LOCUS       sequence-47498-e       11950 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11950)
  AUTHORS   Tuttle JR, Haigler CH, Robertson D
  TITLE     Method: low-cost delivery of the cotton leaf crumple virus-induced 
            gene silencing system.
  JOURNAL   Plant Methods. 2012 Aug 1;8(1):27. doi: 10.1186/1746-4811-8-27.
  PUBMED    22853641
REFERENCE   2  (bases 1 to 11950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2012-08-1"; volume: "8(1):27. doi"; pages: " 
            10.1186/1746-4811-8-27"
COMMENT     Sequence Label: pJRT.Agro.CLCrVA.009
FEATURES             Location/Qualifiers
     source          1..11950
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3052..3069)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3052..3068)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3061..3083)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    3271..3295
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4595..5224
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5658..6725
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      6791..6985
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7221..7243)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7329..7469
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7384..7403
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7484..7501)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7655..8243)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7735..7754)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8330..9124)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9549..9573
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    9651..9825
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9865..10890)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(10958..11635)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    11826..11847
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11862..11867,11868..11885,11886..11892)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11900..11916
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11905..11927
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11924..11940
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11924..11940
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aattcgagct ccacttggga taggttaaga aaatatgtct ggcttgtaaa cgaaaagaat
       61 cggggtttct aggcattttt taaatagtga cccaggactc aaaaactagc tctcaacttc
      121 tctcatatgt tgtggagtct ggagtccttt atatatgttt acgctataga aggcatttgg
      181 ggactctccc gggcccacca taggactcca taccgcggcc atctatataa tattactgat
      241 ggccgcgcgg attttggagc ccccttttgt cgttagcgcg acctttaatt caaattaaag
      301 gttgcttttt tctatcgtcc gatcattttg cgcctgacta gcctagatat tagcaacaac
      361 ttcgcggcta agttgttgac cgtcaagtat aaattaatgg gcttatggcc taatgggtat
      421 agagcaaaat ggcatgcctg cagactagtg aattcgctag cgttaactgg ccaatcaaac
      481 cgtgctcttt cctccacaat cttcactcta agctcagcgt ccctcactgt cccgacttga
      541 gcatgcattc cgaatcgatc aagaagctgt ggtctaagct ctccttcttc tggattacct
      601 gagccgatga gaataaaccg agcaggatgt gagatcgaaa taccttccct ctcaacagtg
      661 ttccatcctg atgcagcgga atccaaaaga acatccacca aatggtcatc taaaaggtta
      721 acttcatcga cgtaaagaat ccctcgatta gctttagcta gaagtccagg ctcaaatgcc
      781 ttgacaccct cagtgagggc tttctcaatg tcaatggttc cacagaccct atcttcagta
      841 gcacctaatg gcaaatccac catgttgatt ttagtcattg taatagtcag ttcctctcca
      901 tttacaacct tttctctaac ttctatgccc attgattccg gatcctccgg gtccgagtta
      961 taagggtcac cgaatggcca taatcctgtg tatgcaacgt tgaaaattcg gatctatttt
     1021 tacgattcga tcatgaatta ataaaattta aattttattg aatgattttc tagtacataa
     1081 ctgacatacg acttatcagt tgcgaaacga acagctctaa ttacattgtt aagtgaaatt
     1141 acgcctaatt cgtctaagta caacataacc aactgtttaa acctaattaa ataagtcgac
     1201 ccagaagctg tcagagatgt cgtccagact tggaagttca ggtaagcttt gtggagatgc
     1261 aacgctctcc gtaagttgtg gttgaatcgg atctggacgt ggtatatacg cgtcctggtg
     1321 tatagcagat cctctactcc gtatatcttg aaatataggg gatttattat ctcccagata
     1381 tacacgccat tctctgcctg acgtgcagtg atgatttccc ctgtgcgtga atccatgacc
     1441 ggtgcagcct atgtggaagt atatggtgca cccacagttt agatcaatgc gtcgtcgtct
     1501 aatcgccctc ctcttggctt gcctgtgtgc cttcttgata gaggggggcg tcgagagtga
     1561 tgaagataac attcttgaga gtccaatgtt tgagcgacgc attttccgct ttatccagga
     1621 aatctttata gctggatccc tcgcctggat tgcaaagcac gattgaaggg atccctcctt
     1681 taatttgaac tggtttcccg tacttgcaat ttgactgcca gtctttttgg gccccaagaa
     1741 gctctttcca atgctttagc tttaggtatt gcggtgctac gtcatcaatg acgttatagg
     1801 caacattatt cgagtacact cgattattga agtccaggtg tccactgagg taattatgtg
     1861 ggcctaaagc acgagcccac atcgtcttgc ccgttctaga atcaccttcc actatgagac
     1921 taactggtct tacaggccgc gcagcgggac ccattccaaa ataatcatcc gcccattctt
     1981 gcatctgttc aggaacgtta gtgaaagagg aaagtggaaa tggagggacc cattgttcag
     2041 gcgccttttt gaaaaggcgt tccaagttcg ctgtaatgtt atggtaactc acgatgaacg
     2101 cccttggatc tccggcccgg ataattgcaa gagcttccgc caaactaggt gcattgatgg
     2161 cgttataata gacgtcgtct ttattcttct ttgtaccccc aggcactttg aactgtccgg
     2221 attcacaaca atcgccgtct ttggtgatgt aattcttgac ggcgttggcg tctttggctg
     2281 cttgtatgtt tgggtgaaaa ttggtcgacc ttctggggtg agtaaggtcg aaaaatctct
     2341 tattcttgat gttggacttt ccagagagtt ggatgagaca gtgaaggtgt gggaatccgt
     2401 ccgagtgctc ctctctggca actcgaatat atgttggttt gacgactgac catgataagg
     2461 tttgaagcat ttgaagagct tcattttttg ggatatcgca cttgggatag gttaagaaaa
