LOCUS       sequence-474953-        6047 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express HiBiT-SHOC2 (N-terminal) in Mammalian Cells under a CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6047)
  AUTHORS   Promega
  TITLE     Promega Plasmid Collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: HiBiT-SHOC2 Fusion Vector
FEATURES             Location/Qualifiers
     source          1..6047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1033..1052
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2990..3008)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    3045..3166
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3056..3075)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3110..3129
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        3411..3768
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3411..3602
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3434..3454)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3619..3754
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        3783..3830
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3862..4656
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3916..3935)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4526..4545
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4720..4768
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(5007..5595)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5087..5106)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    5711..5994
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgct caccatggtg agcggctggc
     1081 ggctgttcaa gaagattagc gggagctccg gtggctcgag cggtgcgatc gccatgagta
     1141 gtagtttagg aaaagaaaaa gactctaaag aaaaagatcc caaagtacca tcagccaagg
     1201 aaagagaaaa ggaggcaaaa gcctctggag gttttgggaa agagagcaaa gaaaaagaac
     1261 ctaagaccaa agggaaagat gccaaagatg gaaagaagga ctccagtgct gcccaaccag
     1321 gggtggcatt ttcagttgac aatacgatca aacggccaaa cccagcacct gggactagaa
     1381 aaaaatccag caatgcagag gtgattaaag agctcaacaa atgccgggaa gagaattcaa
     1441 tgcgtttgga cttatccaag agatctatac acatattgcc atcatcaatc aaagagttga
     1501 ctcaattaac agaactttat ttatacagta acaaattgca gtccctccca gcagaggtgg
     1561 gatgtttagt aaatctcatg acactggctc taagtgaaaa ttcacttacc agtttgcctg
     1621 actctcttga taacttgaag aagctgcgga tgcttgattt acggcataat aaactgagag
     1681 aaattccttc agtggtgtat aggctggatt ctctcaccac tctttacctt cgctttaatc
     1741 gtataactac tgtggaaaag gacatcaaaa acttgtcaaa actcagcatg cttagcattc
     1801 gagagaacaa aattaaacaa ctacctgctg aaattggtga attatgtaac ctcattacgc
     1861 tggatgtagc tcacaatcaa cttgaacacc ttccaaagga gattggaaac tgtacacaga
     1921 taaccaacct tgacttgcag cacaatgaac tgctagacct cccagatact ataggaaacc
     1981 tgtccagttt aagtcgtctt ggtctgagat ataacagact gtcagcaata cccagatcat
     2041 tagcaaaatg cagtgcactt gaagaattaa atttagagaa caataacatt tctactttac
     2101 cagagagtct tttatcaagt cttgtgaaac tgaatagttt gaccttagct agaaattgct
     2161 tccagttgta tccagtgggt ggtccatctc agttttctac catctattcc ctcaacatgg
     2221 aacacaatcg aatcaacaaa attccatttg gaattttctc cagagcaaaa gtattaagta
     2281 agctgaatat gaaggacaat cagttaacat cacttccctt ggattttgga acttggacca
     2341 gtatggtaga attgaattta gccactaatc agctcacaaa gatccctgag gatgtgtctg
     2401 gtctcgtttc tcttgaggtt cttatcttat caaacaatct tctaaagaag cttccccatg
     2461 gtcttggaaa ccttaggaag ttaagagagt tggatctaga agagaacaaa ttggaatcct
     2521 tgccaaatga aattgcatat cttaaggatt tacagaaatt agtcttgaca aacaaccagt
     2581 tgaccactct tcccagaggc attggtcacc ttactaatct cacacatctg ggccttggag
     2641 agaacctact tactcacctt cctgaagaaa ttggtacact ggagaaccta gaagaactgt
     2701 atttgaatga caaccccaac ctgcatagcc ttccctttga gctggcactc tgcagcaagc
     2761 tttcaatcat gagtattgag aactgtccac tcagtcacct tccacctcag attgttgctg
     2821 gggggccttc tttcatcatt cagttcttaa agatgcaggg tccatatcgt gccatggttt
     2881 aaacgaattc gggctcggta cccggggatc ctctagagtc gacctgcagg catgcaagct
     2941 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
     3001 taactagcat aaccccttgg ggcggccgct tcgagcagac atgataagat acattgatga
     3061 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     3121 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     3181 cattcatttt atgtttcagg ttcaggggga gatgtgggag gtttttttaa gcaagtaaaa
     3241 cctctacaaa tgtggtaaaa tcgaatttta acaaaatatt aacgcttaca atttcctgat
     3301 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgcg gatctgcgca
     3361 gcaccatggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
     3421 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     3481 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     3541 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     3601 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     3661 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     3721 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttaattaa
     3781 ctgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
     3841 actaaaccca ggaggcagat catgattgaa caagatggat tgcacgcagg ttctccggcc
     3901 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     3961 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     4021 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     4081 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     4141 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     4201 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     4261 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     4321 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     4381 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     4441 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     4501 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     4561 ggcgaatggg ctgaccggtt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     4621 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     4681 ccgaccaagc gacgcccaac ctgccatcac gatggccgca ataaaatatc tttattttca
     4741 ttacatctgt gtgttggttt tttgtgtgaa tcgatagcga taaggatcct ctttgcgctt
     4801 gcgttttccc ttgtccagat agcccagtag ctgacattca tccggggtca gcaccgtttc
     4861 tgcggactgg ctttctaccc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     4921 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     4981 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     5041 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     5101 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     5161 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     5221 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     5281 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     5341 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     5401 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     5461 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     5521 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     5581 aaaaaaggat ttcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     5641 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     5701 cttttatagt ccggaaatac aggaacgcac gctggatggc ccttcgctgg gatggtgaaa
     5761 ccatgaaaaa tggcagcttc agtggattaa gtgggggtaa tgtggcctgt accctctggt
     5821 tgcataggta ttcatacggt taaaatttat caggcgcgat tgcggcagtt tttcgggtgg
     5881 tttgttgcca tttttacctg tctgctgccg tgatcgcgct gaacgcgttt tagcggtgcg
     5941 tacaattaag ggattatggt aaatccactt actgtctgcc ctcgtagcca tcgagataaa
     6001 ccgcagtact ccggccacga tgcgtccggc gtagaggatc gagatct
//
