LOCUS       sequence-475537-       11937 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express NUP98 in bicistronic retroviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11937)
  AUTHORS   Michmerhuizen NL, Heikamp EB, Iacobucci I, Umeda M, Arthur B, Mishra
            V, Park CS, Di Giacomo D, Hiltenbrand R, Gao Q, Radko-Juettner S, 
            Lott J, Martucci C, Subramanyam V, Hatton C, Wenge DV, Baviskar P, 
            Portola P, Claquin A, Chandra B, Baggett DW, Khalighifar A, Huang H,
            Zhou P, Long L, Shi H, Sun Y, Papachristou EK, Sekhar Reddy 
            Chilamakuri C, de Luna Vitorino FN, Gongora JM, Wu H, Pounds SB, 
            Janke LJ, Kentsis A, D'Santos CS, Garcia BA, Kriwacki RW, Chi H, 
            Klco JM, Armstrong SA, Mullighan CG
  TITLE     KAT6A and KAT7 Histone Acetyltransferase Complexes Are Molecular 
            Dependencies and Therapeutic Targets in NUP98-Rearranged Acute 
            Myeloid Leukemia.
  JOURNAL   Cancer Discov. 2025 Jun 19. doi: 10.1158/2159-8290.CD-24-1772.
  PUBMED    40536430
REFERENCE   2  (bases 1 to 11937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2025 Jun 19. doi: 10.1158/2159-8290.CD-24-1772."
COMMENT     Sequence Label: CL20_MSCV-NUP98-ires-GFP
FEATURES             Location/Qualifiers
     source          1..11937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(95..114)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      908..1496
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1397..1416
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1650..1667
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1784..1805
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1820..1825,1826..1843,1844..1850)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1858..1874
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     LTR             2036..2550
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     CDS             complement(join(2652..3365,3366..3368,3369..3371))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2697..2718)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3046..3065
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3305..3326
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    3383..3935
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3385..3952
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3545..3564)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     3774..3791
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     protein_bind    3965..3989
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9426..9450)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(9477..9893)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(9493..9509)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(9525..9547)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    9960..10301
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     LTR             10365..10881
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(11081..11100)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(11228..11250)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(11444..11463)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11563..11585
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11623..11641)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11709..11813
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11814..11882,11883..11937,1..737)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
       61 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
