LOCUS       sequence-475374-        9921 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9921)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 9921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-ZO1-C-14
FEATURES             Location/Qualifiers
     source          1..9921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      547..556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(553..555,556..558,559..1269)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(598..619)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(859..878)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1206..1227
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     RBS             2970..2978
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    6649..6770
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6686..6705)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6740..6759
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6777..7232)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6914..6935)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7126..7145
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7259..7363
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7365..7722
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7365..7556
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7388..7408)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7573..7708
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7757..8551
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7757..8551
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7811..7830)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8421..8440
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8739..8758)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8783..8830
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9159..9747
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9648..9667
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      601 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      661 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      721 ccctggccca ccctcgtgac caccttgacc tacggcgtgc agtgcttcgc ccgctacccc
      781 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      841 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      901 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      961 atcctggggc acaagctgga gtacaactac aacagccaca aggtctatat caccgccgac
     1021 aagcagaaga acggcatcaa ggtgaacttc aagacccgcc acaacatcga ggacggcagc
     1081 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1141 cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc
     1201 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1261 ctgtacaagt ccggaagtgc tgggagcgca agcggaagcg gaggctccgg tgaggaaaca
     1321 gctatatggg aacaacatac agtgacgctt cacagggctc ctggatttgg atttggaatt
     1381 gcaatatctg gtggacgaga taatcctcat tttcagagtg gggaaacgtc aatagtgatt
     1441 tcagatgtgc tgaaaggagg accagctgaa ggacagctac aggaaaatga ccgagttgca
     1501 atggttaacg gagtttcaat ggataatgtt gaacatgctt ttgctgttca gcaactaagg
     1561 aaaagtggga aaaatgcaaa aattacaatt agaaggaaga agaaagttca aataccagta
     1621 agtcgtcctg atcctgaacc agtatctgat aatgaagaag atagttatga tgaggaaata
     1681 catgatccaa gaagtggccg gagtggtgtg gttaacagaa ggagtgagaa gatttggccg
     1741 agggatagaa gtgcaagtag agagaggagc ttgtccccgc ggtcagacag gcggtcagtg
     1801 gcttccagcc agcctgctaa acctactaaa gtcacactgg tgaaatcccg gaaaaatgaa
     1861 gaatatggtc ttcgattggc aagccatata tttgttaagg aaatttcaca agatagtttg
     1921 gcagcaagag atggcaatat tcaagaaggt gatgttgtat tgaagataaa tggtactgtg
     1981 acagaaaata tgtcattgac agatgcaaag acattgatag aaaggtctaa aggcaaatta
     2041 aaaatggtag ttcaaagaga tgaacgggct acgctattga atgtccctga tctttctgac
     2101 agcatccact ctgctaatgc ctctgagaga gacgacattt cagaaattca gtcactggca
     2161 tcagatcatt ctggtcgatc acacgatagg cctccccgcc gcagccggtc acgatctcct
     2221 gaccagcggt cagagccttc tgatcattcc aggcactcgc cgcagcagcc aagcaatggc
     2281 agtctgcgga gtagagatga agagagaatt tctaaacctg gggctgtctc aactcctgta
     2341 aagcatgctg atgatcacac acctaaaaca gtggaagaag ttacagttga aagaaatgag
     2401 aaacaaacac cttctcttcc agaaccaaag cctgtgtatg cccaagttgg caaccagatg
     2461 tggatttacc tgtcagtcca tctgatggtg tcctacctaa ttcaactcat gaagatggga
