LOCUS       sequence-475541-       12240 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express NUP98::NSD1 in bicistronic retroviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12240)
  AUTHORS   Michmerhuizen NL, Heikamp EB, Iacobucci I, Umeda M, Arthur B, Mishra
            V, Park CS, Di Giacomo D, Hiltenbrand R, Gao Q, Radko-Juettner S, 
            Lott J, Martucci C, Subramanyam V, Hatton C, Wenge DV, Baviskar P, 
            Portola P, Claquin A, Chandra B, Baggett DW, Khalighifar A, Huang H,
            Zhou P, Long L, Shi H, Sun Y, Papachristou EK, Sekhar Reddy 
            Chilamakuri C, de Luna Vitorino FN, Gongora JM, Wu H, Pounds SB, 
            Janke LJ, Kentsis A, D'Santos CS, Garcia BA, Kriwacki RW, Chi H, 
            Klco JM, Armstrong SA, Mullighan CG
  TITLE     KAT6A and KAT7 Histone Acetyltransferase Complexes Are Molecular 
            Dependencies and Therapeutic Targets in NUP98-Rearranged Acute 
            Myeloid Leukemia.
  JOURNAL   Cancer Discov. 2025 Jun 19. doi: 10.1158/2159-8290.CD-24-1772.
  PUBMED    40536430
REFERENCE   2  (bases 1 to 12240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2025 Jun 19. doi: 10.1158/2159-8290.CD-24-1772."
COMMENT     Sequence Label: CL20_MSCV-NUP98::NSD1-ires-GFP
FEATURES             Location/Qualifiers
     source          1..12240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     139..158
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(377..481)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     549..567
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(605..627)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     727..746
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     940..962
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1090..1109
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             1309..1825
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    1889..2230
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             2297..2713
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     2643..2665
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     2681..2697
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    2740..2764
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8504..8528)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    8541..9108
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    8558..9110
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8702..8719)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8929..8948
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(9122..9124,9125..9127,9128..9841)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9167..9188)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9428..9447)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9775..9796
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             9943..10457
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    10619..10635
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10643..10649,10650..10667,10668..10673))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10688..10709
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10826..10843)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10997..11585)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11077..11096)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11756..12240,1..307,308..376))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
       61 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
      121 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
      181 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
      241 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
      301 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
      361 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
      421 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
      481 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
      541 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
      601 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
      661 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
      721 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
      781 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact
      841 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
      901 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
      961 cgacggcgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg
