LOCUS       sequence-47537-e         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Maine GN, Li H, Zaidi IW, Basrur V, Elenitoba-Johnson KS, Burstein E
  TITLE     A bimolecular affinity purification method under denaturing 
            conditions for rapid isolation of a ubiquitinated protein for mass 
            spectrometry analysis.
  JOURNAL   Nat Protoc. 2010 Aug;5(8):1447-59. Epub 2010 Jul 22.
  PUBMED    20671728
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2010-08"; volume: "5(8)"; pages: "1447-59. Epub 
            2010 Jul 22"
COMMENT     Sequence Label: pEBB-TB
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             99..119
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ttctctagag atccctcgac ctcgagatcc attgtgctgg atccactagt ggtaccatcg
       61 atgcggccgc cgactacgat atacccacaa ccgccagcga gaatctgtac tttcaatcag
      121 agttaaaaac cgccgcacta gctcaacatg acgaggctgc tggaaaggcc ggtgaaggtg
      181 aaatccctgc ccctcttgct ggtaccgttt ctaagatact ggtaaaagaa ggtgacactg
      241 ttaaagctgg tcaaacagtt ctggtgctgg aggctatgaa aatggagaca gaaattaacg
      301 ctcctactga cggaaaagtt gaaaaggtgt tagttaagga aagagatgct gttcaaggtg
      361 gtcaaggtct aatcaagatc ggcgtttaag ggccgcgact ctagagtgag ggtccccacc
      421 tgggaccctt gagagtatca ggtctcccac gtgggagaca agaaatccct gtttaatatt
      481 taaacagcag tgttccccat ctgggtcctt gcacccctca ctctggcctc agccgactgc
      541 acagcggccc ctgcatcccc ttggctgtga ggcccctgga caagcagagg tggccagagc
      601 tgggaggcat ggccctgggg tcccacgaat ttgctgggga atctcgtttt tcttcttaag
      661 acttttggga catggtttga ctcccgaaca tcaccgacgt gtctcctgtt tttctgggtg
      721 gcctcgggac acctgccctg cccccacgag ggtcaggact gtgactcttt ttagggccag
      781 gcaggtgcct ggacatttgc cttgctggac ggggac
//