      121 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
      181 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
      241 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
      301 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
      361 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
      421 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
      481 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
      541 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
      601 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
      661 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
      721 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
      781 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
      841 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
      901 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
      961 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1021 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     1081 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1141 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1201 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1261 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1321 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1381 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1441 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     1501 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     1561 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     1621 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     1681 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     1741 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     1801 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     1861 tgagcggata acaatttcac aattctcccc agctctttct gcgctgtcca taatgaacta
     1921 tttcaggagt attggagtat gtggattcaa aacagtggct aattgagtca ggattactta
     1981 atattcgact cctaccctca gaatgttgtt ctccaacttc aagaaactgt tacccatgaa
     2041 agacccccgc tgacgggtag tcaatcactc agaggagacc ctcccaagga acagcgagac
     2101 cacaagtcgg atgcaactgc aagagggttt attggataca cgggtacccg ggcgacgcag
     2161 tctatcggag gactggcgcg ccgagtgagg ggttgtgggc tcttttattg agctcgggga
     2221 gcagaagcgc gcgaacagaa gcgagaagcg aactgattgg ttagttcaaa taaggcacag
     2281 ggtcatttca ggtccttggg gcaccctgga aacatctgat ggttctctag aaactgctga
     2341 gggcgggacc gcatctgggg accatctgtt cttggccctg agccggggca ggaactgctt
     2401 accacagata tcctgtttgg cccatattct gctgtctctc tgttcctaac cttgatctga
     2461 acttctctat tctcagttat gtattttcca tgccttgcaa aatggcgtta cttaagctag
     2521 cttgccaaac ctacaggtgg ggtctttcat tccccccttt ttctggagac taaataaaat
     2581 cttttatttt atcgataagc ttggctgcag gtcgacggta tcgataagct tgataattct
     2641 taattaattc attacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg
     2701 aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg
     2761 gtgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc
     2821 tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc
     2881 gccttgatgc cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg
     2941 tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg
     3001 atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg
     3061 tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag
     3121 aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc actgcacgcc gtaggtcagg
     3181 gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat gaacttcagg
     3241 gtcagcttgc cgtaggtggc atcgccctcg ccctcgccag acacgctgaa cttgtggccg
