LOCUS       sequence-47497-e       12124 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12124)
  AUTHORS   Tuttle JR, Haigler CH, Robertson D
  TITLE     Method: low-cost delivery of the cotton leaf crumple virus-induced 
            gene silencing system.
  JOURNAL   Plant Methods. 2012 Aug 1;8(1):27. doi: 10.1186/1746-4811-8-27.
  PUBMED    22853641
REFERENCE   2  (bases 1 to 12124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2012-08-1"; volume: "8(1):27. doi"; pages: " 
            10.1186/1746-4811-8-27"
COMMENT     Sequence Label: pJRT.Agro.CLCrVA.010
FEATURES             Location/Qualifiers
     source          1..12124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(465..493)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1088..1108
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(3226..3243)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3226..3242)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3235..3257)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    3445..3469
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4769..5398
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5832..6899
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      6965..7159
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7395..7417)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7503..7643
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7558..7577
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7658..7675)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7829..8417)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7909..7928)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8504..9298)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9723..9747
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    9825..9999
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(10039..11064)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(11132..11809)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    12000..12021
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12036..12041,12042..12059,12060..12066)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12074..12090
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12079..12101
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12098..12114
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12098..12114
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aattcgagct ccacttggga taggttaaga aaatatgtct ggcttgtaaa cgaaaagaat
       61 cggggtttct aggcattttt taaatagtga cccaggactc aaaaactagc tctcaacttc
      121 tctcatatgt tgtggagtct ggagtccttt atatatgttt acgctataga aggcatttgg
      181 ggactctccc gggcccacca taggactcca taccgcggcc atctatataa tattactgat
      241 ggccgcgcgg attttggagc ccccttttgt cgttagcgcg acctttaatt caaattaaag
      301 gttgcttttt tctatcgtcc aatcattttg cgcctgacta gcctagatat tagcaacaac
      361 ttcgcgacta agttgttgac cgtcaagtat aaattaatgg gcttatggcc taatgggtat
      421 agagcaaaat ggcatgcagt aaaggagaag aacttttcac tggagttgtc ccaattcttg
      481 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
      541 atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc
      601 catggccaac acttgtcact actttcactt atggtgttca atgcttttca agatacccag
      661 atcatatgaa gcggcacgac ttcttcaaga gcgccatgcc tgagggatac gtgcaggaga
      721 ggaccatctc tttcaaggac gacgggaact acaagacacg tgctgaagtc aagtttgagg
      781 gagacaccct cgtcaacagg atcgagctta agggaatcga tttcaaggag gacggaaaca
      841 tcctcggcca caagttggaa tacaactaca actcccacaa cgtatacatc acggcagaca
      901 aacaaaagaa tggaatcaaa gctaacttca aaattagaca caacattgaa gatggaagcg
      961 ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac
     1021 cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag
     1081 accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac
     1141 tatacaaaga tccataatcc tgtgtatgca acgttgaaaa ttcggatcta tttttacgat
     1201 tcgatcatga attaataaaa tttaaatttt attgaatgat tttctagtac ataactgaca
     1261 tacgacttat cagttgcgaa acgaacagct ctaattacat tgttaagtga aattacgcct
     1321 aattcgtcta agtacaacat aaccaactgt ttaaacctaa ttaaataagt cgacccagaa
     1381 gctgtcagag atgtcgtcca gacttggaag ttcaggtaag ctttgtggag atgcaacgct
     1441 ctccgtaagt tgtggttgaa tcggatctgg acgtggtata tacgcgtcct ggtgtatagc
     1501 agatcctcta ctccgtatat cttgaaatat aggggattta ttatctccca gatatacacg
     1561 ccattctctg cctgacgtgc agtgatgatt tcccctgtgc gtgaatccat gaccggtgca
     1621 gcctatgtgg aagtatatgg tgcacccaca gtttagatca atgcgtcgtc gtctaatcgc
     1681 cctcctcttg gcttgcctgt gtgccttctt gatagagggg ggcgtcgaga gtgatgaaga
     1741 taacattctt gagagtccaa tgtttgagcg acgcattttc cgctttatcc aggaaatctt
     1801 tatagctgga tccctcgcct ggattgcaaa gcacgattga agggatccct cctttaattt
     1861 gaactggttt cccgtacttg caatttgact gccagtcttt ttgggcccca agaagctctt
     1921 tccaatgctt tagctttagg tattgcggtg ctacgtcatc aatgacgtta taggcaacat
     1981 tattcgagta cactcgatta ttgaagtcca ggtgtccact gaggtaatta tgtgggccta
     2041 aagcacgagc ccacatcgtc ttgcccgttc tagaatcacc ttccactatg agactaactg
     2101 gtcttacagg ccgcgcagcg ggacccattc caaaataatc atccgcccat tcttgcatct
     2161 gttcaggaac gttagtgaaa gaggaaagtg gaaatggagg gacccattgt tcaggcgcct
     2221 ttttgaaaag gcgttccaag ttcgctgtaa tgttatggta actcacgatg aacgcccttg
     2281 gatctccggc ccggataatt gcaagagctt ccgccaaact aggtgcattg atggcgttat
     2341 aatagacgtc gtctttattc ttctttgtac ccccaggcac tttgaactgt ccggattcac
     2401 aacaatcgcc gtctttggtg atgtaattct tgacggcgtt ggcgtctttg gctgcttgta
     2461 tgtttgggtg aaaattggtc gaccttctgg ggtgagtaag gtcgaaaaat ctcttattct
     2521 tgatgttgga ctttccagag agttggatga gacagtgaag gtgtgggaat ccgtccgagt
