LOCUS       sequence-47519-e         622 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Rozenblatt-Rosen O, Deo RC, Padi M, Adelmant G, Calderwood MA, 
            Rolland T, Grace M, Dricot A, Askenazi M, Tavares M, Pevzner SJ, 
            Abderazzaq F, Byrdsong D, Carvunis AR, Chen AA, Cheng J, Correll M, 
            Duarte M, Fan C, Feltkamp MC, Ficarro SB, Franchi R, Garg BK, 
            Gulbahce N, Hao T, Holthaus AM, James R, Korkhin A, Litovchick L, 
            Mar JC, Pak TR, Rabello S, Rubio R, Shen Y, Singh S, Spangle JM, 
            Tasan M, Wanamaker S, Webber JT, Roecklein-Canfield J, Johannsen E, 
            Barabasi AL, Beroukhim R, Kieff E, Cusick ME, Hill DE, Munger K, 
            Marto JA, Quackenbush J, Roth FP, DeCaprio JA, Vidal M
  TITLE     Interpreting cancer genomes using systematic host network 
            perturbations by tumour virus proteins.
  JOURNAL   Nature. 2012 Jul 26;487(7408):491-5.
  PUBMED    22810586
REFERENCE   2  (bases 1 to 622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2012-07-26"; volume: "487(7408)"; pages: "491-5"
COMMENT     Sequence Label: MSCV-N 16E6
FEATURES             Location/Qualifiers
     source          1..622
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      3..12
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             12..35
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             48..74
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    90..114
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctgccaccat ggactacaag gatgacgatg acaagctcga tggaggatac ccatacgatg
       61 ttccagatta cgctgctcga ggttatcaaa caagtttgta caaaaaagtt ggcatgcacc
      121 aaaagagaac tgcaatgttt caggacccac aggagcgacc cagaaagtta ccacagttat
      181 gcacagagct gcaaacaact atacatgata taatattaga atgtgtgtac tgcaagcaac
      241 agttactgcg acgtgaggta tatgactttg cttttcggga tttatgcata gtatatagag
      301 atgggaatcc atatgctgta tgtgataaat gtttaaagtt ttattctaaa attagtgagt
      361 atagacatta ttgttatagt ttgtatggaa caacattaga acagcaatac aacaaaccgt
      421 tgtgtgattt gttaattagg tgtattaact gtcaaaagcc actgtgtcct gaagaaaagc
      481 aaagacatct ggacaaaaag caaagattcc ataatataag gggtcggtgg accggtcgat
      541 gtatgtcttg ttgcagatca tcaagaacac gtagagaaac ccagctgtaa tctttcttgt
      601 acaaagtggt tcgataacga at
//