     2521 tttcttcggc ccagcatgaa attggtaaaa ttcagaaaag gagatagtgt gggtttgcgg
     2581 ctggctggtg gaaatgatgt tggaatattt gtagctggcg ttctagaaga tagccctgca
     2641 gccaaggaag gcttagagga aggtgatcaa attctcaggg taaacaacgt agattttaca
     2701 aatatcataa gagaagaagc cgtccttttc ctgcttgacc tccctaaagg agaagaagtg
     2761 accatattgg ctcagaagaa gaaggatgtt tatcgtcgca ttgtagaatc agatgtagga
     2821 gattctttct atattagaac ccattttgaa tatgaaaagg aatctcccta tggacttagt
     2881 tttaacaaag gagaggtgtt ccgtgctgtg gataccttgt acaatggaaa actgggctct
     2941 tggcttgcta ttcgaattgg taaaaatcat aaggaggtag aacgaggcat catccctaat
     3001 aagaacagag ctgagcagct cgccagtgta cagtatacac ttccaaaaac agcaggcgga
     3061 gaccgtgctg acttctggag attcagaggt cttcgcagct ccaagagaaa tcttcgaaaa
     3121 agcagagagg atttgtccgc tcagcctgtt caaacaaagt ttccagctta tgaaagagtg
     3181 gttcttcgag aagctggatt tctgaggcct gtaaccattt ttggaccaat agctgatgtt
     3241 gccagagaaa agctggcaag agaagaacca gatatttatc aaattgcaaa gagtgaacca
     3301 cgagacgctg gaactgacca acgtagctct ggctatattc gcctgcatac aataaagcaa
     3361 atcatagatc aagacaaaca tgctttatta gatgtaacac caaatgcagt tgatcgtctt
     3421 aactatgccc agtggtatcc aattgttgta tttcttaacc ctgattctaa gcaaggagta
     3481 aaaacaatga gaatgaggtt atgtccagaa tctcggaaaa gtgccaggaa gttatacgag
     3541 cgatctcata aacttgctaa aaataatcac catcttttta caactacaat taacttaaat
     3601 tcaatgaatg atggttggta tggtgcgctg aaagaagcag ttcaacaaca gcaaaaccag
     3661 ctggtatggg tttccgaggg aaaggcggat ggtgctacaa gtgatgacct tgatttgcat
     3721 gatgatcgtc tgtcctacct gtcagctcca ggtagtgaat actcaatgta tagcacggac
     3781 agtagacaca cttctgacta tgaagacaca gacacagaag gcggggccta cactgatcaa
     3841 gaactagatg aaactcttaa tgatgaggtt gggactccac cggagtctgc cattacacgg
     3901 tcctctgagc ctgtaagaga ggactcctct ggaatgcatc atgaaaacca aacatatcct
     3961 ccttactcac cacaagcgca gccacaacca attcatagaa tagactcccc tggatttaag
     4021 ccagcctctc aacagaaagc agaagcttca tctccagtcc cttacctttc gcctgaaaca
     4081 aacccagcat catcaacctc tgctgttaat cataatgtaa atttaactaa tgtcagactg
     4141 gaggagccca ccccagctcc ttccacctct tactcaccac aagctgattc tttaagaaca
     4201 ccaagtactg aggcagctca cataatgcta agagatcaag aaccatcatt gtcgtcgcat
     4261 gtagatccaa caaaggtgta tagaaaggac ccatatcccg aggaaatgat gaggcagaac
     4321 catgttttga aacagccagc cgttagtcac ccagggcaca ggccagacaa agagcctaat
     4381 ctgacctatg aaccccaact cccatacgta gagaaacaag ccagcagaga cctggagcag
     4441 cccacataca gatacgagtc ctcaagctat acggaccagt tttctcgaaa ctatgaacat
     4501 cgtctgcgat acgaagatcg cgtccccatg tatgaagaac agtggtcata ttatgatgac
     4561 aaacagccct acccatctcg gccacctttt gataatcagc actctcaaga ccttgactcc
     4621 agacagcatc ccgaagagtc ctcagaacga gggtactttc cacgttttga agagccagcc
     4681 cctctgtctt acgacagcag accacgttac gaacaggcac ctagagcatc cgccctgcgg
     4741 cacgaagagc agccagctcc tgggtatgac acacatggta gactcagacc ggaagcccag
     4801 ccccaccctt cagcagggcc caagcctgca gagtccaagc agtattttga gcaatattca
     4861 cgcagttacg agcaagtacc accccaagga tttacctcta gagcaggtca ttttgagcct
     4921 ctccatggtg ctgcagctgt ccctccgctg ataccttcat ctcagcataa gccagaagct
     4981 ctgccttcaa acaccaaacc actgcctcca cccccaactc aaaccgaaga agaggaagat
     5041 ccagcaatga agccacagtc tgtactcacc agagttaaga tgtttgaaaa caaaaggtct
     5101 gcatccttag agaccaagaa ggatgtaaat gacactggca gttttaagcc tccagaagta
     5161 gcatctaaac cttcaggtgc tcccatcatt ggtcccaaac ccacttctca gaatcaattc
     5221 agtgaacatg acaaaactct gtacaggatt ccagaacctc aaaaacctca actgaagcca
     5281 cctgaagata ttgttcggtc caatcattat gaccctgaag aagatgaaga atattatcga
     5341 aaacagctgt catactttga ccgaagaagt tttgagaata agcctcctgc acacattgct
     5401 gccagccatc tctccgagcc tgcaaagcca gctcattctc agaatcaatc aaatttttct
     5461 agttattctt caaagggaaa gcctcctgaa gctgatggtg tggatagatc atttggcgag
     5521 aaacgctatg aacccatcca ggccactccc cctcctcctc cattgccctc gcagtatgcc
     5581 cagccatctc agcctgtcac cagcgcgtct ctccacatac attctaaggg agcacatggt
     5641 gaaggtaatt cagtgtcatt ggattttcag aattccttag tgtccaaacc agacccacct
     5701 ccatctcaga ataagccagc aactttcaga ccaccaaacc gagaagatac tgctcaggca
     5761 gctttctatc cccagaaaag ttttccagat aaagccccag ttaatggaac tgaacagact
     5821 cagaaaacag tcactccagc atacaatcga ttcacaccaa aaccatatac aagttctgcc
     5881 cgaccatttg aacgcaagtt tgaaagtcct aaattcaatc acaatcttct gccaagtgaa
