LOCUS       sequence-47536-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Maine GN, Li H, Zaidi IW, Basrur V, Elenitoba-Johnson KS, Burstein E
  TITLE     A bimolecular affinity purification method under denaturing 
            conditions for rapid isolation of a ubiquitinated protein for mass 
            spectrometry analysis.
  JOURNAL   Nat Protoc. 2010 Aug;5(8):1447-59. Epub 2010 Jul 22.
  PUBMED    20671728
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2010-08"; volume: "5(8)"; pages: "1447-59. Epub 
            2010 Jul 22"
COMMENT     Sequence Label: pEBB-BT-Ubiquitin
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      59..68
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             320..340
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             386..613
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
ORIGIN
        1 tagtgtcgtg aggattctct agagatccct cgacctcgag atccattgtg ctggatctgc
       61 caccatggct ggaaaggccg gtgaaggtga aatccctgcc cctcttgctg gtaccgtttc
      121 taagatactg gtaaaagaag gtgacactgt taaagctggt caaacagttc tggtgctgga
      181 ggctatgaaa atggagacag aaattaacgc tcctactgac ggaaaagttg aaaaggtgtt
      241 agttaaggaa agagatgctg ttcaaggtgg tcaaggtcta atcaagatcg gcgactacga
      301 tatacccaca accgccagcg agaatctgta ctttcaatca gagttaaaaa ccgccgcact
      361 agctcaacat gacgaggctg gatccatgca gattttcgtg aaaaccctta cggggaagac
      421 catcaccctc gaggttgaac cctcggatac gatagaaaat gtaaaggcca agatccagga
      481 taaggaagga attcctcctg atcagcagag actgatcttt gctggcaagc agctggaaga
      541 tggacgtact ttgtctgact acaatattca aaaggagtct actcttcatc ttgtgttgag
      601 acttcgtggt ggttaagcgg ccgcgactct agagtgaggg tccccacctg ggacccttga
      661 gagtatcagg tctcccacgt gggagacaag aaatccctgt ttaatattta aacagcagtg
      721 ttccccatct gggtccttgc acccctcact ctggcctcag ccgactgcac agcggcccct
      781 gcatcccctt ggctgtgagg cccctggaca agcagaggtg gcc
//