     2521 tatgtctggc ttgtaaacga aaagaatcgg ggtttctagg cattttttaa atagtgaccc
     2581 aggactcaaa aactagctct caacttctct catatgttgt ggagtctgga gtcctttata
     2641 tatgtttacg ctatagaagg catttgggga ctctcccggg cccaccatag gactccatac
     2701 cgcggccatc tatataatat tactgatggc cgcgcggatt ttggagcccc cttttgtcgt
     2761 tagcgcgacc tttaattcaa attaaaggtt gcttttttct atcgtccaat cattttgcgc
     2821 ctgactagcc tagatattag caacaacttc gcgactaagt tgttgaccgt caagtataaa
     2881 ttaatgggct tatggcccaa tgggtataga gcaaaatgcc caagcgtgat gccccgtggc
     2941 gcttattagc gggtacgtct aaggtaagtc gaaatgccaa ctactctccc ttgagagtat
     3001 tgggccctaa agttaataag ggacctcgac ctgcaggcat gcaagcttgg cactggccgt
     3061 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     3121 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     3181 acagttgcgc agcctgaatg gcgaatgcta gagcagcttg agcttggatc agattgtcgt
     3241 ttcccgcctt cagtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag
     3301 agaaaagagc gtttattaga ataacggata tttaaaaggg cgtgaaaagg tttatccgtt
     3361 cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc
     3421 caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa
     3481 aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg
     3541 cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga
     3601 cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc
     3661 gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg
     3721 ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac
     3781 cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc
     3841 cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg
     3901 gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc
     3961 ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc
     4021 gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg
     4081 cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt
     4141 ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc
     4201 gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg
     4261 gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg
     4321 aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg
     4381 agccgcccgc gcacgtctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg
     4441 ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa
     4501 aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc
     4561 gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca
     4621 gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc
     4681 cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc
     4741 cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga
     4801 cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga
     4861 cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc
     4921 ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac
     4981 ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg
     5041 cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac
     5101 gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc
     5161 cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat
     5221 ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt
     5281 ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg
     5341 aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta
     5401 cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa
     5461 atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa
     5521 tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg
     5581 gccaggcgta agcggctggg ttgtctgccg gccctgcaat ggcactggaa cccccaagcc
     5641 cgaggaatcg gcgtgacggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg
     5701 gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg
     5761 cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc
     5821 ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac
     5881 cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg
     5941 acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg
     6001 agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg
     6061 attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg
     6121 aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt
     6181 tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt
     6241 taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga
     6301 cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc
     6361 ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca
     6421 agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc
     6481 gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca
     6541 agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc
     6601 gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg
     6661 ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt
     6721 cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag
     6781 gtctctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga
     6841 acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg
     6901 atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc
     6961 ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa
     7021 aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg
     7081 cggccgctgg ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac
     7141 aagccgcgcc gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt
     7201 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     7261 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     7321 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