     5941 actgcacata aacctgactt gtcttcaaaa actcccactt ctccaaaaac tcttgtgaaa
     6001 tcgcacagtt tggcacagcc tcctgagttt gacagtggag ttgaaacttt ctctatccat
     6061 gcagagaagc ctaaatatca aataaataat atcagcacag tgcctaaagc tattcctgtg
     6121 agtccttcag ctgtggaaga ggatgaagat gaagatggtc atactgtggt ggccacagcc
     6181 cgaggcatat ttaacagcaa tgggggcgtg ctgagttcca tagaaactgg tgttagtata
     6241 attatccctc aaggagccat tcccgaagga gttgagcagg aaatctattt caaggtctgc
     6301 cgggacaaca gcatccttcc acctttagat aaagagaaag gtgaaacact gctgagtcct
     6361 ttggtgatgt gtggtcccca tggcctcaag ttcctgaagc ctgtggagct gcgcttacca
     6421 cactgtgatc ctaaaacctg gcaaaacaag tgtcttcccg gagatccaaa ttatctcgtt
     6481 ggagcaaact gtgtttctgt ccttattgac cacttttaag gatccaccgg atctagataa
     6541 ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     6601 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     6661 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     6721 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta acgcgtaaat
     6781 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     6841 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     6901 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     6961 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     7021 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     7081 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     7141 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     7201 cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg
     7261 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     7321 ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac
     7381 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     7441 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     7501 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     7561 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     7621 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     7681 aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg
     7741 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     7801 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     7861 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     7921 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     7981 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     8041 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     8101 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     8161 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     8221 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     8281 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     8341 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     8401 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     8461 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     8521 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     8581 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     8641 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     8701 atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata
     8761 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg
     8821 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
     8881 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
     8941 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca
     9001 ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     9061 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     9121 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     9181 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     9241 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     9301 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     9361 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     9421 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     9481 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     9541 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     9601 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     9661 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     9721 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     9781 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     9841 aaccgtatta ccgccatgca ttagttatta atagtaatca attacggggt cattagttca
     9901 tagcccatat atggagttcc g
//