     1021 gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc
     1081 ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat
     1141 gccggccacg atgcgtccgg cgtagaggcg attagtccaa tttgttaaag acaggatatc
     1201 agtggtccag gctctagttt tgactcaaca atatcaccag ctgaagccta tagagtacga
     1261 gccatagata aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc
     1321 cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaataca
     1381 taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca gaatatgggc
     1441 caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc
     1501 cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc
     1561 ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct
     1621 tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc
     1681 gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata aagcctcttg
     1741 ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca gattgattga
     1801 ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga cccctgccca
     1861 gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg tgtctgtctc
     1921 tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt actagttagc
     1981 taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa cacccggccg
     2041 caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga cctgaggaag
     2101 ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag acgagaacct
     2161 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa gccgcgcgtc
     2221 ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg tgtttctgta
     2281 tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct taggtcactg
     2341 gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga gacgttgggt
     2401 taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag acggcacctt
     2461 taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc cgcatggaca
     2521 cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc cccctccctg
     2581 ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg ccccgtctct
     2641 cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag ccctcactcc
     2701 ttctctaggc gccggaatta gatctctcga ggttatcaaa caagtttgta caaaaaagca
     2761 ggctccgcgg ccgccccctt caccatgttt aacaaatcat ttggaacacc ctttgggggt
     2821 ggcacaggtg gctttggcac aacttcaaca tttggacaga atactggctt tggcactact
     2881 agtggagggg catttggaac atctgcattt ggttctagca acaatactgg aggcctcttt
     2941 ggaaattcac agactaaacc aggaggattg tttggaacca gttcatttag ccagccagct
     3001 acctccacaa gcactggctt tgggtttggt acgtcaacag gaacagcaaa taccttgttt
     3061 ggaactgcaa gcacagggac cagtctcttc tcatcccaaa acaatgcctt tgcacaaaat
     3121 aaaccaactg gctttggcaa ttttggaacc agtactagca gtggaggact ctttggaacc
     3181 acaaatacca cctctaatcc ttttggcagc acatctggct ccctctttgg gccaagtagt
     3241 tttacagctg ctcctactgg gactactatt aaatttaacc ctccaactgg tacagatact
     3301 atggtcaaag ctggagttag cactaacata agtaccaagc accagtgtat tactgctatg
     3361 aaagaatatg aaagcaagtc actagaggaa cttcgtttag aggattatca ggctaacagg
     3421 aagggcccac agaaccaggt gggagcaggt accacaactg gcttgtttgg gtcttctcca
     3481 gccacttcca gcgcaacagg actcttcagc tcctccacca ctaattcagg ctttgcatat
     3541 ggtcagaaca aaactgcctt tggaactagt acaactggat ttggaacaaa tccaggtggt
     3601 ctctttggcc aacagaatca gcagactacc agcctcttca gcaaaccatt tggccaggct
     3661 acaaccaccc agaacactgg cttttccttt ggtaatacca gcaccatagg acagccaagc
     3721 accaacacca tgggattatt tggagtaacc caagcctcac agcctggagg tctttttggg
     3781 acagctacaa acaccagcac tgggacagca tttggaacag gaacaggtct ctttgggcag
     3841 accaatactg gatttggtgc tgttggttcg accctgtttg gcaataacaa gcttactaca
     3901 tttggaagca gcacaaccag tgcaccttca tttggtacaa ccagtggcgg gctctttggt
     3961 tttggcacaa ataccagtgg gaatagtatt tttggaagta aaccagcacc tgggactctt
     4021 ggaactgggc ttggtgcagg atttggaaca gctcttggtg ctggacaggc atctttgttt
     4081 gggaacaacc aacctaagat tggagggcct cttggtacag gagcctttgg ggcccctgga
     4141 tttaatacta cgacagccac tttgggcttt ggagcccccc aggccccagt agctgtgcgg
     4201 tcagagaaga aacgccttag gaagccaagc aagtggcttt tggaatatac agaagaatat
     4261 gatcagatat ttgctcctaa gaaaaaacaa aagaaggtac aggagcaggt gcacaaggta
     4321 agttcccgct gtgaagagga aagccttcta gcccgaggtc gatctagtgc tcagaacaag
     4381 caggtggacg agaattcttt gatttcaacc aaagaagagc ctccagttct tgaaagggag
     4441 gctccgtttt tggagggccc cttggctcag tcagaacttg gaggtggaca tgctgagttg
     4501 ccgcagctga ccttgtctgt gcctgtggct ccggaagtct ctccacggcc tgcccttgag
     4561 tctgaggaat tgctagttaa aacgccagga aattatgaaa gtaaacgtca aagaaaacca