     3301 tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc
     3361 ttgctcacca tggttgtggc catattatca tcgtgttttt caaaggaaaa ccacgtcccc
     3421 gtggttcggg gggcctagac gtttttttaa cctcgactaa acacatgtaa agcatgtgca
     3481 ccgaggcccc agatcagatc ccatacaatg gggtaccttc tgggcatcct tcagcccctt
     3541 gttgaatacg cttgaggaga gccatttgac tctttccaca actatccaac tcacaacgtg
     3601 gcactggggt tgtgccgcct ttgcaggtgt atcttataca cgtggctttt ggccgcagag
     3661 gcacctgtcg ccaggtgggg ggttccgctg cctgcaaagg gtcgctacag acgttgtttg
     3721 tcttcaagaa gcttccagag gaactgcttc cttcacgaca ttcaacagac cttgcattcc
     3781 tttggcgaga ggggaaagac ccctaggaat gctcgtcaag aagacagggc caggtttccg
     3841 ggccctcaca ttgccaaaag acggcaatat ggtggaaaat aacatataga caaacgcaca
     3901 ccggccttat tccaagcggc ttcggccagt aacgttaggg ggggggggcg gaattcgtta
     3961 tcgaaccact ttgtacaaga aagctgggtt gcggccgcac tcgagctaca ggctcccaac
     4021 agccagttct cgcagatagg actgggtaag gctgcgcagt tcgtccatgg catagtcctc
     4081 aggcatggga agccggccaa tgtggggagc caagaggcgc aaagggactc gctgagggtc
     4141 tggtgttgag tcggaggttc tatcaggagg atgatgcaga ctcagcacca cgcgcagcag
     4201 gttggctaca cgtttggcca tgtctgactg agccaggcga tctttagcac tgtaacactg
     4261 aatctgctct atccgactgc acagtgaagt cactttgatg tgtaactgct ccaggtcatt
     4321 acctgagcaa tccacctgct gtatatggcg gagcatttca atgactctaa tatagtccag
     4381 gtaaacaagc ccagatgttt cccaatcctg aattaggctg ctgcgctctg gaggtgccag
     4441 gtcttccaag aaccccttca ggtagtcata gttctcatta atgatggcat cagaagctaa
     4501 gtgtcggatg atgagcttgt ggcagcggtt ccagtgctca gccttaaata agcaaagggc
     4561 ctctaagtgc ttgtcagatt ccatgtgtgc tcgcacagct ttggcctcgt ggatccattt
     4621 ggcaggtaca cggagcttct gggtaaggaa agtctcttta gcccaagatt caggggtctc
     4681 caacagctgg cagtgccggg taagcagctc tcgaacagcc ttctcacgta tgcctgagtt
     4741 gtcaatgtgc aggaggacaa agatggccca ctcccagagc ccctcacttt caagctggcc
     4801 agcgtaactg gcctgtagca caccttcaca ctgcgctgag agatgggtgt agttaagagc
     4861 cctcagcact tcccacaagt gccagcttag gcggtagtcc aaaggatctg ctgttatgct
     4921 tcgaggctcc agcagctggt tgagatcata atgtctgtca ctgtagagtt ttagaaggtg
     4981 aaagcagaca tctcgaagtg gtgtctgtga gttttgctcc tccgctatca cacagccaga
     5041 accctccaga tacgaaggaa gtggggagca ggcatatctg tcactgtcag aggtattctg
     5101 aaatgcttct tcatacatgc tgagcgccct agaaatggag gctgttggtg gaagcaaata
     5161 ccaaagatgg atagccaggg agcgtttcca atccaactgg gagcacacgt ttatttgctt
     5221 cttttctgag agctgccaca ccggttttcc agccaacaga gcaaagatgc gcagtctctc
     5281 atcctggatg aaggagtctg cttggagctg atgccagtcc accaactgca tggtgagcag
     5341 ctcccggact gactggctac ccacaaactg agataaaaga agagcaagac gatgatcccc
     5401 tgactgctgg gccagagagc aggcctcact gatccttttg cctgtgaggt agctgaatac
     5461 agcctccaca gggctgtttt tttgggttaa ggagacttcc tcttcaatct gaggtgtggc
     5521 agtacaggat agccagcggg agaaagctct tcttcgctcc agaatttgaa tgtattcacg
     5581 gggttcattt agctggctgt caagctcctt caggtggccc catagggctt cacatagtgt
     5641 ccatgtcagg ctccagtgct tcacaatttg tgcttctggt aagtctcctg atgcttcttt
     5701 aacccaatct gcatagtcat gaatgacagc aactcccaga ttggggacaa tgagaggaca
     5761 cagttcatcc acatggacag tgctgtgttt taatttgagc tccagaggtg tctgatataa
     5821 tttcatgtct tcatctggct ttctttgtct caaactgagt ttttctaagt gaactttgaa
     5881 tggggactca gttaggggtt taacagctac tggattgggc aggaatccaa actccatgga
     5941 atcggcaatc tgatgatttt ctagttcatg agagccattc agctgttctc cactattagc
     6001 aagagtccag ttggggcccc aaccaacacg aaatgagcgt cccatgaata gggccatgtc
     6061 catcaagagt tttcccttgc cataggtgac agacttttca cgagggacta ggcctagttg
