LOCUS       sequence-47542-e        1184 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET-21a(+)/MBP-His
FEATURES             Location/Qualifiers
     source          1..1184
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(649..660)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      961..970
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1153..1170
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 catatgaaaa tcgaagaagg caaactggtc atctggatca atggcgacaa aggctacaat
       61 ggtctggcgg aagtgggcaa aaaattcgaa aaagataccg gcattaaagt gacggttgaa
      121 catccggaca aactggaaga aaaatttccg caggtggcag caaccggtga tggtccggac
      181 attatctttt gggcgcacga tcgtttcggc ggttatgcac agtctggcct gctggctgaa
      241 atcaccccgg ataaagcctt tcaagacaaa ctgtacccgt tcacgtggga tgcagtgcgc
      301 tataatggta aactgattgc gtacccgatc gccgttgaag cactgtccct gatttataac
      361 aaagatctgc tgccgaatcc gccgaaaacc tgggaagaaa tcccggcgct ggacaaagaa
      421 ctgaaagcga agggtaaatc agccctgatg tttaacctgc aggaaccgta tttcacgtgg
      481 ccgctgattg cagctgatgg cggttacgcg ttcaaatacg aaaacggcaa atacgatatc
      541 aaagacgtcg gtgtggataa cgcgggcgcc aaagcaggtc tgacctttct ggtcgacctg
      601 attaaaaaca aacacatgaa tgcggatacg gactattcga tcgccgaagc ggccttcaat
      661 aaaggcgaaa ccgcaatgac gattaacggt ccgtgggctt ggtccaatat cgatacctca
      721 aaagttaact acggcgttac cgtcctgccg acgtttaaag gtcaaccgag caaaccgttc
      781 gtgggcgttc tgtcggccgg tattaacgca gctagcccga ataaagaact ggcaaaagaa
      841 tttctggaaa attatctgct gaccgatgaa ggcctggaag cggttaacaa agacaaaccg
      901 ctgggtgctg tcgcgctgaa aagctatgaa gaagaactgg tgaaagatcc gcgtattgcg
      961 gccaccatgg aaaatgccca gaaaggcgaa attatgccga acatcccgca aatgtctgct
     1021 ttctggtatg cggtccgtac ggccgtgatc aatgcagcta gtggtcgcca gaccgttgat
     1081 gaagcactga aagacgctca aacgaacagc tctagtaata ataataataa caataacaat
     1141 aataatctgg gtcatcatca tcatcatcac taataagcgg ccgc
//
