LOCUS       sequence-47495-e         480 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ChETA-EYFP in cells lacking Cre (Cre-Off), for optogenetic
            excitation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Saunders A, Johnson CA, Sabatini BL
  TITLE     Novel recombinant adeno-associated viruses for Cre activated and 
            inactivated transgene expression in neurons.
  JOURNAL   Front Neural Circuits. 2012 Jul 27;6:47. doi: 
            10.3389/fncir.2012.00047. eCollection 2012.
  PUBMED    22866029
REFERENCE   2  (bases 1 to 480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Neural Circuits."; date: "2012-07-27"; volume: "6:47. doi"; pages: "
            10.3389/fncir.2012.00047. eCollection 2012"
COMMENT     Sequence Label: pAAV-Ef1a-DO-ChETA-EYFP-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..480
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(12..45)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      67..76
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gaaacctctt aataacttcg tatagcatac attatacgaa gttatggcgc gccttatcta
       61 gatttagcca ccatggacta tggcggcgct ttgtctgccg tcggacgcga acttttgttc
      121 gttactaatc ctgtggtggt gaacgggtcc gtcctggtcc ctgaggatca atgttactgt
      181 gccggatgga ttgaatctcg cggcacgaac ggcgctcaga ccgcgtcaaa tgtcctgcag
      241 tggcttgcag caggattcag cattttgctg ctgatgttct atgcctacca aacctggaaa
      301 tctacatgcg gctgggagga gatctatgtg tgcgccattg aaatggttaa ggtgattctc
      361 gagttctttt ttgagtttaa gaatccctct atgctctacc ttgccacagg acaccgggtg
      421 cagtggctgc gctatgcaac ctggctgctc acttgtcctg tcatccttat ccgcctgagc
//