     6121 cctacgtgta cccactgttt tcaacggaac ctcaggggct gggctgggca ttgtgaacac
     6181 agaagtcagg ggtggaggga cagaccaaga ggatgtggat gggatattca ttaaagatgc
     6241 tgctctggga gtacgacatt cttgcacaga gacacttggt gaaagaaaag ctccacttgt
     6301 aaattttgat tgtagtaacc caccaactag tgaacgagtt ttcgacgagt gggatgctga
     6361 aatggggagt ctgggagaac agatttcttg agaagtatct gctttggaag gcaggcgact
     6421 gaagcgttga tccagtgcca tatctacatc ttcttcatca gtaagcaatg atgctttcat
     6481 gatctgtaag acatgtggat taattcccag tgaagatgca atatgtgttg aggcagacac
     6541 aggttcctga tcctcaggca tgctctcttc taacatggta tccaaaactg gctcctgggt
     6601 gatatctacc atgtcactgt ccagttccac aaccctccct aactgctcca cctctgggct
     6661 ctggctctga ggtggaggtg caggtttgcc attaagagcc atctgcaagg gcgtagtctg
     6721 gcttgcagga ggcaaaggag cagtcttcaa cttctttgta ctagttttag acggatgctc
     6781 ctcctcctct tcatcagaat cctgaaggcc atacttagaa aaatgggaga ccttaaacac
     6841 ccaagaacca gtttcaggcc ggtattcttt gaattgagct ccctgtttcc ttgaaactgc
     6901 ttccaatctt ccttcatagt tgatatcagc aaggcgatct gggctcttta ttaaacaacg
     6961 agatgtttta tctgttggcc aaactccatc caatgtaact tcagccttcc tatttagccc
     7021 ttcacccaca ggtggttttt ggttatcatc taagtagaca actacttctt tcctccggat
     7081 atgcacaata tcatccaaat ttagatttgt caaattcaca tctccttcaa aatagattga
     7141 accataacct ttccgaccaa tagtgaaatc agagacaatg cactctcctt tttcattggt
     7201 aattttagca aggtcatcca tagatggaat agtatagtaa ccaaccttag tgagaataat
     7261 acctgctggg tgcatatggt aagaattatt ttctatttct tctcggtcat cctgaagtga
     7321 ctcatcatga aaagacgttt cttcactgct tccttccagc ccatttcgca aagcagcacg
     7381 catgtttaat gcaacaatgg tatcatccac actgttgctg ttgctgtgtt tatttccagc
     7441 actttctggg gtttgaggaa taggtttggc aatagggtta gtataaaaat gtgaaacaag
     7501 ggaatcttca tctccatcct gctggtgatt ctcatcaaca ggtttgctta ggaaactaaa
     7561 tctctctcca ttttctggat attcagatgg tgaagctaga ttttctgaat cacgattaac
     7621 aggagaaaag agattgctat tattaaggtt cttcaaaacc aacttcttaa tgctcttctt
     7681 gggcatgaat gctccattgg ctagggatgg ttcatcgtca tccagcccat caaagagatg
     7741 tgacttggct gtgcctgttg tttgtaaagc ctttggccgg actctagtgg cagggcgggg
     7801 tgtcagttta taatgagtag gtgtagtaag agccttctgg gctgctggat ttgttggttt
     7861 caatctctct tccttcttct tagggtctga catcggattc cggaagagag gagagtctcc
     7921 aaaaggtgag tatgttagac tattgatgtg ctgctggaga acagcctgct gggcagcaga
     7981 agcatttgga tctgtcaaag ctactggggc ctggggggct ccaaagccca aagtggctgt
     8041 cgtagtatta aatccagggg ccccaaaggc tcctgtacca agaggccctc caatcttagg
     8101 ttggttgttc ccaaacaaag atgcctgtcc agcaccaaga gctgttccaa atcctgcacc
     8161 aagcccagtt ccaagagtcc caggtgctgg tttacttcca aaaatactat tcccactggt
     8221 atttgtgcca aaaccaaaga gcccgccact ggttgtacca aatgaaggtg cactggttgt
     8281 gctgcttcca aatgtagtaa gcttgttatt gccaaacagg gtcgaaccaa cagcaccaaa
     8341 tccagtattg gtctgcccaa agagacctgt tcctgttcca aatgctgtcc cagtgctggt
     8401 gtttgtagct gtcccaaaaa gacctccagg ctgtgaggct tgggttactc caaataatcc
     8461 catggtgttg gtgcttggct gtcctatggt gctggtatta ccaaaggaaa agccagtgtt
     8521 ctgggtggtt gtagcctggc caaatggttt gctgaagagg ctggtagtct gctgattctg
     8581 ttggccaaag agaccacctg gatttgttcc aaatccagtt gtactagttc caaaggcagt
     8641 tttgttctga ccatatgcaa agcctgaatt agtggtggag gagctgaaga gtcctgttgc
     8701 gctggaagtg gctggagaag acccaaacaa gccagttgtg gtacctgctc ccacctggtt
     8761 ctgtgggccc ttcctgttag cctgataatc ctctaaacga agttcctcta gtgacttgct
     8821 ttcatattct ttcatagcag taatacactg gtgcttggta cttatgttag tgctaactcc
     8881 agctttgacc atagtatctg taccagttgg agggttaaat ttaatagtag tcccagtagg
     8941 agcagctgta aaactacttg gcccaaagag ggagccagat gtgctgccaa aaggattaga