     2581 gctcctctct ggcaactcga atatatgttg gtttgacgac tgaccatgat aaggtttgaa
     2641 gcatttgaag agcttcattt tttgggatat cgcacttggg ataggttaag aaaatatgtc
     2701 tggcttgtaa acgaaaagaa tcggggtttc taggcatttt ttaaatagtg acccaggact
     2761 caaaaactag ctctcaactt ctctcatatg ttgtggagtc tggagtcctt tatatatgtt
     2821 tacgctatag aaggcatttg gggactctcc cgggcccacc ataggactcc ataccgcggc
     2881 catctatata atattactga tggccgcgcg gattttggag cccccttttg tcgttagcgc
     2941 gacctttaat tcaaattaaa ggttgctttt ttctatcgtc cgatcatttt gcgcctgact
     3001 agcctagata ttagcaacaa cttcgcggct aagttgttga ccgtcaagta taaattaatg
     3061 ggcttatggc ccaatgggta tagagcaaaa tgcccaagcg tgatgccccg tggcgcttat
     3121 tagcgggtac gtctaaggta agtcgaaatg ccaactactc tcccttgaga gtattgggcc
     3181 ctaaagttaa taagggacct cgacctgcag gcatgcaagc ttggcactgg ccgtcgtttt
     3241 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     3301 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     3361 gcgcagcctg aatggcgaat gctagagcag cttgagcttg gatcagattg tcgtttcccg
     3421 ccttcagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa
     3481 gagcgtttat tagaataacg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca
     3541 tttgtatgtg catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc
     3601 ctccgctgct atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac
     3661 atgtcgcaca agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt
     3721 cttgtcgcgt gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac
     3781 gccatgaaca agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac
     3841 caggacttga ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc
     3901 gagaagatca ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta
     3961 cgccctggcg acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac
     4021 ctactggaca ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag
     4081 ccgtgggccg acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt
     4141 gccgagttcg agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag
     4201 gcccgaggcg tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc
     4261 cgcgagctga tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg
     4321 catcgctcga ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc
     4381 aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc
     4441 gagaatgaac gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt
     4501 ttttcattac cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc
     4561 ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc
     4621 tggcggcctg gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt
     4681 gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag
     4741 taaataaaca aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg
     4801 cgggtcaggc aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc
     4861 cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg
     4921 ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa
     4981 ggccatcggc cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc
     5041 tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga
     5101 catatgggcc accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg
     5161 aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga
     5221 ggttgccgag gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg
     5281 cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg
     5341 cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt
     5401 taatgaggta aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc
     5461 gcacgcagca gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg
     5521 gtcaactttc agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa
     5581 ggcaagacca ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc
     5641 aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga
     5701 acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg
     5761 cgtaagcggc tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga
     5821 atcggcgtga cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg
     5881 acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag
     5941 cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac
     6001 cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt
     6061 ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg
     6121 ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc
     6181 cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg
     6241 acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga
     6301 agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc
     6361 ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca
     6421 ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat
     6481 ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg
     6541 agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc
     6601 cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc
     6661 tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga
     6721 tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc
     6781 tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc
     6841 cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat
     6901 gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct
     6961 ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt
     7021 acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa
     7081 aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa
     7141 cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc
     7201 ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg
     7261 ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg
