LOCUS       sequence-475412-        6892 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Membrane Scaffold, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6892)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 6892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-PSD95-N-14
FEATURES             Location/Qualifiers
     source          1..6892
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      559..568
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2773..2782
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2779..2781,2782..2784,2785..3498)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2824..2845)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3085..3104)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3432..3453
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3620..3741
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3657..3676)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3711..3730
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3748..4203)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3885..3906)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4097..4116
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4230..4334
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4336..4693
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4336..4527
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4359..4379)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4544..4679
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4728..5522
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4728..5522
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4782..4801)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5392..5411
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5710..5729)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5754..5801
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6130..6718
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6619..6638
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctcgaggc caccatggac tgtctctgta tagtgacaac caagaaatac
      601 cgctaccaag atgaagacac gccccctctg gaacacagcc cggcccacct ccccaaccag
      661 gccaattctc cccctgtgat tgtcaacacg gacaccctag aagccccagg atatgagttg
      721 caggtgaatg gaacagaggg ggagatggag tatgaggaga tcacattgga aaggggtaac
      781 tcaggtctgg gcttcagcat cgcaggtggc actgacaacc cgcacatcgg tgacgacccg
      841 tccattttta tcaccaagat cattcctggt ggggctgcag cccaggatgg ccgcctcagg
      901 gtcaatgaca gcatcctgtt tgtaaatgaa gtggatgttc gggaggtgac ccattcagct
      961 gcggtggagg ccctcaaaga ggcaggttcc atcgttcgcc tctatgtcat gcgccggaaa
     1021 cccccagccg aaaaggtcat ggagatcaaa ctcatcaaag ggcctaaagg acttggcttc
     1081 agcattgcgg ggggcgttgg gaaccagcac atccctggag ataacagcat ctatgtaacg
     1141 aagatcatcg aaggaggtgc tgcccacaag gatggcaggt tgcagattgg agacaagatc
     1201 ctggcggtca acagtgtggg gctggaggac gtcatgcacg aggatgccgt ggcagccctg
     1261 aagaacacat atgacgttgt gtacctaaag gtggccaagc ccagcaatgc ctacctgagt
     1321 gacagctatg ctcccccaga catcacaacc tcgtattctc agcacctgga caatgagatc
     1381 agtcatagca gctacttggg cactgactac cccacagcca tgacccccac ttcccctcgg
     1441 cgctactccc ctgtggccaa ggacctgctg ggggaggaag acattccccg ggaaccaagg
     1501 cggatcgtga tccatcgggg ctccaccggc ctgggcttca acatcgtggg cggcgaggat
     1561 ggtgaaggca tcttcatctc cttcatcctt gctgggggtc cagccgacct cagtggggag
     1621 ctacggaagg gggaccagat cctgtcggtc aatggtgttg acctccgcaa tgccagtcac
     1681 gaacaggctg ccattgccct gaagaatgcg ggtcagacgg tcacgatcat cgctcagtat
     1741 aaaccagaag agtatagtcg attcgaggcc aagatccatg atcttcggga acagctcatg
     1801 aatagtagcc taggctcagg gactgcatcc ttgcgaagca accccaagag gggcttctac
     1861 attagggccc tgtttgatta cgacaagacc aaggactgcg gtttcttgag ccaggccctg
     1921 agcttccgct tcggggatgt gcttcatgtc attgacgctg gtgacgaaga gtggtggcaa
     1981 gcacggcggg tccactccga cagtgagacc gacgacattg gcttcattcc cagcaaacgg
     2041 cgggtcgagc gacgagagtg gtcaaggtta aaggccaagg actggggctc cagctctgga
     2101 tcacagggtc gagaagactc ggttctgagc tatgagacgg tgacccagat ggaagtgcac
     2161 tatgctcgtc ccatcatcat ccttggaccc accaaagacc gtgccaacga tgatcttctc
     2221 tccgagttcc ccgacaagtt tggatcctgt gtccctcata cgacacgtcc taagcgggaa
     2281 tatgagatag acggccggga ttaccacttt gtctcctccc gggagaaaat ggagaaggac
     2341 atccaggcac acaagttcat tgaggctggc cagtacaaca gccacctcta tgggaccagc
     2401 gtccagtctg tgcgagaggt agcagagcag gggaagcact gcatcctcga tgtctcggcc
     2461 aatgccgtgc ggcggctgca ggcggcccac ctgcacccca tcgccatctt catccgtccc
     2521 cgctccctgg agaatgtgct agagatcaat aagcggatca cagaggagca agcccggaaa
     2581 gccttcgaca gagccacgaa gctggagcag gagttcacag agtgcttctc agccatcgta
     2641 gagggcgaca gctttgaaga gatctatcac aaagtgaaac gtgtcattga agacctctca
     2701 ggcccctaca tctgggtccc agcccgagag agactcggca gcgcatccgg cggaagcgga
     2761 agcggaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     2821 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     2881 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     2941 cccgtgccct ggcccaccct cgtgaccacc ttgacctacg gcgtgcagtg cttcgcccgc
     3001 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     3061 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     3121 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     3181 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaaggt ctatatcacc
     3241 gccgacaagc agaagaacgg catcaaggtg aacttcaaga cccgccacaa catcgaggac
     3301 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     3361 ctgctgcccg acaaccacta cctgagcacc cagtccaagc tgagcaaaga ccccaacgag
     3421 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     3481 gacgagctgt acaagtaagc ggccgcgact ctagatcata atcagccata ccacatttgt
     3541 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     3601 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     3661 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     3721 caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg
     3781 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     3841 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     3901 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     3961 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     4021 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     4081 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     4141 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     4201 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     4261 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     4321 gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
     4381 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     4441 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     4501 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     4561 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     4621 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     4681 aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     4741 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     4801 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     4861 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag
     4921 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     4981 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     5041 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     5101 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     5161 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     5221 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg
     5281 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     5341 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     5401 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     5461 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     5521 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     5581 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     5641 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg
     5701 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
     5761 aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
     5821 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt
     5881 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa
     5941 cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt
     6001 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     6061 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6121 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6181 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6241 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     6301 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6361 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6421 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6481 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6541 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6601 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     6661 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6721 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     6781 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc attagttatt
     6841 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cg
//