     9001 ggtggtattt gtggttccaa agagtcctcc actgctagta ctggttccaa aattgccaaa
     9061 gccagttggt ttattttgtg caaaggcatt gttttgggat gagaagagac tggtccctgt
     9121 gcttgcagtt ccaaacaagg tatttgctgt tcctgttgac gtaccaaacc caaagccagt
     9181 gcttgtggag gtagctggct ggctaaatga actggttcca aacaatcctc ctggtttagt
     9241 ctgtgaattt ccaaagaggc ctccagtatt gttgctagaa ccaaatgcag atgttccaaa
     9301 tgcccctcca ctagtagtgc caaagccagt attctgtcca aatgttgaag ttgtgccaaa
     9361 gccacctgtg ccacccccaa agggtgttcc aaatgatttg ttaaacatgg ttcgaactcc
     9421 ttccaagcct gcttttttgt acaaacttgt ttgataacct cgagagatct aattccggcg
     9481 cctagagaag gagtgagggc tggataaagg gaggatcgag gcggggtcga acgaggaggt
     9541 tcaaggggga gagacggggc ggatggagga agaggaggcg gaggcttagg gtgtacaaag
     9601 ggcttgaccc agggaggggg gtcaaaagcc aaggcttccc aggtcacgat gtaggggacc
     9661 tggtctgggt gtccatgcgg gccaggtgaa aagaccttga tcttaacctg ggtgatgagg
     9721 tctcggttaa aggtgccgtc tcgcggccat ccgacgttaa aggttggcca ttctgcagag
     9781 cagaaggtaa cccaacgtct cttcttgaca tctaccgact ggttgtgagc gatccgctcg
     9841 acatctttcc agtgacctaa ggtcaaactt aagggagtgg taacagtctg gccctaattt
     9901 tcagacaaat acagaaacac agtcagacag agacaacaca gaacgatgct gcagcgctgc
     9961 agcagacaag acgcgcggct tcggttccaa accgaaagca aaaattcaga cggaggcggg
    10021 aactgtttta ggttctcgtc tcctaccaga accacatatc ctgacggggt cggattccac
    10081 atcgactccc ttcctcaggt cgggccacaa aaacggcccc caaagtccct gggacgtctc
    10141 ccagggttgc ggccgggtgt tcagaactcg tcagttccac cacgggtccg ccagatacag
    10201 agctagttag ctaactagta cagacgcagg cgcaaacatt agatgccggc acaaacacgc
    10261 acaaagacag agacagacac gaaacgaccg ctggccagct tacctcccgg cgggggggtc
    10321 ggtggtccct gggcaggggt ctccaaatct cggtggaacc tccaaatgaa agacccccga
    10381 ggtgggcagt caatcaatct gaggagaccc tcccaaggat cagcgagtcc acgattcgga
    10441 tgcaaacagc aagaggcttt attgggaata cgggtacccg ggcgacgcag tctatcggag
    10501 gactggcgcg ccgagtgagg ggttgtgggc tcttttattg agctcgggga gcagaagcgc
    10561 gcgaacagaa gcgagaagcg aactgattgg ttagttcaaa taaggcacag ggtcatttca
    10621 ggtccttggg gcaccctgga aacatctgat ggttctctag aaactgctga gggcgggacc
    10681 gcatctgggg accatctgtt cttggccctg agccggggca ggaactgctt accacagata
    10741 tcctgtttgg cccatattct gctgtctctc tgttcctaac cttgatctga acttctctat
    10801 tctcagttat gtattttcca tgccttgcaa aatggcgtta cttaagctag cttgccaaac
    10861 ctacaggtgg ggtctttcat tccccccttt ttctggagac taaataaaat cttttatttt
    10921 atctatggct cgtactctat aggcttcagc tggtgatatt gttgagtcaa aactagagcc
    10981 tggaccactg atatcctgtc tttaacaaat tggactaatc gcctctacgc cggacgcatc
    11041 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc
    11101 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg
    11161 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt
    11221 gcgccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
    11281 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
    11341 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
    11401 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
    11461 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
    11521 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
    11581 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
    11641 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
    11701 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
    11761 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
    11821 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
    11881 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgag
//