     4621 actaagaaac ttcttgaatc caatgattta gaccctggat ttatgcccaa gaagggggac
     4681 cttggccttt ctaaaaagtg ctatgaagct ggtcacctgg agaatggcat aactgaatct
     4741 tgtgccacat cttattcaaa agattttggt ggaggcacta ccaagatatt tgacaagcca
     4801 aggaagcgaa aacgacagag gcatgctgca gccaagatgc agtgtaaaaa agtgaaaaat
     4861 gatgactcgt caaaagagat tccaggctca gagggagaac taatgcctca caggacggcc
     4921 acaagcccca aggagactgt tgaggaaggt gtagaacacg atcccgggat gcctgcctct
     4981 aaaaaaatgc agggtgaacg cggtggagga gctgcactca aggagaatgt ctgtcagaat
     5041 tgtgaaaaat tgggtgagct gctgttatgt gaggctcagt gctgtggggc tttccacctg
     5101 gagtgccttg gattgactga gatgccaaga ggaaaattta tctgcaatga atgtcgcaca
     5161 ggaatccata cctgttttgt atgtaagcag agtggggaag atgttaaaag gtgccttcta
     5221 cccttgtgtg gaaagtttta ccatgaagag tgtgtccaga agtacccacc cactgttatg
     5281 cagaacaagg gcttccggtg ctccctccac atctgtataa cctgtcatgc tgctaatcca
     5341 gccaatgttt ctgcatctaa aggtcggttg atgcgctgtg tccgctgtcc tgtggcatac
     5401 cacgccaatg acttttgcct ggctgctggg tcaaagatcc ttgcatctaa tagtatcatc
     5461 tgccctaatc actttacccc taggcggggc tgccgaaatc atgagcatgt taatgttagc
     5521 tggtgctttg tgtgctcaga aggaggcagc cttctgtgct gtgattcttg ccctgctgct
     5581 tttcatcgtg aatgcctgaa cattgatatc cctgaaggaa actggtattg caatgactgt
     5641 aaagcaggca aaaagccaca ctacagggag attgtctggg taaaagttgg acgatacagg
     5701 tggtggccag ctgagatctg ccatcctcga gctgttcctt ccaacattga taagatgaga
     5761 catgatgtgg gagagttccc agtcctcttt tttggatcta atgactattt gtggactcac
     5821 caggcccgag tcttccctta catggagggt gacgtgagca gcaaggataa gatgggcaaa
     5881 ggagtggatg ggacatataa aaaagctctt caggaagctg cagcaaggtt tgaggaatta
     5941 aaggcccaaa aagagctaag acagctgcag gaagaccgaa agaatgacaa gaagccacca
     6001 ccttataaac atataaaggt aaaccgtcct attggcaggg tacagatctt cactgcagac
     6061 ttatctgaaa taccccgttg caactgtaaa gctactgatg agaacccctg tgggatagac
     6121 tctgaatgca tcaaccgcat gctgctctat gagtgccacc ccacagtgtg tcctgccgga
     6181 gggcgctgtc aaaaccagtg cttttccaag cgccaatatc cagaggttga aattttccgc
     6241 acattacagc ggggttgggg tctacggaca aaaacagata ttaaaaaggg tgaatttgtg
     6301 aatgagtatg tgggtgagct tatagatgaa gaagaatgca gagctcgaat tcgctatgct
     6361 caagaacatg atatcactaa tttctatatg ctcaccctag acaaagaccg aatcattgat
     6421 gctggtccca aaggaaacta tgctcggttc atgaatcatt gctgccagcc caactgtgaa
     6481 acacagaagt ggtctgtgaa tggagatacc cgtgtaggcc tttttgcact aagtgacatt
     6541 aaagcaggca ctgaacttac cttcaactac aacctagaat gtcttgggaa tggaaagact
     6601 gtttgcaaat gtggagcccc gaactgcagt ggcttcttgg gtgtaaggcc aaagaatcaa
     6661 cccattgcca cggaagaaaa gtcaaagaaa ttcaagaaga agcaacaggg aaagcgcagg
     6721 acccagggtg aaatcacaaa ggagcgagaa gatgagtgtt ttagttgtgg ggatgctggc
     6781 cagctcgtct cctgcaagaa accaggctgc ccaaaagttt accacgcaga ctgtctcaat
     6841 ctgaccaagc gaccagcagg gaaatgggaa tgtccgtggc atcagtgtga catctgcggg
     6901 aaggaagcag cctccttctg tgagatgtgc cccagctcct tttgtaagca gcatcgagaa
     6961 gggatgcttt tcatttccaa actggatggg cgtctgtctt gtactgagca tgacccctgt
     7021 gggcccaatc ctctggaacc tggggagatc cgtgagtatg tgcctccccc agtaccgctg
     7081 cctccagggc caagcactca cctggcagag caatcaacag gaatggctgc tcaggcaccc
     7141 aaaatgtcag ataaacctcc tgctgacacc aaccagatgc tgtcgctctc caaaaaagct
     7201 ctggcaggga cttgtcagag gccattgcta cctgaaagac ctcttgagag aactgactcc
     7261 aggccccagc ctttagataa ggtcagagac ctcgctgggt cagggaccaa atcccaatcc
     7321 ttggtttcca gccagaggcc actggacagg ccaccagcag tggcaggacc aagaccccag
     7381 ctaagcgaca aaccctctcc agtgaccagc ccaagctcct caccctcagt caggtcccaa
     7441 ccactggaaa gacctctggg gacggctgac ccaaggctgg ataaatccat aggtgctgcc
     7501 agcccaaggc cccagtcact ggagaaaacc tcagttccca ctggcctgag acttccgccg
     7561 ccagacagac tgctcattac tagcagtccc aaaccccaga cttcagacag gcctactgac
     7621 aaaccccatg cctctttgtc ccagagactc ccacctcctg agaaagtact atcagctgtg
     7681 gtccagaccc ttgtagctaa agaaaaagca ctgaggcctg tggaccagaa tactcagtca
     7741 aaaaatagag ctgctttggt gatggatctc atagacctaa ctcctcgcca gaaggagcgg
     7801 gcagcttcac ctcatcaggt cacaccacag gctgatgaga agatgccagt gttggagtca
     7861 agttcatggc ctgccagcaa aggtctgggg catatgccga gagctgttga gaaaggctgt
     7921 gtgtcagatc ctcttcagac atctgggaaa gcagcagccc cttcagagga cccctggcaa
     7981 gctgttaaat cactcaccca ggccagactt ctttctcagc ctcctgccaa ggccttttta
     8041 tatgagccaa caactcaggc ctcaggaaga gcttctgcag gggctgagca gaccccaggg
     8101 cctcttagcc aatccccggg cctggtgaag caggcgaagc agatggtcgg aggccagcaa
     8161 ctacctgcac ttgccgccaa gagtgggcaa tcttttaggt ctctcgggaa ggccccagcc