     7381 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     7441 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
     7501 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     7561 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     7621 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     7681 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     7741 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     7801 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     7861 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     7921 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     7981 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     8041 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     8101 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8161 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     8221 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     8281 agtggaacga aaactcacgt taagggattt tggtcatgca ttctaggtac taaaacaatt
     8341 catccagtaa aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa
     8401 aaaatagctc gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg
     8461 caatgtcata ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt
     8521 tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct
     8581 cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt
     8641 cttcttccca gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt
     8701 cggctaagcg gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa
     8761 agagcctgat gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat
     8821 actcttccga gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat
     8881 gccgttcaaa gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt
     8941 ccttttcccg ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat
     9001 ataggttttc attttctccc accagcttat ataccttagc aggagacatt ccttccgtat
     9061 cttttacgca gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag
     9121 ccatttatta tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta
     9181 taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac
     9241 agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt
     9301 gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct
     9361 ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc
     9421 ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact
     9481 gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg
     9541 ctggctggtg gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac
     9601 acattgcgga cgtttttaat gtactgaatt aacgccgaat taattcgggg gatctggatt
     9661 ttagtactgg attttggttt taggaattag aaattttatt gatagaagta ttttacaaat
     9721 acaaatacat actaagggtt tcttatatgc tcaacacatg agcgaaaccc tataggaacc
     9781 ctaattccct tatctgggaa ctactcacac attattatgg agaaactcga gcttgtcgat
     9841 cgacagatcc ggtcggcatc tactctattt ctttgccctc ggacgagtgc tggggcgtcg
     9901 gtttccacta tcggcgagta cttctacaca gccatcggtc cagacggccg cgcttctgcg
     9961 ggcgatttgt gtacgcccga cagtcccggc tccggatcgg acgattgcgt cgcatcgacc
    10021 ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag ctctgataga gttggtcaag
    10081 accaatgcgg agcatatacg cccggagtcg tggcgatcct gcaagctccg gatgcctccg
    10141 ctcgaagtag cgcgtctgct gctccataca agccaaccac ggcctccaga agaagatgtt
    10201 ggcgacctcg tattgggaat ccccgaacat cgcctcgctc cagtcaatga ccgctgttat
    10261 gcggccattg tccgtcagga cattgttgga gccgaaatcc gcgtgcacga ggtgccggac
    10321 ttcggggcag tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga cggacgcact
    10381 gacggtgtcg tccatcacag tttgccagtg atacacatgg ggatcagcaa tcgcgcatat
    10441 gaaatcacgc catgtagtgt attgaccgat tccttgcggt ccgaatgggc cgaacccgct
    10501 cgtctggcta agatcggccg cagcgatcgc atccatagcc tccgcgaccg gttgtagaac
    10561 agcgggcagt tcggtttcag gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat
    10621 gcaataggtc aggctctcgc taaactcccc aatgtcaagc acttccggaa tcgggagcgc
    10681 ggccgatgca aagtgccgat aaacataacg atctttgtag aaaccatcgg cgcagctatt
    10741 tacccgcagg acatatccac gccctcctac atcgaagctg aaagcacgag attcttcgcc
    10801 ctccgagagc tgcatcaggt cggagacgct gtcgaacttt tcgatcagaa acttctcgac
    10861 agacgtcgcg gtgagttcag gctttttcat atctcattgc cccccgggat ctgcgaaagc
    10921 tcgagagaga tagatttgta gagagagact ggtgatttca gcgtgtcctc tccaaatgaa
    10981 atgaacttcc ttatatagag gaaggtcttg cgaaggatag tgggattgtg cgtcatccct
    11041 tacgtcagtg gagatatcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc
    11101 tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc
    11161 atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc caccttcctt
    11221 ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga ggaggtttcc
    11281 cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga ctgtatcttt
    11341 gatattcttg gagtagacga gagtgtcgtg ctccaccatg ttatcacatc aatccacttg
    11401 ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc
    11461 atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg
    11521 atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc
    11581 tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc
    11641 cctttggtct tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc
    11701 caccatgttg gcaagctgct ctagccaata cgcaaaccgc ctctccccgc gcgttggccg
    11761 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
    11821 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
    11881 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
    11941 catgattacg
//
