LOCUS       sequence-47538-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  TITLE     pEBB-SBP-HA
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEBB-SBP-HA
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      45..54
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             51..164
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             198..224
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             231..257
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ttctctagag atccctcgac ctcgagatcc attgtgctgg atctgccacc atggacgaga
       61 agaccaccgg ctggagaggc ggccacgtgg tcgaggggct agccggtgag ctggagcagc
      121 tacgggcccg ccttgagcac cacccccagg ggcagcgcga gccgggatcc actagtggta
      181 ccatcgatgc ggccgcctac ccatacgatg ttccagatta cgctggtggc tacccatacg
      241 atgttccaga ttacgcttga gggccgcgac tctagagtga gggtccccac ctgggaccct
      301 tgagagtatc aggtctccca cgtgggagac aagaaatccc tgtttaatat ttaaacagca
      361 gtgttcccca tctgggtcct tgcacccctc actctggcct cagccgactg cacagcggcc
      421 cctgcatccc cttggctgtg aggcccctgg acaagcagag gtggccagag ctgggaggca
      481 tggccctggg gtcccacgaa tttgctgggg aatctcgttt ttcttcttaa gacttttggg
      541 acatggtttg actcccgaac atcaccgacg tgtctcctgt ttttctgggt ggcctcggga
      601 cacctgccct gcccccacga gggtcaggac tgtgactctt tttagggcca ggcaggtgcc
      661 tggacatttg ccttgctgga cggggactgg ggatgtggga gggagcagac aggaggaatc
      721 atgtcaggcc tgtgtgtgaa aggaagctcc actgtcaccc tccacctctt caccccc
//