     7321 cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt
     7381 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     7441 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     7501 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
     7561 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     7621 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     7681 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     7741 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7801 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7861 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7921 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7981 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     8041 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     8101 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     8161 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     8221 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     8281 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     8341 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     8401 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     8461 acgaaaactc acgttaaggg attttggtca tgcattctag gtactaaaac aattcatcca
     8521 gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata
     8581 gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt
     8641 cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat
     8701 ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg
     8761 gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt
     8821 cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta
     8881 agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc
     8941 tgatgcactc cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt
     9001 ccgagcaaag gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt
     9061 caaagtgcag gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt
     9121 cccgttccac atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt
     9181 tttcattttc tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta
     9241 cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt ttagccattt
     9301 attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa
     9361 gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt
     9421 ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc
     9481 gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga
     9541 gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac
     9601 atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg
     9661 ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct
     9721 ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg
     9781 cggacgtttt taatgtactg aattaacgcc gaattaattc gggggatctg gattttagta
     9841 ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat
     9901 acatactaag ggtttcttat atgctcaaca catgagcgaa accctatagg aaccctaatt
     9961 cccttatctg ggaactactc acacattatt atggagaaac tcgagcttgt cgatcgacag
    10021 atccggtcgg catctactct atttctttgc cctcggacga gtgctggggc gtcggtttcc
    10081 actatcggcg agtacttcta cacagccatc ggtccagacg gccgcgcttc tgcgggcgat
    10141 ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc
    10201 ccaagctgca tcatcgaaat tgccgtcaac caagctctga tagagttggt caagaccaat
    10261 gcggagcata tacgcccgga gtcgtggcga tcctgcaagc tccggatgcc tccgctcgaa
    10321 gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc cagaagaaga tgttggcgac
    10381 ctcgtattgg gaatccccga acatcgcctc gctccagtca atgaccgctg ttatgcggcc
    10441 attgtccgtc aggacattgt tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg
    10501 gcagtcctcg gcccaaagca tcagctcatc gagagcctgc gcgacggacg cactgacggt
    10561 gtcgtccatc acagtttgcc agtgatacac atggggatca gcaatcgcgc atatgaaatc
    10621 acgccatgta gtgtattgac cgattccttg cggtccgaat gggccgaacc cgctcgtctg
    10681 gctaagatcg gccgcagcga tcgcatccat agcctccgcg accggttgta gaacagcggg
    10741 cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt gcacggcggg agatgcaata
    10801 ggtcaggctc tcgctaaact ccccaatgtc aagcacttcc ggaatcggga gcgcggccga
    10861 tgcaaagtgc cgataaacat aacgatcttt gtagaaacca tcggcgcagc tatttacccg
    10921 caggacatat ccacgccctc ctacatcgaa gctgaaagca cgagattctt cgccctccga
    10981 gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc agaaacttct cgacagacgt
    11041 cgcggtgagt tcaggctttt tcatatctca ttgccccccg ggatctgcga aagctcgaga
    11101 gagatagatt tgtagagaga gactggtgat ttcagcgtgt cctctccaaa tgaaatgaac
    11161 ttccttatat agaggaaggt cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc
    11221 agtggagata tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc
    11281 cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg
    11341 aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac
    11401 tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat
    11461 taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt
    11521 cttggagtag acgagagtgt cgtgctccac catgttatca catcaatcca cttgctttga
    11581 agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt
    11641 gggaccactg tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca
    11701 tttgtaggtg ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca
    11761 atggaatccg aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg
    11821 gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat
    11881 gttggcaagc tgctctagcc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
    11941 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
    12001 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
    12061 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
    12121 tacg
//