     8221 tccctcccca ctgaagaaaa gaagttggta accacagagc aaagtccctg ggccctggga
     8281 aaagcctcat cacgggcagg gctctggccc atagtggctg gacagacact ggcacagtct
     8341 tgctggtctg ctgggagcac acagacattg gcacagactt gctggtctct tggaagaggg
     8401 caagacccca aaccagagca aaatacactt ccagctctta accaggctcc ttccagtcac
     8461 aagtgtgcag aatcagaaca gaagtagaag ggtgggcgcg ccgacccagc tttcttgtac
     8521 aaagtggttc gataacgaat tccgcccccc ccccctaacg ttactggccg aagccgcttg
     8581 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
     8641 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
     8701 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa
     8761 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
     8821 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
     8881 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
     8941 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
     9001 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
     9061 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac
     9121 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     9181 cggcgacgta aacggccaca agttcagcgt gtctggcgag ggcgagggcg atgccaccta
     9241 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     9301 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     9361 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     9421 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     9481 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     9541 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     9601 cggcatcaag gcgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     9661 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     9721 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     9781 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     9841 atgaattaat taagaattat caagcttatc gataccgtcg acctgcagcc aagcttatcg
     9901 ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt
     9961 aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaat acataactga
    10021 gaatagagaa gttcagatca aggttaggaa cagagagaca gcagaatatg ggccaaacag
    10081 gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat
    10141 gcggtcccgc cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga
    10201 cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc
    10261 gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc ggcgcgccag
    10321 tcctccgata gactgcgtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc
    10381 atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt
    10441 cagcgggggt ctttcatggg taacagtttc ttgaagttgg agaacaacat tctgagggta
    10501 ggagtcgaat attaagtaat cctgactcaa ttagccactg ttttgaatcc acatactcca
    10561 atactcctga aatagttcat tatggacagc gcagaaagag ctggggagaa ttgtgaaatt
    10621 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
    10681 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
    10741 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
    10801 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
    10861 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
    10921 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
    10981 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
    11041 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
    11101 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
    11161 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
    11221 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    11281 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    11341 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    11401 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
    11461 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    11521 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    11581 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    11641 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    11701 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    11761 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    11821 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    11881 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    11941 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    12001 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    12061 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    12121 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    12181 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
//
