LOCUS       sequence-47512-e         640 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Rozenblatt-Rosen O, Deo RC, Padi M, Adelmant G, Calderwood MA, 
            Rolland T, Grace M, Dricot A, Askenazi M, Tavares M, Pevzner SJ, 
            Abderazzaq F, Byrdsong D, Carvunis AR, Chen AA, Cheng J, Correll M, 
            Duarte M, Fan C, Feltkamp MC, Ficarro SB, Franchi R, Garg BK, 
            Gulbahce N, Hao T, Holthaus AM, James R, Korkhin A, Litovchick L, 
            Mar JC, Pak TR, Rabello S, Rubio R, Shen Y, Singh S, Spangle JM, 
            Tasan M, Wanamaker S, Webber JT, Roecklein-Canfield J, Johannsen E, 
            Barabasi AL, Beroukhim R, Kieff E, Cusick ME, Hill DE, Munger K, 
            Marto JA, Quackenbush J, Roth FP, DeCaprio JA, Vidal M
  TITLE     Interpreting cancer genomes using systematic host network 
            perturbations by tumour virus proteins.
  JOURNAL   Nature. 2012 Jul 26;487(7408):491-5.
  PUBMED    22810586
REFERENCE   2  (bases 1 to 640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2012-07-26"; volume: "487(7408)"; pages: "491-5"
COMMENT     Sequence Label: MSCV-C 8E6
FEATURES             Location/Qualifiers
     source          1..640
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    27..51
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(514..538)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             570..593
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             609..635
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             609..635
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 acccaagctg gctagttaag ctatcaacaa gtttgtacaa aaaagttggc atggacgggc
       61 aggacaaggc ttcatattta gacactaata aggacgagct accctctact attaaagagt
      121 tagctgcggc tttaggtatt ccattgcagg actgtttagt accgtgcaac ttttgtggta
      181 actttttgga tttcttagaa ctgtgtgagt ttgacaaaaa gagactgtgc ctaatttgga
      241 aaaattacgt tgttactgcg tgttgtcgtt gttgttgtgt agcaaccgca acgtttgaat
      301 ttaatgaata ttatgagcaa actgtgctag gcagagatat tgaattagct acaggacgtt
      361 caatttttga gatagacgtt aggtgtcaaa actgcttgtc atttttggat atcatagaga
      421 aattagattg ctgtgggaga ggccgtccct ttcataaagt tagaggaggc tggaaaggag
      481 tttgcaggct ttgtaagcat ttgtatcatg attggtcaac tttcttgtac aaagtggttg
      541 atctagaggg cccgcggttc gaactcgagg actacaagga cgacgatgac aagtcggccg
      601 ctggaggata cccctacgac gtgcccgact acgcctagaa
//
