LOCUS       sequence-47541-e        2555 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET-21a(+)/MBP-His-PINK1
FEATURES             Location/Qualifiers
     source          1..2555
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(649..660)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1153..1170
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 catatgaaaa ttgaagaagg taaactggtc atctggatta acggtgataa aggctacaac
       61 ggtctggctg aagtgggtaa aaaattcgaa aaagataccg gcattaaagt gacggttgaa
      121 catccggaca aactggaaga aaaatttccg caggtggcag caaccggtga tggtccggac
      181 attatctttt gggcacacga tcgtttcggc ggttatgccc agagtggcct gctggcagaa
      241 atcacgccgg ataaagcctt tcaagacaaa ctgtacccgt tcacctggga tgctgttcgc
      301 tataatggta aactgattgc ctacccgatc gcagtcgaag ctctgagtct gatttataac
      361 aaagatctgc tgccgaatcc gccgaaaacg tgggaagaaa tcccggcgct ggacaaagaa
      421 ctgaaagcta agggtaaatc cgcgctgatg tttaacctgc aggaaccgta tttcacctgg
      481 ccgctgattg cagctgatgg cggttatgcc ttcaaatacg aaaacggcaa atacgatatc
      541 aaagacgtcg gtgtggataa cgcgggcgcc aaagcaggtc tgacgtttct ggttgacctg
      601 atcaaaaaca aacacatgaa cgctgatacc gactatagta tcgcggaagc ggccttcaat
      661 aaaggcgaaa ccgctatgac gattaacggt ccgtgggcgt ggagtaatat cgatacctcc
      721 aaagtgaact acggcgttac cgtcctgccg acgtttaagg gtcagccgtc caaaccgttc
      781 gtgggcgttc tgagcgccgg tattaacgca gcttctccga ataaagaact ggcaaaagaa
      841 tttctggaaa attatctgct gaccgatgaa ggcctggaag cggtcaacaa agacaaaccg
      901 ctgggtgctg tggcgctgaa aagctatgaa gaagaactgg ttaaagatcc gcgtattgcg
      961 gccacgatgg aaaatgccca gaaaggcgaa attatgccga acatcccgca aatgagtgcg
     1021 ttctggtatg ccgtccgtac cgcagtgatc aatgcagctt ccggtcgcca gacggtggat
     1081 gaagctctga aagacgcgca aaccaacagc tctagtaaca ataataataa taacaacaac
     1141 aacaatctgg gccatcacca tcaccatcac tgccaggaaa ttcaagcgat ctttacgcag
     1201 aaatctaaac cgggtccgga cccgctggac acccgtcgcc tgcagggctt ccgtctggaa
     1261 gaatatctga ttggtcaatc aatcggcaaa ggttgttcgg cggccgtgta cgaagcgacc
     1321 atgccgacgc tgccgcagaa tctggaagtt accaaatcaa cgggcctgct gccgggtcgt
     1381 ggtccgggta cctcggcacc gggtgaaggt caagaacgtg caccgggtgc accggcattt
     1441 ccgctggcga ttaaaatgat gtggaatatc agcgctggtt cctcatcgga agcgattctg
     1501 aacacgatga gtcaggaact ggtcccggca tcccgtgtgg ctctggcagg cgaatatggt
     1561 gcggttacct accgtaaatc taaacgtggt ccgaaacagc tggcaccgca tccgaacatt
     1621 atccgtgtgc tgcgcgcatt taccagctct gttccgctgc tgccgggtgc gctggttgat
     1681 tatccggacg tcctgccgtc acgtctgcat ccggaaggtc tgggtcacgg tcgtaccctg
     1741 ttcctggtca tgaaaaatta tccgtgcacg ctgcgtcagt acctgtgtgt gaacaccccg
     1801 tcgccgcgtc tggcagctat gatgctgctg caactgctgg aaggcgttga tcatctggtc
     1861 cagcaaggta ttgcgcaccg tgatctgaaa agcgacaaca tcctggtgga actggacccg
     1921 gacggttgcc cgtggctggt tattgccgat tttggctgct gtctggcaga cgaatctatc
     1981 ggtctgcagc tgccgttcag ttcctggtat gtggatcgcg gcggtaatgg ctgtctgatg
     2041 gcaccggaag ttagcaccgc acgtccgggt ccgcgtgctg tcattgatta ctctaaagca
     2101 gacgcatggg ctgtcggtgc aattgcctat gaaatctttg gtctggtgaa cccgttctac
     2161 ggccagggta aagcgcacct ggaatcacgt tcgtatcagg aagcccaact gccggcactg
     2221 ccggaaagcg tgccgccgga tgttcgtcag ctggtccgtg cactgctgca acgtgaagca
     2281 agcaaacgtc cgtctgcacg cgtggcggcc aatgttctgc atctgtcact gtggggcgaa
     2341 cacattctgg cgctgaaaaa cctgaaactg gataaaatgg ttggttggct gctgcagcaa
     2401 tcggcagcta ccctgctggc gaatcgcctg acggaaaaat gctgtgtgga aaccaaaatg
     2461 aaaatgctgt ttctggccaa cctggaatgt gaaaccctgt gtcaagctgc cctgctgctg
     2521 tgctcgtggc gtgctgctct gtaatgagcg gccgc
//
