccaa cgaagataag atctgctttt tgcttgtact
     1381 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     1441 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     1501 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     1561 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     1621 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     1681 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     1741 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc
     1801 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     1861 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     1921 ttggaggcct agacttttgc agagaccaaa ttcgtaatca tgtcatagct gtttcctgtg
     1981 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     2041 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     2101 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     2161 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     2221 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     2281 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2341 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2401 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2461 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2521 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2581 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2641 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2701 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2761 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2821 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2881 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2941 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     3001 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     3061 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     3121 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     3181 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     3241 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     3301 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     3361 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     3421 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     3481 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     3541 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     3601 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     3661 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     3721 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     3781 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     3841 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     3901 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     3961 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     4021 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     4081 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     4141 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
     4201 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     4261 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     4321 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
     4381 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg
     4441 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     4501 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     4561 tgtaaaacga cggccagtgc caagctgacg cgtgtagtct tatgcaatac tcttgtagtc
     4621 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
     4681 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
     4741 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
     4801 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
     4861 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     4921 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     4981 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     5041 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     5101 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     5161 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     5221 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     5281 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     5341 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     5401 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     5461 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccactgat
     5521 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     5581 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     5641 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     5701 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     5761 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     5821 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     5881 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     5941 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     6001 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     6061 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     6121 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     6181 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     6241 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     6301 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     6361 attagtgaac ggatctcgac ggtatcggtt aacttttaaa agaaaagggg ggattggggg
     6421 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     6481 acaaaaacaa attacaaaat tcaaaatttt atcgatacta gtattatgcc cagtacatga
     6541 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     6601 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     6661 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     6721 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     6781 gggaggttta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc
     6841 cacgctgttt tgacctccat agaagattct agaatggatt acaaagacca tgacggtgat
     6901 tataaagatc atgacatcga ctacaaggat gacgatgaca aggctagcga attctcggcc
     6961 agcagcctct tggagcagag accaaaaggt caaggaaaca aagtacaaaa tggatctgta
     7021 catcaaaagg atggattaaa cgatgatgat tttgaacctt acttgagtcc acaggcaagg
     7081 cccaataatg catatactgc catgtcagat tcctacttac ccagttacta cagtccctcc
     7141 attggcttct cctattcttt gggtgaagct gcttggtcta cggggggtga cacagccatg
     7201 ccctacttaa cttcttatgg acagctgagc aacggagagc cccacttcct accagatgca
     7261 atgtttgggc aaccaggagc cctaggtagc actccatttc ttggtcagca tggttttaat
     7321 ttctttccca gtgggattga cttctcagca tggggaaata acagttctca gggacagtct
     7381 actcagagct ctggatatag tagcaattat gcttatgcac ctagctcctt aggtggagcc
     7441 atgattgatg gacagtcagc ttttgccaat gagaccctca ataaggctcc tggcatgaat
     7501 actatagacc aagggatggc agcactgaag ttgggtagca cagaagttgc aagcaatgtt
     7561 ccaaaagttg taggttctgc tgttggtagc gggtccatta ctagtaacat cgtggcttcc
     7621 aatagtttgc ctccagccac cattgctcct ccaaaaccag catcttgggc tgatattgct
     7681 agcaagcctg caaaacagca acctaaactg aagaccaaga atggcattgc agggtcaagt
     7741 cttccgccac ccccgataaa gcataacatg gatattggaa cttgggataa caagggtccc
     7801 gttgcaaaag ccccctcaca ggctttggtt cagaatatag gtcagccaac ccaggggtct
     7861 cctcagcctg taggtcagca ggctaacaat agcccaccag tggctcaggc atcagtaggg
     7921 caacagacac agccattgcc tccacctcca ccacagcctg cccagctttc agtccagcaa
     7981 caggcagctc agccaacccg ctgggtagca cctcggaacc gtggcagtgg gttcggtcat
     8041 aatggggtgg atggtaatgg agtaggacag tctcaggctg gttctggatc tactccttca
     8101 gaaccccacc cagtgttgga gaagcttcgg tccattaata actataaccc caaagatttt
     8161 gactggaatc tgaaacatgg ccgggttttc atcattaaga gcgcctctga ggacgccatt
     8221 caccgttcca ttaagtataa tattgcctgc agcacagagc atggtaacaa gagactggat
     8281 gctgcttatc gttccatgaa cgggaaaggc cccgtttact tacttttcag tgtcaacggc
     8341 agtggacact tctgtggcgt ggcagaaatg aaatctgctg tggactacaa cacatgtgca
     8401 ggtgtggcct cccaggacaa agccaagggt cgttttgatg tcaggtggat ttttgtgaag
     8461 gacgttccca atagccaact gcgacacatt cgcctagaga acaacgagaa taaaccagtg
     8521 accaactcta gggacactca ggaagtgcct ctggaaaagg ctaagcaggt gttgaaaatt
     8581 atagccagct acaagcacac cacttccatt tttgatgact tctcacacta tgagaaacgc
     8641 caagaggaag aagaaagtgt taaaaaggaa cgtcaaggtc gtgggaaata agcggccgcg
     8701 aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     8761 cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt
     8821 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     8881 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     8941 acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg
     9001 ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac
     9061 tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct
     9121 cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca
     9181 actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc
     9241 gcctacgcta gacgccacca tggagagcga cgagagcggc ctgcccgcca tggagatcga
     9301 gtgccgcatc accggcaccc tgaacggcgt ggagttcgag ctggtgggcg gcggagaggg
     9361 cacccccaag cagggccgca tgaccaacaa gatgaagagc accaaaggcg ccctgacctt
     9421 cagcccctac ctgctgagcc acgtgatggg ctacggcttc taccacttcg gcacctaccc
     9481 cagcggctac gagaacccct tcctgcacgc catcaacaac ggcggctaca ccaacacccg
     9541 catcgagaag tacgaggacg gcggcgtgct gcacgtgagc ttcagctacc gctacgaggc
     9601 cggccgcgtg atcggcgact tcaaggtggt gggcaccggc ttccccgagg acagcgtgat
     9661 cttcaccgac aagatcatcc gcagcaacgc caccgtggag cacctgcacc ccatgggcga
     9721 taacgtgctg gtgggcagct tcgcccgcac cttcagcctg cgcgacggcg gctactacag
     9781 cttcgtggtg gacagccaca tgcacttcaa gagcgccatc caccccagca tcctgcagaa
     9841 cgggggcccc atgttcgcct tccgccgcgt ggaggagctg cacagcaaca ccgagctggg
     9901 catcgtggag taccagcacg ccttcaagac ccccatcgcc ttcgccagat cccgcgctca
     9961 gtcgtccaat tctgccgtgg acggcaccgc cggacccggc tcca
//
