LOCUS       sequence-47545-e        1184 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFastBac1/MBP-His
FEATURES             Location/Qualifiers
     source          1..1184
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      964..973
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1156..1173
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ggatccatga agatcgagga aggcaagctg gtcatctgga tcaacggaga caagggctac
       61 aacggactgg ctgaggtcgg caagaagttc gagaaggaca ctggtatcaa ggtgacagtc
      121 gagcaccccg ataagctcga ggaaaagttc cctcaggtgg ctgctaccgg cgacggaccc
      181 gatatcatct tctgggctca cgacaggttc ggtggctacg ctcagtccgg actgctcgcc
      241 gaaatcacac cagacaaggc cttccaagat aagctctacc cgttcacctg ggacgctgtg
      301 agatacaacg gcaagttgat cgcctaccct atcgccgtcg aggctctgtc actcatctac
      361 aacaaggact tgctgcccaa cccccctaag acatgggagg aaatccctgc tttggataag
      421 gaactgaagg ctaagggcaa gtcggccctc atgttcaact tgcaggagcc atacttcact
      481 tggccgctga tcgctgccga cggaggttac gctttcaagt acgagaacgg caagtacgac
      541 atcaaggatg ttggagtgga caacgctggt gccaaggctg gcttgacttt cctggttgat
      601 ctcatcaaga acaagcacat gaacgctgac acagattaca gcatcgccga agctgccttc
      661 aacaagggcg agaccgctat gactatcaac ggcccttggg cctggagcaa catcgacacc
      721 tcaaaggtca actacggagt cacagttctg ccaaccttca agggtcaacc atccaagccg
      781 ttcgtgggag tcctctcggc tggtatcaac gctgcctccc ccaacaagga actggccaag
      841 gaattcctcg agaactacct cttgactgac gaaggcttgg aggctgtcaa caaggataag
      901 ccattgggag ccgttgctct gaagtcttac gaggaagagc tggtgaagga cccgcgcatc
      961 gctgccacca tggaaaacgc ccagaagggc gagatcatgc ccaacatccc tcaaatgtct
     1021 gccttctggt acgctgttcg tactgccgtg atcaacgctg cctccggtcg tcagaccgtc
     1081 gacgaggctc tgaaggatgc ccaaactaac tcctctagca acaacaacaa caacaacaac
     1141 aacaacaact tgggtcacca ccaccaccac cactaagcgg ccgc
//
