LOCUS       sequence-47539-e         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Maine GN, Li H, Zaidi IW, Basrur V, Elenitoba-Johnson KS, Burstein E
  TITLE     A bimolecular affinity purification method under denaturing 
            conditions for rapid isolation of a ubiquitinated protein for mass 
            spectrometry analysis.
  JOURNAL   Nat Protoc. 2010 Aug;5(8):1447-59. Epub 2010 Jul 22.
  PUBMED    20671728
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2010-08"; volume: "5(8)"; pages: "1447-59. Epub 
            2010 Jul 22"
COMMENT     Sequence Label: pEBB-BT
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      45..54
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             306..326
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ttctctagag atccctcgac ctcgagatcc attgtgctgg atctgccacc atggctggaa
       61 aggccggtga aggtgaaatc cctgcccctc ttgctggtac cgtttctaag atactggtaa
      121 aagaaggtga cactgttaaa gctggtcaaa cagttctggt gctggaggct atgaaaatgg
      181 agacagaaat taacgctcct actgacggaa aagttgaaaa ggtgttagtt aaggaaagag
      241 atgctgttca aggtggtcaa ggtctaatca agatcggcga ctacgatata cccacaaccg
      301 ccagcgagaa tctgtacttt caatcagagt taaaaaccgc cgcactagct caacatgacg
      361 aggctggatc cactagtggt accatcgatg cggccgcgac tctagagtga gggtccccac
      421 ctgggaccct tgagagtatc aggtctccca cgtgggagac aagaaatccc tgtttaatat
      481 ttaaacagca gtgttcccca tctgggtcct tgcacccctc actctggcct cagccgactg
      541 cacagcggcc cctgcatccc cttggctgtg aggcccctgg acaagcagag gtggccagag
      601 ctgggaggca tggccctggg gtcccacgaa tttgctgggg aatctcgttt ttcttcttaa
      661 gacttttggg acatggtttg actcccgaac atcaccgacg tgtctcctgt ttttctgggt
      721 ggcctcggga cacctgccct gcccccacga gggtcaggac tgtgactctt tttagg
//
